Figure 7From: Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profileEffect of lytSR gene knocking out on S. epidermidis biofilm formation. The biofilm formation of S. epidermidis ΔlytSR and its parent strain was detected by semi-quantitative microtiter plate assay. Briefly, the overnight bacterial were diluted by 1:200 and cultured in 96-well plate (200 μl/well) at 37 °C for 24 h. The well was washed by PBS for 3 times, fixed by 99% methanol and stained with crystal violet. Data are means ± SD of 3 independent experiments. *P < 0.05; ΔlytSR vs. WT; ΔlytSR(pNS-lytSR) vs. ΔlytSR(pNS-lytSR).Back to article page