- Open Access
Potential use of beneficial fungal microorganisms and C-phycocyanin extract for enhancing seed germination, seedling growth and biochemical traits of Solanum lycopersicum L.
BMC Microbiology volume 22, Article number: 108 (2022)
Biopriming as a new technique of seed treatment involves the application of beneficial microorganisms on the seed surface to stimulate seed germination, plant growth, and protect the seed from soil and seed-borne pathogens. The present investigation was carried out on seed germination, seedling vigor and biochemical traits of one of the most important vegetable crops (Tomato, Solanum lycopersicum L.). The treatments comprised viz. T1: Non primed seeds (Control), T2: Hydropriming, T3: Biopriming with C-phycocyanin (C-PC) (Spirulina platensis extract), T4: Biopriming with Trichoderma asperellum, T5: Biopriming with T. viride, T6: Biopriming with Beauveria bassiana.
Extraction and purification of C-phycocyanin (C-PC) from the dry S. platensis powder using various methods was performed. The purity after dialyses was 0.49 and its ultimate purity (A620/A280) after ion-exchange chromatography was 4.64. The results on tomato seedlings revealed that the maximum germination percentage (100%), germination index (15.46 and 15.12), seedling length (10.67 cm), seedling dry weight (1.73 and 1.97 mg) and seedling length vigor index (1066.7) were recorded for tomato biopriming with T. viride, and B. bassiana (T5 and T6). Moreover, the quantitative estimation of total carbohydrates and total free amino acids contents in bioprimed tomato seedlings indicated a significantly higher amount with T. viride, followed by those bioprimed with T. asperellum, B. bassiana and C-PC extract.
Thus, our results indicated that biopriming of tomato seeds with beneficial fungal inoculants and C-PC was very effective. The most operative biostimulants were those bioprimed with T. viride and B. bassiana compared to other biostimulants (T. asperellum and C-PC). Therefore, to ensure sustainable agriculture, this study offers new possibilities for the biopriming application as an alternative and ecological management strategy to chemical treatment and provides a valuable basis for improving seed germination.
Agricultural practices are continually being modernized to keep up with the ever-changing environment, with the introduction of genetically modified crops, plant growth regulators, fungicides, fertilizers, pesticides and so on. Their advantages, however, come at a price: some are time-consuming and costly to implement, while others are regarded as detrimental to consumer health and the environment in the long run [1, 2]. As a result, scientists must devise strategies that improve agricultural yield while minimizing hazards. Plant growth regulators and biostimulants are gradually becoming the primary research fields among many scientific researchers to improve plant growth and development [3,4,5]. Additionally, the positive association and interaction of rhizo-competent microbes are frequently employed for plant bio fertilization and stress-induced damage mitigation . A plant biostimulant is a product that encourages plant nutrition processes independently of its nutrient content. The sole purpose of it is the enhancement one or more of the subsequent characteristics of the plant and its rhizosphere such as accessibility of restricted nutrients in soil or rhizosphere, nutrient use performance, tolerance to abiotic stress and quality characters [7, 8]. Moreover, the biostimulant stimulates the response to the environment, such as stress circumstances or phytopathogenic attack [9, 10]. Furthermore, it can as well help plants to grow and develop in a variety of ways from seed germination to maturity, including improving metabolism to improve yield and crop quality, interacting with nutrient assimilation and translocation, facilitating plant defense against adverse conditions, and so on . Also, Rouphael et al.  and Calvo et al.  stated that the stimulation of germination, seedlings and plant growth as well as crop productivity in response to plant biostimulants has been usually related to the action of signaling bioactive molecules in the primary and secondary metabolisms. To fully understand the biological role of biostimulants, it’s necessary to evaluate the subject plants’ growth stage and pattern, as well as their developmental reactions [1, 3]. As a result, seed germination, being a crucial step in the growth of a new plant, it reflects the plants’ lateral growth pattern, the fitness, survival, persistence and evolutionary potential of plants . Internal and external factors are heavily regulating the seed’s dormancy status and germination potential at this stage.
Microbial biopriming is an adaptive approach for improving a plant’s defensive capacity, resulting in enhanced resistance/stress tolerance and/or a more exacerbated defense response to stress-inducing circumstances before germination [13, 14]. It also effectively reduces the dependence on chemical fungicide for diseases management [6, 15]. In seed biopriming, seeds were coated with a variety of agriculturally significant microorganisms, resulting in quick and consistent seed colonization [14, 16]. However, if seeds are infected with undesired indigenous microorganisms, they may proliferate during priming and may reduce the survivability of beneficial microbes , hence disinfecting the seeds before priming is required [15, 16].
The stimulatory consequence of biostimulants, such as algal extracts and plant-growth promoting fungi (PGPF), on seed germination and seedlings growth has been previously documented [14, 18, 19]. A representative, cyanobacteria can be used to make useful plant products such as fertilizers that play an important role in sustainable agriculture, helping to increase soil fertility, crop development, and environmental quality [20, 21]. Vitamins, amino acids, polypeptides, phytohormones (gibberellins, auxins, cytokinins), antioxidants, and substances with antibacterial and antifungal effects can all be found in Spirulina platensis, which can be exploited as a rich source of macro, micronutrients and proteins such as phycobiliproteins for plants [20, 22]. One of the most important phycobilliproteins is C-phycocyanin (C-PC; derived from cyanobacteria like S. platensis) which has been used as a natural blue dye in commercial applications .
