Strain or plasmid | Relevant characteristic(s) | Source or reference |
---|---|---|
Strains | Â | Â |
S. aureus RN4220 | Restriction-negative, intermediate host for plasmid transfer from E. coli to S. epidermidis | [54] |
S. epidermidis | Â | Â |
   1457 | Biofilm-positive laboratory strain | [55] |
   1457 ΔlytSR | lytSR: : erm derivative of S. epidermidis 1457 | This study |
   1457ΔlytSR (pNS-lytSR) | lytSR complementary strain | This study |
   1457 ΔlytSR ( pNS) | lytSR mutant containing the empty cloning vector | This study |
   1457 ΔatlE | atlE: : erm derivative of S. epidermidis 1457 | [29] |
   12228 | Biofilm-negative standard strain | [6] |
Plasmids | Â | Â |
pBT2 | Temperature-sensitive E. coli-Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus) | [49] |
pEC1 | pBluescript KS+ derivative. Source of ermB gene (Emr). Apr | [49] |
pBT2-ΔlytSR | Deletion vector for lytSR; ermB fragment flanked by fragments upstream and downstream of lytSR in pBT2 | This study |
pNS | E. coli-Staphylococcus shuttle cloning vector. Apr (E. coli) Spcr (Staphylococcus) | This study |
pNS-lytSR | Plasmid pNS containing lytSR fragment and its native promoter | This study |