Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile
© Zhu et al; licensee BioMed Central Ltd. 2010
Received: 1 May 2010
Accepted: 12 November 2010
Published: 12 November 2010
Staphylococcus epidermidis has emerged as one of the most important nosocomial pathogens, mainly because of its ability to colonize implanted biomaterials by forming a biofilm. Extensive studies are focused on the molecular mechanisms involved in biofilm formation. The LytSR two-component regulatory system regulates autolysis and biofilm formation in Staphylococcus aureus. However, the role of LytSR played in S. epidermidis remained unknown.
In the present study, we demonstrated that lytSR knock-out in S. epidermidis did not alter susceptibility to Triton X-100 induced autolysis. Quantitative murein hydrolase assay indicated that disruption of lytSR in S. epidermidis resulted in decreased activities of extracellular murein hydrolases, although zymogram showed no apparent differences in murein hydrolase patterns between S. epidermidis strain 1457 and its lytSR mutant. Compared to the wild-type counterpart, 1457ΔlytSR produced slightly more biofilm, with significantly decreased dead cells inside. Microarray analysis showed that lytSR mutation affected the transcription of 164 genes (123 genes were upregulated and 41 genes were downregulated). Specifically, genes encoding proteins responsible for protein synthesis, energy metabolism were downregulated, while genes involved in amino acid and nucleotide biosynthesis, amino acid transporters were upregulated. Impaired ability to utilize pyruvate and reduced activity of arginine deiminase was observed in 1457ΔlytSR, which is consistent with the microarray data.
The preliminary results suggest that in S. epidermidis LytSR two-component system regulates extracellular murein hydrolase activity, bacterial cell death and pyruvate utilization. Based on the microarray data, it appears that lytSR inactivation induces a stringent response. In addition, LytSR may indirectly enhance biofilm formation by altering the metabolic status of the bacteria.
Staphylococcus epidermidis is an opportunistic pathogen which normally inhabits human skin and mucous membranes, primarily infecting immunocompromised individuals or those with implanted biomaterials. The pathogenicity of S. epidermidis is mostly due to its ability to form a thick, multilayered biofilm on polymeric surfaces [1–3]. Treatment of S. epidermidis infection has become a troublesome problem as biofilm-associated bacteria exhibit enhanced resistance to antibiotics and to components of the innate host defences [4, 5]. Among the Staphylococci, the other major human pathogen is Staphylococcus aureus, which causes infections ranging from cutaneous infections and food poisoning to life-threatening septicaemia. Aside from biofilm, S. aureus produce a large array of exotoxins and exoezymes .
Two-component regulatory systems (TCSs) play a pivotal role in bacterial adaptation, survival, and virulence by sensing changes in the external environment and modulating gene expression in response to a variety of stimuli [7–9]. Among the TCSs identified in the genomes of S. epidermidis, functions of LytSR are unknown, though in S. aureus LytSR has been demonstrated to play a role in bacterial autolysis and biofilm formation.
LytSR two-component regulatory system was firstly identified from the S. aureus genome. The lytS integration mutant of S. aureus strain NCTC 8325-4 exhibited a marked propensity to form aggregates in liquid culture and an increased rate of penicillin-and Triton X-100-induced lysis. In combination with subsequent zymographic analysis, it was suggested that LytSR is involved in either regulation of murein hydrolases gene expression or modulation of murein hydrolase activity . Recently, Shrama et al. reported that a lytS knockout mutant of S. aureus strain UAMS-1 produced more adherent biofilm .
In search of genes regulated by LytSR in S. aureus, two additional open reading frames immediately downstream from lytS and lytR were identified and designated gene lrgA and lrgB, whose transcription was positively regulated by LytSR and the global regulators Agr and SarA. It was proposed that LrgA, and possibly LrgB, functions in a similar way to an antiholin, i.e., blocking murein hydrolases access to the substrate peptidoglycan . Bayles et al. put forward the possibility that LrgAB exploits a molecular strategy, which is functionally analogous to that mediated by the eukaryotic Bcl-2 family of apoptosis regulatory proteins, to control bacterial programmed cell death [13, 14]. Recent study suggested that LytSR regulatory system sense a collapse in membrane potential and then induce the transcription of the lrgAB operon .
Several TCSs of S. aureus, such as agr and arlRS, have been proven to affect biofilm formation, whereas little has been known in the case of S. epidermidis. In S. aureus and S. epidermidis, an agr mutant forms a significantly thicker biofilm. However, the agr regulons of the two species comprise different genes. Autolysin E (AtlE) which has been documented to mediate initial attachment of S. epidermidis to a polymer surface, overexpresses in an agr mutant, whereas the homologus Atl protein in S. aureus is not under agr control [16, 17]. Previous studies have shown that arlS mutation in S. aureus enhanced biofilm formation on a polystyrene surface in a complex TSB medium . However, an arlS knockout mutant of S. epidermidis generated by our laboratory displayed significantly reduced ability of biofilm formation , which suggest S. aureus and S. epidermidis adopt different strategies to regulate biofilm formation even though the genome of S. epidermidis is highly homologous to that of S. aureus.
Therefore, to investigate the role of LytSR in bacterial autolysis and biofilm development in S. epidermidis, 1457ΔlytSRstrain was constructed. The transcriptional profile of 1457ΔlytSR was subsequently analyzed by DNA microarray and related functions were examined.