Makhaye et al.  estimated the influence of algal extract and biostimulant biopriming on the seed germination parameters such as germination percentage and germination index of Abelmoschus esculentus. Also, Zhang et al.  determined the effect of PGPF (Trichoderma longibrachiatum) on wheat seedlings’ growth and improvement under stress, besides examining the role of T. longibrachiatum in inducing the resistance at physiological and biochemical levels. Trichoderma spp. rhizo-competent’s nature allows it to colonize roots, boost the plant immune system and have been explored as a possible biocontrol agent [25, 26]. Additionally, the colonization of these beneficial fungi promotes plant growth and protects the host plants from abiotic and biotic stressors . Moreover, Russo et al.  estimated the improving effect of endophytic fungus Beauveria bassiana on seed germination percentage of Zea mays.
One of the most important and extensively used vegetable crops is tomato plant (Solanum lycopersicum L.), it contains a variety of metabolites that have health and nutritional benefits. It also includes easy-to-maintain diploid genetics, a minimal generation time, and routine transformation technologies . Together these make tomato an excellent model plant for biologists for both basic and applied plant research. Tomato plants hampered with a load of pathogenic seed microflora that led to a number of nurseries such as seed rot and other field diseases. The infected seeds thus used are responsible not only for the poor germination seedlings stand but also for the carryover of pathogens to the field. Moreover, the germination time of tomato seeds is very high as compared to field crops which lead to non-uniform seedling stand and low vigor seedlings . Considering this, to ensure sustainable agriculture, biopriming is regarded as the most effective method of seed protection. Thus, the present study was conducted to extract and purify C-PC from the dry Spirulina powder using various methods and to evaluate its biological activity. Furthermore, the comparative effects of biopriming with fungal inoculums such as T. viride, T. asperellum, B. bassiana and C-PC on tomato seedling growth promotion was studied to consider their significant biological applications, in addition, to assess the hydrolytic enzymes activity and other biochemical attributes of tomato seedlings.
Results and discussion
Extraction and purification of C-PC
C-PC was extracted and purified in three steps: crude extract preparation (Step I), dialysis (Step II), and ion-exchange chromatography (Step III). The concentration and purity of C-PC were verified and improved with each purifying process (Table 1). The purity after dialysis was 0.49 and its ultimate purity (A620/A280) after ion-exchange chromatography was 4.64, where was eluted as a brilliant blue colored solution during the column chromatography. The purity of C-PC was evaluated at each fraction and increased about 9 times in the third fraction (highest purity) more than dialysis (Table 1). The highly pure C-PC’s absorption spectra revealed a strong peak at 615 nm (Fig. 1). Eriksen  documented that Spirulina is widely implemented as a high-quality protein for C-PC as a cyanobacterial accessory pigment with a variety of agricultural and industrial uses. A variety of publications on the extraction and purification of C-PC from cyanobacterial strains are available [31,32,33].
Extract purities not only vary per strain, but they are also influenced by the extraction methods used, and further purification techniques are frequently used to improve the purity of the extracts . Safaei et al.  employed a four-step purification procedure comprising the adsorption of impurities with chitosan, activated charcoal, ammonium sulfate precipitation, and ion-exchange chromatography, reaching a high purity form of C-PC of 5.26. Furthermore, Schipper et al.  discovered that the extraction buffer and cell disruption technique has an impact on the C-PC content and extract purity from Leptolyngbya sp. and Arthrospira platensis and reported that the cell disruption technique with CaCl2 was the best approach for A. platensis, while it was the second-best method for Leptolyngbya sp. In comparison to other approaches, Diethylaminoethyl (DEAE) column chromatography was considered to be an essential method for purifying C-PC from S. platensis according to Moovendhan et al. . However, Seo et al.  extracted C-PC from S. platensis using a hexane separation method and a high-pressure process.
FT-IR spectral analysis
In the current investigation, the C-PC of S. platensis revealed functional groups, with peak frequencies of 686.31 and 748.78 cm− 1 representing the presence of the C–H bond in the molecule. The CH2 bending vibration was identified at 1455 cm− 1, and the protein amide II band was detected at 1558 cm− 1 (C=O stretching). Furthermore, the presence of carboxylic acids, C = N and N–H bond in the molecule were shown at 2337.10, 1660 and 3205 cm− 1 respectively. These functional groups were recorded according to Gokel  (Fig. 2). Our findings are similar to those of Moovendhan et al. , who studied S. platensis C-PC and found functional groups at peak frequencies of 673.86, 794.67, 1456.26, 1539.20, and 2358.94, which are virtually identical to our data. The FT-IR spectrum of Ulva lactuca extract revealed C-PC as the most bioactive component, with transmittance maxima at 1652, whereas our results were 1660 cm− 1, which is mostly suggested by COO, CO, and conjugated double bonds. These bonds had spectral bands peaking at 2985 cm− 1, 2860 cm− 1, and 2986 cm− 1 , which corresponded to 2874 cm− 1 in our investigation.
1H NMR spectral analysis
In our investigation, 1H NMR spectra were measured at ppm level ranging from 21 to 13 ppm. The chemical shifts of C-PC signal 2.71 (δ), 2.87 (δ) and 3.76 (δ) confirmed the presence of Alkyne (C. C-H) type protons. Chemical shifts 6.86 (δ), 6.89 (δ), 6.92 and 6.98 (δ) proved the presence of an alkene with C-H type protons and N-H. Whereas chemical shift 7.3(δ) confirmed the presence of aromatic with H on the phenyl ring NH. Also, a pyrrolic NH signal was observed at 7.36 (Fig. 3). Wiegand et al.  used NMR spectroscopy to investigate the structural characteristics of phycoerythrocyanin peptides from thermophilic cyanobacterium Mastigocladus laminosus and Fischerella sp., and reported different functional groups with different proton types at various ppm and chemical shifts. Similarly, Moovendhan et al.  suggested the presence of 14 chemical shifts (δ) and confirmed the presence of alkyl halide, alkene and aldehyde proton in 1HNMR analysis of phycocyanin. With varying concentrations of pigments extracted from Chattonella verruculosa, Mangoni et al.  observed 19 and 6 chemical changes.
