Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenase
BMC Microbiology volume 17, Article number: 154 (2017)
Bacillus subtilis is able to utilize at least three inositol stereoisomers as carbon sources, myo-, scyllo-, and D-chiro-inositol (MI, SI, and DCI, respectively). NAD+-dependent SI dehydrogenase responsible for SI catabolism is encoded by iolX. Even in the absence of functional iolX, the presence of SI or MI in the growth medium was found to induce the transcription of iolX through an unknown mechanism.
Immediately upstream of iolX, there is an operon that encodes two genes, yisR and iolQ (formerly known as degA), each of which could encode a transcriptional regulator. Here we performed an inactivation analysis of yisR and iolQ and found that iolQ encodes a repressor of the iolX transcription. The coding sequence of iolQ was expressed in Escherichia coli and the gene product was purified as a His-tagged fusion protein, which bound to two sites within the iolX promoter region in vitro.
IolQ is a transcriptional repressor of iolX. Genetic evidences allowed us to speculate that SI and MI might possibly be the intracellular inducers, however they failed to antagonize DNA binding of IolQ in in vitro experiments.
Epimerization of the hydroxyl groups of cyclohexane 1,2,3,4,5,6-hexol (inositol) generates nine different stereoisomers. The most abundant form in nature is cis-1,2,3,5-trans-4,6-cyclohexanehexol (myo-inositol, MI) (Fig. 1), which is an essential component of phosphatidylinositol in the cell membranes of eukaryotes and exists as myo-inositol hexakisphosphate (phytic acid) in plant seeds . Other inositol stereoisomers occur rarely in nature, although some exert specific and physiologically important effects. For example, D-chiro-inositol (DCI) (Fig. 1) and its 3-O-methyl derivative, D-pinitol, are beneficial for patients with hyperglycemia or polycystic ovary syndrome [2, 3], and scyllo-inositol (SI) (Fig. 1) directly interacts with beta-amyloid peptides to inhibit their aggregation in the brain and block the development of Alzheimer disease .
Bacillus subtilis efficiently utilizes inositol stereoisomers such as MI, DCI, and SI as carbon sources . The iolABCDEFGHIJ operon encodes the enzymes that catabolize MI and DCI (Fig. 1). Two inositol transporters are encoded by iolF and iolT for MI and SI uptake [6, 7]. MI dehydrogenase, encoded by iolG, converts MI to scyllo-inosose (SIS) and reduces NAD+ in the first reaction of the catabolic pathway . IolG reacts on both MI and DCI but not on SI . The iol operon and iolT are regulated by the IolR transcriptional repressor, which is antagonized by the product of IolC kinase, 2-deoxy-5-keto-gluconic acid-6-phosphate [6, 10, 11]. On the other hand, the inositol dehydrogenases IolX and IolW are specific for SI and require NAD+ and NADP+, respectively . Each enzyme converts SI to SIS, which is the same product generated from MI by IolG. Recently, IolU was found as the third SI dehydrogenase, which only can reduce SIS into SI in an NADPH-dependent manner . Transcription of iolX is induced by the addition of SI to the growth medium as the sole carbon source . Transcription of iolW is constitutive but it does not contribute to growth on SI, suggesting that IolX is essential for the catabolism of SI and that IolW is required for other reactions such as the generation of SI from SIS [5, 7].
The mechanism underlying the regulation of iolX to degrade SI is unknown. Within the B. subtilis genome, yisR and iolQ (formerly known by degA) reside upstream of iolX and are predicted to encode transcriptional regulators that belong to the AraC/XylS and LacI families, respectively (Fig. 1). Members of the AraC/XylS family include a positive regulator such as AdaA that induce the alkA and ada operons in B. subtilis . In contrast, most members of the LacI family are negative regulators, such as CcpB , KdgR , ExuR , and LacR  in B. subtilis. A transcriptome analysis revealed that yisR and iolQ were transcribed from a single operon . The function of YisR is unknown and its regulatory function has never been studied. On the other hand, IolQ (DegA) was named after the discovery that the recombinant form produced in Escherichia coli accelerated the degradation of glutamine phosphoribosyl pyrophosphate amidotransferase, implying that it might be a protease . However, its sequence similarities to regulatory proteins CytR, LacI, GalR, and PurR of E. coli and CcpA of B. subtilis suggest that it could have stimulated the production of a protease . In the present study, we therefore investigated the possible involvement of YisR and IolQ in the regulation of iolX. We show that iolQ encodes a transcriptional repressor that binds to the promoter region of iolX.
