- Research article
- Open Access
Genome-wide identification of Bacillus subtilis Zur-binding sites associated with a Zur box expands its known regulatory network
© Prestel et al.; licensee BioMed Central. 2015
- Received: 23 September 2014
- Accepted: 13 January 2015
- Published: 4 February 2015
The Bacillus subtilis Zur transcription factor recognizes a specific DNA motif, the Zur box, to repress expression of genes in response to zinc availability. Although several Zur-regulated genes are well characterized, a genome-wide mapping of Zur-binding sites is needed to define further the set of genes directly regulated by this protein.
Using chromatin immunoprecipitation coupled with hybridization to DNA tiling arrays (ChIP-on-chip), we reported the identification of 80 inter- and intragenic chromosomal sites bound by Zur. Seven Zur-binding regions constitute the Zur primary regulon while 35 newly identified targets were associated with a predicted Zur box. Using transcriptional fusions an intragenic Zur box was showed to promote a full Zur-mediated repression when placed within a promoter region. In addition, intragenic Zur boxes appeared to mediate a transcriptional cis-repressive effect (4- to 9-fold) but the function of Zur at these sites remains unclear. Zur binding to intragenic Zur boxes could prime an intricate mechanisms of regulation of the transcription elongation, possibly with other transcriptional factors. However, the disruption of zinc homeostasis in Δzur cells likely affects many cellular processes masking direct Zur-dependent effects. Finally, most Zur-binding sites were located near or within genes responsive to disulfide stress. These findings expand the potential Zur regulon and reveal unknown interconnections between zinc and redox homeostasis regulatory networks.
Our findings considerably expand the potential Zur regulon, and reveal a new level of complexity in Zur binding to its targets via a Zur box motif and via a yet unknown mechanism that remains to be characterized.
- Zur regulator
- B. subtilis
- Zinc homeostasis
- Disulfide stress
Zinc is an essential trace element for all forms of life. It serves as structural scaffold for protein folding and as cofactor for many enzymes and DNA-binding proteins [1,2]. However, due to its toxicity, mechanisms for zinc acquisition and efflux are tightly regulated according to metal ion requirements [3-5].
In the Gram positive bacterium Bacillus subtilis, transcription of genes involved in zinc homeostasis is regulated by Zur, a metalloprotein that binds Zn(II) as corepressor [6-9]. In vivo, Zur forms a homodimer that binds to a conserved DNA motif, the Zur box, which overlaps the σA-type promoter elements in target genes . DNA-binding studies demonstrated that Zur requires a minimal 9-1-9 inverted repeat motif for high-affinity binding . A stepwise activation model predicts that Zur may respond to a wide range of intracellular Zn(II) concentrations to gradually repress the Zur regulon . Zur represses expression of genes encoding a high-affinity zinc ABC transporter ZnuACB , a putative low affinity zinc uptake system YciBC , a GTP cyclohydrolase IB involved in folate biosynthesis FolEB , and zinc-independent alternative ribosomal proteins (RpmEB, RpmGC, and RpsNB) [14-18]. In B. subtilis, zinc is also imported by the P-type ATPase ZosA, whose expression is controlled by the peroxide-sensing regulator, PerR . Induction of ZosA in response to hydrogen peroxide stress leads to Zn(II) uptake, which plays an important protective role against oxidative stress damage . Both ZosA and ZnuACB zinc transporters are involved in the competence developmental process . Zinc homeostasis is also maintained in B. subtilis thanks to a zinc-inducible efflux pump CzcD important for growth in the presence of high concentration of Zn(II) [5,21]. Expression of this system is regulated at the transcriptional level by the metalloregulator CzrA .
Despite knowledge of Zur-mediated regulation of zinc homeostasis, a global identification of the genes directly under Zur control is still missing. Here, we used chromatin immunoprecipitation of Zur-DNA complexes coupled with hybridization of DNA to tiled oligonucleotides arrays (ChIP-on-chip) to identify regions enriched for Zur DNA-binding sites in vivo, at the genomic scale. We provide evidence that Zur binds to 80 regions on the chromosome, including previously known promoter regions of the Zur primary regulon as well as a number of inter- and intragenic regions. Half of the newly identified binding sites is associated with a predicted Zur box. We showed that an intragenic Zur box was functional to mediate a Zur-dependent repression when inserted in a promoter region. In addition, several intragenic Zur boxes were able to act as transcriptional cis-repressive element but the direct role of Zur at intragenic sites remains unclear. Finally, our study suggests that Zur binding to newly identified targets could be primed to fine-tune gene expression in interplay with other transcription factors in response to specific conditions such as the disulfide stress.
