Fig. 5From: Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenaseElectrophoretic gel mobility shift assay. a Purification of IolQ-His6 (IolQ) and YisR-His6 (YisR). The purified proteins migrated to form the respective bands in SDS-PAGE with the expected sizes (arrowheads on the right). M, size markers. b Results of electrophoretic gel mobility shift assay of the interaction of IolQ-His6 with the fragment of the iolX promoter. The DNA fragments corresponding to the 200Â bp iolX promoter region (200Â bp of iolX) and the negative control 100Â bp fragment derived from the iolW coding region (100Â bp of N. C.) were incubated with various concentrations of IolQ-His6 as indicated (nM of IolQ) and subjected to non-denaturing PAGE. The bands representing IolQ-His6-DNA complexes are indicated as the IolQ-iolX complexBack to article page