Identification of two Mycobacterium tuberculosis H37Rv ORFs involved in resistance to killing by human macrophages
© Miller and Shinnick; licensee BioMed Central Ltd. 2001
Received: 6 September 2001
Accepted: 17 October 2001
Published: 17 October 2001
The ability of Mycobacterium tuberculosis to survive and replicate in macrophages is crucial for the mycobacterium's ability to infect the host and cause tuberculosis. To identify Mycobacterium tuberculosis genes involved in survival in macrophages, a library of non-pathogenic Mycobacterium smegmatis bacteria, each carrying an individual integrated cosmid containing M. tuberculosis H37Rv genomic DNA, was passed through THP-1 human macrophages three times.
Two of the clones recovered from this enrichment process, sur2 and sur3, exhibited significantly increased survival relative to wild-type bacteria. In coinfection experiments, the ratio of sur2 colonies to wild-type colonies was 1:1 at 0 hours but increased to 20:1 at 24 hours post phagocytosis. The ratio of sur3 colonies to wild-type colonies was 1:1 at 0 hours and 5:1 at 24 hours. The M. tuberculosis ORFs responsible for increased survival were shown to be Rv0365c for the sur2 clone and Rv2235 for the sur3 clone. These ORFs encode proteins with as-of-yet unknown functions.
We identified two M. tuberculosis ORFs which may be involved in the ability of tubercle bacilli to survive in macrophages.
Today, an estimated one-third of the world's population is infected with Mycobacterium tuberculosis, the causative agent of tuberculosis [1, 2], and tuberculosis causes about 2 million deaths annually [1, 2]. Many aspects of the interactions between M. tuberculosis and its human host remain unclear. Not only is this bacterium able to evade the defenses of the host's immune system, it is also able to persist in the body for years and may reactivate to cause disease decades after the initial infection. A better understanding of the interaction between M. tuberculosis and its human host is critical to developing new strategies to control the tuberculosis epidemic.
A key feature of the pathogenicity of M. tuberculosis is its ability to evade the antimicrobial processes of the macrophage and replicate intracellularly. Mycobacteria enter macrophages primarily by conventional receptor-mediated phagocytic pathways . Following phagocytosis, phagosomes containing viable tubercle bacilli fail to acidify, apparently because of failure to insert a proton-ATPase pump into the phagosomal membrane [4–6]. This leads to altered vacuolar maturation such that phagosome-lysosome fusion is blocked and the mycobacteria-containing vacuoles end up with an internal pH of about 6 and markers of phagosomes and early and late endosomes [7–11]. The mycobacterial genes responsible for these processes are largely unknown.
One method for identifying bacterial genes involved in pathogenesis is to express these genes in a nonpathogenic host and isolate bacteria with increased virulence. This technique was first used to identify a gene that enables Yersinia pseudotuberculosis to invade HEp-2 cells . In these studies, Escherichia coli bacteria expressing Y. pseudotuberculosis genes were used to infect HEp-2 cells. Only E. coli bacteria expressing the Y. pseudotuberculosis inv gene were able to invade the animal cells . Using a similar approach, Arruda et al. identified an M tuberculosis gene responsible for invasion of HeLa cells. We have previously used a similar technique to identify M. leprae genes involved in intracellular survival . That is, by expressing M. leprae genes in E. coli, we were able to isolate recombinant bacteria that exhibited increased resistance to killing by murine bone-marrow derived macrophages .
A similar approach was used by Wei et al  to isolate 21 Mycobacterium smegmatis recombinant clones that displayed a greater than 2-fold enhancement in survival after 48 hours. M. smegmatis is a fast-growing, nonpathogenic species of Mycobacterium in which M. tuberculosis genes can be efficiently expressed [16–18]. An in depth analysis of one recombinant clone revealed that M. smegmatis recipients carrying the M. tuberculosis eis gene on an extrachromosomal multicopy plasmid displayed 2.4- to 5.3-fold greater survival in U937 macrophages than wild-type M. smegmatis bacteria at 24 to 48 hours post-infection . The 42-kDa eis gene product has been shown to be associated with the mycobacterial cell surface and is released into extracellular medium, but its precise function is not yet known .