C-terminally SPA-tagged Zur is a functional regulator
B. subtilis strains used in this study
(Nicolas et al., )
amyE::pyciC’-lacZ cat zur-spa erm
amyE::pyciC’-lacZ cat Δzur::aphA3
amyE::pAyrpE’-lacZ cat Δzur::aphA3
amyE::pByrpE’-lacZ cat Δzur::aphA3
amyE::pAymaD'-luc cat Δspx::spc
amyE::pAymaD'-luc cat Δzur::aphA3
amyE::pBymaD'-luc cat Δzur::aphA3
amyE::pBymaD’-lacZ cat Δspx::spc
We further tested the effect of ion starvation on yciC'-lacZ expression in the same genetic backgrounds. The strains were cultivated in MS medium and samples were spread onto solid medium containing X-gal. A drop of 100 μM EDTA, an ion chelating agent, was deposited at the center of the plates. After incubation, a characteristic blue ring was observed around the EDTA drop (Additional file 1: Figure S1) indicating that ion deficiency induced yciC expression in cells synthesizing Zur as well as ZurSPA proteins. The binding of ZurSPA to the yciC promoter region was reversible upon metal ion starvation. We concluded from this data that the ZurSPA fusion protein was functional for transcriptional regulation.
Genome-wide mapping of Zur binding sites
We further compared our data with previous in silico studies . The promoter-proximal Zur-binding sites upstream of yrpE and the pseudogene S903 (also named rpmGC) exhibited Zur box motifs. We then tested expression of the yrpE gene in wild-type and Δzur cells by using a transcriptional fusion between the yrpE promoter region and the lacZ reporter gene. Expression of the PyrpE promoter was 30-fold increased in a zur mutant compared to the wild-type during the exponential phase of growth (Additional file 1: Figure S1). These results demonstrated the Zur-dependent regulation of yrpE. We also detected Zur binding upstream of the short yczL gene, encoding a protein of unknown function. Expression of yczL is co-regulated with yciC and is driven from a single promoter leading to the yczLyciC transcript . Altogether, these findings delineated the Zur primary regulon, which is now composed of 11 genes expressed from 7 distinct promoters (znuABC, folEByciB, rpsNB, rpmEB, rpmGC, yczLyciC and yrpE) fulfilling three criteria: (i) in vivo Zur binding in ChIP-on-chip experiments; (ii) presence of a Zur box; (iii) Zur-dependent regulation of expression.
In addition, 37 additional promoter-proximal Zur binding sites were detected less than 200 base-pairs upstream of a translational start site (Additional file 2: Table S1) suggesting a Zur-dependent expression and existence of new candidates to be part of the Zur regulon. The expression of all the genes belonging to the Zur primary regulon displayed a similar expression profile whereas the expression of the genes closed to the 37 newly identified promoter-proximal sites did not appear correlated with the Zur primary regulon . These genes might be controlled by Zur under specific unknown conditions. The presence of predicted Zur boxes in these regions is discussed below.
Finally, 35 peaks were located within intragenic regions more than 200 bp downstream a start codon (Figure 1B). The location of these sites was intriguing since none Zur intragenic binding site has so far been described.
Prediction of Zur boxes within Zur-binding sites
Next, the Zur box consensus was submitted to FIMO  to identify putative Zur box in all the B. subtilis genome. Hits with a p value of ≤ 10−6 were regarded as significant, resulting in 167 candidates including the 40 Zur boxes associated with in vivo Zur binding (see above) (Additional file 4: Table S3). These results showed that only a set of potential Zur boxes were bound by Zur, at least in the conditions used.
Half of the Zur-binding sites detected by ChIP-on-chip did not display a significant match to the Zur box consensus. As shown in Figure 4, these sites were located in inter- as well as in intragenic with some high ChIPScores (>15). Using MEME, we were unable to identify a common DNA sequence motif among Zur targets that lack a canonical Zur box motif.
