The gene for a lectin-like protein is transcriptionally activated during sexual development, but is not essential for fruiting body formation in the filamentous fungus Sordaria macrospora
© Nowrousian and Cebula; licensee BioMed Central Ltd. 2005
Received: 10 September 2005
Accepted: 03 November 2005
Published: 03 November 2005
The filamentous fungus Sordaria macrospora forms complex three-dimensional fruiting bodies called perithecia that protect the developing ascospores and ensure their proper discharge. In previous microarray analyses, several genes have been identified that are downregulated in sterile mutants compared to the wild type. Among these genes was tap1 (t ranscript a ssociated with p erithecial development), a gene encoding a putative lectin homolog.
Analysis of tap1 transcript levels in the wild type under conditions allowing only vegetative growth compared to conditions that lead to fruiting body development showed that tap1 is not only downregulated in developmental mutants but is also upregulated in the wild type during fruiting body development. We have cloned and sequenced a 3.2 kb fragment of genomic DNA containing the tap1 open reading frame and adjoining sequences. The genomic region comprising tap1 is syntenic to its homologous region in the closely related filamentous fungus Neurospora crassa. To determine whether tap1 is involved in fruiting body development in S. macrospora, a knockout construct was generated in which the tap1 open reading frame was replaced by the hygromycin B resistance gene hph under the control of fungal regulatory regions. Transformation of the S. macrospora wild type with this construct resulted in a tap1 deletion strain where tap1 had been replaced by the hph cassette. The knockout strain displayed no phenotypic differences under conditions of vegetative growth and sexual development when compared to the wild type. Double mutants carrying the Δtap1 allele in several developmental mutant backgrounds were phenotypically similar to the corresponding developmental mutant strains.
The tap1 transcript is strongly upregulated during sexual development in S. macrospora; however, analysis of a tap1 knockout strain shows that tap1 is not essential for fruiting body formation in S. macrospora.
Fruiting body formation in ascomycetes is a complex process leading to the formation of a number of specialized cell types from a comparatively undifferentiated vegetative mycelium . Recently, the molecular basis of this process has been investigated by forward and reverse genetics approaches, and a number of genes that are essential for fruiting body development have been identified. However, a coherent picture of fungal multicellular development has yet to emerge .
One avenue towards a deeper understanding of developmental processes is by functional genomics analyses, e.g. microarray studies. Such approaches can help to identify genes that are regulated differentially during fruiting body development and are therefore candidates for further functional analysis. In a previous study, we have performed microarray analyses of fruiting body development in S. macrospora . This ascomycete is homothallic and produces fruiting bodies called perithecia within seven days under laboratory conditions. S. macrospora is a close relative of N. crassa, but in contrast to N. crassa, it does not produce any asexual spores. Therefore, changes of gene expression patterns during sexual development are not superimposed by changes related to asexual sporulation. We have previously analyzed gene expression in three developmental mutants of S. macrospora, and have identified a number of genes that are downregulated in the sterile mutants when compared with the wild type . One of these genes is tap1 (t ranscript a ssociated with p erithecial development, formerly known as SMU5651 ). tap1 transcript levels are downregulated in the three developmental mutants pro1, pro11 and pro22 as well as in all three double mutants which led us to speculate that the gene might be involved in sexual development in S. macrospora. In addition to this intriguing expression pattern, the derived TAP1 amino acid sequence shows homology to lectins from other filamentous fungi with the highest similarity to lectins isolated from fruiting bodies of several basidiomycetes . It has long been speculated that lectins play a role in fungal development; however, as no mutants in lectin-encoding genes from fruiting body-producing fungi have been analyzed to date, definite proof for this hypothesis is lacking [4, 5]. The only known fungal lectin mutant is a strain of Arthrobotrys oligospora in which the lectin gene aol was deleted . This mutant does not exhibit any phenotypical differences from the wild type under all conditions investigated, but as no sexual cycle is known for A. oligospora, the question whether aol might be involved in fruiting body development could not be addressed. Here, we present data on the expression of the S. macrospora tap1 gene as well as the characterization of a tap1 knockout strain to address the role of a putative lectin in fungal sexual development.
