Articles

Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application

Excessive application of chemical fertilizer has exerted a great threat to soil quality and the environment. The inoculation of plants with plant-growth-promoting rhizobacteria (PGPR) has emerged as a great pr...

Authors: Juanjuan Wang, Ruochen Li, Hui Zhang, Gehong Wei and Zhefei Li

PilG and PilH antagonistically control flagellum-dependent and pili-dependent motility in the phytopathogen Xanthomonas campestris pv. campestris

The virulence of the plant pathogen Xanthomonas campestris pv. campestris (Xcc) involves the coordinate expression of many virulence factors, including surface appendages flagellum and type IV pili, which are req...

Authors: Yan-Hua Qi, Li Huang, Guo-Fang Liu, Ming Leng and Guang-Tao Lu

Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron...

Authors: Tao Chen, Guofeng Dong, Siqin Zhang, Xiucai Zhang, Yajie Zhao, Jianming Cao, Tieli Zhou and Qing Wu

Identification of promoter elements in the Dolichospermum circinale AWQC131C saxitoxin gene cluster and the experimental analysis of their use for heterologous expression

Dolichospermum circinale is a filamentous bloom-forming cyanobacterium responsible for biosynthesis of the paralytic shellfish toxins (PST), including saxitoxin. PSTs are neurotoxins and in their purified form ar...

Authors: Paul M. D’Agostino, Bakir Al-Sinawi, Rabia Mazmouz, Julia Muenchhoff, Brett A. Neilan and Michelle C. Moffitt

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl sulfate surfactants. Toxicological information on SDS is accumulating, however, mechanisms of SDS toxicity regulation remain poorly und...

Authors: Chunlei Cao, Zhengfeng Cao, Peibin Yu and Yunying Zhao

Diversity and geographic distribution of soil streptomycetes with antagonistic potential against actinomycetoma-causing Streptomyces sudanensis in Sudan and South Sudan

Production of antibiotics to inhibit competitors affects soil microbial community composition and contributes to disease suppression. In this work, we characterized whether Streptomyces bacteria, prolific antibio...

Authors: Mohamed E. Hamid, Thomas Reitz, Martin R. P. Joseph, Kerstin Hommel, Adil Mahgoub, Mogahid M. Elhassan, François Buscot and Mika Tarkka

Characterization and whole genome sequencing of a novel strain of Bergeyella cardium related to infective endocarditis

Bergeyella cardium infection is becoming increasingly prevalent in patients with infective endocarditis, suggesting its significance in disease pathogenesis. However, few studies have fully characterized this spe...

Authors: Hongwei Pan, Wei Li, Enhua Sun and Yi Zhang

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Acinetobacter baumannii is a multidrug-resistant (MDR) hazardous bacterium with very high antimicrobial resistance profiles. Outer membrane vesicles (OMVs) help directly and/or indirectly towards antibiotic resis...

Authors: DineshKumar Kesavan, Aparna Vasudevan, Liang Wu, Jianguo Chen, Zhaoliang Su, Shengjun Wang and Huaxi Xu

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enteroc...

Authors: Jinxin Zheng, Yang Wu, Zhiwei Lin, Guangfu Wang, Sibo Jiang, Xiang Sun, Haopeng Tu, Zhijian Yu and Di Qu

Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs

Many recent studies have gravitated towards manipulating the gastrointestinal (GI) microbiome of livestock to improve host nutrition and health using dietary interventions. Few studies, however, have evaluated...

Authors: Shaobo Yu, Guangyu Zhang, Zhibo Liu, Peng Wu, Zhongtang Yu and Jiakun Wang

Synergistic effects of vorinostat (SAHA) and azoles against Aspergillus species and their biofilms

Invasive aspergillosis is a fungal infection that occurs mainly in immunocompromised patients. It is responsible for a high degree of mortality and is invariably unresponsive to conventional antifungal treatme...

Authors: Bo Tu, Gendi Yin and Hui Li

Epidemic ribotypes of Clostridium (now Clostridioides) difficile are likely to be more virulent than non-epidemic ribotypes in animal models

Clostridioides difficile infections have become more frequently diagnosed and associated with greater disease severity, which has resulted in an increase burden on the healthcare system. These increases are attri...