Bacterial strains, plasmid and growth conditions
The bacterial strains and plasmids used in this study are listed in Table 1. B. subtilis strain 168 is our standard strain for the study of inositol catabolism. The mutant strain BFS3018 was constructed from strain 168 and acquired from the National Bio Resource Project, National Institute of Genetics, Japan. BFS3018 has a pMUTIN4 (lacZ lacI amp erm)  integration to disrupt iolX which allows us to monitor iolX expression in an iolX mutated context by β-galactosidase activity . The other B. subtilis mutant strains were constructed as described below. E. coli strains DH5α (Sambrook & Russell, 2001) and BL21 (DE3) (Merck Millipore) served as hosts for plasmid construction and expression of C-terminal His6-tagged proteins, respectively.
E. coli strains were maintained in lysogeny broth (LB) medium and B. subtilis strains were maintained using a tryptose blood agar base (Becton Dickinson) or S6 liquid medium  containing 0.5% casamino acid (Becton Dickinson) and 0.005% L-tryptophan. Plasmids pMD20 (Takara Bio) and pET30a (Merck Millipore) served as vectors for TA-cloning and His6-tag construction, respectively. Antibiotics used as required were as follows: erythromycin (0.5 μg ml−1), ampicillin (50 μg ml−1), and kanamycin (50 μg ml−1). Media were supplemented with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) or 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) as required. All bacteria were cultured at 37 °C with rotary shaking at 150 rpm.
Construction of B. subtilis mutants
CM101 (ΔyisR) and CM102 (ΔiolQ) were constructed using the marker-free approach of Morimoto et al. . The pop-in construction was made by ligation of three different polymerase chain reaction (PCR) fragments amplified from the 168 genome (Fig. 2a) and another one comprising the mazF cassette . The fragments were i) the first PCR fragment for region A located upstream of the deletion target, ii) the second for region B located downstream of the target, iii) the third for region C located inside the target, iv) and the mazF cassette constituted of mazF for suicidal toxin under the control of IPTG-inducible promoter (Pspac), lacI for Lac repressor controlling Pspac, and the spectinomycin resistance gene (spc). For the construction of CM101, the PCR fragments of regions A, B, C, and the mazF cassette were amplified using the primer pairs DyisRAF/DyisRAR, DyisRBF/DyisRBR, DyisRCF/DyisRCR, and MazFfw/MazFbw, respectively (Table 2). For CM102, the PCR fragments of regions A, B, C, and the mazF cassette were amplified using the primer pairs DdegAAF/DiolQAR, DiolQBF/DiolQBR, DiolQCF/DiolQCR, and MazFfw/MazFbw, respectively (Table 2). The pop-in construction containing the regions A, B, the mazF cassette, and region C in that order (Fig. 2b) was used to transform the parental strain 168 of B. subtilis for spectinomycin resistance via a double crossover in the homologous regions A and C, introducing the mazF cassette into the targeted region (Fig. 2c). The spectinomycin-resistant transformants were then screened on IPTG-containing plates for the detection of spectinomycin sensitive mutants. In such mutants, an intrachromosomal crossover event between the two direct repeat stretches corresponding to region B occurred to eliminate the mazF cassette and resulted in the marker-free deletion of the stretch between regions A and B (Fig. 2d). Correct construction of strains CM101 and CM102 was confirmed by sequencing (data not shown).
NAD+-dependent SI dehydrogenase activities in cell extracts were measured spectrophotometrically with an increase in absorbance at 340 nm with the generation of NADH as previously described . β-Galactosidase activities in cell extracts were determined as previously described .
B. subtilis strains were grown at 37 °C with shaking in S6 medium containing 0.5% casamino acid, 0.005% L-tryptophan (Becton Dickinson) with or without MI or SI (10 mM each), and 10 mM glucose was added as required. Total RNAs were extracted from the cells and purified as previously described .