Construction of S. epidermidis 1457ΔlytSR and the complementation strain
Modulation of lytSR on murein hydrolase activity
Impact of lytSR knockout on S. epidermidis biofilm formation
Transcriptional profiling of 1457ΔlytSR strain
Genes expressed differentially in strain 1457ΔlytSR compared to the wild-type strain
Description or predicted function
Expression ratio (Mutant/WT)
Amino acid biosynthesis
5-methyltetrahydropteroyltriglutamate homocysteine methyltransferase
glutamate synthase large subunit
3-isopropylmalate dehydratase small subunit
imidazoleglycerol phosphate synthase, cyclase subunit
Amino acid transport
di-tripeptide transporter, putative
oligopeptide transport system permease protein OppC
peptide ABC transporter, ATP-binding protein, putative
Inorganic ion transport and metabolism
Na+/H+ antiporter, MnhD component, putative
Na+/H+ antiporter, MnhG component, putative
cobalt transport family protein
iron compound ABC transporter, iron
molybdenum transport ATP-binding protein
arsenical pump-driving ATPase
Phe-tRNA synthetase alpha chain
translation initiation factor IF-1
ribosomal protein L15
ribosomal protein L30
30 S ribosomal protein S5
50 S ribosomal protein L18
50 S ribosomal protein L6
30 S ribosomal protein S8
30 S ribosomal protein S14
50 S ribosomal protein L5
50 S ribosomal protein L14
50 S ribosomal protein L24
30 S ribosomal protein S17
50 S ribosomal protein L29
50 S ribosomal protein L16
30 S ribosomal protein S3
50 S ribosomal protein L22
30 S ribosomal protein S19
50 S ribosomal protein L2
50 S ribosomal protein L23
bifunctional GMP synthase/glutamine amidotransferase protein
carbamate kinase, putative
transcription regulator Crp/Fnr family protein
cytochrome d ubiquinol oxidase subunit II-like protein
nitrate reductase delta chain
nitrate reductase beta chain
nitrate reductase alpha chain
nitrite reductase nitrite reductase
putative carbamate kinase
DNA replication, recombination and repair
site-specific recombinase, resolvase family
SOS regulatory LexA protein
DNA replication protein DnaD, putative
type I restriction-modification system, R subunit
transcriptional regulator, MarR family
transcriptional regulator, AraC family
Expression of genes regulated by LytSR confirmed by RT Real-time PCR
n-fold(Real time PCR)
holin-like protein LrgA
0.133 (0.124, 0.143) ***
0.013 (0.008, 0.02) ***
0.476 (0.377, 0.601) **
cell wall enzyme EbsB, putative
0.278 (0.21, 0.369) **
3-isopropylmalate dehydratase small subunit
3.85 (3.595, 4.124) **
Pyruvate utilization of 1457 and 1457ΔlytSR
The capacity of Staphylococci to produce a biofilm is determined by environmental factors, such as glucose, osmolarity, ethanol, temperature and anaerobiosis etc, which suggests that there is a mechanism that senses and responds to extracellular signals . Two-component regulatory systems, composed of histidine kinases and their cognate response regulators, are the predominant means by which bacteria adapt to changes in their environment . Previous studies have shown yycG/yycF two-component system is essential for cell viability in B. subtilis and S. aureus and positively controls biofilm formation [22–24]. Another two TCSs of S. aureus, agr and arlRS, have also been proven to regulate biofilm formation [16–18].
Seventeen pairs of TCSs have been determined in the genome of S. epidermidis ATCC35984 (RP62A), while 16 pairs in ATCC12228 . We identified one pair of TCS encoding LytS and LytR homologs described in S. aureus. The LytSR two-component system in S. aureus has been viewed as an important regulator of bacterial autolysis . In the present study, the function of the S. epidermidis lytSR opreon was firstly investigated. The lytSR knockout mutation did not alter the susceptibility of strain 1457 to Triton X-100-induced lysis, which is different from the finding for S. aureus strain NCTC 8325-4 reported by Brunskill et al.. Recently, they found that in the strain UAMS-1, lytS knock-out did not result in spontaneous and Triton X-100-induced lysis increasing . The variation in susceptibility to Triton X-100-induced lysis between different staphylococcus strains could be explained partly by the fact that they represent different genetic background.
Since that lytS mutation in S. aureus has pleiotropic effects on different murein hydrolase activity , we hypothesized that in S. epidermidis, lytSR regulates murein hydrolase activity in a similar manner. Zymographic analysis revealed no significant differences between 1457ΔlytSR and the parent strain in the activities or expression of murein hydrolase isolated from both extracellular and cell wall fraction. However, quantification of the extracellular murein hydrolase activity produced by these strains demonstrated that 1457ΔlytSR produced diminished overall activity compared to that of the parental strain. As expected, microarray analysis revealed that lrgAB opreon was downregulated in 1457ΔlytSR. In S. aureus, LrgAB has a negative regulatory effect on extracellular murein hydrolase activity and disruption of lrgAB led to a significant increase in the activity [10, 12]. cidAB operon, which encodes the holin-like counterpart of the lrgAB operon, and alsSD operon, which encodes proteins involved in acetoin production, were then identified. Mutation of either cidAB or alsSD operon in the S. aureus strain UAMS-1 caused a dramatic decrease in extracellular murein hydrolase activity [26, 27]. We, therefore, speculate that in S. epidermidis some other LytSR regulated proteins similar to CidAB and/or AlsSD, may exist and overcome negative effect imposed by LrgAB on extracellular murein hydrolase activity, which warrants further investigation.
