Table 5 Bacterial strains and plasmids used in this study
From: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase
Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
E.coli | ||
DH5α | F− thi −1 endA1 hsdr17 (r−, m−) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 relA1 | [75] |
C. glutamicum | ||
WT | Wild-type strain ATCC 13032, auxotrophic for biotin | ATCC |
ΔnanR | cg2936 deletion mutant of C. glutamicum WT | (Matano & Wendisch, unpublished) |
Plasmids | ||
pVWEx1 | KanR; C. glutamicum/E. coli shuttle vector (Ptac, lacIq; pHM1519, OriVE.c.) | [76] |
pVWEx1-nagE | pVWEx1 carrying nagE (full gene) from C. glycinophilum ATCC 21341, with mutation (ATG start codon) and optimized RBS | [22] |
pEKEx3 | SpecR; C. glutamicum/E. coli shuttle vector (Ptac, lacIq; pBL1, OriVE.c.) | [3] |
pEKEx3-chiB | pEKEx3carrying chiB gene from S.marcescens ATCC13880 | This work |
pEKEx3-SP0955-chiB | pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 | This work |
pEKEx3-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT | This work |
pEKEx3-ATG-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, with mutation (ATG codon) | This work |
pEKEx3- SP-less-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, without its own putative signal sequence. | This work |
pEKEx3-nagZ | pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli | This work |
pEKEx3-SP0955-nagZ | pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli., with signal sequence from cg0955 | This work |
pEKEx3-PS2-nagZ | pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from PS2 (cspB) C. glutamicum ATCC 14067 | This work |
pEKEx3-PhoD-nagZ | pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from phod | This work |
pEKEx3-Cmt4-nagZ | pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from cmt4 | This work |
pEKEx3-Cmt1-nagZ | pEKEx3carrying nagZ gene from B.subtilis 168, codon optimized for expression in E.coli, with signal sequence from cmt1 | This work |
pEKEx3-SP0955-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cg0955 | This work |
pEKEx3-PS2-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from PS2 (cspB) C. glutamicum ATCC 14067 | This work |
pEKEx3-PhoD-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from phoD | This work |
pEKEx3-Cmt4-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cmt4 | This work |
pEKEx3-Cmt1-nagA 2 | pEKEx3carrying nagA2 gene from C. glutamicum WT, with signal sequence from cmt1 | This work |
pEKEx3-SP0955-chiB-SP0955nagA 2 | pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from cg0955 | This work |
pEKEx3-SP0955-chiB-PhoD-nagA 2 | pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from phoD | This work |
pEKEx3- SP0955-chiB-Cmt4-nagA 2 | pEKEx3carrying chiB gene from S.marcescens ATCC13880, with signal sequence from cg0955 and nagA2 gene from C. glutamicum WT, with signal sequence from cmt4 | This work |