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Table 4 Chitinase and β-N-acetylglucosaminidase activities in the strain overexpressing SP0955-chiB and nagA 2 variants with different secretion tags

From: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase

C. glutamicum strain ChiB activity NagA2 activity
Intracellular Extracellular Intracellular Extracellular
ΔnanR (pVWEx1-nagE) carrying vector sp. act. (mU/mg) Total activity (U) Total activity (U) sp. act. (mU/mg) Total activity (U) Total activity (U)
pEKEx3-SP0955-chiB-SP0955-nagA 2 8.7 ± 0.7 0.22 ± 0.01 0.22 ± 0.03 2.1 ± 0.2 0.05 ± 0.01 0.17 ± 0.02
pEKEx3-SP0955-chiB-PhoD-nagA 2 8.1 ± 0.8 0.20 ± 0.01 0.21 ± 0.01 2.4 ± 0.3 0.06 ± 0.01 0.08 ± 0.01
pEKEx3- SP0955-chiB-Cmt4-nagA 2 7.3 ± 0.3 0.16 ± 0.01 0.13 ± 0.01 0.7 ± 0.1 0.02 ± 0.01 0.11 ± 0.01
  1. Data represent means and SD from activity assays conducted on three independent cultivations