The intragenic Zur box from ymaD promotes a Zur-mediated repression when placed within a promoter region
We further investigated the functionality of an intragenic Zur box to be recognized by the wild-type Zur protein to promote a repressive effect on transcription. We chose the intragenic Zur box located in the ymaD gene encoding a putative peroxiredoxin. Peroxiredoxins are important for antioxidant defense by reducing hydrogen peroxide (H2O2), which induces the zinc uptake system ZosA in B. subtilis . A ΔymaD deleted strain (Table 1) was constructed and the sensitivity of this ymaD mutant to H2O2 was tested in liquid medium. After 30 min of growth in the presence of 400 μM H2O2, 0.005% and 1% survival was observed for the mutant and the wild-type cells, respectively (Additional file 5: Figure S2). Thus, deletion of the ymaD gene increased the sensitivity of the cells to H2O2, indicating that YmaD plays a role in protecting cells against oxidative stress.
Intragenic Zur boxes: a role in transcription elongation?
Zur-binding sites overlap genes responding to disulfide stress
Our analyses revealed that a large overlap existed between the location of Zur-binding sites and genes whose expression responds to disulfide stress . Zur-binding sites were located less than 400 bp upstream of the translational start site or in the coding sequences of 31-activated and 19-repressed genes in response to diamide stress (Additional file 6: Table S4). Furthermore, Zur-binding sites are associated to 10 genes (citR, cysK, ilvA, katA, S1408, pps, ybxG, ymaD, yusD, yvcI) reported to be directly regulated by Spx , suggesting that Zur- and Spx-mediated regulations partially overlap.
No expression of pAymaD'-luc and pBymaD'-luc was detected in a Δspx mutant (Figure 7A), in keeping with the Spx-dependent activation of ymaD . In the wild-type cells, 0.1 or 0.5 mM diamide treatment reproducibly increased pAymaD'-luc expression (Figure 7B). This indicated that Zur binding to the Zur box of pAymaD did not interfere woth Spx activity. The same pattern of induction was observed with pBymaD (Figure 7B). Thus, the intragenic Zur box did not appear involved in the Spx-dependent upregulation.
Using the ChIP-on-chip methodology, we identified 80 enriched DNA-regions in the B. subtilis chromosome that are reproducibly bound by the Zur repressor under abundant zinc growth conditions. We recovered the known Zur regulon and confirmed that the predicted Zur boxes present upstream of yrpE and rpmGC [17,25] are functional binding sites in vivo. These data allow to define the Zur primary regulon, which is now composed of 7 transcription units. Consequently, the whole Zur binding sites identified by ChIP-on-chip appear relevant. Remarkably, a second set of 33 newly identified sites bound by Zur contained a Zur box motif, which differs from the Fur and PerR boxes at positions 4, 5, 15, and 16 and displays conservation of bases at flanking positions that are not strongly conserved in Fur and PerR boxes (positions 1,2,3 and 18, 19, 20) (Figure 2) [11,29]. The last set of Zur binding DNA sites did not contain any direct-repeat sequence or any common motif by the standard bioinformatic methods that we used, suggesting that Zur recognizes degenerated Zur box sequences or that other factors are required for Zur binding at these sites. Interestingly, the Zur binding sites with and without predicted Zur box exhibit a similar distribution relative to coding sequences, with an enrichment around the translation start site (Figure 4), suggesting that both types of sites are functionally similar.
A surprising result from this study was the finding that 35 Zur binding sites are located in intragenic regions. Sixteen of those contain a predicted Zur box. This contrasts sharply with in silico studies, which tend to consider transcription factor-targets within coding sequences as artefacts. Analysis with transcriptional fusions using the lacZ reporter gene allowed us to show that the intragenic Zur box sequence from the ymaD gene was fully functional for Zur-mediated repression when placed in a promoter region. Thus, the experimental strategies used in this study revealed unexpected and functional Zur binding sites. In addition, we showed that the intragenic Zur box within ymaD, ydeO, ywhC and ktrD genes had a cis-repressive effect (3- to 10-fold) on transcription. Expression of these genes was also tested in a Δzur mutant but similar levels of expression was observed in Δzur and wild-type cells (data not shown). As disruption of zinc homeostasis in Δzur cells likely affects many cellular processes, the direct role of Zur binding to the intragenic Zur boxes could be masked by other regulatory effects.
