Articles

Long-term colonization exceeding six years from early infancy of Bifidobacterium longum subsp. longum in human gut

The importance of the gut microbiota at the early stage of life and their longitudinal effect on host health have recently been well investigated. In particular, Bifidobacterium longum subsp. longum, a common com...

Authors: Kaihei Oki, Takuya Akiyama, Kazunori Matsuda, Agata Gawad, Hiroshi Makino, Eiji Ishikawa, Kenji Oishi, Akira Kushiro and Junji Fujimoto

Diet, physical activity and screen time but not body mass index are associated with the gut microbiome of a diverse cohort of college students living in university housing: a cross-sectional study

Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between b...

Authors: Corrie M. Whisner, Juan Maldonado, Brandon Dente, Rosa Krajmalnik-Brown and Meg Bruening

Adaptation mechanism and tolerance of Rhodopseudomonas palustris PSB-S under pyrazosulfuron-ethyl stress

Pyrazosulfuron-ethyl is a long lasting herbicide in the agro-ecosystem and its residue is toxic to crops and other non-target organisms. A better understanding of molecular basis in pyrazosulfuron-ethyl tolera...

Authors: Xiang-Wen Luo, De-Yang Zhang, Teng-Hui Zhu, Xu-Guo Zhou, Jing Peng, Song-Bai Zhang and Yong Liu

Role of phage ϕ1 in two strains of Salmonella Rissen, sensitive and resistant to phage ϕ1

The study describes the Salmonella Rissen phage ϕ1 isolated from the ϕ1-sensitive Salmonella Rissen strain R^W. The same phage was then used to select the resistant strain R^Rϕ1+, which can harbour or not ϕ1.

Authors: Marina Papaianni, Felice Contaldi, Andrea Fulgione, Sheridan L. Woo, Angela Casillo, Maria Michela Corsaro, Ermenegilda Parrilli, Luca Marcolungo, Marzia Rossato, Massimo Delledonne, Marianna Garonzi, Domenico Iannelli and Rosanna Capparelli

USA300 Staphylococcus aureus persists on multiple body sites following an infection

USA300 methicillin-resistant Staphylococcus aureus (MRSA) is a community- and hospital-acquired pathogen that frequently causes infections but also can survive on the human body asymptomatically as a part of the ...

Authors: Timothy D. Read, Robert A. Petit III, Zachary Yin, Tuyaa Montgomery, Moira C. McNulty and Michael Z. David

Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia

Mannheimia haemolytica has been recognized as the principal cause of pneumonic pasteurellosis in sheep and goats. It is one of the important diseases of small ruminants in Ethiopia. While annual vaccination using...

Authors: Abinet Legesse, Takele Abayneh, Gezahegne Mamo, Esayas Gelaye, Liyuwork Tesfaw, Martha Yami and Alebachew Belay

The possible molecular mechanisms of farnesol on the antifungal resistance of C. albicans biofilms: the regulation of CYR1 and PDE2

Farnesol has potential antifungal activity against Candida albicans biofilms, but the molecular mechanism of this activity is still unclear. Farnesol inhibits hyphal growth by regulating the cyclic AMP (cAMP) sig...

Authors: Shengyan Chen, Jinping Xia, Chengxi Li, Lulu Zuo and Xin Wei

Genotyping of Coxiella burnetii in sheep and goat abortion samples

Q fever, caused by Coxiella burnetii, is a zoonosis that presents a worldwide distribution and affects both humans and animals. The route of dispersal of the pathogen by ruminants into the environment usually inv...

Authors: Dimosthenis Chochlakis, Ana Sofia Santos, Nektarios D. Giadinis, Dimitrios Papadopoulos, Leonidas Boubaris, Emmanouil Kalaitzakis, Anna Psaroulaki, Spyridon K. Kritas and Evanthia I. Petridou

Characterization of hepatitis B virus with complex structural variations

Hepatitis B virus (HBV) infection is one of the most serious public health issues. Recent HBV genetic research has revealed novel genetic rearrangements termed complex structural variations (SVs), which are co...