The RNA samples were subjected to a Northern blot analysis using a DIG-labeled RNA probe specific for iolX. The RNA probe was prepared as follows: A DNA fragment corresponding to part of the iolX-coding region was PCR-amplified using strain 168 DNA as a template and the primers NiolX and NiolXDIG (Table 2) to introduce a T7 RNA polymerase promoter sequence at their 3′-termini. The PCR product was used as the template for in vitro transcription using a DIG RNA labeling kit (SP6/T7) (Roche Diagnostics, Basel, Switzerland) to produce the DIG-labeled RNA probe. Cellular RNAs were separated using gel electrophoresis, transferred to a positively charged nylon membrane (Roche Diagnostics), and hybridized using the DIG-labeled probe according to the manufacturer’s instructions. Hybrids were detected using a DIG luminescence detection kit (Roche Diagnostics).
Primer extension was performed to identify the transcriptional start site of the iolX transcript . Reverse transcription initiated from the PiolX400-R primer (Table 2) was labeled at the 5′-terminus using a Megalabel kit (Takara Bio) and [γ-32P]ATP (PerkinElmer). DNA from strain 168 used as the template for the dideoxy sequencing reactions, which initiated from the same end-labeled primer used for ladder preparation, was prepared by PCR using the primers PiolX400-F/PiolX400-R (Table 2).
DNA fragments corresponding to the coding regions of iolQ and yisR were amplified from B. subtilis 168 genomic DNA by PCR using the respective primers iolQNdeI-F/iolQXhoI-R and yisRNdeI-F/yisRXhoI-R with generation of NdeI and XhoI sites at the 5′- and 3′-termini of each amplicon, respectively (Table 2). Each PCR product was ligated to the arms of pMD20 (Takara Bio) using a Mighty TA-cloning kit (Takara Bio) and was used to transform E. coli DH5α, which was then cultured on LB plates containing ampicillin, IPTG, and X-gal. White colonies were selected and plasmid DNAs were subjected to a sequence analysis using an ABI PRISM 3100 Genetic Analyzer (Thermo Fisher Scientific). The recombinant plasmids with the correct sequences were digested using NdeI and XhoI, and the restriction fragments were ligated to the arms of NdeI/XhoI-cleaved pET-30a to generate pET-iolQ or pET-yisR, which were used to transform E. coli BL21 (DE3) to produce C-terminal His6-tagged proteins IolQ-His6 and YisR-His6, respectively.
Protein production and purification
E. coli BL21 (DE3) transformed with pET-iolQ or pET-yisR was inoculated into LB medium containing kanamycin and cultured at 37 °C with shaking. The recombinant proteins were induced using 1 mM IPTG when the optical density of the culture reached OD660 = 0.35, and the culture was further incubated for 2 h at 37 °C with shaking; the cells were harvested and disrupted by sonication. IolQ-His6 and YisR-His6 were purified from cell lysates using a TALON metal-affinity resin (Takara Bio) according to the manufacturer’s instructions.
Gel mobility shift assay
Gel mobility shift assays were performed according to a previous study . DNA fragments of the 200-bp sequences of the iolX and yisR-iolQ promoter regions were PCR-amplified using the specific primers iolX (−200)-F/iolX (−1)-R and yisR (−200)-F/yisR (−1)-R, respectively (Table 2). A negative control of a 100 bp fragment representing a segment of the iolW coding region was amplified using the primers GMSA-Nega-F/GMSA-Nega-R (Table 2). Each DNA fragment (0.155 pmol) was incubated in 0.02 ml of binding buffer [10 mM Tris-HCl (pH 8.0), 1 mM DTT, 10 mM KCl, 5 mM MgCl2, 10% glycerol, 5 μg ml−1 poly d(I-C), and 50 μg ml−1 bovine serum albumin] at 37 °C for 30 min with varying amounts of IolQ-His6 or YisR-His6. DNA protein complexes were separated using nondenaturing polyacrylamide gels in TAE buffer. The DNA fragments in the gel were stained using SYBR Green for 30 min and the bands were visualized using Chemi Doc XRS+ with Image Lab software (Bio-Rad).