Our study highlights the presence of Zur boxes within a subset of genes encoding functions related to metal ion homeostasis or oxidative stress. We showed that the ymaD gene codes for putative peroxiredoxin-related protein, which plays a role in protecting cells against oxidative stress. Interestingly, ymaD is also under direct regulation by Spx  a global transcription key regulator for maintaining redox homeostasis of B. subtilis cells exposed to disulfide stress . The intragenic Zur box of ymaD did not appear to play a direct role on the Spx-dependent regulation. Remarkably, we observed that the ymaD transient induction upon exposure to diamide was abolished in Δzur cells (data not shown) pointing to an interconnection of the Zur- and Spx-mediated responses. As the degradation of Spx is ensured by the ClpXP protease , disruption of zinc homeostasis in Δzur cells could impact on the activity of the Zn-dependent protein ClpX and, as a consequence, on the turnover of Spx. Overall, it was not possible to conclude about the direct role of Zur to mediate a regulation via the intragenic Zur boxes because of Δzur deletion may entail significantly changes in other transcription factors activity. In this intricate regulatory network, the binding of Zur to intragenic Zur boxes may contribute to fine-tune gene expression in response to zinc availability.
The interconnection between Zur and Spx may involve more than one gene as Zur binding sites were detected within or near 10 Spx-regulated genes and overall near 50 genes responsive to diamide stress (Additional file 6: Table S4). Overlap between disulfide and oxidative stress responses was previously identified in B. subtilis for the katA gene encoding a catalase. Expression of katA is under the dual control of PerR  and Spx  regulators. In addition, a regulatory interplay between the responses to zinc deprivation and disulfide stress has been described in Streptomyces coelicolor, where the activity of the thiol-disulfide metabolism regulator σR is induced upon zinc limitation . Our results emphasize the complex interplay between the regulatory networks controlling zinc homeostasis and redox homeostasis, especially the oxidative and disulfide stress responses.
The Chip-on-chip approach used in this study allowed to considerably expand the catalogue of in vivo Zur-binding sites to 80 inter- as well as intragenic regions. Half of those is associated with an in silico predicted Zur box. The binding of Zur to the newly identified targets may contribute to fine-tune gene expression under specific conditions, our results highlighting a complex link between Zur and the disulfide stress response. Intragenic Zur boxes could be involved in an intricate mechanisms of regulation of the transcription elongation, possibly with other transcriptional factors. Future investigations will be required to investigate the role of Zur binding sites in transcriptional regulation.
Bacterial strains and growth conditions
The B. subtilis strains used in this work are listed in Table 1. E. coli and B. subtilis cells were grown in Luria-Bertani (LB) medium or in MS medium containing 62 mM K2HPO4, 44 mM KH2PO4, 17 mM trisodium citrate, 11 mM K2SO4, 0.4% glucose, 0.06% L-glutamine, 0.01% L-tryptophan, 0.1% casamino acids, 1 mM MgSO4, 1 mM CaCl2, 100 μM FeCl3 citrate, 112 μM ZnCl2; 5 μM MnCl2; 2.5 μM CuCl2. Antibiotics were added at the following concentrations when required: 100 μg ampicillin ml−1; 5 μg kanamycin ml−1; 10 μg erythromycin ml−1; 5 μg chloramphenicol ml−1; 60 μg spectinomycin ml−1. Solid media were prepared by addition of 20 g Agar noble l−1 (Difco). Standard procedures were used to transform E. coli  and B. subtilis .
DNA manipulations and cloning procedures were performed as described elsewhere  according to standard procedures. Restriction enzymes, Pfu DNA polymerase and phage T4 DNA ligase were used as recommended by the manufacturer (Biolabs). DNA fragments were purified from agarose gels using the QIAquick kit (Qiagen).
