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Table 2 Oligonucleotide primers

From: Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenase

Primer

Sequence (5′ → 3′)*

[FAM]iolX(+50)-R†

TAACCGAGCCTTCCTAATCC

[FAM]iolX(−250)-F†

GAGCTTGTAGTCAGACATTCT

DiolQAF

TGTCAAACAGGGAACGTTAT

DiolQAR

CGCTCATTAGCGGGCCATCCCTCGTCTGGTTATTG

DiolQBF

GCCCGCTAATGAGCG

DiolQBR

CTGATTGGGTAGGATCCCCGCATGGATGGAACAGTCGATA

DiolQCF

GCTTGAGTCAATTCCGCTGTCGATGAGCTCGGTTTTCAAATG

DiolQCR

CCCATCTCTTTTATCGGCTG

iolQBamHI-R

CGCGGATCCCGTAATCGGTGCTGCAAATC

iolQEcoRI-F

GGAATTCTAACCAGACGAGGGATGAAC

iolQNdeI-F

GGGAATTCCATATGATGAAAACAACAATTTACGATGT

iolQXhoI-R

CCGCTCGAGTCATGTGTTGAGCGGTGATG

DyisRAF

TTGACAATCACAATCATCGC

DyisRAR

GTTATTGAACTTTCCGGCTGTTTTTAAGTCGGATTTTTACAAGAAG

DyisRBF

CAGCCGGAAAGTTCAATAAC

DyisRBR

CTGATTGGGTAGGATCCCCGGCATTTCTGTCGAGCAATTT

DyisRCF

GCTTGAGTCAATTCCGCTGTCGTGTCAAACAGGGAACGTTAT

DyisRCR

TCCGGTATTCAATTGGTGAA

GMSA-Nega-F

TTTTCACGGGCCGCTGCT

GMSA-Nega-R

CTCAGCATCTGGAAAATCCC

iolX (+50)-R

TAACCGAGCCTTCCTAATCC

iolX (−1)-R

GTCCCATCCTCTCCTTTATC

iolX (−200)-F

ATGAGCGGGTTTTTTCATTATG

iolX (−250)-F

GAGCTTGTAGTCAGACATTCT

MazFbw

GGGGATCCTACCCAATCAG

MazFfw

AGCGGAATTGACTCAAGC

NiolX

CGGATCGACGCTGGAGAAA

NiolXDIG

TAATACGACTCACTATAGGGAGCCGATAGGATGGTCACAT

PiolX400-F

TAGCCCAGCCGATAAAAGAG

PiolX400-R

TAACCGAGCCTTCCTAATCC

yisR (−1)-R

TTGAATCATCCTCCTTTTTAAGT

yisR (−200)-F

CAAGTAAGCGAAAATAATGAGAA

yisRBamHI-R

CGCGGATCCCGAGCGACAGATCCTTGATT

yisREcoRI-F

GGAATTCCTTTCTCCCGGTCTTGAACA

yisRNdeI-F

GGGAATTCCATATGATGCCTCGCATCCTGTTTAC

yisRXhoI-R

CCGCTCGAGTTATTGAACTTTCCGGCTGAC

  1. *Restriction enzyme recognition sites and T7 RNA polymerase promoter-tag sequence are underlined and italicized, respectively
  2. †These primers were 5′-6-[FAM]-labeled