Germination and growth indices of tomato seedlings after seed biopriming
Seed biopriming improves the initial step of plant development by encouraging more uniform seed germination, inducing profound changes in plant characteristics and providing protection before seedling emergence . Figure 4(a-d) depicts the comparative effects of biopriming with fungal inoculums (T. viride, T. asperellum and B. bassiana) and C-PC extract on germination percentage (%), germination index (GI), seedling weight vigor index (SWFI) and seedling length vigor index (SLVI) of tomato seedlings after 12 days of growth. Also, the photograph was taken for S. lycopersicum seedlings to show the effect of the priming on the growth indices as compared to unprimed seedlings (Fig. 5). Likewise, the morphological data for tomato seedlings can be observed in Table 2. Concerning germination indices, an increase in SWVI in tomato seedlings primed with T. asperellum, T. viride and C-PC (160 and 173) and the maximum records were documented for the tomato seeds bioprimed with B. bassiana (197) compared to the unprimed or hydroprimed ones (125 and 137). Moreover, seed biopriming produced a staggering improvement in seedling’s FW, DW, shoot height and radicle length, where the highest values for the seedling FW were recorded for seedlings primed with T. viride (49.8 mg) and T. asperellum (48.8 mg). In contrast, the unprimed or hydroprimed seeds exhibited significantly (p < 0.05) lower records (19.9 and 28.9 mg). Our results are in harmony with Aamir et al.  who reported that the biopriming of tomato seeds with T. erinaceum caused a profuse growth in morphological attributes. Sánchez-Rodríguez et al.  recorded an enhancement in wheat growth colonized by B. bassiana. Moreover, Metwally and Al-Amri  and Metwally et al.  reported an increase in shoot length, root length, shoot and root FW and DW of onion with T. viride. Moreover, Russo et al.  reported that the percentage of corn seed germination was significantly increased with B. bassiana. As, Trichoderma spp. and B. bassiana are endophytic fungi, enhance plant growth by increasing nutrient uptake and production of plant growth regulators along with induction of secondary root development through indoles acetic acid (IAA), gibberellin, cytokinins and siderophores production . As well, these fungi produce phosphatase as well organic acids which solubilize the inaccessible phosphate to make it available, also help to increase the N2 use efficiency in plants [5, 44].
Similarly, the enhancement in both germination and growth indices of tomato seedlings primed with C-PC was in agreement with Muñoz-Rojas et al.  and Chua et al.  that the biopriming of Eucalyptus gamophylla, Senna notabilis and Acacia hilliana seeds with Microcoleus sp and Nostoc sp produced seedlings with a longer shoot and root lengths. Also, Haroun and Hussein  demonstrated an increase in growth indicators in Lupius termis treated with Cylindrospermum muscicola and Anabaena oryzae extracts. Essa et al.  recorded an elevation in the seed germination and the seedling growth criteria of Sorghum durra with Anabaena oryzae and Synechococcus sp. This elevation could be attributed to cyanobacteria’s bioactive compounds, minerals and trace elements, which have the ability to enhance the phytohormones levels and play a crucial role in plant growth regulation, metabolism, and development [46, 49].
Effects of seed biopriming on primary metabolites
Changes in the accumulation of the biomolecules show the real impact of treatments in the plants. To assess the effects of C-PC, T. viride, T. asperellum and B. bassiana on the accumulation of the primary metabolites, we measured total carbohydrates, protein and TFAA contents in tomato seedlings (Fig. 6a-c). Also, Pearson’s correlation was analyzed to demonstrate the relation between growth indices and primary metabolites; there were significant positive correlations between the seedling DW with protein (r = 0.856), carbohydrates (r = 0.825), and TFAA (r =0.766) (Table 3). From the quantitative estimation of Fig. 6, there were significantly (p < 0.05) higher amounts of total carbohydrates and TFAA contents in tomato seedlings bioprimed with T. viride (459.32 mg/g DW and 7.87 mg/g FW; respectively), followed by seedlings bioprimed with C-PC (401.31 mg/g DW and 7.14 mg/g FW) and B. bassiana (398.17 mg/g DW and 7.87 mg/g FW).
These results may highlight the promoting effect of fungal endophytes on the contents of these primary metabolites. Also, the results showed that the level of amino acids increased with a parallel increase in protease activity (Fig. 8) in all biopriming treatments. As, amino acids are derived from the degradation of intracellular proteins and their amount in plant tissues are carefully regulated to just meet the requirements for the biosynthesis of proteins .
Our findings of increasing the protein and carbohydrates contents are in agreement with Singh et al.  who recorded a significantly higher amount of total soluble sugar and proteins in the roots and leaves of Zea mays plants bioprimed with Pseudomonas aeruginosa. The enhancement of protein, TFAA and carbohydrates contents was also evident from increased plant growth parameters (Table 2). Macuphe et al.  revealed a significant increase in protein content of lettuce plants following endophyte colonization which is involved in carbohydrate metabolism, defense and photosynthesis [52, 53]. Since, proteins play an important role in the growth and nutritive value of plants and can mediate the production of antioxidants . Also, White and Torres  reported that plants colonized by endophytes produce more glucose and fructose.