In the studies reported here, we used a similar enrichment scheme to identify two additional recombinant M. smegmatis clones, sur2 and sur3, that demonstrated enhanced survival during infections of THP-1 human monocyte-derived macrophages. The M. tuberculosis ORFs responsible for increased survival were shown to be Rv0365c for the sur2 clone and Rv2235 for the sur3 clone. These ORFs encode proteins with as-of-yet unknown functions.
Cosmids carrying M. tuberculosis genes  were electroporated into M. smegmatis LR222 to create a library of M. smegmatis transformants, each of which carries a pYUB178::H37Rv cosmid integrated into its chromosome. The library was generated from about 4000 independent transformants. This represents about 20 M. tuberculosis genome-equivalents given that ~225 cosmids contain one genome-equivalent of M. tuberculosis.
To compare directly the relative ability of the sur2 and wild-type bacteria to survive in macrophages, THP-1 macrophages were infected with a mixture of a genetically marked control strain and the sur2 strain and the survival of each strain was followed independently as previously described . The wild-type strain expresses the xylE gene product, catechol 2,3-dioxygenase, such that when its colonies are sprayed with catechol, they turn bright yellow, while wild-type colonies remain white. In essence, each well of the experiment contains an internal standard (the wild-type bacteria) to which to compare the survival of the recombinant bacteria. In experiments in which THP-1 cells were infected at a MOI of 50:1 (results in ~1 phagocytosed M. smegmatis bacterium per macrophage) with a mixture containing equal numbers of bacteria of the xylE-expressing control strain and a strain carrying the cosmid vector pYUB178, the ratio of recovered white colonies to yellow colonies was 1:1 at all time points (data not shown). This indicates that the survival of the xylE-expressing strain was the same as that of the wild-type and could be used an internal reference by which to measure the survival of other clones.
Comparison of the sequence of the cloned 1.1 kb fragment with the M. tuberculosis H37Rv genome sequence  revealed that it contains portions of the Rv0366c and Rv0365c genes (Figure 4). In the sur2 clone, the amino-terminal 970 bp of the 1128 bp Rv0365c ORF is fused to 14 bp from the pYUB178 vector to generate an ORF encoding 328 amino acids (aa), compared to 376 aa encoded by the full-length Rv0365c ORF. The insert also contains 104 bp of the Rv0366c ORF fused to 220 bp of the L5 integrase ORF. This ORF could encode a 108 aa protein which contains the 73 amino-terminal amino acids of the 344 aa L5 integrase protein fused to the 34 carboxyl-terminal amino acids of the Rv0366c ORF.
To investigate the difference in survival at 24 hours of the full-length Rv0365c ORF expressing bacteria and sur2 bacteria, a strain expressing Rv0365c and xylE was constructed and used in THP-1 coinfections with the original sur2 clone. In this coinfection, the ratio of white (sur2) to yellow (xylE and Rv0365c expressing) colonies remained 1:1 through 12 hours and then increased to ~3:1 at 24 hrs, consistent with the above-described observations.
A PCR fragment containing the M. tuberculosis ORFs Rv2233, Rv2234, and Rv2235 without the upstream promoter region, was cloned into the pBPhin vector, which does not contain a promoter to express the inserted DNA. In coinfections with the xylE-expressing bacteria, the recombinant bacteria containing Rv2233, Rv2234, and Rv2235 did not exhibit the same increase in macrophage survival as the sur3 clone (data not shown), suggesting that the Rv2235 ORF is expressed using signals upstream of the Rv2233 ORF.
Southern blots of genomic DNA from M. tuberculosis, M. smegmatis, Mycobacterium leprae, and Mycobacterium avium were probed with Rv0365c and Rv2235. Rv0365c hybridized to bands in M. tuberculosis, M. avium, and M. smegmatis under high stringency conditions (data not shown). Rv2235 hybridized to a band in M. tuberculosis, and under low stringency conditions recognized a band in M. leprae (data not shown).
Database searches revealed that Rv0365c encodes an ~41-kDa protein which displays significant homology only to a truncated Corynebacterium glutamicum hypothetical protein of unknown function (42% identity in a 296 aa overlap) located upstream of a gene encoding a fructose-bisphosphate aldolase. Rv2235 encodes a conserved hypothetical membrane protein of about 30-kDa and shares a motif with the SURF-1 family of proteins. The other two ORFs in the putative Rv2233-Rv2235 operon, Rv2233 and Rv2234, share homology with several proteins in data base searches. Rv2233 may encode a putative phosphatase, and Rv2234 may encode a low molecular weight protein tyrosine phosphatase .