DNase I footprint assay
PCR reactions were used to amplify 5′-6-[FAM]-labeled DNA fragments containing the iolX promoter region (300 bp) from the DNA of strain 168 using the specific primers [FAM]iolX(−250)-F/iolX (+50)-R and iolX (−250)-F/[FAM]iolX(+50)-R for labeling the sense and antisense strands, respectively (Table 2). Each differentially 5′-6-[FAM]-labeled DNA fragment (0.45 pmol) was incubated in 0.2 ml of binding buffer with varying amounts of IolQ-His6 at 37 °C for 30 min. 0.75 units of DNase I (Takara Bio) was added to digest the DNA for 5 min, and the reaction was stopped by adding 0.2 ml of 0. 5 M EDTA. DNAs were extracted using a PCR purification kit (Promega). DNA sequencing of the sense and antisense strands employed the primers iolX (−250)-F and iolX (+50)-R, respectively, using the Thermo Sequenase Dye Primer Manual Cycle Sequence Kit (USB). The DNA samples were analyzed by Sigma-Aldrich using an ABI 3130xl Genetic Analyzer and ABI Gene Mapper Software Ver. 4.0 (Thermo Fisher Scientific).
SI and MI induce the transcription of iolX
As shown in Fig. 3a, in the standard strain 168, NAD+-dependent SI dehydrogenase activity was induced in the presence of SI up to 40-fold more than its absence, while it completely disappeared in strain BSF3018 with the inactivation of iolX through pMUTIN4 integration (Fig. 3b). It was previously reported that BSF3018 did not grow when depending on SI as the sole carbon source . In B. subtilis, there are at least two NADP+-dependent SI dehydrogenases, IolW and IolU, however neither of them functions to dehydrogenate SI to degrade it as the carbon source [12, 13]. Therefore, SI induced iolX to produce NAD+-dependent SI dehydrogenase that was responsible for the physiological utilization of SI in B. subtilis. Although iolX does not play a role in the MI catabolism , MI was also able to induce NAD+-dependent SI dehydrogenase activity up to 20-fold more than in its absence, indicating that MI also could induce iolX (Fig. 3a).
On the other hand, in strain BFS3018, iolX was inactivated but its transcription was monitored by the expression of lacZ for β-galactosidase activity instead (Fig. 3b). As shown in Fig. 3c, in the presence of SI and MI, β-galactosidase activity was induced up to 50- and 10-fold more than in their absence, respectively, indicating that both SI and MI are able to induce iolX at the transcription level without functional iolX. As shown in Fig. 1, SI and MI are degraded to produce the same set of intermediates [11, 12], and we can consider that none of them could be made from SI when iolX was inactivated, as BSF3018 did not grow when depending on SI as the sole carbon source . Consequently, it is unlikely that any of the intermediates were involved in the transcriptional induction of iolX.
We previously reported that not only MI but also SI was mainly imported by the IolT transporter . As the expression of iolT is controlled by IolR , it is thus induced when MI or SI is degraded down to the product of the IolC reaction (Fig. 1), 2-deoxy-5-keto-gluconic acid-6-phosphate, which antagonizes DNA binding of IolR . Since SI can never be converted into the IolC-reaction product in BFS3018 due to the inactivation of iolX, the results suggest that SI uptake supported by the basal expression of iolT could be enough to allow induction of iolX. On the other hand, in BFS3018, MI is degraded involving IolG, thus allowing the induction of iolT. Therefore, the induction of β-galactosidase activity of BFS3018 in response to MI could be achieved due to the elevated levels of MI uptake. Nevertheless, the activity was still less than that produced in response to SI.
As shown in Fig. 4, the Northern blot analysis confirmed that the transcription of iolX in strain 168 was induced in the presence of SI or MI. The induction of NAD+-dependent SI dehydrogenase activity in strain 168 in the presence of SI or MI was abolished by additional glucose, suggesting that iolX could be under catabolite repression (Fig. 3a). In addition, the induction of β-galactosidase activity of BFS3018 in response to SI and MI was also abolished by additional glucose. These results indicatied that the induction and catabolite repression of iolX occurred at the transcription level (Fig. 3c).
Expression of iolQ is required to regulate iolX transcription in response to SI
Immediately upstream of iolX, there is an operon that encodes two genes, yisR and iolQ , each of which could encode a transcriptional regulator; yisR and iolQ were predicted to encode transcriptional regulators that belong to the AraC/XylS and LacI families, respectively (Fig. 1). To determine whether YisR and IolQ regulate iolX, we generated the mutant strains CM101 and CM102 (Fig. 3a). In CM101 (ΔyisR), yisR was deleted to avoid the polar effect on iolQ downstream of it, while in CM102 (ΔiolQ), iolQ was alternatively deleted. Therefore, only iolQ was expressed under the control of the original yisR-iolQ promoter in CM101 whereas only yisR was expressed in CM102.
