Genome-wide identification of Bacillus subtilis Zur-binding sites associated with a Zur box expands its known regulatory network

Table 1 B. subtilis strains used in this study

Strain	Genotype ^a	Source
BSB1	trp ⁺	(Nicolas et al., [26])
BSAS36	zur::zur-spa erm	This work
BSAS39	amyE::pyciC’-lacZ cat	This work
BSAS44	amyE::pyciC’-lacZ cat zur-spa erm	This work
BSAS45	Δzur::aphA3	This work
BSAS49	amyE::pyciC’-lacZ cat Δzur::aphA3	This work
BSAS225	amyE::pAymaD’-lacZ cat	This work
BSAS331	amyE::pBymaD’-lacZ cat	This work
BSAS227	amyE::pAydeO’-lacZ cat	This work
BSAS332	amyE::pBydeO’-lacZ cat	This work
BSAS229	amyE::pAywhC’-lacZ cat	This work
BSAS333	amyE::pBywhC’-lacZ cat	This work
BSAS239	ΔymaD::aphA3	This work
BSAS240	amyE::pAktrD’-lacZ cat	This work
BSAS329	amyE::pBktrD’-lacZ cat	This work
BSAS249	amyE::pAyrpE’-lacZ cat	This work
BSAS250	amyE::pAyrpE’-lacZ cat Δzur::aphA3	This work
BSAS251	amyE::pByrpE’-lacZ cat	This work
BSAS252	amyE::pByrpE’-lacZ cat Δzur::aphA3	This work
BSAS296	Δspx::spc	This work
BLUC201	amyE::pAymaD'-luc cat	This work
BLUC204	amyE::pAymaD'-luc cat Δspx::spc	This work
BLUC205	amyE::pAymaD'-luc cat Δzur::aphA3	This work
BLUC202	amyE::pBymaD'-luc cat	This work
BLUC205	amyE::pBymaD'-luc cat Δzur::aphA3	This work
BLUC227	amyE::pBymaD’-lacZ cat Δspx::spc	This work

^a cat, pC194 chloramphenicol acetyl-transferase gene; aphA3, Enterococcus faecalis kanamycin-resistance gene; erm, erythromycin-resistance gene; spc, Staphylococcus aureus spectinomycin-resistance gene.

ISSN: 1471-2180