There are several potential limitations of isolating M. tuberculosis genes involved in intracellular survival using an enrichment procedure. One limitation is that this protocol is biased towards the recovery of clones with the greatest increase in survival relative to wild-type . Thus, the recovered clones are not a random collection of genes involved in intracellular survival and hence the number of genes involved for survival cannot be calculated. This approach is also biased towards identifying genes expressing proteins that directly interfere with the antimicrobial processes of the macrophage. Some types of genes involved in resistance to killing, such as genes that are part of a multi-enzyme pathway, may not be isolated. Also, genes necessary for intracellular survival as opposed to resistance to killing are not likely to be isolated.
Two general classes of clones might be recovered following enrichment of the M. smegmatis recombinant library for clones with increased intracellular survival. One type might be clones that carry M. tuberculosis genes that confer enhanced resistance to the antimicrobial processes of the macrophage. Another type might be clones whose M. tuberculosis gene products are involved in attachment or invasion or increase phagocytosis. The two genes that were isolated in the studies reported here confer enhanced resistance rather than increased uptake. That is, the ratios of bacteria expressing either Rv0365c or Rv2235 to wild-type bacteria were 1:1 in both the initial mixture and inside the macrophages at the end of the phagocytosis period. Differences in survival compared to wild-type did not become apparent until about 9 hours post-phagocytosis for bacteria expressing Rv0365c and about 6 hours post-phagocytosis for those expressing Rv2235.
In this study, the enrichment process resulted in the isolation of two strains containing small, integrated plasmids rather than the expected 30–50 kb cosmids . Small plasmids corresponding to the integrated ones were present in the original E. coli (pYUB178:H37Rv) cosmid library. PCR, sequencing, and Southern blot analysis demonstrated that the plasmid integrated in the sur2 clone was approximately 4 kb containing 1.1 kb of M. tuberculosis DNA and the plasmid integrated in the sur3 clone was approximately 10.7 kb containing about 5.7 kb of M. tuberculosis DNA. Cosmid libraries frequently contain clones without DNA inserts , so it is not too surprising that clones with small DNA fragments were present in the E. coli library after infection with λ phage. The small plasmids may have a growth or replication advantage causing them to be over-represented in the library following the various amplification steps.
The ~2 kb deletion of vector DNA in the sur2 clone results in a fusion of the ORF of the L5 integrase with that of the M. tuberculosis insert such that the fused ORF encodes a protein that contains only the amino-terminal 73 aa of the 344 aa L5 integrase. The observation that the sur2 clone contains an integrated plasmid corresponding to the plasmid isolated from the cosmid library suggests that either a) the hybrid protein retains integrase activity, b) the plasmid inserted into the attachment site by homologous recombination between attP and attB, or c) a functional integrase was provided in trans by a second cosmid transiently present in the original transformant or by a gene present in the M. smegmatis LR222 genome. However, repeated attempts to electroporate the 4 kb plasmid into M. smegmatis LR222 did not generate any stable kanamycin-resistant transformants (data not shown). These results suggests that the hybrid protein does not retain integrase activity, that integration by homologous recombination into attP is unlikely, and that provision of integrase activity by an M. smegmatis gene chromosomal does not occur. The simplest explanation is that integrase was provided in trans by a second cosmid transiently present in the original transformant, and indeed, M. smegmatis bacteria carrying an integrated copy of the 4-kb plasmid can be readily isolated following electroporation of a mixture of the 4-kb plasmid and a plasmid that expresses integrase (unpublished results).
Data base searches did not reveal any homologies that could be used to predict functions for the gene products of ORFs Rv0365c or Rv2235. Rv0365c encodes a hypothetical protein of 376 amino acids which displays significant homology only to a Corynebacterium glutamicum hypothetical protein of unknown function (42% identity in a 296 aa overlap) . Rv2235 encodes a hypothetical protein of 271 aa with three putative transmembrane domains and which displays significant homology only to hypothetical protein MLCB1243.32c in M. leprae, to which it is 74% identical [23, 25]. This M. leprae homologue was evident in Southern blot experiments done under low stringency conditions. Rv2235 also contains the SURF-1 signature sequence  and modest homology to members of the SURF-1 family such as the SURF-1 protein of Caulobacter crescentus (25% identity; 40% similarity) . SURF-1 proteins are ~33-kDa, integral membrane proteins whose precise function is not known. In eukaryotic cells, SURF-1 proteins are involved in the assembly and maintenance of mitochondrial respiratory chain complexes including cytochrome oxidase [26, 28]. The homology to SURF-1 proteins raises the possibility that Rv2235 could play a role in resisting the antimicrobial activities of macrophages by helping to maintain the stability or function of an important cellular process, akin to the stabilizing role of chaperonins during a heat shock.