In CM101 (ΔyisR), the NAD+-dependent SI dehydrogenase activity of IolX was repressed in the absence of SI or MI and induced in their presence, while in CM102 (ΔiolQ) it became constitutive to be almost 50-fold higher than that in strain 168 in the absence of SI or MI (Fig. 3a). The activities in CM101 and CM102 in the presence of SI and MI seemed higher than those in strain 168 by unknown reasons. On the other hand, the activities in both CM101 and CM102 were repressed in the presence of glucose. These results suggest that induction of iolX could be regulated by IolQ but not by YisR. In addition, neither IolQ nor YisR could be involved in the catabolite repression of iolX.
The Northern blot analyses revealed that, in CM102 without functional iolQ, iolX was transcribed in the absence of SI and MI (Fig. 4). However, the transcription was shut off in CM101 (ΔyisR) when SI and MI were absent, and it was obviously induced in response to SI and MI. These results indicate that the transcriptional regulation of iolX in response to SI and MI depended on iolQ but not on yisR.
IolQ binds to the iolX promoter region
IolQ-His6 and YisR-His6 (Fig. 5) were tested for their binding to DNA fragments containing either promoter region of the iolX or yisR-iolQ operon. Gel mobility shift assays revealed that IolQ-His6 formed complexes with the DNA fragment of the iolX promoter region (Fig. 5). The IolQ-DNA complexes formed distinct two bands, the lower and the higher molecular weight bands. As the concentrations of IolQ-His6 were elevated, the former appeared first at the lower concentrations, which shifted to form the latter exclusively as the concentrations increased further (Fig. 5). The results indicate that the iolX promoter fragment may contain at least two IolQ-binding sites with different affinities (Fig. 5); the lower molecular weight band could correspond to the IolQ-DNA complex formed by IolQ binding only to a higher affinity site while the higher molecular weight one was formed by its binding to both higher and lower affinity sites. Neither SI nor MI (at higher concentrations up to 20 mM) affected the specific DNA binding of IolQ-His6 in vitro (data not shown). In addition, another set of gel mobility shift experiments involving not only IolQ-His6 but also YisR-His6 was conducted. Nevertheless, neither SI, MI, nor SIS caused any effect on DNA binding of IolQ-His6 in the additional presence of YisR-His6 (data not shown).
On the other hand, IolQ did not interact with the yisR-iolQ promoter region, and we failed to detect YisR-His6 binding to either fragment of the iolX or yisR-iolQ promoter region in the presence and absence of any of MI, SI, and SIS (data not shown).
Identification of the two IolQ-binding sites within the iolX promoter region
The primer extension experiment (Fig. 6) determined two transcriptional start sites downstream of the promoters P1 and P2 for the iolX transcript. Only a small amount of the reverse transcript corresponding to promoter P1 was detected in the absence of SI, but it was significantly induced in response to SI together with the additional transcript corresponding to P2. Their respective −35 and −10 regions were deduced to serve as the iolX promoters P1 and P2 (Fig. 7). Another reverse transcript was found to be as strong as the one corresponding to promoter P1 but was shorter by 6 bp. This was considered to be due to a truncated product derived from the P1 transcript, since there are no consensus −35 and −10 sequences corresponding to this 5′ end.
IolQ-binding sites within the iolX promoter region identified using a DNase I footprint analysis revealed that IolQ bound with different affinities to the two regions (Fig. 7). The stretches with sequences TCTTTTGAGAAAGCGCTTGCGCAAAAT (spanning +4 to +30 bp, position numbers assigned relative to the transcription start site of the promoter P2) and AGAGAAAACGCTTTCTCAAAG (spanning +68 to +88 bp) were protected from DNase I at lower and higher concentrations of IolQ, respectively (Fig. 7). Therefore, the former and the latter stretches were judged as the higher and lower affinity regions, respectively. The two protected regions contained the conserved sequence AGAAARCGCTTKCKCAAA (where R = A or G and K = G or T), which may represent a core recognition sequence required for IolQ binding. The protected stretch of the higher affinity region extended 7 bp upstream and 1 bp downstream compared with that of the lower affinity site. Previously, a plausible cre site for CcpA/P-Ser-HPr binding was predicted in the iolX promoter region , which was found to be overlapping the lower affinity region and was supposed to be involved in catabolite repression (Fig. 7). In addition, we could also predict another plausible cre site within the higher affinity region.
