Transcript levels of tap1 are upregulated during sexual development. tap1 transcript levels in the wild type under conditions of vegetative and sexual development were analyzed by real time PCR. For normalization, transcript levels of the SSUrRNAs were used as described previously . Expression is given as fold induction (mean of two independent experiments, error bars indicate standard deviation) with transcript levels during vegetative growth set to 1. Real time PCR results were tested for significance of differential expression at a level of p = 0.001 using REST .