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Table 2 PCR and sequencing primers used in this study. Underlined bases are restriction enzyme sites used in cloning. Bold bases represent either the start codon or stop codon of the gene being cloned.

From: Identification of two Mycobacterium tuberculosis H37Rv ORFs involved in resistance to killing by human macrophages

Primer Sequence (5' to 3')

Location/ Function

GGATAGATCTAGTTGCTGCAGCGT

5' end of HSP65 promoter

GAAGTGGATCCTCCGATCGGGGATG

3' end of HSP65 promoter

GACGGATCCATG ACGTCATGAAC

5' end of xylE

GACGGATCCAAGCTTGCATGCC

3' end of xylE

CGGTGTCGCGGGCGGCGGCGTCG

5' end of sur2 probe

GATCGCATTATGAATCTGGCAAACC

3' end of sur2 probe

CAGGGATCCATCGCATTATG AATCTGGCAAACCG

5' end of Rv0365c

CAGGGATCCGTGTTA CCCGAGTGAGCTGACC

3' end of Rv0365c

CAGGGATCCTTA ATCCCAGAACGCCCCGAACACC

sur2 end of truncated Rv0365c

GCGGCCGCAGATCTAGTTGCTGCAGCGTGAC

5' end of HSP65 promoter

GCGGCCGCGTGTTACCCGAGTGAGCTGACC

3' end of Rv0365c

GGATCCGACAGGCTAGGGCAGGATCGC

5' end of Rv0365c promoter

GACGGATCCGTTGGGCGATG AAACAGCTTG

5' end of Rv2233

GACGGATCCGGATCA GACACCTAGCGCCTC

3' end of Rv2233

GACAGATCTGTTGGGCGATG AAACAGCTTG

5' end of Rv2233

GACAGATCTGGCGCTAGGTG TCTGATCCGC

5' end of Rv2234

GACAGATCTGCATCA ACTCGGTCCGTTCCG

3' end of Rv2234

GACGGATCCCCGAGTTGATG CCCCCGCC

5' end of Rv2235

GACGGATCCGGTTTA CCGCCGGCGGCC

3' end of Rv2235

GACAGATCTGGTTTA CCGCCGGCGGCC

3' end of Rv2235

GACGGATCCCTGCCGGAGTG TTTGCATCG

5' end of Rv2236c

GACGGATCCCCGCTA CGGCCGCCGGC

3' end of Rv2236c

GACGGATCCCCAGGTCGATG CAAACACTCC

5' end of Rv2237

GACGGATCCGCTCTA TCAGACGATTCGGCG

3' end of Rv2237

GAGGGATCCAAGCGCTGATG CTGAACGTCG

5' end of Rv2238c

GAGGGATCCCCCAAAACCTTA GGCCGTAAG

3' end of Rv2238c

GACGGATCCCGACGATAATG CCCATCGCG

5' end of Rv2239c

GACGGATCCGCATCA GCGCTTGCCAGCC

3' end of Rv2239c

GACGGATCCGCCAGATCGTG GCGGGCG

5' end of Rv2240c

GACGGATCCGCCTCA GAAGGCGGCCACG

3' end of Rv2240c