The role of cell death and lysis in bacterial adaptive responses to circumstances has been well elucidated in a number of bacteria, such as S. aureus and P. aeruginosa. Webb et al. proposed that in P. aeruginosa cell death benefited a subpopulation of surviving cells and therefore facilitated subsequent biofilm differentiation and dispersal [28–30]. Moreover, genomic DNA released following bacterial lysis constitutes the skeleton of biofilm. Since LytSR positively regulates the activity of extracellular murein hydrolases, it may affect cell viability and function in biofilm formation. By using the CLSM, significant decrease in red fluorescence was observed inside biofilm of 1457ΔlytSR, which indicated reduced loss of cell viability. Quantitative analysis showed that the percentage of dead cells inside biofilm of the wild type strain was approximately two times higher than that in the mutant. The results are consistent with the observation that 1457ΔlytSR displayed a reduction in activity of extracellular murein hydrolases. Disruption of either cidA or alsSD genes on the S. aureus chromosome resulted in significantly decreased extracellular murein hydrolase activity compared with that of the parental strain, UAMS-1. Both the cidA and the alsSD mutant displayed reduced cell death in stationary phase and completely abrogated cell lysis relative to UAMS-1 [26, 27]. Along these lines, the present study confirmed a connection between extracellular murein hydrolase activity and bacterial cell death. Furthermore, expression of cidC gene encoding pyruvate oxidase was found to be downregulated (5.07 fold) in 1457ΔlytSR through the microarray analysis. Deletion of cidC in S. aureus or S. pneumoniae caused reduced cell death and lysis in stationary phase[31, 32]. Based on these data, it was suggested LytSR may play an important role in bacterial cell death and lysis inside biofilm.
In this study, 1457ΔlytSRwas found to have growth defect in pyruvate fermentation broth and introducing plasmid encoding LytSR (pNS-lytSR) into the mutant completely restored the phenotype. Based on the fact that the wild-type strain and the mutant grow equally well in TSB containing 0.25% glucose. As we know, glucose is catabolized by glycolysis to pyruvate. If 1457ΔlytSRis impaired in its ability to metabolize pyruvate, then this would be reflected in the growth curve in TSB medium. The data actually indicated that 1457ΔlytSRis impaired in the transport of pyruvate and probably amino acids. Previous studies regarding bacterial cells taking up carboxylic acid from the surrounding medium have shown that pyruvate is actively transported across the bacterial membrane and that proton motive force (PMF) plays an important role in the process . In addition, transcription of genes involved in pyruvate metabolism such as mqo-3, mqo-2 and its neighbouring unknown gene SERP2169 were significantly downregulated in 1457ΔlytSR. These data along with the findings that in S. aureus LytSR responds to a collapse in Δψ by inducing the transcription of the lrgAB operon led us to hypothesize that LytSR accelerates pyruvate transport by sensing a reduction in PMF.
Compared to the parent stain, 1457ΔlytSRexhibited decreased expression of ribosomal genes and increased expression of amino acid biosynthetic genes, amino acyl-tRNA synthase genes, and amino acid transporters genes, which implies that lytSR mutation may induce a stringent response. Additionally, transcriptional profiling studies performed in Switzerland revealed that expression level of genes involved in stress response and cold shock was altered in the mutant. When bacteria encounter sudden unfavorable environment, protein synthesis will be inhibited, causing the induction or repression of many metabolic pathways according to physiological needs, and the induction of stationary-phase survival genes. This is called "the stringent response". Bacterial alarmone (p)ppGpp functions as a global regulator responsible for the stringent control. Two homologous (p)ppGpp synthetases, RelA and SpoT, have been identified and characterized in Escherichia coli[34–37]. Lemos et al. have reported that the relA mutation impaired the capacity of Streptococcus mutans to form biofilm. No changes in transcription of the relA/spoT homolog(s) were found in 1457ΔlytSR. However, SERP1879 encoding an AraC family transcriptional regulator was found to be upregulated significantly in the mutant. Transcriptional regulators of the AraC family are widespread among bacteria and have three main regulatory functions in common: carbon metabolism, stress response, and pathogenesis[39, 40].
Among the microarray data, several genes predicted to be involved in anaerobic metabolism were of particular interest. The arc operon encodes the enzymes of the arginine deiminase (ADI) pathway, which catalyzes the conversion of arginine into ornithine, ammonia, and CO2, with the concomitant production of 1 mol of ATP per mol of arginine consumed. In the absence of oxygen, the ADI pathway enables S. aureus to grow in the medium containing arginine . Recent studies demonstrated that the arc operon identified in the genome of S epidermidis strain ATCC12228 but not in RP62A is located on a novel genomic island termed arginine catabolic mobile element (ACME). Except for the ACME-encoded arc operon, all S. epidermidis carry a native arc operon on the core chromosome. Diep et al. supposed that ACME-encoded gene products might confer survival advantage of S. aureus strain USA300 and other ACME-bearing staphylococci within the host, resulting in the widespread dissemination of bacterial progeny [42–44]. In the present study, arginine deiminase activity was performed as previously described [45, 46] and 1457ΔlytSR exhibited a reduced enzyme activity (Additional file 2, Figure S2).
