- Research article
- Open Access
Corynebacterium glutamicum possesses β-N-acetylglucosaminidase
© The Author(s). 2016
Received: 16 January 2016
Accepted: 30 July 2016
Published: 5 August 2016
In Gram-positive Corynebacterium glutamicum and other members of the suborder Corynebacterianeae, which includes mycobacteria, cell elongation and peptidoglycan biosynthesis is mainly due to polar growth. C. glutamicum lacks an uptake system for the peptidoglycan constituent N-acetylglucosamine (GlcNAc), but is able to catabolize GlcNAc-6-phosphate. Due to its importance in white biotechnology and in order to ensure more sustainable processes based on non-food renewables and to reduce feedstock costs, C. glutamicum strains have previously been engineered to produce amino acids from GlcNAc. GlcNAc also is a constituent of chitin, but it is unknown if C. glutamicum possesses chitinolytic enzymes.
Chitin was shown here not to be growth substrate for C. glutamicum. However, its genome encodes a putative N-acetylglucosaminidase. The nagA 2 gene product was active as β-N-acetylglucosaminidase with 0.27 mM 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside as substrate supporting half-maximal activity. NagA2 was secreted into the culture medium when overproduced with TAT and Sec dependent signal peptides, while it remained cytoplasmic when overproduced without signal peptide. Heterologous expression of exochitinase gene chiB from Serratia marcescens resulted in chitinolytic activity and ChiB secretion was enhanced when a signal peptide from C. glutamicum was used. Colloidal chitin did not support growth of a strain secreting exochitinase ChiB and β-N-acetylglucosaminidase NagA2.
C. glutamicum possesses β-N-acetylglucosaminidase. In the wild type, β-N-acetylglucosaminidase activity was too low to be detected. However, overproduction of the enzyme fused to TAT or Sec signal peptides led to secretion of active β-N-acetylglucosaminidase. The finding that concomitant secretion of endogenous NagA2 and exochitinase ChiB from S. marcescens did not entail growth with colloidal chitin as sole or combined carbon source, may indicate the requirement for higher or additional enzyme activities such as processive chitinase or endochitinase activities.
Corynebacteria are Gram-positive microorganisms closely related to mycobacteria. C. glutamicum is considered a model organism for other members of the suborder Corynebacterianeae. Likewise, C. glutamicum is a model bacterium for white biotechnology since it is used for the production of amino acids and derived products . This bacterium can use a variety of carbon sources for growth and production, e.g. sugars (glucose, fructose, sucrose, maltose, ribose), alcohols (ethanol, myo-inositol) and organic acids (acetate, propionate, D- or L-lactate, gluconate, pyruvate) [2–6]. Metabolic engineering has been applied to C. glutamicum aimed at improving amino acid production, to enable production of novel compounds such as diamines [7, 8] or terpenoids [9–11], and to enable a flexible feedstock concept . Feedstock costs are an important part of the overall fermentation costs and access to a wide range of carbon substrates, in particular non-food renewables, is sought . Recombinant strains of C. glutamicum which can utilize glycerol [13, 14], starch [15, 16], arabinose and xylose derived from lignocellulosic biomass [17–21] or the amino sugars glucosamine (GlcN) and N-acetyl-glucosamine (GlcNAc) have been developed [22, 23]. GlcN and GlcNAc represent interesting feedstocks for biotechnological applications since they can be produced by acid hydrolysis of chitin, one of the major waste components generated from shellfish industry [24, 25]. Shrimp farming is a continuously growing sector concentrated in Southeast Asia and China, where every year around 4 million MT of shrimps are harvested, with a chitin-containing waste generation of around 40 % of shrimp dry weight [26, 27]. Thus, the amino sugar fraction of shellfish waste could represent an example of a sustainable, renewable feedstock for industrial fermentations, in particular for nitrogenous compounds such as amino acids and diamines.
