Skip to main content

Advertisement

Table 3 Bacterial Strains and plasmids used in this study

From: Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile

Strain or plasmid Relevant characteristic(s) Source or reference
Strains   
S. aureus RN4220 Restriction-negative, intermediate host for plasmid transfer from E. coli to S. epidermidis [54]
S. epidermidis   
   1457 Biofilm-positive laboratory strain [55]
   1457 ΔlytSR lytSR: : erm derivative of S. epidermidis 1457 This study
   1457ΔlytSR (pNS-lytSR) lytSR complementary strain This study
   1457 ΔlytSR ( pNS) lytSR mutant containing the empty cloning vector This study
   1457 ΔatlE atlE: : erm derivative of S. epidermidis 1457 [29]
   12228 Biofilm-negative standard strain [6]
Plasmids   
pBT2 Temperature-sensitive E. coli-Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus) [49]
pEC1 pBluescript KS+ derivative. Source of ermB gene (Emr). Apr [49]
pBT2-ΔlytSR Deletion vector for lytSR; ermB fragment flanked by fragments upstream and downstream of lytSR in pBT2 This study
pNS E. coli-Staphylococcus shuttle cloning vector. Apr (E. coli) Spcr (Staphylococcus) This study
pNS-lytSR Plasmid pNS containing lytSR fragment and its native promoter This study
  1. * Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Em, erythromycin; Spc, spectinomycin