In the present study, 1457ΔlytSR produced slightly more biofilm than its parent strain. However, no genes that are involved in biofilm formation directly, such as ica operon encoding enzymes responsible for PIA synthesis, were identified in the transcriptional profile. It was observed that ica transcription level and PIA production were similar between 1457ΔlytSR and its parent strain. Both tricarboxylic acid cycle stress and anaerobic condition have been proven to induce PIA production and promotion of biofilm, suggesting that changes in the metabolic status can be sensed and regulate biofilm formation [47, 48]. Moreover, the stringent response has also been demonstrated to affect biofilm formation. It suggests that lytSR mutation may indirectly enhance biofilm formation by altering the metabolic status of S. epidermidis.
The present study suggests that in S. epidermidis the LytSR two-component regulatory system play an important role in controlling extracellular murein hydrolase activity and bacterial cell death but has limited effect on autolysis. The lytSR mutation invokes a stringent type transcriptional profile, moreover, enhances biofilm formation, which suggests LytSR may function to indirectly regulate biofilm formation by altering the metabolic status of the bacteria, particularly under conditions in which supply of nutrient and oxygen is limited, such as the conditions in biofilm.
Bacterial strains, plasmids and growth media
Bacterial Strains and plasmids used in this study
Strain or plasmid
Source or reference
S. aureus RN4220
Restriction-negative, intermediate host for plasmid transfer from E. coli to S. epidermidis
Biofilm-positive laboratory strain
lytSR: : erm derivative of S. epidermidis 1457
lytSR complementary strain
1457 ΔlytSR ( pNS)
lytSR mutant containing the empty cloning vector
atlE: : erm derivative of S. epidermidis 1457
Biofilm-negative standard strain
Temperature-sensitive E. coli-Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus)
pBluescript KS+ derivative. Source of ermB gene (Emr). Apr
Deletion vector for lytSR; ermB fragment flanked by fragments upstream and downstream of lytSR in pBT2
E. coli-Staphylococcus shuttle cloning vector. Apr (E. coli) Spcr (Staphylococcus)
Plasmid pNS containing lytSR fragment and its native promoter
Construction of the S. epidermidis lytSR knockout mutant
Primers used in this study
Primers used for PCR products in allelic gene replacement
lyt- UF (upstream fragment)
lyt- UR (upstream fragment)
lyt- DF (downstream fragment)
lyt- DR (downstream fragment))
Primers used in complementation of 1457 ΔytSR1 strain
Primers used for real time RT-PCR in confirmation of microarray data
Complementation of 1457ΔlytSR with pNS-lytSR
For complementation of 1457ΔlytSR strain, the staphylococcus cloning vector pCN51 was modified by replacing the erythromycin-resistance cassette with the spectinomycin-resistance cassette, named as pNS . The lytSR operon encompassing its promoter and ribosome binding site was amplified by PCR with primers lyt-CF and lyt-CR. The resulting PCR product was then ligated into BamHI and KpnI sites of the pNS vector. The recombinant plasmid allowed the expression of lytSR under the control of its native promoter, named as pNS-lytSR. The promoter sequences were predicted by using BDGP Neural Network Promoter Prediction software http://www.fruitfly.org/seq_tools/promoter.html. Meantime, the empty vector pNS was electroporated into 1457ΔlytSRas a control.
Morphology of 1457ΔlytSR observed with transmission electron microscopy
Strains of S. epidermidis 1457, ΔlytSR and ΔatlE were cultured in TSB medium for 16 hours, and resuspended in 2.5% glutaraldehyde in Dulbecco's phosphate-buffered saline (PBS) overnight. After postfixation in osmium tetroxide, the preparations were dehydrated with increasing alcohol concentrations and embedded in Epon 812. Thin sections were cut using a Leica Ultracut R at a thickness of 70 nm, stained with 1% uranyl acetate-lead acetate and examined with a Philips Tecnai-12 Biotwin transmission electron microscope.
Triton X-100 induced autolysis
To examine the potential role of lytSR in the regulation of autolysis in Staphylococcus epidermidis, Triton X-100-induced autolysis of 1457ΔlytSR was performed as described by Brunskill & Bayles . Bacterial cells of 50 ml were collected from early exponentially growing cultures (OD600 = 0.7) containing 1 M NaCl, and the cells were pelleted by centrifugation. The cells were washed twice with 50 ml of ice-cold water and resuspended in 50 ml of Tris-HCl (pH 7.2) containing 0.05% (vol/vol) Triton X-100. Autolysis was measured during incubation at 37 °C as the decrease in turbidity at 600 nm, using a model 6131 Biophotometer (Eppendorf, Hamburg, Germany).
To determine if the lytSR mutation affects murein hydrolase activity, zymographic analysis of extracellular, cell wall-associated murein hydrolases from strains 1457 and 1457ΔlytSR grown in TSB medium was carried out essentially as described previously [12, 51]. Cell-wall-associated murein hydrolases were extracted with 4% SDS. Briefly bacteria cells from overnight cultures were pelleted down, washed twice with 100 mM phosphate buffer and resuspended by 100 mM sodium phosphate buffer containing 4% SDS in amount about equal to wet weight of pellet. The cell suspension was incubated at 37 °C water bath for 10 min. The supernatant containing surface proteins were collected after centrifugation. Extracellular and cell surface proteins extracted were separated in SDS-polyacrylamide gel electrophoresis gels containing 2.0 mg of M. luteus or S. epidermidis cells/ml. Murein hydrolase activity was detected by incubation overnight at 37 °C in a buffer containing Triton X-100, followed by staining with methylene blue.