Authors: Kei Fujiwara, Kentaro Matsuura, Kayoko Matsunami, Etsuko Iio and Shunsuke Nojiri

Purification and partial characterization of LdtP, a cell envelope modifying enzyme in Liberibacter asiaticus

The aggressive spread of Liberibacter asiaticus, a bacterium closely associated with citrus greening, has given rise to an acute crisis in the citrus industry, making it imperative to expand the scientific knowle...

Authors: Janelle F. Coyle, Fernando A. Pagliai, Dan Zhang, Graciela L. Lorca and Claudio F. Gonzalez

The spent culture supernatant of Pseudomonas syringae contains azelaic acid

Pseudomonas syringae pv. actinidiae (PSA) is an emerging kiwifruit bacterial pathogen which since 2008 has caused considerable losses. No quorum sensing (QS) signaling molecule has yet been reported from PSA and ...

Authors: Sree Gowrinadh Javvadi, Paola Cescutti, Roberto Rizzo, Valentina Lonzarich, Luciano Navarini, Danilo Licastro, Corrado Guarnaccia and Vittorio Venturi

Random mutagenesis of super Koji (Aspergillus oryzae): improvement in production and thermal stability of α-amylases for maltose syrup production

Alpha-amylases hydrolyze 1,4 α-glycosidic bonds of starch and produce malto-oligosaccharides. It is an important enzyme generally applied in textile, food and brewing industries. Enhancement in thermal stabili...

Authors: Bushra Aleem, Muhammad Hamid Rashid, Neelam Zeb, Anam Saqib, Ayesha Ihsan, Mazhar Iqbal and Hazrat Ali

Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages

Klebsiella oxytoca DSM 29614 - isolated from acid mine drainages - grows anaerobically using Fe(III)-citrate as sole carbon and energy source, unlike other enterobacteria and K. oxytoca clinical isolates. The DSM...

Authors: Giuseppe Gallo, Luana Presta, Elena Perrin, Michele Gallo, Davide Marchetto, Anna Maria Puglia, Renato Fani and Franco Baldi

A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides

The field of diagnostics continues to advance rapidly with a variety of novel approaches, mainly dependent upon high technology platforms. Nonetheless much diagnosis, particularly in developing countries, stil...

Authors: Balkis A. Talip, William J. Snelling, Roy D. Sleator, Colm Lowery and James S. G. Dooley

The murine vaginal microbiota and its perturbation by the human pathogen group B Streptococcus

Composition of the vaginal microbiota has significant influence on female urogenital health and control of infectious disease. Murine models are widely utilized to characterize host-pathogen interactions withi...

Authors: Alison Vrbanac, Angelica M. Riestra, Alison Coady, Rob Knight, Victor Nizet and Kathryn A. Patras

Bacterial diversity obtained by culturable approaches in the gut of Glossina pallidipes population from a non sleeping sickness focus in Tanzania: preliminary results

Glossina pallidipes is a haematophagous insect that serves as a cyclic transmitter of trypanosomes causing African Trypanosomiasis (AT). To fully assess the role of G. pallidipes in the epidemiology of AT, especi...

Authors: Imna Malele, Hamis Nyingilili, Eugen Lyaruu, Marc Tauzin, B. Bernard Ollivier, Jean-Luc Cayol, Marie-Laure Fardeau and Anne Geiger

Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans)

Symbiotic microbes represent a driving force of evolutionary innovation by conferring novel ecological traits to their hosts. Many insects are associated with microbial symbionts that contribute to their host’...

Authors: Tobias Engl, Veronika Michalkova, Brian L. Weiss, Güler D. Uzel, Peter Takac, Wolfgang J. Miller, Adly M. M. Abd-Alla, Serap Aksoy and Martin Kaltenpoth

Sodalis glossinidius presence in wild tsetse is only associated with presence of trypanosomes in complex interactions with other tsetse-specific factors

Susceptibility of tsetse flies (Glossina spp.) to trypanosomes of both humans and animals has been associated with the presence of the endosymbiont Sodalis glossinidius. However, intrinsic biological characterist...