Construction of plasmids and strains
A B. subtilis strain expressing a C-terminal SPA-tagged Zur protein (hereafter ZurSPA) was constructed by chromosomal integration of a translational fusion between the zur coding sequence and the sequential peptide affinity (SPA) tag sequence [39,40], resulting in the BSAS36 strain expressing ZurSPA under the control of its native promoter as unique source of Zur. In this purpose, the zur coding sequence (from nucleotide +13 to + 435 relative to the translational start site) was amplified by PCR with oligonucleotides creating an Acc651 restriction site at the 5′ end (5′-GGAATTGGTACCgaagcgctgaacctattaaaa-3′) and a NcoI restriction site at the 3′ end of the fragment (5′-GGAATTCCATGGcgcagtagtgttttcttggtt-3′). The PCR product was cloned into plasmid pMUTIN-SPA subsequent to digestion with Acc651 and NcoI . The resulting plasmid was used to transform B. subtilis and to select for erythromycin-resistance. Integration was confirmed by PCR and verified by DNA sequencing.
The zur mutant BSAS45 was constructed by homologous replacement of the Zur coding sequence with the kanamycin-resistance gene aphA3 using a joining PCR technique . The aphA3 gene was first amplified. The region upstream of the zur gene (nucleotides −887 to +65 relative to the translational start site) was amplified by PCR with a 21 bp aphA3 fragment at its 3′ end. The region downstream of zur (nucleotides +366 to +1321) was amplified with a 21 bp aphA3 fragment at its 5′ end. The three DNA fragments were combined and then a PCR reaction was performed with the two external oligonucleotides. The final product, corresponding to the two regions flanking zur with the inserted aphA3 cassette in between, was purified from a gel and used to transform B. subtilis. Integration and deletion were confirmed by PCR and verified by DNA sequencing. The ymaD mutant BSAS239 was constructed by the same strategy. The region upstream of the ymaD gene (nucleotides −921 to +90 relative to the translational start site) was amplified by PCR with a 21 bp aphA3 fragment at its 3′ end. The region downstream of ymaD (nucleotides +400 to +1431) was amplified with a 21 bp aphA3 fragment at its 5′ end. The joining PCR, corresponding to the two regions flanking ymaD with the inserted aphA3 cassette in between was used to transform B. subtilis.
The spx mutant BSAS296 was constructed by homologous replacement of the Spx coding sequence with a spectinomycin-resistance gene spc. The spc gene was first amplified. The region upstream of the spx gene (nucleotides −861 to +57 relative to the translational start site) was amplified by PCR with a 21 bp spc fragment at its 3′ end. The region downstream of spx (nucleotides +348 to +1270) was amplified with a 21 bp spc fragment at its 5′ end. The three DNA fragments were combined and then a PCR reaction was performed with the two external oligonucleotides. The final product, corresponding to the two regions flanking spx with the inserted spc cassette in between, was purified from a gel and used to transform B. subtilis. Integration and deletion were confirmed by PCR and verified by DNA sequencing.
To construct transcriptional fusions with the lacZ reporter gene, DNA fragments corresponding to the various promoter regions under investigation were amplified by PCR. Oligonucleotides were used to create an EcoRI restriction site at the 5′ end and a BamHI restriction site at the 3′ end of the fragments. PCR products were cloned into plasmid pAC6 subsequent to digestion with EcoRI and BamHI . In this way, the promoter region of yciC (from nucleotide −312 to −1 relative to the translational start site) was fused with the lacZ reporter gene. The transcriptional fusions with the lacZ gene were subsequently integrated at the amyE locus of B. subtilis (Table 1). To generate pBymaD, pBydeO, pBywhC and pBktrD fusions, we used large primers introducing point-mutations in the Zur box motifs (Figure 6A). The resulting constructs were verified by DNA sequencing. β-galactosidase specific activities were measured during exponential phase growth in LB medium, as described by Miller with cell extracts obtained by lysozyme treatment . One unit of β-galactosidase activity was defined as the amount of enzyme that produces 1 nmol o-nitrophenol min-1 at 28°C. The mean values and standard deviations of at least three independent experiments are shown.
To construct transcriptional fusions with the luc reporter gene, we used the assembly Gibson's procedure  to obtain transcriptional fusions with luc instead of the lacZ gene. The PUC18cm-luc plasmid  was used as template to amplify the luc reporter gene. The sequence of the resulting constructs were verified by DNA sequencing. The mean values and standard deviations of at least three independent experiments are shown.