Moreover, the C-PC application to tomato seeds has a positive effect on the protein (7.31 mg/g FW) (Fig. 6b), total carbohydrates (401.31 mg/g DW) (Fig. 6a) and TFAA levels (7.14 mg/g FW) (Fig. 6c). Our results were in coherence with Osman et al.  that Spirulina suspension can increase the creation of proteins and amino acids in roots and shoots of faba bean. Also, Haroun and Hussein  demonstrated an increase in nitrogenous chemical content and carbohydrates in shoots of L. termis treated with C. muscicola and Anabaena oryzae extracts. Mógor et al.  found that applying S. platensis lyophilized biomass, high in L-amino acids, stimulated red beet carbon metabolism and sugar content. Yakhin et al.  and Ana  documented that cyanobacteria release various kinds of biologically active substances like proteins, vitamins, carbohydrates, amino acids, polysaccharides and phytohormones, which function as signalling molecules to promote plant growth. Also, Nawrocka et al.  and Mógor et al.  found that the most essential amino acids, polyphenols, and vitamins such as tocopherol and ascorbic acid are abundant in C-PC from Spirulina extracts.
Effect of biopriming on secondary metabolites in tomato seedlings
Phenolic and flavonoid compounds are essential for plant functions due to their participation in defensive systems, plant tolerance to a variety of biotic and abiotic stresses, growth and development [59,60,61]. As well, shikimic acid is the precursor to a wide variety of secondary metabolites that play a key role in plant defense mechanisms . We measured the total phenolic, flavonoids and shikimic acid contents in tomato seedlings bioprimed with C-PC, T. viride, T. asperellum and B. bassiana. Our results (Fig. 7a-c) revealed a significant (p < 0.05) increase in their contents with priming. Also, in hydroprimed tomato seedlings (12.01 mg/g seedling FW), a significant (p < 0.05) effect on phenolic content was detected compared to unprimed ones (9.5 mg/g seedling FW) (Fig. 7b). Moreover, the highest shikimic acid and flavonoids contents were detected in seedlings primed with B. bassiana. These results may highlight the promoting effect of fungal endophytes on increasing these secondary metabolites contents in tomato seedlings. In agreement with our results, the aforementioned studies reported an increase in the accumulation of total flavonoids and total phenolic compounds following endophyte colonization in maize seedlings and tomato plants [52, 63]. Singh et al.  also reported an upsurge in shikimic acid content in chickpea leaves treated with triple microbe consortium. There are many plausible explanations for the higher production of secondary metabolites (alkaloids, terpenoids, flavonoids, and phenols). Perhaps maybe it is due to their direct production by the endophyte or the endophyte assists indirectly by influencing their production on the host plant . Moreover, Zaprometov and Nikolaeva  and Kovaleva et al.  demonstrated the role of polyphenols in regulating plant growth and development, since they affect the biosynthesis of indol-3-acetic acid which plays a key role in both root and shoot development. They also play an important role in the metabolism of plant cells, affecting different physiological processes such as cell division and expansion, membrane permeability, nutrient uptake, enzymatic activity and respiration . However, Moloinyane and Nchu  documented that B. bassiana did not have any significant effect on total polyphenol, alkaloid, and flavonoids in Grapevine plants. In another study, the total phenolic content of 15-day-old cotton seedlings was reported to be higher than that of 5-day-old seedlings after Cladorrhinum foecundissimum colonization . Also, an augmentation in their contents in tomato seedlings primed with C-PC extract was detected, Goiris et al.  linked phenolic compounds found in microalgae and cyanobacteria to antioxidant properties, and they play a significant role in growth, reproduction, and stress tolerance. Therefore, C-PC, T. viride, T. asperellum and B. bassiana not only promoted plant growth but also stimulated the accumulation of shikimic acid, phenolic and flavonoid contents in bioprimed seedlings.
Effects of seed biopriming on hydrolytic enzymes
During germination, the high molecular weight reserves in the storage organs of the seed are converted into transportable forms and are transported to metabolizing and growing tissues where they are utilized for energy-producing and synthetic events . The major types of these storage reserves in seeds are starch and proteins, where their conversion to transportable forms was accompanied by activation of hydrolytic enzymes such as amylases and proteases. Regarding to Table 3, the Pearson’s correlation showed strong positive correlations between growth indices and hydrolytic enzymes; shoot height exhibited a significant positive correlation with amylase (r = 0.815**) and protease (r = 0.799**). Our results (Fig. 8a and b) revealed an augmentation in the amylase and protease enzyme activities in tomato seedlings upon biopriming with T. asperellum, T. viride, B. bassiana and C-PC extract. The highest amylase activity (Fig. 8a) was recorded by B. bassiana (0.081 Change in OD/min) followed by seedlings bioprimed with T. viride (0.076) and C-PC extract (0.075 Change in OD/min) compared with hydropriming or control ones (0.047 Change in OD/min). This was in line with Robl et al.  that endophytic fungi such as T. atroviride, Alternaria sp., Annulohypoxylon stigyum and Talaromyces wortmannii are excellent producers of hydrolytic enzymes. These enzymatic activities are essential for providing energy and carbon skeletons to the growing embryo through the respiratory breakdown of utilizable substrates until the seedling becomes photo-synthetically self-sufficient.
Moreover, Caldwell et al.  reported the ability of root endophytic fungi, Philaophora finlandia and P. fortinii to produce hydrolytic enzymes, which were able to break down the major polymeric forms of C, N and P found in plants. Similar results were proved by Marlida et al.  and Maria et al. . In this connection, Gholam et al.  proved that the enhancement of seed germination by plant growth-promoting fungal inoculants was due to the synthesis of seed germination hormones like gibberellins which triggered the activity of specific enzymes, such as alpha-amylase. Furthermore, Mabood et al.  recommended that hydrolytic enzymes are of major interest due to their ability to degrade and lyse pathogen cell wall, and thus they are employed in biocontrol of fungal phytopathogens as Felse and Panda  reported in the control of Sclerotium rolfsii and F. oxysporum through the cell wall degradation by hydrolytic enzymes on beans.