B. subtilis strains possess at least three types of SI dehydrogenases encoded by iolX, iolW , and iolU . IolX requires NAD+ and both IolW and IolU need NADP+ as a cofactor. It is known that iolX plays an indispensable role in the utilization of SI as a carbon source for growth , and we showed here that iolX was induced more than 40-fold in the presence of SI (Figs. 3 and 4). The transcription of iolW is constitutive, and IolW can convert SI into SIS in vitro but does not contribute to growth depending on the availability of SI as the carbon source . IolU is also produced constitutively and generally at low levels  and was not able to dehydrogenate SI but only reduce SIS into SI . We hypothesized that yisR and iolQ, which are located and cotranscribed  immediately upstream of iolX, might encode the regulator(s) of iolX transcription (Fig. 1). YisR is a member of the AraC/XylS family, which includes mainly positive transcription regulators , and IolQ is a member of the LacI family of negative transcription regulators , which contain the typical helix-turn-helix motif, characteristic of a DNA-binding domain . The present results suggested that YisR was unlikely to be involved in the regulation of iolX transcription (Figs. 3 and 4). Usually, the regulatory function of AraC/XylS family members requires specific cofactors; for example, B. subtilis Btr needs binding with its co-activator, the siderophore bacillibactin, to exert its regulatory function . Therefore, we hypothesized that one of MI, SI, and SIS might be a cofactor of YisR, but none of them enhanced YisR-His6 binding to the iolX and yisR-iolQ promoter regions. On the other hand, since the DNA binding motif of AraC family proteins is near the C-terminus, the C-terminal His-tag fusion of YisR-His6 could affect DNA binding. Obviously, further studies are required to clarify transcriptional regulation involving YisR.
The data presented here indicate that iolQ encodes a repressor that binds to two sites within the iolX promoter region (Figs. 5 and 7). In addition, the repression is released in the presence of SI or MI (Figs. 3 and 4). iolX encodes NAD+-dependent SI dehydrogenase that is responsible for physiological SI catabolism . Even when we functionally inactivated iolX in BF3018 by inserting pMUTIN4, the transcription of iolX-lacZ was prominently elevated in media containing SI (Fig. 3c). We considered the possibility that the inducing signal was a derivative of SI not requiring IolX for its synthesis. However, we failed to identify any good candidates. Although IolW is constitutively produced, it only inefficiently coverts SI into SIS with the predominating reverse reaction . We previously demonstrated that MI was converted into SI through the coupling reactions involving IolG and IolW; the former dehydrogenates MI into SIS with a reduction of NAD+, and the latter reduces SIS into SI with oxidation of NADPH . However, the conversion was detected only when the intermediate SIS was accumulated by the additional inactivation of iolE, which encodes SIS dehydratase acting on SIS for further degradation of this intermediate (Fig. 1) . Another NADP+-dependent SI dehydrogenase encoded by iolU was recently identified . Although this enzyme is not as active as IolW, it is able to convert SIS into SI but only when overexpressed. Therefore, IolU is unlikely to be involved in the possible conversion of MI into SI. All of these observations led us to speculate that mainly SI and secondarily MI could be the intracellular inducers interacting with IolQ to antagonize its DNA binding, allowing the induction of iolX, however they failed to antagonize DNA binding of IolQ-His6 in vitro. The C-terminal His-tag fusion might affect effector binding.