Cell wall hydrolysis assays
To quantify the amount of hydrolysis observed in the zymographic analysis, cell wall hydrolysis assays were examined as described by Groicher et al. . Extracellular murein hydrolases of bacteria were isolated from 15 ml of a 16-h culture by centrifugation at 6,000 g for 15 min at 4 °C. The supernatant was filter-sterilized and concentrated 100-fold using a Amicon Ultra-15 Centrifugal Filter unit (Milipore, 5 kD). The concentration of total proteins in each preparation was determined using the Bradford assay according to the manufacturer's directions. Briefly, 100 μg of enzyme extract was added to a suspension of autoclaved and lyophilized M. luteus or S. epidermidis cells (1.0 mg/ml) in 100 mM Tris-HCl (pH 8.0) and incubated at 37 °C with shaking. Cell wall hydrolysis was measured as decrease in turbidity at 600 nm every 30 min, using a model 6131 Biophotometer (Ependorf, Hamburg, Germany).
Detection of Biofilm formation
To investigate the ability of 1457ΔlytSR to form biofilm, the standard microtiter-plate test was carried out essentially as described by Christensen et al. . Briefly, overnight cultures of S. epidermidis strains grown in TSB medium were diluted 1:200 and inoculated into wells of polystyrene microtiter plates (200 μl per well) and incubated at 37 °C for 24 h. After incubation, the wells were washed gently three times with 200 μl sterile PBS, air-dried and stained with 2% crystal violet for 5 min. Then, the plate was rinsed under running tap water, the crystal violet was redissolved in ethanol and the absorbance was determined at 570 nm.
To determine whether lytSR affects cell viability in biofilm, bacterial cells were cultivated in cover-glass cell-culture dish (WPI, Sarasota, FL, USA) as described previously . Briefly, overnight cultures of S. epidermidis strains grown in TSB medium were diluted 1:200, then inoculated into the dish (2 ml per dish) and incubated at 37 °C. After 24 hours, the dish was washed gently three times with 1 ml sterile 0.85% NaCl, then stained by SYTO 9 and PI for 15 min and examined by Leica TCS SP5 confocal microscope.
Quantitative analysis of bacterial cell death inside biofilms
To quantify relative viability of S. epidermidis strains, live/dead stained biofilms were scraped from the dish and dispersed thoroughly by pipetting. The integrated intensities (1 second) of the green (SYTO 9, 535 nm) and red (PI, 625 nm) emission of suspensions excited at 485 nm were measured respectively by Beckman Coulter DTX880 multimode detectors. The red/green fluorescence ratios (RatioR/G) were calculated, and a standard curve of Ratio R/G versus percentage of dead cells in the S. epidermidis suspension was plotted as described in the manuals of LIVE/DEAD® BacLight™Bacterial Viability Kit L7012 (Invitrogen, Carlsbad, USA). The percentage of dead cells inside biofilms was determined by comparison to the standard curve.
Pyruvate utilization test
To verify physiological changes of 1457ΔlytSR detected by GPI-vitek test system, overnight cultures of S. epidermidis were diluted 1:200 into Pyruvate fermentation broth (Tryptone 10 g, Pyruvate 10 g, Yeast extract 5 g, Dipotassium phosphate 5 g, Sodium chloride 5 g per liter, pH 7.4) and incubated microaerobically at 37 °C . The growth was detected by monitoring turbidity of the cultures at 600 nm.
RNA extraction and Microarray analysis
Overnight cultures of S. epidermidis 1457 and 1457ΔlytSR were diluted 1:200 into fresh TSB and grown at 37 °C to an OD600 of 3.0 (mid-exponential growth). Eight millilitres of bacterial cultures were pelleted, washed with ice-cold saline, and then homogenized using 0.1 mm Ziconia-silica beads in Mini-Beadbeater (Biospec) at a speed of 4800 rpm. The bacterial RNA was isolated using a QIAGEN RNeasy kit according to the standard QIAGEN RNeasy protocol.
The custom-made S. epidermidis GeneChips (Shanghai Biochip Co., Ltd) included qualifiers representing open reading frame (ORF) sequences identified in the genomes of the S. epidermidis strain RP62A, as well as unique ORFs in S. epidermidis strain 12228. The GeneChips were composed of cDNA array containing PCR products of 2316 genes and oligonucleotide array containing 252 genes. Reverse transcription were performed using 2 μg of total RNA using T7 promoter primers and M-MLV reverse transcriptase (Promega, Madison, WI, USA), and then cRNA was transcribed from the resulting cDNA as template. cRNA prepared form 1457ΔlytSR and the parent strain was labelled using the dyes Cy3 and Cy5 according to the manufacturer's instructions(Amersham, Piscataway, New Jersey) respectively. Microarray hybridization (at 42 °C for 16 h) and washing of the slides at 50 °C were performed according to the manufacturer's instructions. Hybridized slides were scanned by Agilent Scanner (G2655AA) at a 10-μm resolution. Data of each image were normalized to the mean ratio of means of all features. Mean values and standard deviations of gene expression ratios based on three spot replicates on each microarray were calculated in Microsoft Excel XP. The complete set of microarray data was deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO, available at http://www.ncbi.nlm.nih.gov/geo/ and is accessible through GEO Series accession number GSE20652.
