Fig. 6

Primer extension analysis of the iolX transcript. Total RNA samples extracted from strain 168 grown in S6 media containing 0.5% casamino acid, 0.005% tryptophan, not supplemented (lane 1) or with 10 mM SI (lane 2) were reverse transcribed to generate cDNA. Lanes G, A, T, and C are dideoxy sequencing ladders that correspond to the reverse transcript (lower strand) generated from the same primer used for the reverse transcription. The partial nucleotide sequence of the upper strand of the promoter region is shown on the left where the identified two 5′-ends of the transcripts from the promoters P1 and P2 are indicated in bold face, whereas the reverse transcripts corresponding to the promoter P1 and P2 are indicated by arrowheads on the right side