C. glutamicum lacks the transporter for GlcNAc so that the heterologous overexpression of a specific transporter is necessary in order to achieve growth on this carbon source . GlcNAc-6P is generated intracellularly during catabolism of N-acetylneuramic acid (Neu5Ac), a carbon source for C. glutamicum. The sialic acid transport and utilization genes are clustered in C. glutamicum ATCC 13032 together with a regulator (cg2936) belonging to the GntR-family transcription factor (in the region cg2929-cg2940) [28, 29]. The genome of C. glutamicum ATCC 13032 contains a gene (cg3158, nagA 2 ) annotated to encode a putative β-N-acetylglucosaminidase precursor, whose activity and function has not yet been studied. β-N-acetylglucosaminidases (E.C. 126.96.36.199) are characterized by the ability to cleave off single units of GlcNAc from the non-reducing ends of short oligosaccharides or peptidoglycan. A common N-acetylglucosaminidase of bacteria is NagZ, a GH3 family hydrolase that participates in bacterial cell wall recycling . In the Gram-negative model organism E. coli, NagZ is located in the cytoplasm and removes GlcNAc from 1,6-anhydroMurNAc-peptides, that derive from the breakdown of the murein envelope by transglycosylases and endopeptidases during growth . In many Gram-negative bacteria, cell wall recycling is also linked to antibiotic response: the organisms can detect β-lactam antibiotics affecting cell wall formation by sensing an increase in intracellular muropeptides concentration, eliciting resistance mechanisms .
Whether Gram-positive bacteria recycle their cell wall has been questioned for a long time, but recently putative cell recycling pathways have been elucidated for Bacillus subtilis and Clostridium acetobutylicum [32, 33]. In contrast to E. coli and C. acetobutylicum, muropeptide cleavage occurs extracellularly in B. subtilis, whose NagZ is secreted to the medium, where it hydrolyzes GlcNAc from muropeptide units. The released products are transported into the cytoplasm by specific phosphotransferases and further processed for recycling or energy production . In each generation, up to 50 % of peptidoglycan is turned over in B. subtilis and E. coli , due to their cell division mechanism, characterized by intercalation of new cell wall along most of their length. To our knowledge, it is not known whether C. glutamicum recycles peptidoglycan, given that the apical cell elongation occurring during its cell division may not require a massive cell wall breakdown [36, 37]. C. glutamicum lacks orthologs of most of the genes responsible for peptidoglycan recycling as present in E. coli . Moreover, since C. glutamicum does not possess a specific transporter for GlcNAc, it cannot import and utilize GlcNAc as carbon source unless a gene for GlcNAc transport is expressed heterologously .
N-acetylglucosaminidase also catalyzes the last step of bacterial chitin degradation. Many chitinolytic organisms produce a cocktail of chitinases that hydrolyze chitin to the disaccharide N,N'-diacetyl chitobiose as major end product, which is eventually cleaved by a chitobiosidase to GlcNAc monomers . GlcNAc production by enzymatic treatment using specific enzymes from chitinolytic organisms and unspecific crude enzymes such as lysozyme, papaine or lipase is seen more favorably than chemical hydrolysis, since enzymatic hydrolysis of chitin operates under mild conditions without generating large amounts of wastes, an environmental concern of the chemical method [24, 39].
Here we report that C. glutamicum possesses N-acetylglucosaminidase activity and that the respective enzyme is encoded by cg3158/nagA 2 . Its activity was compared to that of the already characterized NagZ from B. subtilis. Furthermore, variants of NagA2, fused to different tags for secretion or without secretion signal, were constructed and tested. The variants with higher secreted activity have been combined in a plasmid with a secreted chitinase, ChiB, from the chitinolytic Gram-negative Serratia marcescens, in order to test if the combination of chitinase and N-acetylglucosaminidase activity supports the growth of C. glutamicum with chitin as carbon source.
C. glutamicum ATCC13032 possesses a GH3 N-acetylglucosaminidase
Overexpression of nagA 2 fused with homologous N-terminal signal peptides increased extracellular activity of the enzyme
Protein coding sequences exhibiting Tat-type signal sequences in C. glutamicum
Protein ID, locus tag
trehalose corynomycolyl transferase
Both, the overall activity of NagA2 and its secretion efficiency, greatly improved upon replacing the native secretion signal peptide. N-acetylglucosaminidase activity in the supernatant ranged from about 85 % to 93 % of the total activity measured inside the cells and in the broth. The signal peptides SP0955, PhoD and Cmt4 led to the highest enzyme activities in the culture broth (corresponding to 0.29 ± 0.05 U, 0.31 ± 0.02 U and 0.34 ± 0.04 U, respectively).