Authors: John C. Vitucci, Mark Pulse, Leslie Tabor-Simecka and Jerry Simecka

Identification of E2 with improved secretion and immunogenicity against CSFV in piglets

Outbreaks of Classical swine fever virus (CSFV) cause significant economic losses in the swine industry. Vaccination is the major method to prevent and control the disease. As live attenuated vaccines fail to ...

Authors: Huiling Xu, Yanli Wang, Guangwei Han, Weihuan Fang and Fang He

Design of targeted primers based on 16S rRNA sequences in meta-transcriptomic datasets and identification of a novel taxonomic group in the Asgard archaea

Amplification of small subunit (SSU) rRNA genes with universal primers is a common method used to assess microbial populations in various environmental samples. However, owing to limitations in coverage of the...

Authors: Ru-Yi Zhang, Bin Zou, Yong-Wei Yan, Che Ok Jeon, Meng Li, Mingwei Cai and Zhe-Xue Quan

Fermentation optimization and disease suppression ability of a Streptomyces ma. FS-4 from banana rhizosphere soil

Fusarium wilt of banana is one of the most destructive diseases in banana-growing regions worldwide. Soil-borne diseases and soil microbial communities are closely related. The screening of antagonistic bacteria ...

Authors: Yajie Duan, Jian Chen, Wei He, Jingjing Chen, Zhencai Pang, Huigang Hu and Jianghui Xie

The C3HC type zinc-finger protein (ZFC3) interacting with Lon/MAP1 is important for mitochondrial gene regulation, infection hypha development and longevity of Magnaporthe oryzae

The rice blast is a typical fungal disease caused by Magnaporthe oryzae, and the mitochondrial ATP-dependent Lon protease (MAP1) has been proven to be involved in blast development. We previously screened a C3HC ...

Authors: Shaoshuai Liu, Yi Wei and Shi-Hong Zhang

A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms

Staphylococcus aureus is a human pathogen that is a common cause of nosocomial infections and infections on indwelling medical devices, mainly due to its ability to shift between the planktonic and the biofilm/se...

Authors: Aizat Iman Abdul Hamid, Laurence Nakusi, Mickael Givskov, Young-Tae Chang, Claire Marquès and Pascale Gueirard

High concentration of coagulase-negative staphylococci carriage among bioaerosols of henhouses in Central China

Coagulase-negative staphylococci (CoNS) are a group of opportunistic pathogens, which are widely spread in the environment. Animal breeding is an important source of pathogen spreading. However, the concentrat...

Authors: Yuanqing Lu, Qin Lu, Yiluo Cheng, Guoyuan Wen, Qingping Luo, Huabin Shao and Tengfei Zhang

Comparative genomics of multidrug-resistant Enterococcus spp. isolated from wastewater treatment plants

Wastewater treatment plants (WWTPs) are considered hotspots for the environmental dissemination of antimicrobial resistance (AMR) determinants. Vancomycin-Resistant Enterococcus (VRE) are candidates for gauging t...

Authors: Haley Sanderson, Rodrigo Ortega-Polo, Rahat Zaheer, Noriko Goji, Kingsley K. Amoako, R. Stephen Brown, Anna Majury, Steven N. Liss and Tim A. McAllister

Marine bacterial communities in the upper gulf of Thailand assessed by Illumina next-generation sequencing platform

The total bacterial community plays an important role in aquatic ecosystems. In this study, bacterial communities and diversity along the shores of the Upper Gulf of Thailand were first characterized. The asso...

Authors: Pongrawee Nimnoi and Neelawan Pongsilp

An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151–6 and Bacillus marisflavi 151–25

Cadmium (Cd) is a severely toxic heavy metal to most microorganisms. Many bacteria have developed Cd²⁺ resistance.

Authors: Xiaoxia Yu, Zundan Ding, Yangyang Ji, Jintong Zhao, Xiaoqing Liu, Jian Tian, Ningfeng Wu and Yunliu Fan

Low levels of HIV-1 drug resistance mutations in patients who achieved viral re-suppression without regimen switch: a retrospective study

We identified a HIV-positive cohort in virologic failure (VF) who re-suppressed without drug switch. We characterized their drug resistance mutations (DRM) and adherence profiles to learn how to better manage ...