In addition to the genes described in this report, other studies with M. smegmatis recombinants and/or mutants have implicated 11 other M. tuberculosis genes in intracellular survival. Genes identified using enrichment or screening protocols include Rv2962c and Rv2958c (probable glucuronsyl transferases), Rv2220 (glutamine synthetase A1), Rv3913-Rv3914 (thioredoxin, thioredoxin reductase) and Rv2416c (eis, unknown function) [14, 15, 22, 29]. By screening insertional mutants of M. smegmatis, Lagier et al  isolated 8 mutants with impaired ability to survive in human peripheral blood monocyte-derived macrophages and identified the M. tuberculosis gene corresponding the mutated M. smegmatis gene for five of them. The genes included: Rv3052c (probable nrdI) which is postulated to be involved in deoxynucleotide production under stressed conditions; Rv0101 which is a nonribosomal peptide synthetase that displays strong homology with a Pseudomonas nonribosomal peptide synthetase required for the synthesis of the pyoveridine, a siderophore involved in iron uptake; Rv3420c which displays homology with the S18 ribosomal protein acetyltransferase which behaves as a heat shock protein in Chlamydia trachomatis; and Rv0497 and Rv3604c which are hypothetical conserved membrane proteins of unknown function.
It should be noted that these M. tuberculosis genes generally confer only a limited enhancement of the survival of M. smegmatis bacteria in the human macrophages. That is, usually only a few per cent of the recipients are viable 24 to 48 hours post-infection. This reinforces the concept that the intracellular survival of a pathogenic mycobacteria is a complex multifactoral process. The precise role(s) of any of the identified genes in the intracellular survival of mycobacteria is not yet known, although several of the identified genes have features of stress response genes. This is not unexpected given the relatively small enhancements of the survival of the M. smegmatis recombinants and the numerous environmental stresses encountered in the macrophage. Additional studies, such as the construction and characterization of targeted knock-out mutants, will be needed to determine the roles of the proteins encoded by these genes in the survival of M. tuberculosis in human macrophages.
Using an enrichment and screening procedure, two M. tuberculosis genes, Rv0365c and Rv2235, were identified that could confer an enhanced ability to survive in human macrophages to normally susceptible M. smegmatis recipients. The functions of these two proteins are not known. This study brings the number of M. tuberculosis genetic loci that have been implicated in enhancing the intracellular survival of M. smegmatis cells to 13. The precise role(s) of any of the identified genes in the intracellular survival of mycobacteria remain to be elucidated.
Materials and methods
Bacterial strains, plasmids, and growth conditions
Bacterial strains and plasmids used in this study.
E. coli XL 1 -Blue
M. smegmatis LR222
M. smegmatis LR222 (pYUB178)
M. smegmatis LR222 (pHIP1)
M. smegmatis LR222 (pHIP2)
M. smegmatis LR222 (pHIP3)
M. smegmatis LR222 (pHIP4)
M. smegmatis LR222 (pHIP5)
M. smegmatis LR222 (pHIP6)
M. smegmatis LR222 (pHIP7)
M. smegmatis LR222 (pHIP8)
M. smegmatis LR222 (pHIP9)
M. smegmatis LR222 (pHIP10)
M. smegmatis LR222 (pHIP11)
M. smegmatis LR222 (pHIP12)
M. smegmatis LR222 (pHIP13)
Rv2233, Rv2234, Rv2235
M. smegmatis LR222 (pHIP14)
Rv2238c, Rv2239c, Rv2240c
M. smegmatis LR222 (pBPhin1)
M. smegmatis LR222 (pBPhin2)
Rv2233, Rv2234, Rv2235
integrating, hsp65 promoter
The E. coli (pYUB178::H37Rv) library was grown in Luria broth (LB) (Difco Laboratories, Detroit, Mich.) containing 50 μg kanamycin/mL (Sigma Chemical Company, St. Louis, Mo.). The M. smegmatis (pYUB178::H37Rv) library was grown in Middlebrook 7H9 media (Difco) containing 10 μg kanamycin/mL and 0.05% (v/v) Tween 80 (Sigma) or on tryptic soy agar (TSA) (Difco) containing 10 μg kanamycin/mL. E. coli bacteria containing pHIP-based plasmids were grown in LB containing 200 μg hygromycin/mL (Boehringer Mannheim Biochemicals, Indianapolis, Ind.). M. smegmatis bacteria containing pHIP-based plasmids were grown on TSA containing 50 μg hygromycin/mL or in Middlebrook 7H9 media containing 50 μg hygromycin/mL and 0.05% (v/v) Tween 80.