Additionally, the enhancing effect of C-PC extract (Fig. 8) in germinated seedlings of tomato agreed with Osman et al.  who recorded an increase in amylases and proteases enzyme activities in germinated seedlings of pea treated with the cyanobacterial extract. Amylases increased the availability of starch assimilation by the hydrolysis of it into glucose . The other groups of enzymes are proteases which play an important role during germination, in the mobilization of stored protein in seed as free amino acids, which are utilized in building necessary protein and enzymes required for the growing embryo .
Seed biopriming is one of the innovative and ecofriendly priming methods, as it is useful not only for enhancing seed germination and seedling vigor, but also for the management of biotic and abiotic stresses. Our recent study tries to fill the gap by examining the comparative effects of these different biostimulants such as T. viride, T. asperellum and B. bassiana and C-PC extract on seed germination, seedling growth and biochemical traits of Solanum lycopersicum L. According to our results, the most effective biostimulants were those bioprimed with T. viride and B. bassiana as compared to other biostimulants (T. asperellum and C-PC). Thus, biopriming of seeds with the desired fungal endophytes can be used commercially as an alternative to biofertilizers successfully. Future strategies should apply biopriming with different biostimulants to other plant seeds yet not experimented which will give a better picture of the potential of this technology.
Preparation of priming materials
Fungal inoculums preparation
The cultures of T. viride, T. asperellum, B. bassiana were brought from Mycology Lab, Faculty of Science, Zagazig University. The fungal inoculums were prepared from 7-day old culture. In brief, the Petri dish containing the culture was suspended with sterile distilled water. The spores were gently removed using a glass spreader, and then the heterogeneous suspension was filtered using the muslin cloth for removing the mycelial mat. The filtered suspension was diluted with sterile distilled water and adjusted to 106–107 spores/ mL as quantified through the hemocytometer.
C-PC crude extract preparation
Growth and maintenance of culture
S. platensis was obtained kindly from Prof. Dr. Yassin El-Ayouty (Phycology Lab, Faculty of Science, Zagazig University). The culture was maintained in Z-Medium  at 28 ± 2 °C under a light intensity of 52–55 μEm−2s− 1and light/dark cycles of 16:8 h. At mid-logarithmic phase, algal cells were harvested by centrifugation at 10000 rpm (4 °C) for 15 min using Multi-tube under cooling centrifuge (Vision SCIENTIFIC CO., LTD., South Korea), washed three times with sterile distilled water, and air-dried.
Extraction and estimation of C-PC
About 10 g of dried algal cells were suspended in 50 mL of Calcium Chloride (10 gL− 1). Samples were subjected to freeze-thawing (incubated at − 20 °C until solid, followed by thawing for 24 h at 4 °C in the dark). Phycocyanin was extracted by repeated freezing (− 20 °C) and thawing at room temperature until the blue color becomes visible. Cell debris was removed by centrifugation at 5000 rpm for 10 min using Eppendorf under cooling centrifuge (MIKRO 200R Hettich zentrifugen, Germany) and the extract thus obtained was termed as a crude extract. The amount of C-PC was measured as described by Bennett and Bogard  and purity was determined by using the formulae: Purity = A620/A280. C-PC concentrations (μgPC L− 1) were determined according to Lawrenz et al. .
Purification of C-PC
The obtained crude C-PC was dialyzed against the extraction buffer using dialyses membrane (Dialysis membrane-70, MWCO; 12–14 kD) procured from Hi-Media. Dialysis was performed twice against a 1000 mL extraction buffer, first at room temperature and again dialyzed against 1000 mL of extraction buffer at 4 °C overnight. The resultant extract was recovered from the dialysis membrane and filtered through a 0.45 μm filter.
Ion exchange chromatography
Phycocyanin further purified by ion-exchange chromatography using a DEAE-Sepharose, from Enzymology and Fungal Biotechnology Lab (EFBL, Faculty of Science, Zagazig University). A column (2 × 30 cm) had been pre-equilibrated with 20 mM sodium acetate buffer containing 50 mM NaCl. After washing with 60 mL of the same buffer, the dialyzed filtered sample was placed on the column; the column was eluted with the same buffer. The elutes were collected in 5 mL fractions. Fractions were collected at a 0.5 mL/min flow rate . Then, the purity of all fractions was checked by equation. The absorption spectrum was also determined by scanning the highly purified sample in the range of 200–800 nm by using UV / VIS Spectrophotometer (T80, PG Instruments Ltd. (UK)).
Fourier-Transform InfraRed spectroscopy (FT-IR) spectral analysis
The functional group’s profile of the purified phycocyanin from S. platensis was done by FT-IR (Bruker, Germany) spectral analysis. The KBr pellet was prepared by mixing 1 mg of the sample with 100 mg of anhydrous potassium bromide. The spectra were recorded from 500 to 4000 cm− 1 and 30 scans at a resolution of 4 cm were averaged and referenced against air.
Nuclear Magnetic Resonance spectroscopy (1H NMR) spectral analysis
The structural feature of the purified phycocyanin from S. platensis was evaluated by 1H NMR spectra (Bruker, Germany) by following the method of Schanda and Brutscher . Approximately 30 mg of sample was dissolved in 0.5 mL of D2O (99.9%) in a NMR tube (5 mm diameter). The 1H NMR spectra were taken at 27 °C and the chemical shift was expressed in parts per million (ppm).