Authors: Manun Channumsin, Marc Ciosi, Dan Masiga, C Michael R Turner and Barbara K Mable

Hytrosavirus genetic diversity and eco-regional spread in Glossina species

The management of the tsetse species Glossina pallidipes (Diptera; Glossinidae) in Africa by the sterile insect technique (SIT) has been hindered by infections of G. pallidipes production colonies with Glossina p...

Authors: Irene K. Meki, Henry M. Kariithi, Mehrdad Ahmadi, Andrew G. Parker, Marc J. B. Vreysen, Just M. Vlak, Monique M. van Oers and Adly M.M. Abd-Alla

What can a weevil teach a fly, and reciprocally? Interaction of host immune systems with endosymbionts in Glossina and Sitophilus

The tsetse fly (Glossina genus) is the main vector of African trypanosomes, which are protozoan parasites that cause human and animal African trypanosomiases in Sub-Saharan Africa. In the frame of the IAEA/FAO pr...

Authors: Anna Zaidman-Rémy, Aurélien Vigneron, Brian L Weiss and Abdelaziz Heddi

Witches’ broom resistant genotype CCN51 shows greater diversity of symbiont bacteria in its phylloplane than susceptible genotype catongo

Theobroma cacao L. (cacao) is a perennial tropical tree, endemic to rainforests of the Amazon Basin. Large populations of bacteria live on leaf surfaces and these phylloplane microorganisms can have important eff...

Authors: Juliano Oliveira Santana, Karina Peres Gramacho, Katiúcia Tícila de Souza Eduvirgens Ferreira, Rachel Passos Rezende, Pedro Antônio Oliveira Mangabeira, Ricardo Pedro Moreira Dias, Francisco M. Couto and Carlos Priminho Pirovani

Blood feeding tsetse flies as hosts and vectors of mammals-pre-adapted African Trypanosoma: current and expected research directions

Research on the zoo-anthropophilic blood feeding tsetse flies’ biology conducted, by different teams, in laboratory settings and at the level of the ecosystems- where also co-perpetuate African Trypanosoma- has a...

Authors: Anne Geiger, Imna Malele, Adly M Abd-Alla and Flobert Njiokou

Low recovery of bacterial community after an extreme salinization-desalinization cycle

Understanding the recovery of bacterial communities after extreme environmental disturbances offers key opportunities to investigate ecosystem resilience. However, it is not yet clear whether bacterial communi...

Authors: Yang Hu, Chengrong Bai, Jian Cai, Keqiang Shao, Xiangming Tang and Guang Gao

Gene expression in reproductive organs of tsetse females – initial data in an approach to reduce fecundity

Tsetse flies are vectors of African trypanosomes, and their vectorial capacity results in a major public health emergency and vast economic losses in sub-Saharan Africa. Given the limited ability of trypanosom...

Authors: Emanuel Procházka, Veronika Michalková, Ivana Daubnerová, Ladislav Roller, Peter Klepsatel, Dušan Žitňan, George Tsiamis and Peter Takáč

Prevalence of trypanosomes, salivary gland hypertrophy virus and Wolbachia in wild populations of tsetse flies from West Africa

Tsetse flies are vectors of African trypanosomes, protozoan parasites that cause sleeping sickness (or human African trypanosomosis) in humans and nagana (or animal African trypanosomosis) in livestock. In add...

Authors: Gisele M. S. Ouedraogo, Güler Demirbas-Uzel, Jean-Baptiste Rayaisse, Geoffrey Gimonneau, Astan C. Traore, Antonios Avgoustinos, Andrew G. Parker, Issa Sidibe, Anicet G. Ouedraogo, Amadou Traore, Bale Bayala, Marc J. B. Vreysen, Kostas Bourtzis and Adly m. M. Abd-Alla

Analysis of the gut-specific microbiome from field-captured tsetse flies, and its potential relevance to host trypanosome vector competence

The tsetse fly (Glossina sp.) midgut is colonized by maternally transmitted and environmentally acquired bacteria. Additionally, the midgut serves as a niche in which pathogenic African trypanosomes reside within...