An amplicon containing the hsp65 promoter was generated by PCR from the M. tuberculosis H37Rv genome. This PCR fragment was cloned into the Bam HI site of pBPhin  to generate pHIP. An amplicon carrying the complete xylE ORF without the xylE promoter was generated by PCR of pTKmx , cleaved with Bam HI, and cloned into the Bam HI site of pHIP downstream from the hsp65 promoter to generate pHIP1.
All enzyme reactions were performed as recommended by the manufacturers (GIBCO Bethesda Research Laboratories, Inc., Gaithersburg, Md., New England Biolabs, Beverly, Mass.). Cosmid DNA from E. coli was prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Valencia, Calif.) according to manufacturer's instructions for low-copy number plasmids. Wizard Plus Minipreps Kits (Promega, Madison, Wis.) were used to isolate plasmid DNA from E. coli strains. Mycobacterial genomic DNA was isolated as previously described .
PCR and sequencing primers used in this study. Underlined bases are restriction enzyme sites used in cloning. Bold bases represent either the start codon or stop codon of the gene being cloned.
Primer Sequence (5' to 3')
5' end of HSP65 promoter
3' end of HSP65 promoter
5' end of xylE
3' end of xylE
5' end of sur2 probe
3' end of sur2 probe
5' end of Rv0365c
3' end of Rv0365c
sur2 end of truncated Rv0365c
5' end of HSP65 promoter
3' end of Rv0365c
5' end of Rv0365c promoter
5' end of Rv2233
3' end of Rv2233
5' end of Rv2233
5' end of Rv2234
3' end of Rv2234
5' end of Rv2235
3' end of Rv2235
3' end of Rv2235
5' end of Rv2236c
3' end of Rv2236c
5' end of Rv2237
3' end of Rv2237
5' end of Rv2238c
3' end of Rv2238c
5' end of Rv2239c
3' end of Rv2239c
5' end of Rv2240c
3' end of Rv2240c
THP-1 human macrophages
The THP-1 cell line was obtained through the Biological Products Branch of the National Center for Infectious Diseases, CDC. THP-1 cells were grown in RPMI 1640 media (GIBCO BRL) containing 10% fetal calf serum (FCS) (GIBCO BRL) at 37°C in 5% CO2. To differentiate the THP-1 cells into macrophage-like cells, the cells were treated with 10 μM phorbol myristate acetate (PMA) (Sigma) as follows . All PMA manipulations were done under low light conditions. THP-1 cells were harvested by centrifugation for 10 minutes at 228 × g, and the pellet was resuspended in RPMI 1640/10% FCS/10 μM PMA to give a cell density of approximately 1 × 106 THP-1 cells/mL. Three milliliters of the suspension was added to each well of a six-well tissue culture plate (Costar, Corning, NY). The plates were incubated for 48 hours at 37°C in 5% CO2. The medium was removed from each well, the attached cells were washed once with RPMI 1640/10% FCS, and 3 ml of fresh RPMI 1640/10% FCS was added. The cultures were incubated at 37°C in 5% CO2 for an additional 48 hours. Immediately prior to infection, cells in each well were washed once with fresh RPMI 1640/10% FCS.