Validation of microarray data by Real time PCR
To confirm the results of the microarray data, the relative expression levels of the lrgA, ebsB, arcA, serp2169 and leuC genes were determined by real-time PCR with gene-specific primers, designed according to the genomic sequence of S. epidermidis RP62A (GenBank accession number CP000029). The sequences of the primers are shown in Table 4. Briefly, DNase-treated RNA was reverse transcribed using M-MLV and a hexamer random primer mix. Appropriate concentration of cDNA sample was then used for real-time PCR using an ABI 7500 real-time PCR detection system, gene-specific primers, and the SYBR Green I mixture (Takara, Dalian, China). Relative expression levels were determined by comparison to the level of gyrB expression in the same cDNA preparations.
Experimental data obtained were analyzed with the SPSS software and compared by Student's t test. Differences with P < 0.05 were considered statistically significant.
We thank Dr. Patrice Francois (Genomic Research Laboratory, University of Geneva Hospitals, Switzerland) for repeating the microarray experiments.
This work was supported by the 11th Five-Year Plan of the Ministry of Sciences and Technology (2010DFA32100, 2009ZX09303-005, 2008ZX10003-016), the Hi-Tech Program of China (863) (2006AA02A253), the Scientific Technology Development Foundation of Shanghai (08JC1401600, 10410700600), National Natural Science Foundation of China (30800036), the Research Initiation Grant for Young Faculty of Fudan University (09FQ43).
- Ziebuhr W, Heilmann C, Gotz F, Meyer P, Wilms K, Straube E, Hacker J: Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infection and immunity. 1997, 65 (3): 890-896.PubMed CentralPubMedGoogle Scholar
- Rupp ME, Archer GL: Coagulase-negative staphylococci: pathogens associated with medical progress. Clin Infect Dis. 1994, 19 (2): 231-243; quiz 244-235.View ArticlePubMedGoogle Scholar
- Bowden MG, Chen W, Singvall J, Xu Y, Peacock SJ, Valtulina V, Speziale P, Hook M: Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidis. Microbiology (Reading, England). 2005, 151 (Pt 5): 1453-1464.View ArticleGoogle Scholar
- Vuong C, Kocianova S, Voyich JM, Yao Y, Fischer ER, DeLeo FR, Otto M: A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence. The Journal of biological chemistry. 2004, 279 (52): 54881-54886. 10.1074/jbc.M411374200.View ArticlePubMedGoogle Scholar
- Donlan RM, Costerton JW: Biofilms: survival mechanisms of clinically relevant microorganisms. Clinical microbiology reviews. 2002, 15 (2): 167-193. 10.1128/CMR.15.2.167-193.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang YQ, Ren SX, Li HL, Wang YX, Fu G, Yang J, Qin ZQ, Miao YG, Wang WY, Chen RS: Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Molecular microbiology. 2003, 49 (6): 1577-1593. 10.1046/j.1365-2958.2003.03671.x.View ArticlePubMedGoogle Scholar
- Stock AM, Robinson VL, Goudreau PN: Two-component signal transduction. Annual review of biochemistry. 2000, 69: 183-215. 10.1146/annurev.biochem.69.1.183.View ArticlePubMedGoogle Scholar
- Skerker JM, Prasol MS, Perchuk BS, Biondi EG, Laub MT: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis. PLoS biology. 2005, 3 (10): e334.-10.1371/journal.pbio.0030334.PubMed CentralView ArticlePubMedGoogle Scholar
- Bader MW, Sanowar S, Daley ME, Schneider AR, Cho U, Xu W, Klevit RE, Le Moual H, Miller SI: Recognition of antimicrobial peptides by a bacterial sensor kinase. Cell. 2005, 122 (3): 461-472. 10.1016/j.cell.2005.05.030.View ArticlePubMedGoogle Scholar
- Brunskill EW, Bayles KW: Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus. Journal of bacteriology. 1996, 178 (3): 611-618.PubMed CentralPubMedGoogle Scholar
- Sharma Kuinkel BK, Mann EE, Ahn JS, Kuechenmeister LJ, Dunman PM, Bayles KW: The Staphylococcus aureus LytSR two-component regulatory system affects biofilm formation. Journal of bacteriology. 2009, 191 (15): 4767-4775. 10.1128/JB.00348-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Groicher KH, Firek BA, Fujimoto DF, Bayles KW: The Staphylococcus aureus lrgAB operon modulates murein hydrolase activity and penicillin tolerance. Journal of bacteriology. 2000, 182 (7): 1794-1801. 10.1128/JB.182.7.1794-1801.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Bayles KW: Are the molecular strategies that control apoptosis conserved in bacteria?. Trends in microbiology. 2003, 11 (7): 306-311. 10.1016/S0966-842X(03)00144-6.View ArticlePubMedGoogle Scholar
- Rice KC, Bayles KW: Death's toolbox: examining the molecular components of bacterial programmed cell death. Molecular microbiology. 2003, 50 (3): 729-738. 10.1046/j.1365-2958.2003.t01-1-03720.x.View ArticlePubMedGoogle Scholar
- Patton TG, Yang SJ, Bayles KW: The role of proton motive force in expression of the Staphylococcus aureus cid and lrg operons. Molecular microbiology. 2006, 59 (5): 1395-1404. 10.1111/j.1365-2958.2006.05034.x.View ArticlePubMedGoogle Scholar
