Survival of M. smegmatis LR222 bacteria expressing ORFs in the sur3 insert. THP-1 macrophages were infected with an equal mixture of xylE-expressing bacteria and bacteria expressing one or more of the sur3 ORFs. The ratio of the recovered white-to-yellow colonies is shown for -2 hours (initial inoculum), 0 hours (immediately after phagocytosis interval), and at 6 and 12 hours after phagocytosis. THP-1 macrophages were infected with an equal mixture of xylE-expressing bacteria and bacteria expressing Rv2233 (first open column), Rv2234 (first /// lines), Rv2235 (first \\\ lines), Rv2236c (hatched), Rv2237 (horizontal stripes), Rv2238c (vertical stripes), Rv2239c (box pattern), Rv2240c (second open column), Rv2233-Rv2235 (second /// lines), or Rv2238c-Rv2240c (second \\\ lines). Error bars represent the standard deviation in the ratio of white-to-yellow colonies between experiments.