About 106 bacteria of the M. smegmatis (pYUB178::H37Rv) library were inoculated into Middlebrook 7H9 media containing 10 μg kanamycin/mL and then were grown to midlog phase (OD600 ~ 0.3). The bacteria were harvested by centrifugation for 1 minute at 16,000 × g and washed twice with RPMI 1640/10% FCS. The bacteria were suspended in RPMI 1640/10% FCS at 5 × 108 bacteria/mL. The enrichment process was as follows (Figure 1): differentiated THP-1 macrophages were infected by adding 3 mL of the bacterial suspension to each well of a six-well plate. The multiplicity of infection (MOI) was ~500 bacteria per THP-1 cell. The culture was left at 37°C in 5% CO2 for 2 hours, which resulted in the phagocytosis of about 10 bacteria per macrophage. After the phagocytosis period, each well was washed twice with RPMI 1640/10% FCS to remove free bacteria. To kill any remaining extracellular bacteria, 3 mL of fresh RPMI 1640/10% FCS/200 μg amikacin/mL (Sigma) was added to each well. The infected THP-1 cultures were incubated at 37°C for an additional 5 hours. Each well was then washed twice with 3 mL of RPMI 1640/10% FCS, and then 1 mL of 0.1% (v/v) Triton X-100 (Sigma) was added to each well to lyse the macrophages. The wells were scraped with a rubber policeman, and the lysates were removed and diluted for plating on TSA containing 10 μg kanamycin/mL. After 3 days of incubation at 37°C, bacteria from the resulting colonies were harvested, suspended in Middlebrook 7H9 media containing 10 μg kanamycin/mL and 0.05% (v/v) Tween 80, and pooled for reinfection of THP-1 macrophages. The enrichment process was done for a total of three cycles. After the third round of enrichment, individual clones were isolated and analyzed.
Pst I-digested genomic DNA was electrophoresed through a 1.0% agarose gel, denatured, neutralized, and transferred by capillary blotting to a Hybond -N+ membrane (Amersham, Arlington Heights, IL). The blots were hybridized to pYUB178 plasmid DNA labeled using the ECL Direct Nucleic Acid Labeling and Detection System (Amersham). All hybridization and washing steps were done at 42°C under either stringent (0.1 M NaCl) or non-stringent conditions (0.5 M NaCl) according to kit instructions.
Coinfection assay to measure survival
Coinfection assays were done as previously described . Briefly, separate cultures of recombinant and of xylE-expressing bacteria were grown to midlog phase (OD600 = ~0.3). The bacteria from each culture were harvested by centrifugation for 1 minute at 16,000 × g, washed twice with RPMI 1640/10% FCS, and resuspended in RPMI 1640/10% FCS at a concentration of 1.5 × 108 bacteria/mL. Equal volumes of the two bacterial suspensions were mixed to produce a suspension containing a 1:1 ratio of recombinant-to-control bacteria. A portion of the combined mixture was plated onto TSA plates to determine the number of colony forming units (CFUs) of each strain in the initial inoculum (the -2 hour time point in figures). The bacterial suspension was diluted with RPMI 1640/10% FCS to give approximately 5 × 107 bacteria/mL, and 3 mL was added to each well containing 1 × 106 THP-1 macrophages (MOI of 50 bacteria/macrophage). The cultures were incubated for 2 hours at 37°C in 5% CO2 to allow phagocytosis to occur, and then each well was washed twice with RPMI 1640/10% FCS to remove unphagocytosed bacteria. Typically, this results in one phagocytosed bacterium per macrophage. To kill extracellular bacteria, 3 mL of RPMI 1640/10% FCS containing 200 μg amikacin/mL was added to each well. Cultures were incubated at 37°C in 5% CO2. At various times, the medium was removed from each of three wells, and 1 mL of 0.1% (v/v) Triton X-100 in H20 was added to each well to lyse the macrophages. Each lysate was diluted as necessary, and portions were plated on TSA plates. The cultures which were assayed immediately after the addition of the media with amikacin serve to measure of the number of phagocytosed viable bacteria; the time at which these cultures were assayed was considered time zero (t0).
After a 3-day incubation at 37°C, the TSA plates from each time point were stored overnight at 4°C. The following day, the plates were sprayed with 0.5 M catechol (Sigma) in 50 mM potassium phosphate (pH 7.5) to distinguish the xylE-expressing colonies (yellow) from the recombinant colonies (white). Storing the plates overnight at 4°C results in a stronger yellow color. To determine the percent survival of a particular clone at time point X, the number of CFUs at time × was divided by the number of CFUs at to and multiplied by 100.
Recovery of the cloned M. tuberculosis genomic DNA fragment
One μg of sur2 genomic DNA was digested with the restriction enzyme Pst I then ethanol precipitated. The precipitated DNA was resuspended in 20 μL T4 ligase buffer (GIBCO BRL) and then ligated for 2 hours at room temperature with 0.5 units of T4 ligase (GIBCO BRL). The ligation mixture was electroporated into electrocompetent E. coli, and kanamycin-resistant transformants were isolated. After sequencing the M. tuberculosis H37Rv insert in the recovered plasmid, PCR primers were designed to amplify a product containing a portion of the cloned M. tuberculosis H37Rv genomic DNA. The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.