We showed here that IolQ bound with different affinities to the two sites within the iolX promoter region. The high affinity site was located from positions +4 to +30 of the promoter P2 within the sequence TCTTTTGAGAAAGCGCTTGCGCAAAAT, and the low affinity site was located from +68 to +88 within the sequence AGAGAAAACGCTTTCTCAAAG (Fig. 7). Most members of the LacI family preferentially require a palindromic sequence within their DNA binding sites . A comparison between the sequences of the two IolQ binding sites identified the relatively conserved sequence AGAAARCGCTTKCK, which may suggest the potential perfect palindrome could be AGAAAGCGCTTTCT. However, this perfect palindrome is not present in either of the two binding sites that differ in two and one positions in the higher and lower affinity binding sites, respectively. Therefore, the consensus palindrome is not the only determinant of IolQ binding, although the sequences extending from the conserved stretch may contribute to high affinity binding of IolQ to its target sequence. Within the B. subtilis genome, there are 22 sites with a sequence similar to the conserved consensus sequence (maximum of two different positions, data not shown). At least seven of the 22 sites are located close to promoter regions, including the one of the iolX promoter. Thus, IolQ may regulate six additional promoters and therefore drive the transcription of at least the following genes (products): glpT (glycerol-3-phosphate permease), ycsA (putative enzyme similar to 3-isopropylmalate dehydrogenase), acoR (transcriptional activator of acetoin utilization genes), yrbE (another member of the Gfo/Idh/MocA family paralogs including iolG, iolU, iolW, and iolX) , menA (1,4-dihydroxy-2-naphthoate octaprenyltransferase), and bglS (endo-β-1,3–1,4 glucanase). Our future course will focus on determining the mechanisms of transcriptional regulation of these genes and their involvement in SI metabolism.
Expression of iolX for NAD+-dependent SI dehydrogenase activity in strain 168 as well as the β-galactosidase activity in strain BFS3018 was almost completely repressed in response to glucose even in the presence of SI and MI, indicating that iolX is under catabolite repression (Fig. 3a and c). The plausible cre site predicted as overlapping the lower affinity region for IolQ binding (Fig. 7) might be involved in catabolite repression. We noticed that part of the conserved sequence AGAAARCGCTTKCK for IolQ binding was quite similar to the one WGNAANCGNTTNCW for CcpA/P-Ser-HPr biding . In addition, the sequence AGAAAGCGCTTGCGC within the higher affinity site for IolQ binding was also similar to the cre site consensus (Fig. 7). Both or either of the two IolQ-binding sites might also function as the binding site of CcpA/P-Ser-HPr in the presence of glucose. Since iolX functions for the catabolism of SI as a minor alternative carbon source, it makes sense that this gene is regulated by global catabolite repression involving CcpA/P-Ser-HPr .
In B. subtilis, both SI and MI induce iolX expression for NAD+-dependent SI dehydrogenase activity. The iolX expression became constitutive in an iolQ background, and IolQ binds to two sites upstream of iolX where two transcription start sites were located. Genetic evidences allowed us to speculate that SI and MI might possibly be the intracellular inducers; however they failed to antagonize DNA binding of IolQ in in vitro experiments.
polymerase chain reaction
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Irvine RF, Schell MJ. Back in the water: the return of the inositol phosphates. Nat Rev Mol Cell Biol. 2001;2:327–38.
Larner J. D-chiro-inositol – its functional role in insulin action and its deficit in insulin resistance. Int J Exp Diabetes Res. 2002;3:47–60.
Iuorno M, Jakubowicz D, Baillargeon J, Dillon P, Gunn R, et al. Effects of D-chiro-inositol in lean women with the polycystic ovary syndrom. Endocr Pract. 2002;8:417–23.
McLaurin J, Golomb R, Jurewicz A, Antel JP, Fraser PE. Inositol stereoisomers stabilize an oligomeric aggregate of Alzheimer amyloid beta peptide and inhibit Abeta-induced toxicity. J Biol Chem. 2000;275:18495–502.
Yamaoka M, Osawa S, Morinaga T, Takenaka S, Yoshida K. A cell factory of Bacillus subtilis engineered for the simple bioconversion of myo-inositol to scyllo-inositol, a potential therapeutic agent for Alzheimer’s disease. Microb Cell Factories. 2011;10:69.
Yoshida K, Yamamoto Y, Omae K, Yamamoto M, Fujita Y. Identification of two myo-inositol transporter genes of Bacillus subtilis. J Bacteriol. 2002;184:983–91.
Morinaga T, Matsuse T, Ashida H, Yoshida K. Differential substrate specificity of two inositol transporters of Bacillus subtilis. Biosci Biotechnol Biochem. 2010;74:1312–4.
Yoshida K, Aoyama D, Ishio I, Shibayama T, Fujita Y. Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis. J Bacteriol. 1997;179:4591–8.