For the detection of luciferase activity, strains were first grown in LB medium to an optical density at 600 nm (OD600) of 2. Cells were then centrifuged and resuspended in fresh LB medium, adjusting all the cultures to an OD600 of 1. These pre-cultures were then diluted 20 fold in fresh LB medium and 200 μl was distributed in each of two wells in a 96-well black plate (Corning). 10 μl of luciferin were added to each well to reach a final concentration of 1.5 mg/ml (4.7 mM). The cultures were incubated at 37°C with agitation in a PerkinElmer Envision 2104 Multilabel Reader equipped with an enhanced sensitivity photomultiplier for luminometry. The temperature of the clear plastic lid was maintained at 38°C to avoid condensation. Relative Luminescence Unit (RLU) and OD600 were measured at 5 min intervals.
Genome-wide determination of the Zur-binding sites by ChIP-on-chip
Chromatin Immnunoprecipitation assays were performed to measure the chromosome-wide DNA-binding profiles of Zur, as described previously . Briefly, strain BSAS36 was cultivated at 37°C until an OD600 of 0.6 in LB medium with 1 μg erythromycin ml−1. Cells were treated with formaldehyde, cellular DNA was extracted and sonicated, and an antibody against the SPA-tag was used to preferentially purify the DNA regions specifically cross-linked to ZurSPA. The immuno-precipitated DNA (IP) and the control whole cell DNA extract (WCE) were labeled with Cy3 and Cy5, respectively, and co-hybridized to the B. subtilis Roche-NimbleGen tiled microarrays .
Peak sequence extraction and analysis
Identification of peaks corresponding to chromosomal Zur binding sites was performed as described in . IP/WCE ratios (log2) were corrected for dye bias using Loess regression on the MA plot. The signal was smoothed by two rounds of sliding window averaging (29 probes, around 320 bp). Maxima (or minima) were defined as probes for which the smoothed signal is the highest (or lowest respectively) into the window used for smoothing. Peaks within the same 300 bp window were merged. The peak height was calculated as the log2 ratio difference between the smoothed signal values of the maxima and the adjacent minima. In order to quantify enrichment of Zur-bound DNA regions, the signal was smoothed and a ChipScore was calculated as described by Buescher et al. . Briefly, this score is based on the distribution of the peak height values and estimates for each peak its relative distance from the median (ChipScore = [height-median]/[upperquartile-median]). Only the regions associated with a peak scoring ≥ 4 [a threshold determined empirically from ChIP–on-chip experiments with the transcription factor CcpA ] in both replicates were considered as putative Zur-binding sites in the subsequent analyses.
The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE64671 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64671).
We would like to thank Dr Etienne Dervyn for the help with transcriptomic analyses and Dr Nathalie Pigeonneau for the help with ChIP-on-chip data analyses. We are grateful to Dr Elena Bidnenko and Dr Olivier Delumeau for careful reading of the manuscript.
- McCall KA, Huang CC, Fierke CA. Function and Mechanism of Zinc Metalloenzymes. J Nutr. 2000;130(5):1437S–46.PubMedGoogle Scholar
- Krishna SS, Majumdar I, Grishin NV. Structural classification of zinc fingers. Nucleic Acids Res. 2003;31(2):532–50.View ArticlePubMed CentralPubMedGoogle Scholar
- McDevitt CA, Ogunniyi AD, Valkov E, Lawrence MC, Kobe B, McEwan AG, et al. A molecular mechanism for bacterial susceptibility to zinc. PLoS Pathog. 2011;7(11):e1002357.View ArticlePubMed CentralPubMedGoogle Scholar
- Blencowe DK, Morby AP. Zn(II) metabolism in prokaryotes. FEMS Microbiol Rev. 2003;27(2–3):291–311.View ArticlePubMedGoogle Scholar
- Moore CM, Gaballa A, Hui M, Ye RW, Helmann JD. Genetic and physiological responses of Bacillus subtilis to metal ion stress. Mol Microbiol. 2005;57(1):27–40.View ArticlePubMedGoogle Scholar
- Patzer SI, Hantke K. The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli. Mol Microbiol. 1998;28(6):1199–210.View ArticlePubMedGoogle Scholar