- Kong KF, Vuong C, Otto M: Staphylococcus quorum sensing in biofilm formation and infection. Int J Med Microbiol. 2006, 296 (2-3): 133-139. 10.1016/j.ijmm.2006.01.042.View ArticlePubMedGoogle Scholar
- Boles BR, Horswill AR: Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS pathogens. 2008, 4 (4): e1000052.-10.1371/journal.ppat.1000052.PubMed CentralView ArticlePubMedGoogle Scholar
- Toledo-Arana A, Merino N, Vergara-Irigaray M, Debarbouille M, Penades JR, Lasa I: Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system. Journal of bacteriology. 2005, 187 (15): 5318-5329. 10.1128/JB.187.15.5318-5329.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang J, Zhu T, Lou Q, Wu Y, Han C, Qu D: Biological functions of arlS gene of two-component signal transduction system in Staphylococcus epidermids. Chinese Journal of Microbiology and Immunology. 2007, 27 (10): .-Google Scholar
- Brunskill EW, Bayles KW: Identification of LytSR-regulated genes from Staphylococcus aureus. Journal of bacteriology. 1996, 178 (19): 5810-5812.PubMed CentralPubMedGoogle Scholar
- Lim Y, Jana M, Luong TT, Lee CY: Control of glucose-and NaCl-induced biofilm formation by rbf in Staphylococcus aureus. Journal of bacteriology. 2004, 186 (3): 722-729. 10.1128/JB.186.3.722-729.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Howell A, Dubrac S, Andersen KK, Noone D, Fert J, Msadek T, Devine K: Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach. Molecular microbiology. 2003, 49 (6): 1639-1655. 10.1046/j.1365-2958.2003.03661.x.View ArticlePubMedGoogle Scholar
- Fabret C, Hoch JA: A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. Journal of bacteriology. 1998, 180 (23): 6375-6383.PubMed CentralPubMedGoogle Scholar
- Dubrac S, Boneca IG, Poupel O, Msadek T: New insights into the WalK/WalR (YycG/YycF) essential signal transduction pathway reveal a major role in controlling cell wall metabolism and biofilm formation in Staphylococcus aureus. Journal of bacteriology. 2007, 189 (22): 8257-8269. 10.1128/JB.00645-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Qin Z, Zhong Y, Zhang J, He Y, Wu Y, Jiang J, Chen J, Luo X, Qu D: Bioinformatics analysis of two-component regulatory systems in Staphylococcus epidermidis. CHINESE SCIENCE BULLETIN. 2004, 49 (12): 1267-1271. 10.1360/03wc0384.View ArticleGoogle Scholar
- Rice KC, Firek BA, Nelson JB, Yang SJ, Patton TG, Bayles KW: The Staphylococcus aureus cidAB operon: evaluation of its role in regulation of murein hydrolase activity and penicillin tolerance. Journal of bacteriology. 2003, 185 (8): 2635-2643. 10.1128/JB.185.8.2635-2643.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Yang SJ, Dunman PM, Projan SJ, Bayles KW: Characterization of the Staphylococcus aureus CidR regulon: elucidation of a novel role for acetoin metabolism in cell death and lysis. Molecular microbiology. 2006, 60 (2): 458-468. 10.1111/j.1365-2958.2006.05105.x.View ArticlePubMedGoogle Scholar
- Webb JS, Thompson LS, James S, Charlton T, Tolker-Nielsen T, Koch B, Givskov M, Kjelleberg S: Cell death in Pseudomonas aeruginosa biofilm development. Journal of bacteriology. 2003, 185 (15): 4585-4592. 10.1128/JB.185.15.4585-4592.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Qin Z, Ou Y, Yang L, Zhu Y, Tolker-Nielsen T, Molin S, Qu D: Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis. Microbiology (Reading, England). 2007, 153 (Pt 7): 2083-2092.View ArticleGoogle Scholar
- Rice KC, Mann EE, Endres JL, Weiss EC, Cassat JE, Smeltzer MS, Bayles KW: The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus. Proceedings of the National Academy of Sciences of the United States of America. 2007, 104 (19): 8113-8118. 10.1073/pnas.0610226104.PubMed CentralView ArticlePubMedGoogle Scholar
- Regev-Yochay G, Trzcinski K, Thompson CM, Lipsitch M, Malley R: SpxB is a suicide gene of Streptococcus pneumoniae and confers a selective advantage in an in vivo competitive colonization model. Journal of bacteriology. 2007, 189 (18): 6532-6539. 10.1128/JB.00813-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Patton TG, Rice KC, Foster MK, Bayles KW: The Staphylococcus aureus cidC gene encodes a pyruvate oxidase that affects acetate metabolism and cell death in stationary phase. Molecular microbiology. 2005, 56 (6): 1664-1674. 10.1111/j.1365-2958.2005.04653.x.View ArticlePubMedGoogle Scholar
- Tsau J-L, Guffanti AA, Montville TJ: Pyruvate is transported by a proton symport inLactobacillus plantarum 8014. Current Microbiology. 1992, 25 (1): 47-50. 10.1007/BF01570082.View ArticleGoogle Scholar
- Potrykus K, Cashel M: (p)ppGpp: still magical?. Annu Rev Microbiol. 2008, 62: 35-51. 10.1146/annurev.micro.62.081307.162903.View ArticlePubMedGoogle Scholar
- Metzger S, Dror IB, Aizenman E, Schreiber G, Toone M, Friesen JD, Cashel M, Glaser G: The nucleotide sequence and characterization of the relA gene of Escherichia coli. J Biol Chem. 1988, 263 (30): 15699-15704.PubMedGoogle Scholar
- Sarubbi E, Rudd KE, Xiao H, Ikehara K, Kalman M, Cashel M: Characterization of the spoT gene of Escherichia coli. J Biol Chem. 1989, 264 (25): 15074-15082.PubMedGoogle Scholar