Ramaley R, Fujita Y, Freese E. Purification and properties of Bacillus subtilis inositol dehydrogenase. J Biol Chem. 1979;254:7684–90.
Yoshida K, Shibayama T, Aoyama D, Fujita Y. Interaction of a repressor and its binding sites for regulation of the Bacillus subtilis iol divergon. J Mol Biol. 1999;285:917–29.
Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, et al. myo-inositol catabolism in Bacillus subtilis. J Biol Chem. 2008;283:10415–24.
Morinaga T, Ashida H, Yoshida K. Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis. Microbiology. 2010;156:1538–46.
Kang DM, Tanaka K, Takenaka S, Ishikawa S, Yoshida K. Bacillus subtilis iolU encodes an additional NADP+-dependent scyllo-inositol dehydrogenase. Biosci Biotechnol Biochem. 2017;81:1026–32.
Morohoshi F, Hayashi K, Munkata N. bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon. J Bacteriol. 1993;175:6010–7.
Chauvaux S, Paulsen IT, Saier MH. CcpB, a novel transcription factor implicated in catabolite repression in Bacillus subtilis. J Bacteriol. 1998;180:491–7.
Lin JS, Shaw GC. Regulation of the kdulD operon of Bacillus subtilis by the KdgR repressor and the ccpA gene: identification of two KdgR-binding sites within the kdgR-kdul intergenic region. Microbiology. 2007;153:701–10.
Mekjian KR, Bryan EM, Beall BW, Moran CP. Regulation of hexuronate utilization in Bacillus subtilis. J Bacteriol. 1999;181:426–33.
Daniel RA, Haiech J, Denizot F, Errington J. Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR. J Bacteriol. 1997;179:5636–8.
Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, et al. Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science. 2012;335:1103–6.
Bussey LB, Switzer RL. The degA gene product accelerates degradation of Bacillus subtilis phosphoribosylpyrophosphate amidotransferase in Escherichia coli. J Bacteriol. 1993;175:6348–53.
Vagner V, Dervyn E, Ehrlich SD. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology. 1998;144:3097–104.
Fujita Y, Freese E. Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase. J Bacteriol. 1981;145:760–7.
Morimoto T, Ara K, Ozaki K, Ogasawara N. A simple method for introducing marker-free deletions in the Bacillus subtilis genome. Methods Mol Biol. 2011;765:345–58.
Sambrook J, Russell DW. Molecular cloning: a laboratory manual. 3rd ed. N.Y: Cold Spring Harb Lab Press. Cold spring Harbor; 2001.
Yoshida K, Ishio I, Nagakawa E, Yamamoto Y, Yamamoto M, Fujita Y. Systematic study of gene expression and transcription organization in the gnt-ywaA region of the Bacillus subtilis genome. Microbiology. 2000;146:573–9.
Meyer U, Rensing L. A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE)-binding activity in Neurospora crassa. Fungal Genet Newsl. 2009;45:19–21.
Marciniak BC, Pabijaniak M, de Jong A, Dűhring R, Seidel G, et al. High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis. BMC Genomics. 2012;13:401.
Fukami-Kobayashi K. Parallel evolution of ligand specificity between LacI/GalR family repressors and periplasmic sugar-binding proteins. Mol Biol Evol. 2003;20:267–77.
Weickert MJ, Adhya S. A family of bacterial regulators homologous to gal and lac repressors. J Biol Chem. 1992;267:15869–74.
Gaballa A, MacLellan S, Helmann JD. Transcription activation by the siderophore sensor Btr is mediated by ligand-dependent stimulation of promoter clearance. Nucleic Acids Res. 2012;40:3585–95.
Fujita Y. Carbon catabolite control of the metabolic network in Bacillus subtilis. Biosci Biotechnol Biochem. 2009;73:245–59.
DMK is very thankful for the scholarship given by the Rotary Yoneyama Memorial Foundation.
This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology, Japan; in part by Special Coordination Funds for Promoting Science and Technology, Creation of Innovative Centers for Advanced Interdisciplinary Research Areas, by KAKENHI (26660067).
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Kang, DM., Michon, C., Morinaga, T. et al. Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenase. BMC Microbiol 17, 154 (2017). https://doi.org/10.1186/s12866-017-1065-8