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Table 3 Bacterial Strains and plasmids used in this study

From: Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile

Strain or plasmid

Relevant characteristic(s)

Source or reference

Strains

  

S. aureus RN4220

Restriction-negative, intermediate host for plasmid transfer from E. coli to S. epidermidis

[54]

S. epidermidis

  

   1457

Biofilm-positive laboratory strain

[55]

   1457 ΔlytSR

lytSR: : erm derivative of S. epidermidis 1457

This study

   1457ΔlytSR (pNS-lytSR)

lytSR complementary strain

This study

   1457 ΔlytSR ( pNS)

lytSR mutant containing the empty cloning vector

This study

   1457 ΔatlE

atlE: : erm derivative of S. epidermidis 1457

[29]

   12228

Biofilm-negative standard strain

[6]

Plasmids

  

pBT2

Temperature-sensitive E. coli-Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus)

[49]

pEC1

pBluescript KS+ derivative. Source of ermB gene (Emr). Apr

[49]

pBT2-ΔlytSR

Deletion vector for lytSR; ermB fragment flanked by fragments upstream and downstream of lytSR in pBT2

This study

pNS

E. coli-Staphylococcus shuttle cloning vector. Apr (E. coli) Spcr (Staphylococcus)

This study

pNS-lytSR

Plasmid pNS containing lytSR fragment and its native promoter

This study

  1. * Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Em, erythromycin; Spc, spectinomycin