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Figure 5 | BMC Microbiology

Figure 5

From: Genome-wide identification of Bacillus subtilis Zur-binding sites associated with a Zur box expands its known regulatory network

Figure 5

Structure of the yrpE promoter region and expression of yrpE ’- lacZ transcriptional fusions. (A) Detailed view of the yrpE promoter region in the two transcriptional fusions. The σA-dependent recognition site is underlined, the ribosome-binding site is capitalized. The Zur box motif is bold. In pAyrpE, the Zur box motif is the native sequence. In pByrpE, the Zur box motif is the intragenic Zur box sequence from ymaD. The six nucleotides differences are indicated in bold capital letters. (B) Expression of the transcriptional pAyrpE’-lacZ and pByrpE’-lacZ fusions in the wild-type (WT) strain and the Δzur deleted mutant. Nucleotides are numbered relative to the translational start site of the yrpE gene. Location of the Zur motif is indicated by boxes. White box, native Zur box sequence from the yrpE promoter region; black box, Zur box sequence identified within the ymaD gene. β-galactosidase activities were measured at least 3 times independently.

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