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Table 5 Bacterial strains and plasmids used in this study

From: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase

Strain or plasmid Relevant characteristics Reference
E.coli   
DH5α F thi 1 endA1 hsdr17 (r, m) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 relA1 [75]
C. glutamicum   
WT Wild-type strain ATCC 13032, auxotrophic for biotin ATCC
ΔnanR cg2936 deletion mutant of C. glutamicum WT (Matano & Wendisch, unpublished)
Plasmids   
pVWEx1 KanR; C. glutamicum/E. coli shuttle vector (Ptac, lacIq; pHM1519, OriVE.c.) [76]
pVWEx1-nagE pVWEx1 carrying nagE (full gene) from C. glycinophilum ATCC 21341, with mutation (ATG start codon) and optimized RBS [22]
pEKEx3 SpecR; C. glutamicum/E. coli shuttle vector (Ptac, lacIq; pBL1, OriVE.c.) [3]
pEKEx3-chiB pEKEx3carrying chiB gene from S.marcescens ATCC13880 This work
pEKEx3-SP0955-chiB pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 This work
pEKEx3-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT This work
pEKEx3-ATG-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, with mutation (ATG codon) This work
pEKEx3- SP-less-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, without its own putative signal sequence. This work
pEKEx3-nagZ pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli This work
pEKEx3-SP0955-nagZ pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli., with signal sequence from cg0955 This work
pEKEx3-PS2-nagZ pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from PS2 (cspB) C. glutamicum ATCC 14067 This work
pEKEx3-PhoD-nagZ pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from phod This work
pEKEx3-Cmt4-nagZ pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from cmt4 This work
pEKEx3-Cmt1-nagZ pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from cmt1 This work
pEKEx3-SP0955-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cg0955 This work
pEKEx3-PS2-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from PS2 (cspB) C. glutamicum ATCC 14067 This work
pEKEx3-PhoD-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from phoD This work
pEKEx3-Cmt4-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cmt4 This work
pEKEx3-Cmt1-nagA 2 pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cmt1 This work
pEKEx3-SP0955-chiB-SP0955nagA 2 pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from cg0955 This work
pEKEx3-SP0955-chiB-PhoD-nagA 2 pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from phoD This work
pEKEx3- SP0955-chiB-Cmt4-nagA 2 pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from cmt4 This work