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Table 3 Chitinase activity in the cellular and extracellular fraction of C. glutamicum ΔnanR strain overexpressing chiB from S. marcescens in its native form or fused with the signal peptide from Cg0955

From: Corynebacterium glutamicum possesses β-N-acetylglucosaminidase

  Chitinase activity
  Intracellular Extracellular
Strain sp. act. (mU/mg) total activity (U) total activity (U)
ΔnanR (pVWEx1-nagE) (pEKEx3) <0.01 <0.001 <0.01
ΔnanR (pEKEx3-chiB) 0.5 ± 0.1 0.14 ± 0.05 <0.01
ΔnanR (pEKEx3-SP0955-chiB) 3.8 ± 0.3 0.17 ± 0.01 0.22 ± 0.03
  1. Data represent means and SD from activity assays conducted on three independent cultivations