Authors: Chika K. Onwuamah, Jonathan Okpokwu, Rosemary Audu, Godwin Imade, Seema T. Meloni, Azuka Okwuraiwe, Philippe Chebu, Adesola Z. Musa, Beth Chaplin, Ibrahim Dalhatu, Oche Agbaji, Jay Samuels, Oliver Ezechi, Mukhtar Ahmed, Georgina Odaibo, David O. Olaleye…

Pneumococcal serotype determines growth and capsule size in human cerebrospinal fluid

The polysaccharide capsule is a major virulence factor of S. pneumoniae in diseases such as meningitis. While some capsular serotypes are more often found in invasive disease, high case fatality rates are associa...

Authors: Annelies Müller, Anke Salmen, Suzanne Aebi, Linda de Gouveia, Anne von Gottberg and Lucy J. Hathaway

Sampling methods affect Nematode-Trapping Fungi biodiversity patterns across an elevational gradient

Understanding the patterns of species richness across elevational gradients is a key concept for contemporary research in ecology and evolution, and critical to understanding large-scale trends in biodiversity...

Authors: Wei Deng, Jia-Liang Wang, Matthew B. Scott, Yi-Hao Fang, Shuo-Ran Liu, Xiao-Yan Yang and Wen Xiao

Prevalence and antimicrobial susceptibility of enterotoxigenic extra-intestinal Bacteroides fragilis among 13-year collection of isolates in Kuwait

Some strains of Bacteroides fragilis species are associated with diarrhea as a result of enterotoxin production (bft or fragilysin). Fragilysin is activated by C11 protease (fpn) and together with C10 protease (b...

Authors: Wafaa Jamal, Fatima Bibi Khodakhast, Ameerah AlAzmi, Jόzsef Sόki, Ghayda AlHashem and Vincent O. Rotimi

Genome-resolved metagenomics analysis provides insights into the ecological role of Thaumarchaeota in the Amazon River and its plume

Thaumarchaeota are abundant in the Amazon River, where they are the only ammonia-oxidizing archaea. Despite the importance of Thaumarchaeota, little is known about their physiology, mainly because few isolates...

Authors: Otávio H. B. Pinto, Thais F. Silva, Carla S. Vizzotto, Renata H. Santana, Fabyano A. C. Lopes, Bruno S. Silva, Fabiano L. Thompson and Ricardo H. Kruger

Comparisons of microbiological characteristics and antibiotic resistance of Klebsiella pneumoniae isolates from urban rodents, shrews, and healthy people

The comparisons of molecular characterization and antibiotic resistance of Klebsiella pneumoniae (KP) isolates from humans and other animal hosts are not well studied. Our goal was to compare the molecular epidem...

Authors: Xue-shan Zhong, Yong-zhi Li, Jing Ge, Gang Xiao, Yun Mo, Yu-qi Wen, Jing-ping Liu, Yi-quan Xiong, Min Qiu, Shu-ting Huo, Ming-ji Cheng and Qing Chen

Biodegradation of endocrine disruptor Bisphenol A by Pseudomonas putida strain YC-AE1 isolated from polluted soil, Guangdong, China

Bisphenol A is an important organic chemical as an intermediate, final and inert ingredient in manufacturing of many important products like polycarbonate plastics, epoxy resins, flame retardants, food–drink p...

Authors: Adel Eltoukhy, Yang Jia, Ruth Nahurira, M. A. Abo-Kadoum, Ibatsam Khokhar, Junhuan Wang and Yanchun Yan

First identification and genotyping of Enterocytozoon bieneusi in humans in Myanmar

Intestinal pathogen infections are widespread among impoverished populations. Enterocytozoon bieneusi is the most common pathogen of intestinal microsporidian species in humans worldwide. However, no epidemiologi...

Authors: Yujuan Shen, Baiyan Gong, Xiaohua Liu, Yanchen Wu, Fengkun Yang, Jie Xu, Xiaofan Zhang, Jianping Cao and Aiqin Liu

Correction to: Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene

After publication of our article [1] it came to our notice that the source of the sequence for the control plasmid, pNeo (Materials and methods: Controls) was incorrectly stated as AB094461. The correct access...

Authors: Andrew Jenkins, Cecilie Raasok, Benedikte N. Pedersen, Kristine Jensen, Åshild Andreassen, Arnulf Soleng, Kristin Skarsfjord Edgar, Heidi Heggen Lindstedt, Vivian Kjelland, Snorre Stuen, Dag Hvidsten and Bjørn-Erik Kristiansen

Larva of greater wax moth Galleria mellonella is a suitable alternative host for the fish pathogen Francisella noatunensis subsp. orientalis

Francisella noatunensis subsp. orientalis (Fno) is the etiological agent of francisellosis in cultured warm water fish, such as tilapia. Antibiotics are administered to treat the disease but a better understandin...