Priming, treatments and experimental conditions
Seeds of tomato (Solanum lycopersicum L.; Tomato HYBRID Seven F.1) were gained from the local market of Minia Al-Qamh, El-Sharkia Governorate. For seed biopriming with a spore suspension of T. viride, T. asperellum, B. bassiana and C-PC (S. platensis extract), the healthy seeds of tomato were surfaces sterilized with 2% (v/v) sodium hypochlorite (NaOCl) solution for 3 min followed by repeated washing with distilled water and further dried under laminar airflow on autoclaved blotting paper . The surface sterilized and dried seeds were treated by soaking in the spore suspensions of T. viride, T. asperellum, B. bassiana and the extract of C-PC. The control seeds were left un-primed (control) or primed only with sterilized distilled water (hydroprimed). Therefore, the treatments involved were T1: Non primed seeds (Control), T2: Hydropriming, T3: Biopriming with C-PC, T4: Biopriming with T. viride, T5: Biopriming with T. asperellum, T6: Biopriming with B. bassiana. Further, all the seeds were placed in the moist chamber at 98% relative humidity and 25–28 °C and maintained for 24 h , after that, they were air dried. Each treatment was replicated 4 times, so, a total of 24 Petri dishes (6*4) were used, each containing 10 seeds. Primed and unprimed tomato seeds were germinated in 9-cm diameter Petri dishes. The dishes were covered with a layer of absorbent cotton and blotter papers and were incubated at 25 ± 1 °C with supplemental day/night lighting of 16/8 h. After 12 days of growth, seedlings were collected from each treatment for measuring germination parameters and the rest seedlings were frozen in liquid nitrogen then immediately grinded in the suitable solvent for each biochemical parameter.
Bioassay on comparative effects of biopriming with fungal inoculums and C-PC extract on the germination indices and seedling growth
Seeds were considered germinated on radicle visibility (the radicle length was longer than 2.0 mm). After 12 days of growth, seedlings were collected from each treatment for measuring different germination indices and germination parameters to indicate the influence of different biostimulants. Germination percentage (%) , germination index (GI) , seedling length vigor index (SLVI)  and seedling weight vigor index (SWVI)  are examples of the germination indices.
After that, seedlings were harvested, and readings were taken regarding seedling growth according to ISTA protocols . Shoot height and radical length was measured in 5 normal seedlings randomly obtained. The seedling fresh weight (FW) and dry weight (DW) were recorded after oven drying at 60 °C for 48 h.
Bioassay on comparative effects of biopriming with fungal inoculums and C-PC extract on tomato seedlings primary metabolites contents
The total protein content
The fresh seedlings of known weight (1 g) were ground in a mortar and pestle using 50 mM phosphate buffer (pH 7). The resultant homogeneous solution was centrifuged at 8000 rpm for 15 min at 4 °C. Supernatant constituting the crude extract of amylase and protease was collected and aliquots were used for protein content and hydrolytic enzymes activity estimation. The protein content of tomato seedlings from each treatment was calculated  with some modifications. The mixture was again subjected to shaking for 10 mins after adding alkaline copper sulfate reagent and Folin’s reagent. The whole mixture was placed in an incubator for 30 min. The absorbance of each sample was recorded at 700 nm against blank. The concentration of total soluble proteins was determined with the reference curve of bovine serum albumin as a standard.
The total carbohydrates
A known tomato seedlings dry weight of all treatments were separately hydrolyzed in boiling water for 3 h with 10 mL 2.5 N HCl and then cooled. It was further neutralized with sodium carbonate and then centrifuged at 5000 rpm for 15 min and the supernatant (0.1 mL) was used for total carbohydrates estimation by phenol sulphuric acid method . The 2.5 mL of H2SO4 was added to the reaction mixtures and subjected to vigorous stirring followed by recording the absorbance at 490 nm. The amount of carbohydrates (mg/g DW) was calculated using the glucose standard curve.
Total free amino acids (TFAA)
A known seedlings fresh weight of tomato were extracted in 5 mL of 80% ethanol and centrifuged at 6000 rpm for 30 min to measure their TFAA contents by Yemm et al. . The test extract was taken and TFAA was estimated using ninhydrin reagents containing 1% ninhydrin in 0.5 M citrate buffer, pH 5.5, glycerol (87%) and 0.5 M citrate buffer pH 5.5 in the ratio of 5:12:2. After vigorous shaking contents were heated in a boiling water bath for 10 mins and after cooling, absorbance was measured at 570 nm with ethanol serving as blank in place of test extract. Absorbance readings were converted to mg amino acid g− 1 fresh weight of seedling using a glycine standard curve.
Bioassay on comparative effects of biopriming with fungal inoculums and C-PC extract on tomato seedlings secondary metabolites contents
The total phenolic content
The total phenolic content of the tomato seedlings was assessed from the seedling extract  after 95% ethanol extraction. According to the protocol, around 200 μL of the prepared extract was poured into a test tube with 1.4 mL of distilled water and 0.1 mL of 50% Folin-Ciocalteu phenol reagent. The sample was left for 3 mins and then sodium carbonate (0.4%) was added. The resultant mixture was kept for 2 h and then subjected to a gentle vortex. The absorbance was recorded at 650 nm. The gallic acid was used as a standard against which the total phenolic content was measured and expressed as mg/g FW of gallic acid equivalent (GAE).
