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Table 3 Chitinase activity in the cellular and extracellular fraction of C. glutamicum ΔnanR strain overexpressing chiB from S. marcescens in its native form or fused with the signal peptide from Cg0955

From: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase

 

Chitinase activity

 

Intracellular

Extracellular

Strain

sp. act. (mU/mg)

total activity (U)

total activity (U)

ΔnanR (pVWEx1-nagE) (pEKEx3)

<0.01

<0.001

<0.01

ΔnanR (pEKEx3-chiB)

0.5 ± 0.1

0.14 ± 0.05

<0.01

ΔnanR (pEKEx3-SP0955-chiB)

3.8 ± 0.3

0.17 ± 0.01

0.22 ± 0.03

  1. Data represent means and SD from activity assays conducted on three independent cultivations