Figure 6
Expression of various transcriptional fusions in the wild-type and the Δ zur strains. (A) Scheme of the DNA regions inserted upstream from the lacZ reporter gene. Nucleotides are numbered relative to the translational start site of the ymaD, ywhC, ydeO and ktrD genes. The native intragenic Zur boxes are indicated by black boxes. The mutated intragenic Zur boxes are indicated by white boxes. The generated point-mutations in the Zur box sequences are indicated in bold capital letters. (B) Cells were grown in LB medium. β-galactosidase activities were determined in extracts prepared from exponentially growing cells at OD600 of 0.6.