A plasmid carrying a portion of the M. tuberculosis H37Rv insert in the sur3 clone was isolated in a similar manner from a Bam HI digest of sur3 genomic DNA. The Bam HI/Eco RI fragment of the M. tuberculosis H37Rv insert of the recovered plasmid was used as a probe in colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library.
Portions of the E. coli (pYUB178::H37Rv) cosmid library were plated on LB agar containing 50 μg kanamycin/mL, and colony blots were performed with the ECL Direct Nucleic Acid Labeling & Detection System (Amersham) according to manufacturer's instructions. Colonies hybridizing with the probe of interest were removed as plugs and incubated in LB containing 50 μg kanamycin/mL for approximately 30 minutes at 37°C. Dilutions of this culture were plated on LB agar containing 50 μg kanamycin/mL to give well-separated colonies. Colony blots were performed and positive clones were selected for further study.
Subcloning ORFs into pHIP and pBPhin
Unless otherwise stated, all M. tuberculosis open-reading frames (ORFs) were generated by PCR from M. tuberculosis H37Rv genomic DNA as full-length ORFs without their natural promoters. The PCR primers (Table 2) were designed to contain restriction enzyme sites for cloning of the amplicon into the pHIP vector downstream of the hsp65 promoter as well as the eight basepairs (bp) upstream of the start codon of the gene being cloned. Because the hsp65 promoter in pHIP contains a ribosome binding site (rbs), the spacing between the rbs and the start codon of the cloned gene is about the same in these constructs as the spacing between the rbs and the start codon of the hsp65 gene in wild-type M. tuberculosis.
A truncated form of the Rv0365c gene was generated by PCR to contain 969 bp of the 970 bp of the truncated ORF present in the sur2 clone followed immediately by a stop codon. This fragment was Bam HI-digested and ligated to Bam HI-digested pHIP to form pHIP3. An amplicon containing the hsp65 promoter and full-length Rv0365c gene was generated by PCR amplification of the pHIP2 plasmid. This amplicon was Not I-digested and cloned into the Not I site of the pHIP1 plasmid containing the xylE gene to generate pHIP4. Plasmid pBPhin1 was constructed by ligating a Bam HI-digested PCR fragment containing the Rv0365c gene plus 24 bp located upstream of it to Bam HI-digested pBPhin. Plasmid pHIP13 was created by cloning a Bgl II-digested PCR fragment containing Rv2233,Rv2234, and Rv2235 into the Bam HI site downstream of the hsp65 promoter of pHIP. Plasmid pHIP14 was created by cloning a Bam HI- digested PCR fragment containing Rv2238c, Rv2239c, and Rv2240c into the Bam HI site downstream of the hsp65 promoter of pHIP such that Rv2240c was proximal to the hsp65 promoter. Plasmid pBPhin2 was created by ligating the Bgl II-digested Rv2233-Rv2235 fragment into the Bam HI site of pBPhin.
All electroporations were conducted using a Bio-Rad Pulse Controller (Bio-Rad, Hercules, Calif). Preparation and electroporation of competent E. coli XL 1-Blue cells were done according to Bio-Rad instructions. Competent M. smegmatis LR222 cells were prepared and electroporated as described by Jacobs et al..
All sequencing reactions were prepared with the Applied Biosystems, Inc. (ABI) PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) according to the manufacturer. All sequencing was conducted using an ABI 373 DNA Sequencing System (Applied Biosystems).
Results were analyzed by the two-sample T test.
DNA and protein homology analyses
DNA and protein database searches were performed using the BLAST services (blastn, blastp, and psi-blast) at the National Center for Biotechnology Information (NCBI) [35, 36] and the genomes site at The Institute for Genomic Research http://www.tigr.org. Protein domain/motif searches were performed using PSI-BLAST , and CD-Search (RPS-BLAST)  at NCBI and the SMART utility at the European Molecular Biology Laboratories [38, 39].
We thank Dr. William Jacobs for generously providing the pYUB178::H37Rv cosmid library and Dr Jack Crawford for providing strain LR222.
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