Expression of tap1 during sexual development in S. macrospora
Sequence analysis of the S. macrospora tap1 gene
Construction of a Δtap1 strain
Phenotypic characterization of the Δtap1 strain in different genetic backgrounds
We then investigated whether there were any phenotypes unrelated to sexual development present in the mutant strains. Mycelial growth rates were determined as 24.7 (± 1.7) and 24.8 (± 1.4) mm per day for Δtap1 and wild type respectively; thus, indicating that mycelial growth is not altered in the knockout strains. Also, renewal of growth and fruiting body development after incubation at 4°C or 37°C, conditions that prevent growth and fruiting body formation, respectively, was not different in the mutants when compared to the wild type (data not shown). Also, wettability of mycelium and fruiting bodies was similar in wild type and mutants indicating that the hydrophobic coating of the mycelium was not altered in the knockout strains. As the lack of tap1 did not cause any discernable phenotype, we wondered whether tap1 might have a redundant function or whether its absence might be masked by the presence of other genes. We therefore crossed the Δtap1 allele into strains bearing mutations in other developmental genes, namely pro1, pro11, pro22, and pro41. The pro1 mutant is defective in a gene encoding a transcription factor , the pro11 mutant lacks a functional WD40 repeat protein , and pro22 and pro41 are mutants non-allelic to tap1 or the other pro genes (Kück et al., unpublished data). tap1 transcript levels are downregulated in mutants pro1, pro11, pro22 , and pro41 (Nowrousian, unpublished data). Thus, we speculated whether a complete lack of tap1 would lead to a more pronounced developmental phenotype, especially as several other developmental genes are also downregulated in the pro mutants . We obtained double mutants with strains pro1, pro11, pro22, and pro41 and verified the presence of the Δtap1 allele in the double mutants by Southern blot analysis (Figure 5). All double mutant strains were phenotypically similar to the respective single pro mutants in that they produced only protoperithecia and were therefore sterile. There were no differences in the number of protoperithecia produced by the double mutants when compared with the single mutants. Also, growth rates of the vegetative mycelium as well as overall morphological appearance were the same (data not shown). For the crosses of the tap1 knockout with the pro mutant strains and subsequent back-crosses, we analyzed a total of 17 full tetrads and 37 partial tetrads from crosses with different mutant strains and in all cases found the expected 1:1 segregation pattern for each of the single markers (hygromycin resistance and pro mutant phenotype, respectively). This is a further indication that deletion of tap1 does not interfere with sexual development, and also shows that the process of generating the Δtap1 allele did not introduce further mutations into the strains that would cause any different segregation patterns. Overall, no phenotype for Δtap1 was found in any of the genetic backgrounds investigated.
The tap1-encoded polypeptide from S. macrospora has significant homology to lectins and lectin-like protein from other fungi (Figure 3). The highest degree of amino acid identity is found in comparison with lectins that were isolated from fruiting bodies of several basidiomycetes [12–14]. Lectins are carbohydrate-binding proteins that are found in a variety of organisms [4, 5]. On the basis of sequence homology, the S. macrospora TAP1 polypeptide can be included into a class of fungal lectins; however, whether it has lectin activity, i.e. whether it specifically binds carbohydrates, remains to be elucidated. Interestingly, TAP1 displays a greater sequence identity towards basidiomycete lectins than to lectins or putative lectins from ascomycetes with the (notable) exception of its closest homolog, the N. crassa protein NCU05651.2 (Figure 3). However, BLAST searches in the N. crassa genome  with the sequences of TAP1 as well as the lectin sequences from the other fungi used in our sequence comparisons yielded only NCU05651.2 as a significant result (data not shown). This finding indicates that the gene is present as a single copy in N. crassa, and that there is no other member of this lectin gene family present in the N. crassa genome. As N. crassa and S. macrospora are close relatives with highly syntenic genomes , it is likely that this is the case in S. macrospora as well. This observation is supported by the fact that only a single band in S. macrospora genomic DNA hybridizes with a tap1 probe (Figure 5). Thus, it seems that this particular class of fungal lectins has evolved faster in S. macrospora and N. crassa compared to other ascomycetes, for which it still retains more similarity with its basidiomycete relatives (Figure 3). Another class of fungal lectins has been found in the basidiomycete Coprinus cinereus. These lectins bind galactose and are therefore called galectins, and two galectins from C. cinereus are specifically expressed during different stages of fruiting body formation [16, 17]. However, BLAST searches for galectin homologs in the N. crassa genome yielded no significant results (data not shown) indicating that no galectin-like proteins exist in N. crassa or that their sequences are too dissimilar to the C. cinereus sequences to be detected by sequence comparisons alone. Thus, the N. crassa NCU05651.2 gene and its S. macrospora ortholog tap1 are so far the only genes encoding putative lectins that have been identified by sequence analysis in these two ascomycetes.