- ASM Press, Cashel M, Gentry DR, Hernandez VJ, D V: The stringent response. Escherichia coli and Salmonella: Cellular and molecular biology. Edited by: Neidhardt FC. 1996, ASM Press, 1: 1458-1496.Google Scholar
- Lemos JA, Brown TA, Burne RA: Effects of RelA on key virulence properties of planktonic and biofilm populations of Streptococcus mutans. Infection and immunity. 2004, 72 (3): 1431-1440. 10.1128/IAI.72.3.1431-1440.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Frota CC, Papavinasasundaram KG, Davis EO, Colston MJ: The AraC family transcriptional regulator Rv1931c plays a role in the virulence of Mycobacterium tuberculosis. Infection and immunity. 2004, 72 (9): 5483-5486. 10.1128/IAI.72.9.5483-5486.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Gallegos MT, Schleif R, Bairoch A, Hofmann K, Ramos JL: Arac/XylS family of transcriptional regulators. Microbiol Mol Biol Rev. 1997, 61 (4): 393-410.PubMed CentralPubMedGoogle Scholar
- Makhlin J, Kofman T, Borovok I, Kohler C, Engelmann S, Cohen G, Aharonowitz Y: Staphylococcus aureus ArcR controls expression of the arginine deiminase operon. Journal of bacteriology. 2007, 189 (16): 5976-5986. 10.1128/JB.00592-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Diep BA, Stone GG, Basuino L, Graber CJ, Miller A, des Etages SA, Jones A, Palazzolo-Ballance AM, Perdreau-Remington F, Sensabaugh GF: The arginine catabolic mobile element and staphylococcal chromosomal cassette mec linkage: convergence of virulence and resistance in the USA300 clone of methicillin-resistant Staphylococcus aureus. The Journal of infectious diseases. 2008, 197 (11): 1523-1530. 10.1086/587907.View ArticlePubMedGoogle Scholar
- Diep BA, Gill SR, Chang RF, Phan TH, Chen JH, Davidson MG, Lin F, Lin J, Carleton HA, Mongodin EF: Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus. Lancet. 2006, 367 (9512): 731-739. 10.1016/S0140-6736(06)68231-7.View ArticlePubMedGoogle Scholar
- Miragaia M, de Lencastre H, Perdreau-Remington F, Chambers HF, Higashi J, Sullam PM, Lin J, Wong KI, King KA, Otto M: Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis. PloS one. 2009, 4 (11): e7722.-10.1371/journal.pone.0007722.PubMed CentralView ArticlePubMedGoogle Scholar
- Sugawara K, Yoshizawa Y, Tzeng S, Epstein WL, Fukuyama K: Colorimetric determination of citrulline residues in proteins. Analytical biochemistry. 1998, 265 (1): 92-96. 10.1006/abio.1998.2925.View ArticlePubMedGoogle Scholar
- Zhu Y, Weiss EC, Otto M, Fey PD, Smeltzer MS, Somerville GA: Staphylococcus aureus biofilm metabolism and the influence of arginine on polysaccharide intercellular adhesin synthesis, biofilm formation, and pathogenesis. Infection and immunity. 2007, 75 (9): 4219-4226. 10.1128/IAI.00509-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Vuong C, Kidder JB, Jacobson ER, Otto M, Proctor RA, Somerville GA: Staphylococcus epidermidis polysaccharide intercellular adhesin production significantly increases during tricarboxylic acid cycle stress. Journal of bacteriology. 2005, 187 (9): 2967-2973. 10.1128/JB.187.9.2967-2973.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Cramton SE, Ulrich M, Gotz F, Doring G: Anaerobic conditions induce expression of polysaccharide intercellular adhesin in Staphylococcus aureus and Staphylococcus epidermidis. Infection and immunity. 2001, 69 (6): 4079-4085. 10.1128/IAI.69.6.4079-4085.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Bruckner R: Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus. FEMS microbiology letters. 1997, 151 (1): 1-8. 10.1016/S0378-1097(97)00116-X.View ArticlePubMedGoogle Scholar
- Charpentier E, Anton AI, Barry P, Alfonso B, Fang Y, Novick RP: Novel cassette-based shuttle vector system for gram-positive bacteria. Applied and environmental microbiology. 2004, 70 (10): 6076-6085. 10.1128/AEM.70.10.6076-6085.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Heilmann C, Hussain M, Peters G, Gotz F: Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface. Molecular microbiology. 1997, 24 (5): 1013-1024. 10.1046/j.1365-2958.1997.4101774.x.View ArticlePubMedGoogle Scholar
- Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH: Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of clinical microbiology. 1985, 22 (6): 996-1006.PubMed CentralPubMedGoogle Scholar
- Gross KC, Houghton MP, Senterfit LB: Presumptive speciation of Streptococcus bovis and other group D streptococci from human sources by using arginine and pyruvate tests. Journal of clinical microbiology. 1975, 1 (1): 54-60.PubMed CentralPubMedGoogle Scholar
- Kreiswirth BN, Lofdahl S, Betley MJ, O'Reilly M, Schlievert PM, Bergdoll MS, Novick RP: The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature. 1983, 305 (5936): 709-712. 10.1038/305709a0.View ArticlePubMedGoogle Scholar
- Mack D, Siemssen N, Laufs R: Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion. Infection and immunity. 1992, 60 (5): 2048-2057.PubMed CentralPubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.