Fig. 7

DNase I foot printing of IolQ-His₆ on the iolX promoter region. DNase I foot printing of the upper a and lower b strands. Sequence data are shown on the top and below are fragment analysis data acquired using various concentrations of IolQ-His₆ as indicated on the right. c Summary of DNase I foot printing data. The nucleotide sequences (upper and lower strands) of the DNA fragment that correspond to the 200 bp iolX promoter region used for the electrophoretic gel mobility shift assay are shown. Transcription initiation sites +1 (P1) and +1 (P2) and their corresponding −35 and −10 regions are indicated. The protected regions with higher and lower affinities are indicated by black and gray bars, respectively. The conserved sequences within the protected regions are boxed. The cre sites are indicated by the dashed bars between the upper and lower strand sequences within the two regions for IolQ binding