The polyphenols (flavonoid and shikimic acid) content
The total flavonoid content of the tomato seedlings from different treatments was measured by following the AlCl3 colorimetric assay described by Zou et al.  using quercetin standard curve and was expressed as μg/g FW of quercetin equivalent (QE). Seedlings were taken and cut into very small pieces; 95% ethanol was added to make a fine paste. This mixture turned into a suspension and was subjected to centrifugation for 10 mins. The supernatant (200 μL) was collected and poured into a volumetric flask and around 5 mL of distilled water was added followed by the addition of 0.7 mL of 5% NaNO3 and 0.6 mL of 10% AlCl3. The resultant solution was put to rest for 5 mins. The solution was again left for 1 min after adding 3 mL of 1 M NaOH and 2.5 mL of distilled water and mixed thoroughly. The absorbance was recorded at 510 nm using a spectrophotometer versus a blank.
A known seedling fresh weight of tomato seedlings was ground in 2 mL 0.25 M HCl and then centrifuged for 30 min for determination of shikimic acid concentration according to Zelaya et al.  using the shikimic acid standard curve. The supernatant (50 μL) reacted with 0.5 mL of a 1% periodic acid and incubated at room temperature for 3 h. After incubation, 0.5 mL 1 M NaOH and 0.3 mL 0.1 M glycine were added. The absorbance was measured at 380 nm.
Determination of hydrolytic enzymes (Amylase and protease)
The supernatant obtained from homogenizing 1 g fresh seedlings with 50 mM phosphate buffer (pH 7) was used to estimate the hydrolytic enzymes activity. The amount of starch hydrolyzed by the action of amylases was measured according to Johnson . Protease activity was measured in an azocasein assay . Specific enzyme activity was expressed as change in optical density min− 1.
Statistical analysis, correlation analysis and figure preparation
The experimental design used in this study was carried out in a completely randomized design of 6 seedlings per treatment and each treatment was repeated in four sets. The obtained experimental data were processed by the mathematical and statistical methods using the SPSS software (version 15) statistical package. Descriptive statistics were used to process the obtained data which were expressed as mean ± Standard Error (SE). Comparison of mean values of all primed and non-primed samples were done using a One Way ANOVA test and Duncan’s test at p < 0.05. Pearson’s correlation coefficients (r) were carried out to understand the relationship between growth indices and different biochemical parameters using SPSS. Figures were assembled using OriginPro 8.5 for data analysis and graphing software.
Availability of data and materials
All data generated or analyzed during this study are included in this published article.
Gallic acid equivalent
Seedling length vigor index
Seedling weight vigor index
Total free amino acids
Hayat S, Ahmad H, Nasir M, Khan MN, Ali M, Hayat K, et al. Some physiological and biochemical mechanisms during seed-to-seedling transition in tomato as influenced by garlic allelochemicals. Antioxidants. 2020;9:235.
Vermeir I, Weijters B, De Houwer J, Geuens M, Slabbinck H, Spruyt A, et al. Environmentally sustainable food consumption: a review and research agenda from a goal-directed perspective. Front Psychol. 2020;11:1603. https://doi.org/10.3389/fpsyg.2020.01603.
Hayat S, Ahmad H, Ali M, Hayat K, Khan M, Cheng Z. Aqueous garlic extract as a plant biostimulant enhances physiology, improves crop quality and metabolite abundance, and primes the defense responses of receiver plants. Appl Sci. 2018;8:1505.
Wilson HT, Amirkhani M, Taylor AG. Evaluation of gelatin as a biostimulant seed treatment to improve plant performance. Front Plant Sci. 2018;9:1006.
Metwally RA, Soliman SA, Abdel Latef AA, Abdelhameed RE. The individual and interactive role of arbuscular mycorrhizal fungi and Trichoderma viride on growth, protein content, amino acids fractionation, and phosphatases enzyme activities of onion plants amended with fish waste. Ecotoxicol Environ Saf. 2021;214:112072.
Aamir M, Kashyap SP, Zehra A, Dubey MK, Singh VK, Ansari WA, Upadhyay RS, Singh S. Trichoderma erinaceum Biopriming Modulates the WRKYs Defense Programming in Tomato Against the Fusarium oxysporum f. sp. lycopersici (Fol) Challenged Condition. Front. Plant Sci. 2019;10:911. https://doi.org/10.3389/fpls.2019.00911.
Rouphael Y, Colla G. Editorial: biostimulants in agriculture. Front Plant Sci. 2020;11:40.
Feldmann F, Jehle J, Bradáčová K. et al. Biostimulants, soil improvers, bioprotectants: promoters of bio-intensification in plant production. J Plant Dis Prot. 2022. https://doi.org/10.1007/s41348-022-00567-x.
Muñoz-Fambuena N, Mesejo C, Carmen González-Mas M, Primo-Millo E, Agustí M, Iglesias DJ. Fruit regulates seasonal expression of flowering genes in alternate-bearing “Moncada” mandarin. Ann Bot. 2011;108:511–9.
Malo I, Bastiani MDe, Arevalo P, Bernacchia G. Natural extracts from pepper, wild rue and clove can activate defenses against pathogens in tomato plants. Eur J Plant Patol. 2017;149:89–101.
Calvo P, Nelson L, Kloepper JW. Agricultural uses of plant biostimulants. Plant Soil. 2014;383:3–41. 6.
Gioria M, Pyšek P. Early bird catches the worm: germination as a critical step in plant invasion. Biol Invasions. 2017;19:1055–80.
Jensen B, Knudsen IM, Madsen M, Jensen DF. Bio-priming of infected carrot seed with an antagonist, Clonostachys rosea, selected for control of seed borne Alternaria spp. Phytopathology. 2004;94:551–60.
Rocha I, Ma Y, Souza-Alonso P, Vosátka M, Freitas H, Oliveira RS. Seed coating: a tool for delivering beneficial microbes to agricultural crops. Front Plant Sci. 2019;10:1357.