Authors: Bridget C Griffith, Brian L Weiss, Emre Aksoy, Paul O Mireji, Joana E Auma, Florence N Wamwiri, Richard Echodu, Grace Murilla and Serap Aksoy

Coevolution of hytrosaviruses and host immune responses

Hytrosaviruses (SGHVs; Hytrosaviridae family) are double-stranded DNA (dsDNA) viruses that cause salivary gland hypertrophy (SGH) syndrome in flies. Two structurally and functionally distinct SGHVs are recognized...

Authors: Henry M. Kariithi, Drion G. Boucias, Edwin K. Murungi, Irene K. Meki, Güler Demirbaş-Uzel, Monique M. van Oers, Marc J. B. Vreysen, Adly M. M. Abd-Alla and Just M. Vlak

Enhancing vector refractoriness to trypanosome infection: achievements, challenges and perspectives

With the absence of effective prophylactic vaccines and drugs against African trypanosomosis, control of this group of zoonotic neglected tropical diseases depends the control of the tsetse fly vector. When ap...

Authors: Henry M Kariithi, Irene K Meki, Daniela I Schneider, Linda De Vooght, Fathiya M Khamis, Anne Geiger, Guler Demirbaş-Uzel, Just M Vlak, ikbal Agah iNCE, Sorge Kelm, Flobert Njiokou, Florence N Wamwiri, Imna I Malele, Brian L Weiss and Adly M M Abd-Alla

Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans

Tsetse flies (Diptera, Glossinidae) display unique reproductive biology traits. Females reproduce through adenotrophic viviparity, nourishing the growing larva into their modified uterus until parturition. Mal...

Authors: Francesca Scolari, Geoffrey Michael Attardo, Emre Aksoy, Brian Weiss, Grazia Savini, Peter Takac, Adly Abd-Alla, Andrew Gordon Parker, Serap Aksoy and Anna Rodolfa Malacrida

Diversity and phylogenetic relationships of Glossina populations in Nigeria and the Cameroonian border region

Tsetse flies are vectors of trypanosomes, parasites that cause devastating disease in humans and livestock. In the course of vector control programmes it is necessary to know about the Glossina species present in...

Authors: Stephen Saikiu Shaida, Judith Sophie Weber, Thaddeus Terlumun Gbem, Sen Claudine Henriette Ngomtcho, Usman Baba Musa, Mbunkha Daniel Achukwi, Mohammed Mamman, Iliya Shehu Ndams, Jonathan Andrew Nok and Soerge Kelm

Multi-drug resistant Enterobacter bugandensis species isolated from the International Space Station and comparative genomic analyses with human pathogenic strains

The antimicrobial resistance (AMR) phenotypic properties, multiple drug resistance (MDR) gene profiles, and genes related to potential virulence and pathogenic properties of five Enterobacter bugandensis strains ...

Authors: Nitin K. Singh, Daniela Bezdan, Aleksandra Checinska Sielaff, Kevin Wheeler, Christopher E. Mason and Kasthuri Venkateswaran

Prevalence of symbionts and trypanosome infections in tsetse flies of two villages of the “Faro and Déo” division of the Adamawa region of Cameroon

Tsetse flies are vectors of human and animal African trypanosomiasis. In spite of many decades of chemotherapy and vector control, the disease has not been eradicated. Other methods like the transformation of ...

Authors: Ginette Irma Kame-Ngasse, Flobert Njiokou, Tito Trésor Melachio-Tanekou, Oumarou Farikou, Gustave Simo and Anne Geiger

RNA interference-based antiviral immune response against the salivary gland hypertrophy virus in Glossina pallidipes

Glossina pallidipes salivary gland hypertrophy virus (GpSGHV; Hytrosaviridae) is a non-occluded dsDNA virus that specifically infects the adult stages of the hematophagous tsetse flies (Glossina species, Diptera:...