Thus, about sixty fold more N-acetylglucosaminidase activity as compared to the native enzyme was found in the supernatant (Fig. 1). Signal peptides are cleaved during protein export and are expected not to affect kinetic parameters of the mature enzymes. Indeed, the Km values of NagA2 lacking its own signal peptide (0.266 ± 0.037 mM) or fused to the C. glutamicum PhoD signal peptide (0.270 ± 0.066 mM) were shown to be comparable.
B. subtilis NagZ was also expressed with various signal peptides in C. glutamicum (Fig. 1). However, fusion to endogenous signal peptides did not increase the amount of enzyme activity retrieved in the supernatant. Notably, fusion to the signal peptide from Cg0955 increased intracellular enzyme activity, whereas fusion to signal peptide Cmt4 led to reduced intracellular N-acetylglucosaminidase activity (Fig. 1).
Overexpression of ChiB fused with a secretion signal peptide from C. glutamicum enabled secretion of the enzyme
Chitinase activity in the cellular and extracellular fraction of C. glutamicum ΔnanR strain overexpressing chiB from S. marcescens in its native form or fused with the signal peptide from Cg0955
sp. act. (mU/mg)
total activity (U)
total activity (U)
ΔnanR (pVWEx1-nagE) (pEKEx3)
0.5 ± 0.1
0.14 ± 0.05
3.8 ± 0.3
0.17 ± 0.01
0.22 ± 0.03
Concomitant overproduction of ChiB and NagA2 is not sufficient to support growth with colloidal chitin in a C. glutamicum strain able to grow with GlcNAc
Chitinase and β-N-acetylglucosaminidase activities in the strain overexpressing SP0955-chiB and nagA 2 variants with different secretion tags
C. glutamicum strain
ΔnanR (pVWEx1-nagE) carrying vector
sp. act. (mU/mg)
Total activity (U)
Total activity (U)
sp. act. (mU/mg)
Total activity (U)
Total activity (U)
8.7 ± 0.7
0.22 ± 0.01
0.22 ± 0.03
2.1 ± 0.2
0.05 ± 0.01
0.17 ± 0.02
8.1 ± 0.8
0.20 ± 0.01
0.21 ± 0.01
2.4 ± 0.3
0.06 ± 0.01
0.08 ± 0.01
pEKEx3- SP0955-chiB-Cmt4-nagA 2
7.3 ± 0.3
0.16 ± 0.01
0.13 ± 0.01
0.7 ± 0.1
0.02 ± 0.01
0.11 ± 0.01
Here, C. glutamicum was shown to possess N-acetylglucosaminidase activity that is encoded by cg3158/nagA 2 . Structurally, the NagA2 protein belongs to the family 3 glycoside hydrolases, and among these, the N-acetyl-β-D-glucosaminidases show a selective specificity for GlcNAc as substrate  with only few exceptions . N-acetyl-β-D-glucosaminidase activity was assayed with 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside as substrate, and about 0.27 mM supported half-maximal activity. In comparison, NagZ from E. coli had a higher Km on the same substrate (0.43 mM) , whereas NagZ of B. subtilis showed an about two fold lower Km of 0.11 ± 0.0 mM with 4'-methylumbelliferyl-β-GlcNAc as substrate .
The role of the NagA2 activity in C. glutamicum is still unclear. Analysis of the C. glutamicum transcriptome revealed that the nagA2 gene is transcribed as leaderless transcript with a relatively low RNA abundance . It is not known whether nagA2 expression is regulated in C. glutamicum. The adjacent gene cg3157 encodes a hitherto uncharacterized protein with VanW (pfam04294) and peptidoglycan binding domains (pfam12229) that may play a role in cell wall biosynthesis. In contrast, the orthologous nagZ is part of operon in E. coli (hinT-ycfL-lpoB-thiK-nagZ-ycfP) and B. subtilis (ybbIHFEDC). NagZ from B. subtilis and E. coli have been shown to play important roles in cell wall recycling [30, 32, 50]. C. glutamicum lacks orthologs for most of the genes responsible for muropeptide recycling in B. subtilis and orthologs for E. coli genes necessary for import and catabolism of anhydromuropeptides. Therefore, further studies need to be performed in order to elucidate whether C. glutamicum has a peptidoglycan recycling mechanism and if NagA2 is involved.