In fungi, most lectins have been isolated from basidiomycetes, especially from mushroom fruiting bodies, and it has been speculated that they play a role in fruiting body development [5, 18, 19]. However, as no lectin mutant in a fruiting body-producing fungus has been characterized to date, this hypothesis has not been verified experimentally. Previous investigations and the results presented here show that tap1 transcript levels are closely correlated with fruiting body development in S. macrospora; therefore, we decided to construct a tap1 knockout strain to analyze whether this putative lectin plays a role in fruiting body formation. A Δtap1 strain was generated by gene replacement, but the knockout strain has no discernable phenotype under all conditions investigated. This might indicate that tap1 has indeed no function in vegetative growth or sexual development of S. macrospora; however, the possibility that tap1 is needed under environmental conditions not tested in our experiments or that its function is redundant or that in the absence of tap1, another gene product can take its place, cannot be excluded. The latter effect is well known in other organisms, and it was, for example, tested in a large-scale analysis of yeast synthetic lethal interactions where it was found that genes involved in similar biological processes, but not necessarily in the same regulatory pathway, can buffer one another in single mutant backgrounds but show a phenotype in the double mutant strain . To test whether any of the known developmental genes of S. macrospora show this kind of genetic interaction with tap1, we obtained double mutants of Δtap1 with strains bearing mutations in the developmental genes pro1, pro11, pro22, or pro41. However, all double mutant strains were phenotypically similar to the respective pro mutant strains. tap1 transcript levels are downregulated in the pro mutants, and our analysis demonstrates that even the complete loss of tap1 does not worsen the condition of the mutants. This leaves open the possibility that tap1 is necessary in a different genetic background or under different environmental conditions.
With respect to fruiting body formation, our results show that the putative lectin-encoding gene tap1 is not an essential gene for this developmental process. As mentioned previously, the only other known fungal lectin mutant is the aol mutant of the nematode-trapping fungus A. oligospora . Similar to our findings, the aol mutant also has no phenotype under all conditions investigated. Since no sexual stages from A. oligospora are known, these observations do not include fruiting body formation; however, vegetative growth, conidiation, and nematode-trapping were unchanged in the aol mutant strain . Thus, possible functions of this class of lectins and lectin-like proteins in filamentous fungi remain enigmatic.
tap1 expression is strongly associated with sexual development in S. macrospora. An analysis of the tap1 gene and its surrounding genomic region revealed a high degree of sequence identity and overall synteny with the corresponding region in the genome of N. crassa. Sequence comparisons of TAP1 with lectins and lectin-like proteins from other fungi indicate that it is most closely related to lectins isolated from basidiomycete fruiting bodies. However, analysis of a tap1 knockout strain shows that tap1 is not essential for fruiting body formation nor vegetative growth in S. macrospora. This is the case for a Δtap1 allele in an otherwise wild type genetic background as well as in combination with mutations in several developmental genes. Whether tap1 has any function under growth conditions not investigated here, e.g. in a more natural setting, remains to be elucidated.