- Gaballa A, Helmann JD. Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis. J Bacteriol. 1998;180(22):5815–21.PubMed CentralPubMedGoogle Scholar
- Outten CE, Tobin DA, Penner-Hahn JE, O'Halloran TV. Characterization of the metal receptor sites in Escherichia coli Zur, an ultrasensitive zinc(II) metalloregulatory protein. Biochemistry. 2001;40(35):10417–23.View ArticlePubMedGoogle Scholar
- Lee JW, Helmann JD. Functional specialization within the Fur family of metalloregulators. Biometals. 2007;20(3–4):485–99. Epub 2007 Jan 2010.View ArticlePubMedGoogle Scholar
- Gaballa A, Wang T, Ye RW, Helmann JD. Functional analysis of the Bacillus subtilis Zur regulon. J Bacteriol. 2002;184(23):6508–14.View ArticlePubMed CentralPubMedGoogle Scholar
- Gabriel SE, Miyagi F, Gaballa A, Helmann JD. Regulation of the Bacillus subtilis yciC gene and insights into the DNA-binding specificity of the zinc-sensing metalloregulator Zur. J Bacteriol. 2008;190(10):3482–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Ma Z, Gabriel SE, Helmann JD. Sequential binding and sensing of Zn(II) by Bacillus subtilis Zur. Nucleic Acids Res. 2011;39(21):9130–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Sankaran B, Bonnett SA, Shah K, Gabriel S, Reddy R, Schimmel P, et al. Zinc-independent folate biosynthesis: genetic, biochemical, and structural investigations reveal new metal dependence for GTP cyclohydrolase IB. J Bacteriol. 2009;191(22):6936–49.View ArticlePubMed CentralPubMedGoogle Scholar
- Nanamiya H, Akanuma G, Natori Y, Murayama R, Kosono S, Kudo T, et al. Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome. Mol Microbiol. 2004;52(1):273–83.View ArticlePubMedGoogle Scholar
- Akanuma G, Nanamiya H, Natori Y, Nomura N, Kawamura F. Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis. J Bacteriol. 2006;188(7):2715–20.View ArticlePubMed CentralPubMedGoogle Scholar
- Nanamiya H, Kawamura F. Towards an elucidation of the roles of the ribosome during different growth phases in Bacillus subtilis. Biosci Biotechnol Biochem. 2010;74(3):451–61.View ArticlePubMedGoogle Scholar
- Gabriel SE, Helmann JD. Contributions of Zur-controlled ribosomal proteins to growth under zinc starvation conditions. J Bacteriol. 2009;191(19):6116–22.View ArticlePubMed CentralPubMedGoogle Scholar
- Natori Y, Nanamiya H, Akanuma G, Kosono S, Kudo T, Ochi K, et al. A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis. Mol Microbiol. 2007;63(1):294–307.View ArticlePubMedGoogle Scholar
- Gaballa A, Helmann JD. A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis. Mol Microbiol. 2002;45(4):997–1005.View ArticlePubMedGoogle Scholar
- Ogura M. ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis. J Biochem. 2011;150(6):615–25.View ArticlePubMedGoogle Scholar
- Moore CM, Helmann JD. Metal ion homeostasis in Bacillus subtilis. Curr Opin Microbiol. 2005;8(2):188–95.View ArticlePubMedGoogle Scholar
- Michna RH, Commichau FM, Todter D, Zschiedrich CP, Stulke J. SubtiWiki-a database for the model organism Bacillus subtilis that links pathway, interaction and expression information. Nucleic Acids Res. 2013;42:D692–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, et al. Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism. Science. 2012;335:1099–103.View ArticlePubMedGoogle Scholar
- Rochat T, Nicolas P, Delumeau O, Rabatinova A, Korelusova J, Leduc A, et al. Genome-wide identification of genes directly regulated by the pleiotropic transcription factor Spx in Bacillus subtilis. Nucleic Acids Res. 2012;40(19):9571–83.View ArticlePubMed CentralPubMedGoogle Scholar
- Panina EM, Mironov AA, Gelfand MS. Comparative genomics of bacterial zinc regulons: enhanced ion transport, pathogenesis, and rearrangement of ribosomal proteins. Proc Natl Acad Sci. 2003;100(17):9912–7.View ArticlePubMed CentralPubMedGoogle Scholar
- Nicolas P, Mader U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, et al. Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science. 2012;335:1103–6.View ArticlePubMedGoogle Scholar