Authors: Winarti Achmad Sarmin Djainal, Khalid Shahin, Matthijs Metselaar, Alexandra Adams and Andrew P. Desbois

Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria

Colistin resistance is considered a serious problem due to a lack of alternative antibiotics. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is a resazurin reduction-based technique that relies on the ...

Authors: Huaiyu Jia, Renchi Fang, Jie Lin, Xuebin Tian, Yajie Zhao, Lijiang Chen, Jianming Cao and Tieli Zhou

Nucleotide polymorphism assay for the identification of west African group Bacillus anthracis: a lineage lacking anthrose

The exosporium of the anthrax-causing Bacillus anthracis endospores display a tetrasaccharide composed of three rhamnose residues and an unusual sugar termed anthrose. Anthrose is a proposed potential target for ...

Authors: Diansy Zincke, Michael H. Norris, Berzhan Kurmanov, Ted L. Hadfield and Jason K. Blackburn

In vitro synergy between sodium deoxycholate and furazolidone against enterobacteria

Antimicrobial combinations have been proven as a promising approach in the confrontation with multi-drug resistant bacterial pathogens. In the present study, we identify and characterize a synergistic interact...

Authors: Vuong Van Hung Le, Catrina Olivera, Julian Spagnuolo, Ieuan G. Davies and Jasna Rakonjac

HpaXpm, a novel harpin of Xanthomonas phaseoli pv. manihotis, acts as an elicitor with high thermal stability, reduces disease, and promotes plant growth

Harpins are proteins secreted by the type III secretion system of Gram-negative bacteria during pathogen–plant interactions that can act as elicitors, stimulating defense and plant growth in many types of non-...

Authors: Yue Liu, Xiaoyun Zhou, Wenbo Liu, Jiamin Huang, Qinghuan Liu, Jianzhang Sun, Xinfeng Cai and Weiguo Miao

Temporal analysis of mRNA expression profiles in Orientia infected C3HeB/FeJ mouse

Scrub typhus causes up to 35% mortality if left untreated. One billion people living in the endemic regions are at risk. In spite of its heavy disease burden in some of the most populated areas in the world, t...

Authors: Chien-Chung Chao, Ruoting Yang, Zhiwen Zhang, Tatyana Belinskaya, Chye-Teik Chan, Stacy-Ann Miller, Rasha Hammamieh, Marti Jett and Wei-Mei Ching

Method comparison for the direct enumeration of bacterial species using a chemostat model of the human colon

Clostridioides difficile infection (CDI) has a high recurrent infection rate. Faecal microbiota transplantation (FMT) has been used successfully to treat recurrent CDI, but much remains unknown about the human gu...

Authors: Ines B. Moura, Charmaine Normington, Duncan Ewin, Emma Clark, Mark H. Wilcox, Anthony M. Buckley and Caroline H. Chilton

An improved bind-n-seq strategy to determine protein-DNA interactions validated using the bacterial transcriptional regulator YipR

Interactions between transcription factors and DNA lie at the centre of many biological processes including DNA recombination, replication, repair and transcription. Most bacteria encode diverse proteins that ...

Authors: Shi-qi An, Miguel A. Valvano, Yan-hua Yu, Jeremy S. Webb and Guillermo Lopez Campos

Long-term impact of fecal transplantation in healthy volunteers

Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies inv...

Authors: Oleg V. Goloshchapov, Evgenii I. Olekhnovich, Sergey V. Sidorenko, Ivan S. Moiseev, Maxim A. Kucher, Dmitry E. Fedorov, Alexander V. Pavlenko, Alexander I. Manolov, Vladimir V. Gostev, Vladimir A. Veselovsky, Ksenia M. Klimina, Elena S. Kostryukova, Evgeny A. Bakin, Alexander N. Shvetcov, Elvira D. Gumbatova, Ruslana V. Klementeva…

Gut bacteria of weevils developing on plant roots under extreme desert conditions

Many phytophagous insects, whose diet is generally nitrogen-poor, rely on gut bacteria to compensate for nutritional deficits. Accordingly, we hypothesized that insects in desert environments may evolve associ...

