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Table 4 Primers used in this study

From: Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile

Primers

Sequence(5'→3')*

Restriction

Primers used for PCR products in allelic gene replacement

lyt- UF (upstream fragment)

CCGGAATTC GAACCGATGGACCAGTAG

BamHI

lyt- UR (upstream fragment)

CGGAATTC TAAAGAGGGACGACAATGG

EcoRI

lyt- DF (downstream fragment)

CCCAAGCTT CAACAACTCGGTCTTCAA

HindIII

lyt- DR (downstream fragment))

CTAGCTAGC AAAGGTATGGGAATGACG

NheI

Primers used in complementation of 1457 ΔytSR1 strain

lyt- CF

GGGGTACC TTATTGAAGACCGAGTTGTTGTTTA

BamHI

lyt- CR

CGGGATCC TATGAAACAAGCCAATGTAAGTGC

KpnI

Primers used for real time RT-PCR in confirmation of microarray data

gyrB- RF

TTTCACTTTCTTCAGGGTTCTTAC

 

gyrB-RR

CCATCTGTAGGACGCATTATTG

 

lrgA- RF

GCATTGTGAAATTAGGTCAAGTTG

 

lrgA-RR

ACTAATAATTGTGACGCAAAGCC

 

serp2169- RF

GCATCCGCTTCTCCAATATCTG

 

serp2169-RR

TAAACAACATACACACGCTAAACC

 

ebsB- RF

TTTGATGCTGCGACTAAAGG

 

ebsB-RR

CATTGCTGCCCATTCTGC

 

arcA- RF

GGCTGACTCATACATCTTGG

 

arcA-RR

GGGTTGTGGTGACATACG

 

leuC- RF

CCAGGATGTTCTATGTGCTTAGG

 

leuC-RR

CGCCTTTGCCTTGTCTTCC

 
  1. * Primers were designed according to the genomic sequence of S. epidermidis RP62A (GenBank accession number CP000029).