Map of the M. tuberculosis H37Rv DNA integrated in the sur3 genome. The integrated 10.7 kb cosmid is between the two attachment sites, attL and attR. The ~7.4 kb Bam Hl and ~4.5 kb Bam HI/Eco RI fragments are indicated by the Bam HI and Eco RI sites. The 5.76 kb fragment of M. tuberculosis H37Rv genomic DNA contains the 34 bp of DNA upstream of Rv2233, Rv2233-Rv2240c, and the tRNA for valine [Reference 23]. The arrows indicate the direction of transcription. Arrows are color coded according to annotations found on the TubercuList server http://genolist-Pasteur.fr/TubercuList/: light green (unknown), brown (conserved), yellow (intermediary metabolism), light blue (regulatory), gray (virulence), red (information pathway). dark green (cell wall process), black (lipid metabolism), and dark blue (stable RNA).