Authors: Fengqun Meng, Nitsan Bar-Shmuel, Reut Shavit, Adi Behar and Michal Segoli

An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics

The majority of in vitro studies of medically relevant biofilms involve the development of biofilm on an inanimate solid surface. However, infection in vivo consists of biofilm growth on, or suspended within, ...

Authors: Elisabeth A. Slade, Robin M. S. Thorn, Amber Young and Darren M. Reynolds

Primary progressive multiple sclerosis in a Russian cohort: relationship with gut bacterial diversity

Gut microbiota has been increasingly acknowledged to shape significantly human health, contributing to various autoimmune diseases, both intestinal and non-intestinal, including multiple sclerosis (MS). Gut mi...

Authors: Madina Kozhieva, Natalia Naumova, Tatiana Alikina, Alexey Boyko, Valentin Vlassov and Marsel R. Kabilov

Application of LpxC enzyme inhibitor to inhibit some fast-growing bacteria in human gut bacterial culturomics

Culturomics can ascertain traces of microorganisms to be cultivated using different strategies and identified by matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry or 16S rDNA sequenc...

Authors: Fengyi Hou, Yuxiao Chang, Zongyu Huang, Ni Han, Lei Bin, Huimin Deng, Zhengchao Li, Zhiyuan Pan, Lei Ding, Hong Gao, Ruifu Yang, Fachao Zhi and Yujing Bi

Composite genome sequence of Bacillus clausii, a probiotic commercially available as Enterogermina^®, and insights into its probiotic properties

Some of the spore-forming strains of Bacillus probiotics are marketed commercially as they survive harsh gastrointestinal conditions and bestow health benefits to the host.

Authors: Indu Khatri, Gaurav Sharma and Srikrishna Subramanian

Pan-genome diversification and recombination in Cronobacter sakazakii, an opportunistic pathogen in neonates, and insights to its xerotolerant lifestyle

Cronobacter sakazakii is an emerging opportunistic bacterial pathogen known to cause neonatal and pediatric infections, including meningitis, necrotizing enterocolitis, and bacteremia. Multiple disease outbreaks ...

Authors: Isaiah Paolo A. Lee and Cheryl P. Andam

Phenotypic and molecular characterization of antimicrobial resistance in Trueperella pyogenes strains isolated from bovine mastitis and metritis

Trueperella pyogenes is one of the most clinically imperative bacteria responsible for severe cases of mastitis and metritis, particularly in postpartum dairy cows. The bacterium has emergence of antibiotic resis...

Authors: Mobin Rezanejad, Sepideh Karimi and Hassan Momtaz

Variation of growth characteristics of pneumococcus with environmental conditions

Pneumococcus is exposed to a variety of temperature and oxygen levels in the upper respiratory tract and as it invades the lung, tissues, and blood. We sought to determine the effect of environmental variabili...

Authors: Adrienn Tóthpál, Katherine Desobry, Shreyas S. Joshi, Anne L. Wyllie and Daniel M. Weinberger

Enterobacter sp. AA26 gut symbiont as a protein source for Mediterranean fruit fly mass-rearing and sterile insect technique applications

Insect species have established sophisticated symbiotic associations with diverse groups of microorganisms including bacteria which have been shown to affect several aspects of their biology, physiology, ecolo...

Authors: Georgios A. Kyritsis, Antonios A. Augustinos, Spyridon Ntougias, Nikos T. Papadopoulos, Kostas Bourtzis and Carlos Cáceres

Commensal microbiota modulates larval foraging behaviour, development rate and pupal production in Bactrocera tryoni

Commensal microbes can promote survival and growth of developing insects, and have important fitness implications in adulthood. Insect larvae can acquire commensal microbes through two main routes: by vertical...

Authors: Juliano Morimoto, Binh Nguyen, Shabnam T. Tabrizi, Ida Lundbäck, Phillip W. Taylor, Fleur Ponton and Toni A. Chapman

Joint FAO/IAEA coordinated research project on “use of symbiotic bacteria to reduce mass-rearing costs and increase mating success in selected fruit pests in support of SIT application”

Authors: Carlos Cáceres, George Tsiamis, Boaz Yuval, Edouard Jurkevitch and Kostas Bourtzis