- Baichoo N, Wang T, Ye R, Helmann JD. Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon. Mol Microbiol. 2002;45(6):1613–29.View ArticlePubMedGoogle Scholar
- Fillat MF. The FUR (ferric uptake regulator) superfamily: diversity and versatility of key transcriptional regulators. Arch Biochem Biophys. 2014;546:41–52.View ArticlePubMedGoogle Scholar
- Fuangthong M, Helmann JD. Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis. J Bacteriol. 2003;185(21):6348–57.View ArticlePubMed CentralPubMedGoogle Scholar
- Bailey TL, Williams N, Misleh C, Li WW. MEME: discovering and analyzing DNA and protein sequence motifs. Nucleic Acids Res. 2006;34:W369–73.View ArticlePubMed CentralPubMedGoogle Scholar
- Grant CE, Bailey TL, Noble WS. FIMO: scanning for occurrences of a given motif. Bioinformatics. 2011;27(7):1017–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Choi SK, Saier Jr MH. Regulation of sigL expression by the catabolite control protein CcpA involves a roadblock mechanism in Bacillus subtilis: potential connection between carbon and nitrogen metabolism. J Bacteriol. 2005;187(19):6856–61.View ArticlePubMed CentralPubMedGoogle Scholar
- Belitsky BR, Sonenshein AL. Roadblock repression of transcription by Bacillus subtilis CodY. J Mol Biol. 2011;411:729–43.View ArticlePubMed CentralPubMedGoogle Scholar
- Nakano S, Erwin KN, Ralle M, Zuber P. Redox-sensitive transcriptional control by a thiol/disulphide switch in the global regulator, Spx. Mol Microbiol. 2005;55(2):498–510.View ArticlePubMedGoogle Scholar
- Zhang Y, Zuber P. Requirement of the zinc-binding domain of ClpX for Spx proteolysis in Bacillus subtilis and effects of disulfide stress on ClpXP activity. J Bacteriol. 2007;189(21):7669–80.View ArticlePubMed CentralPubMedGoogle Scholar
- Owen GA, Pascoe B, Kallifidas D, Paget MS. Zinc-responsive regulation of alternative ribosomal protein genes in Streptomyces coelicolor involves zur and sigmaR. J Bacteriol. 2007;189(11):4078–86.View ArticlePubMed CentralPubMedGoogle Scholar
- Sambrook J, Fristch EF, Maniatis T, editors. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Press; 1989.Google Scholar
- Kunst F, Rapoport G. Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis. J Bacteriol. 1995;177(9):2403–7.PubMed CentralPubMedGoogle Scholar
- Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, et al. Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature. 2005;433(7025):531–7.View ArticlePubMedGoogle Scholar
- Zeghouf M, Li J, Butland G, Borkowska A, Canadien V, Richards D, et al. Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes. J Proteome Res. 2004;3(3):463–8.View ArticlePubMedGoogle Scholar
- Lecointe F, Serena C, Velten M, Costes A, McGovern S, Meile JC, et al. Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks. Embo J. 2007;26(19):4239–51.View ArticlePubMed CentralPubMedGoogle Scholar
- Wach A. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast. 1996;12(3):259–65.View ArticlePubMedGoogle Scholar
- Stülke J, Martin-Verstraete I, Zagorec M, Rose M, Klier A, Rapoport G. Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT. Mol Microbiol. 1997;25(1):65–78.View ArticlePubMedGoogle Scholar
- Miller JH. Assay of B-galactosidase. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1972.Google Scholar
- Gibson DG, Young L, Chuang RY, Venter JC, Hutchison 3rd CA, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6(5):343–5.View ArticlePubMedGoogle Scholar
- Mirouze N, Prepiak P, Dubnau D. Fluctuations in spo0A transcription control rare developmental transitions in Bacillus subtilis. PLoS Genet. 2011;7(4):e1002048.View ArticlePubMed CentralPubMedGoogle Scholar
- Rasmussen S, Nielsen HB, Jarmer H. The transcriptionally active regions in the genome of Bacillus subtilis. Mol Microbiol. 2009;73:1043–57.View ArticlePubMed CentralPubMedGoogle Scholar
- Reppas NB, Wade JT, Church GM, Struhl K. The transition between transcriptional initiation and elongation in E. coli is highly variable and often rate limiting. Mol Cell. 2006;24:747–57.View ArticlePubMedGoogle Scholar
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