The endogenous protein NagA2 was shown to be secreted, although inefficiently. Increased protein levels due to changing the nagA 2 translational start codon from GTG to the more common ATG increased total activity. But even though total activity doubled, only around 10 % was found in the supernatant, showing that the endogenous NagA2 signal peptide supports secretion rather inefficiently.
Protein secretion has been studied for decades in C. glutamicum and recently a strain exhibiting potential as host for industrial-scale production of recombinant proteins has been commercialized as Corynex™. C. glutamicum is a favorable host for protein production and secretion, i.e. because it lacks extracellular proteolytic activities . However, shortcomings such as its mycolic acid layer that in combination with the underlying peptidoglycan-arabinogalactan layer constitutes a second permeability barrier [52, 53] or an additional S-layer (present only in some strains) can hamper its utilization for protein production . The strains used in this study are based on ATCC 13032, which lacks an S-layer . C. glutamicum is not widely used for protein production, which may in part be due to variable yields depending on the target protein and on the target signal [42, 54–56].
The promoter and signal sequence of the cspB gene encoding PS2, the major protein secreted by the organism, have been used in many studies of heterologous protein secretion in C. glutamicum [43, 44, 57, 58]. A systematic screen of signal peptides for secretion of α-amylase from Geobacillus stearothermophilus in C. glutamicum showed up to 150-fold better secretion using signal peptides from e.g. genes cg0955, cmt1 and cmt4, and phoD when compared to fusions with the PS2 secretion signal . Conversely, secretion of endogenous NagA2 as well as heterologous ChiB fused to these signal peptides did not differ much in terms of overall secreted activity, utilizing different secretion signals (Fig. 1). Notably, signal peptides involved in the Sec pathway, (PS2, Cmt1 and Cmt4) or the Tat pathway (Cg0955 and PhoD) functioned similarly with NagA2 and ChiB (Fig. 1), whereas for other proteins, e.g. GFP, the translocation efficiency and/or final protein activity was strongly affected by the route of transport [59–61].
Despite of detection of GlcNAc units in culture supernatant, secretion of ChiB and NagA2 by C. glutamicum strain ΔnanR (pVWEx1-nagE) (pEKEx3-SP0955-chiB-SP0955-nagA 2 ) was too low to support growth on colloidal chitin. Extracellular activities were never higher than 5 mU mL−1 (Fig. 1). This contrasts to recombinant C. glutamicum strains which are able to grow with starch as sole carbon source due to secretion of heterologous α-amylases [15, 57] due to about 100 fold higher α-amylase activities (400 to 650 mU mL−1).
A strategy for the hydrolysis of complex bio-polymers, alternative to the secretion of degrading enzymes, is their display on the cell surface, anchored to membrane integral proteins. One notable example for this approach is the heterologous expression of the α-amylase from Streptococcus bovis 148 (amyA), that allowed the growth with starch as carbon source when fused to C. glutamicum anchor proteins such as porins , the glutamate exporter NCgl1221 , or heterologous proteins such as PgsA from B. subtilis . More recently, it was shown that the combined surface display in C glutamicum of endoglucanase  and β-glucosidase (BglA) from Clostridium thermocellum, anchored to the mechanosensitive channel Msc, enabled the saccharification of lignocellulosic material, leading to up to 6-fold increased reducing sugar generation when compared to secreted cellulases . It remains to be established if surface display of N-acetylglucosaminidases and chitinases is superior to secretion of these enzymes. It may also be necessary to simultaneously overproduce chitinases with different mechanisms of action, e.g. combining an endo-chitinase, e.g. ChiC from S. marcescens, with a processive chitinase, e.g. ChiB from S. marcescens [24, 66, 67].
C. glutamicum possesses β-N-acetylglucosaminidase. In the wild type, β-N-acetylglucosaminidase activity was too low to be detected. However, overproduction of the enzyme fused to TAT or Sec signal peptides led to secretion of active β-N-acetylglucosaminidase. The engineering of the signal peptide improved the secretion more than 60 folds as compared to the native sequence. NagZ of B. subtilis could be overproduced, but was not excreted efficiently, even when fused to C. glutamicum TAT or Sec signal peptides. Concomitant secretion of exochitinase ChiB from S. marcescens and endogenous NagA2 did not result in growth with colloidal chitin as sole or combined carbon source. This may indicate that either higher enzyme activities are needed or that additional enzymes such as processive chitinase or endochitinase activities may have to be overproduced.