Authors: Irene K. Meki, Henry M. Kariithi, Andrew G. Parker, Marc J B Vreysen, Vera I D Ros, Just M Vlak, Monique M van Oers and Adly M. M. Abd-Alla

Different laboratory populations similar bacterial profile? The case of Glossina palpalis gambiensis

Microbiota plays an important role in the biology, ecology and evolution of insects including tsetse flies. The bacterial profile of 3 Glossina palpalis gambiensis laboratory colonies was examined using 16S rRNA ...

Authors: Vangelis Doudoumis, Antonios Augustinos, Aggeliki Saridaki, Andrew Parker, Adly M M Abd-Alla, Kostas Bourtzis and George Tsiamis

Combining paratransgenesis with SIT: impact of ionizing radiation on the DNA copy number of Sodalis glossinidius in tsetse flies

Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of the causative agents of African Trypanosomosis, which has been identified as a neglected tropical disease in both humans and animals in many regi...

Authors: Güler Demirbas-Uzel, Linda De Vooght, Andrew G. Parker, Marc J. B. Vreysen, Robert L. Mach, Jan Van Den Abbeele and Adly M. M. Abd-Alla

Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species

Tsetse flies (Diptera: Glossinidae) are solely responsible for the transmission of African trypanosomes, causative agents of sleeping sickness in humans and nagana in livestock. Due to the lack of efficient va...

Authors: Antonios A. Augustinos, Irene K. Meki, Guler Demirbas-Uzel, Gisele M. S. Ouédraogo, Aggeliki Saridaki, George Tsiamis, Andrew G. Parker, Adly M. M. Abd-Alla and Kostas Bourtzis

Impact of Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) on a heterologous tsetse fly host, Glossina fuscipes fuscipes

Tsetse flies (Diptera: Glossinidae) are the vectors of African trypanosomosis, the causal agent of sleeping sickness in humans and nagana in animals. Glossina fuscipes fuscipes is one of the most important tsetse...

Authors: Güler Demirbas-Uzel, Andrew G. Parker, Marc J. B. Vreysen, Robert L. Mach, Jeremy Bouyer, Peter Takac and Adly M. M. Abd-Alla

Towards improving tsetse fly paratransgenesis: stable colonization of Glossina morsitans morsitans with genetically modified Sodalis

Tsetse flies (Glossina sp.) refractory to trypanosome infection are currently being explored as potential tools to contribute in the control of human and animal African trypanosomiasis. One approach to disrupt tr...

Authors: Linda De Vooght, Severien Van Keer and Jan Van Den Abbeele

High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.)

In African tsetse flies Glossina, spp. detection of bacterial symbionts such as Wolbachia is challenging since their prevalence and distribution are patchy, and natural symbiont titers can range at levels far bel...

Authors: Daniela I Schneider, Andrew G Parker, Adly M Abd-alla and Wolfgang J Miller

Metarhizium anisopliae infection reduces Trypanosoma congolense reproduction in Glossina fuscipes fuscipes and its ability to acquire or transmit the parasite

Tsetse fly-borne trypanosomiasis remains a significant problem in Africa despite years of interventions and research. The need for new strategies to control and possibly eliminate trypanosomiasis cannot be ove...

Authors: Lawrence G Wamiti, Fathiya M Khamis, Adly M M Abd-alla, Fidelis L O Ombura, Komivi S Akutse, Sevgan Subramanian, Samuel O Odiwuor, Shem J Ochieng, Sunday Ekesi and Nguya K Maniania

Special issue on enhancing vector refractoriness to trypanosome infection-foreword

Authors: Adly M. M. Abd-Alla, George Tsiamis and Drion G. Boucias

Dialogue between Staphylococcus aureus SA15 and Lactococcus garvieae strains experiencing oxidative stress

Staphylococcus aureus is an important foodborne pathogen. Lactococcus garvieae is a lactic acid bacterium found in dairy products; some of its strains are able to inhibit S. aureus growth by producing H₂O₂. Three...