Strains and growth conditions
S. macrospora strains used in this study. All strains are single spore isolates and are kept in our laboratory collection. The fus allele that is present in some of the strains is a spore color marker (brown instead of black spores) but has no influence on growth or fertility.
tap1 deletion strain
tap1 deletion strain
sterile mutant 
sterile mutant 
Δtap1, pro11, fus
Δtap1, pro11, fus
Δtap1, pro22, fus
Δtap1, pro22, fus
RNA extraction and quantitative real time PCR
Extraction of total RNA and quantitative real time PCR were performed as described previously  with the following modifications: reverse transcription was performed with 400 U Superscript II (Invitrogen) and 0.33 mM dNTPs, and real time PCR was carried out in a DNA Engine Opticon 2 (MJ Research).
Identification of a cosmid clone carrying tap1 and analysis of the tap1 gene
An indexed S. macrospora cosmid library  was screened for tap1 by PCR with oligonucleotides SMU5651-1 (5' CATCAACGACACCTCCGACACCC) and SMU5651-2 (5' CATCGGCCTGATAGAACTTGATCC). For a first round of screening, pooled DNA from 48 cosmid clones was used as a template, DNA from clones from positive pools was then subpooled and used for the next round of screening. This led to the isolation of cosmid D3 from pool VI518-614 that contains the tap1 gene. A 3.2 kb Bgl II restriction fragment carrying tap1 was subcloned from cosmid D3 into pBluescript II/KS+ (Stratagene). The insert of the resulting vector pPC24 was sequenced at MWG Biotech or GATC Biotech AG [emb:AJ781427.2].
Construction of a Δtap1 strain
To create a tap1 knockout construct for homologous recombination in S. macrospora, flanking regions upstream and downstream of the tap1 open reading frame were amplified by PCR from S. macrospora genomic DNA using oligonucleotides SMU5651-BamHI (5' AGGATCCGTGATTCTCATGCTGTGGAAGGAAGC) and SMU5651-NheI (5' AGCTAGCTTTGGCGGTTTGGTTGGGGGGTTGGT) for the upstream region and oligonucleotides SMU5651-ApaI (5' AGGGCCCGTACTCGTCAGTGGGAAAGTGGGTGG) and SMU5651-SacI (5' AGAGCTCTATGCACTTGCTCCTCAAGCGTCTC) for the downstream region, introducing restriction sites as indicated in the oligonucleotide names. Additionally, the hygromycin-resistance cassette consisting of the hph gene from Escherichia coli and the trpC promoter from Aspergillus nidulans was amplified from plasmid pCB1004  using oligonucleotides Hph-ApaI (5' AGGGCCCTCAACGGAACCCTATTCCTTTGCCC) and Hph-NheI (5' AGCTAGCAACTGATATTGAAGGAGCATTTTTGG). All PCR fragments were subcloned in pDrive (Qiagen) resulting in pDriveA (upstream region), pDriveB (downstream region) and pDrivehph (hygromycin-resistance cassette). Sequences of inserts and orientation within the vector was verified by restriction analysis and sequencing (MWG Biotech AG). The PCR fragment containing the downstream region was obtained by Apa I/Sac I digestion of pDriveB and cloned into Apa I/Sac I digested vector pDriveA resulting in plasmid pDriveAB. The hygromycin-resistance cassette was obtained by digesting plasmid pDrivehph with Nhe I and Apa I and was cloned into plasmid pDriveAB hydrolyzed with Nhe I and Apa I resulting in the knockout plasmid pABXY. For transformation of S. macrospora, plasmid pABXY was digested with Bam HI and Sac I and the knockout cassette was obtained by gel elution. The knockout cassette was transformed into the S. macrospora wild type and primary transformants were screened for homologous integration by PCR. For this purpose, total DNA was prepared from the transformants according to , and PCR was performed with oligonucleotides d1 (5' CGATGGCTGTGTAGAAGTACTCGC) and d2 (5' TGCCTCCTCCGAGGCTGATAACCT) for the downstream region or d3 (5' CGGTGGGTAAGGTATCTCTGATG) and d4 (5' CACCGCCTGGACGACTAAACCAA) for the upstream region (Figure 4).
The authors would like to thank Swenja Ellßel and Susanne Schlewinski for excellent technical assistance and Prof. Dr. Ulrich Kück for his generous support that enabled us to carry out this study as part of project A1 of research initiative SFB 480 funded by the Deutsche Forschungsgemeinschaft (DFG).
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