Microorganisms, media, and cultivation conditions
Bacterial strains and plasmids used in this study
Strain or plasmid
F− thi −1 endA1 hsdr17 (r−, m−) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 relA1
Wild-type strain ATCC 13032, auxotrophic for biotin
cg2936 deletion mutant of C. glutamicum WT
(Matano & Wendisch, unpublished)
KanR; C. glutamicum/E. coli shuttle vector (Ptac, lacIq; pHM1519, OriVE.c.)
pVWEx1 carrying nagE (full gene) from C. glycinophilum ATCC 21341, with mutation (ATG start codon) and optimized RBS
SpecR; C. glutamicum/E. coli shuttle vector (Ptac, lacIq; pBL1, OriVE.c.)
pEKEx3carrying chiB gene from S.marcescens ATCC13880
pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955
pEKEx3carrying nagA2 gene from C. glutamicum WT
pEKEx3carrying nagA2 gene from C. glutamicum WT, with mutation (ATG codon)
pEKEx3- SP-less-nagA 2
pEKEx3carrying nagA2 gene from C. glutamicum WT, without its own putative signal sequence.
pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli
pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli., with signal sequence from cg0955
pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from PS2 (cspB) C. glutamicum ATCC 14067
pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from phod
pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from cmt4
pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from cmt1
pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cg0955
pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from PS2 (cspB) C. glutamicum ATCC 14067
pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from phoD
pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cmt4
pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cmt1
pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from cg0955
pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from phoD
pEKEx3- SP0955-chiB-Cmt4-nagA 2
pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from cmt4
Preparation of colloidal chitin
A modified  method was used for colloidal chitin preparation. 5 g of chitin (Sigma) were added to 50 mL of concentrated hydrochloric acid. The mixture was incubated overnight at 4 °C while continuously stirring. Chitin was precipitated as colloidal suspension by the slow addition of deionized water and the chitin suspension was washed with deionized water until a neutral pH value was reached. The colloidal chitin obtained was resuspended in medium for growth assay in a final concentration of 10 g L−1.
DNA preparation, manipulation and transformation
Plasmids were constructed in E. coli DH5α from PCR-generated fragments (KOD Hot Start DNA Polymerase; Novagen), using C. glutamicum ATCC 13032, S. marcescens ATCC 13880 genomic DNA as template. C. glutamicum genomic DNA was prepared as described ; S. marcescens ATCC 13880 (DSM 30121) genomic DNA was obtained by DSMZ German Collection of Microorganism and Cell Cultures (Braunschweig, Germany). A nagZ gene (GenBank Acc. No. CAB11942.1) from B. subtilis 168 (ATCC 23857) was synthesized in a codon optimized version for overexpression in E. coli (Genscript, USA). The oligonucleotides used in this study are listed in (Additional file 1: Table S1). E. coli was transformed by standard methods  and the plasmids were introduced by electroporation into C. glutamicum strains as described . Transformants containing two compatible plasmids, here pVWEx1 and pEKEx3 and their derivatives, were selected by plating on agar plates containing kanamycin (25 μg/ml) and spectinomycin (100 μg/ml). Vector maps of pVWEx1-nagE and pEKEx3-SP0955-chiB-SP0955-nagA2 are shown in Additional file 1: Figure S1. Transformants were analysed by PCR with appropriate primers; the absence of mutations in the cloned genes was verified by sequencing.
Construction of plasmids and strains
For the construction of plasmids expressing chiB, nagZ and nagA 2 , with their own signal peptide or in fusion with C. glutamicum signal peptides, KOD PCR products were ligated into BamHI, EcoRI digested pEKEx3 via Gibson assembly . The chiB gene was cloned into pEKEx3 in its native form and in fusion with a signal peptide from gene cg0955 of C. glutamicum ATCC 13032 (hence SP0955). The gene nagA 2 has been cloned in its native form, including its own signal peptide, with a mutation on the first triplet replacing the original GTG start codon with the ATG triplet, without its own putative signal sequence (hence SP-less- nagA 2 ) and with its signal peptide replaced by secretion sequences from C. glutamicum, here named SP0955, PS2, PhoD, Cmt4 and Cmt1.
