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Table 4 Chitinase and β-N-acetylglucosaminidase activities in the strain overexpressing SP0955-chiB and nagA 2 variants with different secretion tags

From: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase

C. glutamicum strain

ChiB activity

NagA2 activity

Intracellular

Extracellular

Intracellular

Extracellular

ΔnanR (pVWEx1-nagE) carrying vector

sp. act. (mU/mg)

Total activity (U)

Total activity (U)

sp. act. (mU/mg)

Total activity (U)

Total activity (U)

pEKEx3-SP0955-chiB-SP0955-nagA 2

8.7 ± 0.7

0.22 ± 0.01

0.22 ± 0.03

2.1 ± 0.2

0.05 ± 0.01

0.17 ± 0.02

pEKEx3-SP0955-chiB-PhoD-nagA 2

8.1 ± 0.8

0.20 ± 0.01

0.21 ± 0.01

2.4 ± 0.3

0.06 ± 0.01

0.08 ± 0.01

pEKEx3- SP0955-chiB-Cmt4-nagA 2

7.3 ± 0.3

0.16 ± 0.01

0.13 ± 0.01

0.7 ± 0.1

0.02 ± 0.01

0.11 ± 0.01

  1. Data represent means and SD from activity assays conducted on three independent cultivations