Table 1 Bacterial strains and plasmids
Strain or plasmid | Description | Source or reference |
|---|---|---|
E. coli | ||
 DH5α | supE44 ΔlacU169 (Φ80 lacZΔM15) hsdR17 recA1 gyrA96 thi-1 relA | [24] |
 BL21 | F − ompT hsdS Β (rΒ −mΒ −) dcm gal (DE3) tonA | Merck Millipore |
B. subtilis | ||
 168 | trpC2 | Laboratory stock |
  BFS3018 | trpC2 iolX::pMUTIN4 | [12] |
  CM101 | trpC2 ΔyisR | This study |
  CM102 | trpC2 ΔiolQ | This study |
Plasmid | ||
  pMD20 | TA-cloning vector, amp | Takara Bio |
  pET-30a | pET system expression vector, kan | Merck Millipore |
  pET-iolQ | pET-30 derivative to express iolQ-His 6 | This study |
  pET-yisR | pET-30 derivative to express YisR-His 6 | This study |