Primers | Sequence(5'→3')* | Restriction |
---|---|---|
Primers used for PCR products in allelic gene replacement | ||
lyt- UF (upstream fragment) | CCGGAATTC GAACCGATGGACCAGTAG | BamHI |
lyt- UR (upstream fragment) | CGGAATTC TAAAGAGGGACGACAATGG | EcoRI |
lyt- DF (downstream fragment) | CCCAAGCTT CAACAACTCGGTCTTCAA | HindIII |
lyt- DR (downstream fragment)) | CTAGCTAGC AAAGGTATGGGAATGACG | NheI |
Primers used in complementation of 1457 ΔytSR1 strain | ||
lyt- CF | GGGGTACC TTATTGAAGACCGAGTTGTTGTTTA | BamHI |
lyt- CR | CGGGATCC TATGAAACAAGCCAATGTAAGTGC | KpnI |
Primers used for real time RT-PCR in confirmation of microarray data | ||
gyrB- RF | TTTCACTTTCTTCAGGGTTCTTAC | Â |
gyrB-RR | CCATCTGTAGGACGCATTATTG | Â |
lrgA- RF | GCATTGTGAAATTAGGTCAAGTTG | Â |
lrgA-RR | ACTAATAATTGTGACGCAAAGCC | Â |
serp2169- RF | GCATCCGCTTCTCCAATATCTG | Â |
serp2169-RR | TAAACAACATACACACGCTAAACC | Â |
ebsB- RF | TTTGATGCTGCGACTAAAGG | Â |
ebsB-RR | CATTGCTGCCCATTCTGC | Â |
arcA- RF | GGCTGACTCATACATCTTGG | Â |
arcA-RR | GGGTTGTGGTGACATACG | Â |
leuC- RF | CCAGGATGTTCTATGTGCTTAGG | Â |
leuC-RR | CGCCTTTGCCTTGTCTTCC | Â |