B. subtilis nagZ has been cloned with its own signal peptide or in a signal peptide-less form, in fusion with the above mentioned signal peptides SP0955, PS2, PhoD, Cmt4 and Cmt1.
Glycol chitin preparation and dot blot chitinase assay
Glycol chitin was prepared by re-acetylation of glycol chitosan. 1 g of glycol chitosan (Sigma-Aldrich) was added to 20 mL of 10 % acetic acid and the mixture was incubated overnight while continuously stirring. 90 mL of methanol were slowly added and the solution was vacuum filtered on Buchner funnel. 1.5 mL of acetic anhydride were added to the filtrate and the solution was incubated 30 min at room temperature allowing the formation of a solid gel. The gel was cut into small pieces and homogenized in a waring blender. The homogenized gel was centrifuged and washed with methanol and resuspended a last time in 100 ml H2O to give a ca 1 % (w/v) solution of glycol chitin.
A dot blot assay on 12 % (v/v) acrylamide gel, with 50 mM TEA buffer (pH 7.0), containing 0.01 % (v/v) of glycol chitin was used for chitinolytic activity determination. Fractions of cell extract or supernatant from C. glutamicum WT (pEKEx3-chiB), C. glutamicum WT (pEKEx3) and ΔnanR (pEKEx3 SP0955-chiB) were spotted on the gel. After overnight incubation the gel was stained for 30 minutes with 0.01 % (w/v) Calcofluor White M2R (Sigma Aldrich, Germany) solution, destained and visualized by UV illumination . Chitinolytic activity is revealed as dark halo on fluorescent background.
Chitinase and N-acetylglucosaminidase activity assays
Aliquots from C. glutamicum BHI cultures were withdrawn during the exponential growth phase and cells where harvested by centrifugation (10 min, 3,200 x g and 4 °C). Supernatant and cell extract fractions from the aliquots were used for chitinase and N-acetylglucosaminidase activity quantification. Cells were disrupted by ultrasonic treatment (UP 200S; Dr. Hielscher GmbH, Teltow, Germany) with an amplitude of 50 % and a duty circle of 0.5 for 7 min, upon resuspension in 100 mM phosphate buffer (pH 7.0). The cell suspension was centrifuged for 1 h at 4 °C and 16,000 rpm and the soluble cell extract was recovered. Exo-chitinase and N-acetylglucosaminidase activity were determined by measuring the hydrolysis of p-nitrophenol from 4-Nitrophenyl N,N’-diacetyl-β-D-chitobioside and 4-Nitrophenyl N-acetyl-β-D-glucosaminide (Sigma Aldrich, Germany), respectively. The substrates were dissolved in 100 mM phosphate buffer (pH 7.0) solution at a concentration of 1 mg mL−1 and the activity was determined, following the chitinases assay protocol from Sigma Aldrich in 96 well plates, measuring the absorption at 405 nm of hydrolyzed p-nitrophenol upon addition of sodium carbonate stop solution. Km values have been estimated with the Eadie Hofstee plot for NagA2 without its own signal peptide and as fusion protein with PhoD signal peptide, measuring the activity with different concentration of 4-Nitrophenyl N,N’-diacetyl-β-D-chitobioside in the cell extract fraction and in the supernatant, respectively.
Quantification of GlcNAc via DNS colorimetric assay
GlcNAc released in the supernatant was determined spectrophotometrically by the Dinitrosalicylic acid (DNS) method following the protocol described by Miller  at 540 nm using 3 ml of culture supernatant. Concentrations of the released sugars were estimated using a standard calibration curve of GlcNAc (Sigma Aldrich, Germany) in a range of 0–2 mM.
Work by CM and VFW was funded in part by grants 0315589G from BMBF in the CRP “Corynebacterium: improving flexibility and fitness for industrial production”. We acknowledge support of the publication fee by Deutsche Forschungsgemeinschaft and the Open Access Publication Funds of Bielefeld University.
Availability of data and material
All data are provided in the main manuscript and the supplementary files.
VFW, BM and CM designed the experiments, reviewed and revised the manuscript. SK and SH supplied the nagZ gene from B. subtilis 168 used in the study and provided support with the analytical methods for determining the activity of chitin degrading enzymes. ES planned and conduct some experiments. CM conducted the experiments, analyzed the results and drafted the manuscript. VFW coordinated the study and helped finalize the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
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