Authors: Clothilde Queiroux, Muriel Bonnet, Taous Saraoui, Pierre Delpech, Philippe Veisseire, Etienne Rifa, Cécile Moussard, Geneviève Gagne, Céline Delbès and Stéphanie Bornes

Screening, purification and characterization of thermostable, protease resistant Bacteriocin active against methicillin resistant Staphylococcus aureus (MRSA)

The emergence of serious issues of multidrug resistance in the past few years have enforced the use of bacteriocins for combating infections. Threat posed to public health by various multidrug resistant (MDR) ...

Authors: Asma Ansari, Rashida Rahmat Zohra, Omer Mukhtar Tarar, Shah Ali Ul Qader and Afsheen Aman

A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

Several fungi-specific primers target the 18S rRNA gene sequence, one of the prominent markers for fungal classification. The design of most primers goes back to the last decades. Since then, the number of seq...

Authors: Stefanos Banos, Guillaume Lentendu, Anna Kopf, Tesfaye Wubet, Frank Oliver Glöckner and Marlis Reich

Prediction of pathogenesis-related secreted proteins from Stemphylium lycopersici

Gray leaf spot is a devastating disease caused by Stemphylium lycopersici that threatens tomato-growing areas worldwide. Typically, many pathogenesis-related and unrelated secreted proteins can be predicted in ge...

Authors: Rong Zeng, Shigang Gao, Lihui Xu, Xin Liu and Fuming Dai

Metagenomics of pasteurized and unpasteurized gouda cheese using targeted 16S rDNA sequencing

The microbiome of cheese is diverse, even within a variety. The metagenomics of cheese is dependent on a vast array of biotic and abiotic factors. Biotic factors include the population of microbiota and their ...

Authors: Joelle K. Salazar, Christina K. Carstens, Padmini Ramachandran, Arlette G. Shazer, Sartaj S. Narula, Elizabeth Reed, Andrea Ottesen and Kristin M. Schill

Taxonomic classification for microbiome analysis, which correlates well with the metabolite milieu of the gut

16S rRNA gene amplicon sequencing analysis (16S amplicon sequencing) has provided considerable information regarding the ecology of the intestinal microbiome. Recently, metabolomics has been used for investiga...

Authors: Yoshihisa Wakita, Yumi Shimomura, Yusuke Kitada, Hiroyuki Yamamoto, Yoshiaki Ohashi and Mitsuharu Matsumoto

Multi-drug resistant Salmonella enterica serovar Typhi isolates with reduced susceptibility to ciprofloxacin in Kenya

Typhoid fever remains a public health concern in developing countries especially among the poor who live in informal settlements devoid of proper sanitation and clean water supply. In addition antimicrobial re...

Authors: Winnie C. Mutai, Anne W. T. Muigai, Peter Waiyaki and Samuel Kariuki

Characteristics of NDM-1-producing Klebsiella pneumoniae ST234 and ST1412 isolates spread in a neonatal unit

The emergence of carbapenem-resistant Klebsiella pneumoniae (CR-KP) has become a significant problem worldwide and also being a major threat to children and newborns. Here we report an outbreak of NDM-1-producing...

Authors: Xu Huang, Xiangjun Cheng, Pengfei Sun, Chenjie Tang, Fang Ni and Genyan Liu

MALDI-TOF mass spectrometry-based serotyping of V. parahaemolyticus isolated from the Zhejiang province of China

Vibrio parahaemolyticus is as an important food-borne pathogen circulating in China. Since 1996, the core serotype has become O3:K6, which has specific genetic markers. This serotype causes the majority of outbre...

Authors: Ping Li, Wenwen Xin, Susu Xia, Yun Luo, Zhongwen Chen, Dazhi Jin, Shan Gao, Hao Yang, Bin Ji, Henghui Wang, Yong Yan, Lin Kang and Jinglin Wang