Figure 7
Expression of ymaD in various genetic backgrounds and after diamide treatment. (A) Expression of pAymaD and pBymaD fusions in the wild-type (WT) strain (blue lines), in Δzur (red lines) or Δspx cells (black lines). Growth was monitored by measuring the optical density at 600 nm: grey line, wild-type. The Δzur and Δspx stains grew with the same growth profile than the wild-type. (B) Promoter activity of pAymaD and pBymaD without diamide treatment (blue lines), with 0.1 mM diamide (black lines) or with 0.5 mM diamide (green lines). Final concentration of 0.1 or 0.5 mM diamide was added at t = 1 hour when cells reached an OD600 of 0.2 in LB medium. For each strain, one representative curve, out of three independent replicates realized, is shown.