Pill WG, Collins CM, Goldberger B, Gregory N. Responses of non-primed or primed seeds of ‘Marketmore 76’cucumber (Cucumis sativus L.) slurry coated with Trichoderma species to planting in growth media infested with Pythium aphanidermatum. Sci Hortic. 2009;121:54–62.
Singh V, Upadhyay RS, Sarma BK, Singh HB. Seed bio-priming with Trichoderma asperellum effectively modulate plant growth promotion in pea. Int J Agric Environ Biotechnol. 2016;9:361–5.
Wright B, Rowse H, Whipps JM. Microbial population dynamics on seeds during drum and steeping priming. Plant Soil. 2003;255(2):631–40. https://doi.org/10.1023/A:1026055112679.
Battacharyya D, Babgohari MZ, Rathor P, Prithiviraj B. Seaweed extracts as biostimulants in horticulture. Sci Hortic. 2015;196:39–48.
Zhang S, Gan Y, Xu B. Application of plant-growth-promoting Fungi Trichoderma longibrachiatum T6 enhances tolerance of wheat to salt stress through improvement of Antioxidative defense system and gene expression. Front Plant Sci. 2016;7:1405.
Osman MEH, Abo-Shady AM, El-Nagar MMF. Cyanobacterial Arthrospira (Spirulina platensis) as safener against harmful effects of fusilade herbicide on faba bean plant. Rend Fis Acc Lincei. 2016;27:455–62.
Wuang SC, Khin MC, Chua PQD, Luo YD. Use of Spirulina biomass produced from treatment of aquaculture wastewater as agricultural fertilizers. Algal Res. 2016;15:59–64.
Nawrocka D, Kornicka K, Śmieszek A, Marycz K. Spirulina platensis improves mitochondrial function impaired by elevated oxidative stress in adipose-derived mesenchymal stromal cells (ASCs) and intestinal epithelial cells (IECs), and enhances insulin sensitivity in equine metabolic syndrome (EMS) horses. Mar Drugs. 2017;15:237.
Koníčková R, Vaníková K, Vánová J, Muchová L, Subhanová I, Zadinová M, et al. Anti-cancer effects of blue-green alga Spirulina platensis, a natural source of bilirubin-like tetrapyrrolic compounds. Ann Hepatol. 2014;13(2):273–83.
Makhaye G, Aremu AO, Gerrano AS, Tesfay S, Du Plooy CP, Amoo SO. Biopriming with seaweed extract and microbial-based commercial biostimulants influences seed germination of five Abelmoschus esculentus genotypes. Plants. 2021;10:1327.
Martínez-Medina A, Fernandez I, Lok GB, Pozo MJ, Pieterse CM, Van Wees SC. Shifting from priming of salicylic acid-to jasmonic acid-regulated defences by Trichoderma protects tomato against the root knot nematode Meloidogyne incognita. New Phytol. 2017;213:1363–77. https://doi.org/10.1111/nph.14251.
Metwally RA. Arbuscular mycorrhizal fungi and Trichoderma viride cooperative effect on biochemical, mineral content, and protein pattern of onion plants. J Basic Microbiol. 2020;60(8):712-21. https://doi.org/10.1002/jobm.202000087.
Russo ML, Scorsetti AC, Vianna MF, Cabello M, Ferreri N, Pelizza S. Endophytic effects of Beauveria bassiana on corn (Zea mays) and its herbivore, Rachiplusia nu (Lepidoptera: Noctuidae). Insects. 2019;10(4):110.
Ranjan A, Ichihashi Y, Sinha NR. The tomato genome: implications for plant breeding, genomics and evolution. Genome Biol. 2012;13:167.
Jaiman RK, Acharya SK, Pathan NP, Deshmukh AJ. In vitro effect of seed bio-priming techniques on seed germination and seedling vigour of few vegetable crops. J Appl Nat Sci. 2020;12(4):702–9.
Eriksen NT. Production of phycocyanin—a pigment with applications in biology, biotechnology, foods and medicine. Appl Microbiol Biotechnol. 2008;80:1–14.
Kumar D, Wattal Dhar D, Pabbi S, Kumar N, Walia S. Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540). Indian J Plant Physiol. 2014;19(2):184–8.
Schipper K, Fortunati F, Oostlander PC, Al Muraikhi M, Al Jabri HMSJ, Wijffels RH, et al. Production of phycocyanin by Leptolyngbya sp. in desert environments. Algal. Research. 2020;47:101875.
Moovendhan M, Prabakaran G, Sampathkumar P, Kavisri M. Extraction and characterization of phycocyanin from Spirulina platensis and evaluation of its anticancer, antidiabetic and antiinflammatory effect. Int J Biol Macromol. 2020. https://doi.org/10.1016/j.ijbiomac.2020.03.009.
de Morais MG, da Fontoura PD, Moreira JB, Duarte JH, Costa JAV. (): Phycocyanin from microalgae: properties, extraction and purification, with some recent applications. Ind Biotechnol. 2018;14:30–7.
Safaei M, Maleki H, Soleimanpour H. Development of a novel method for the purification of C-phycocyanin pigment from a local cyanobacterial strain Limnothrix sp. NS01 and evaluation of its anticancer properties. Sci Rep. 2019;9:9474.
Seo YC, Choi WS, Park JH, Park JO, Jung KH, Lee HY. Stable isolation of Phycocyanin from Spirulina platensis associated with high-pressure extraction. Int J Mol Sci. 2013;14(1):1778–87. https://doi.org/10.3390/ijms14011778.
Gokel GW. Dean’s handbook of organic chemistry. New York: McGraw-Hill; 2004.
Al-Malki AL. In vitro cytotoxicity and pro-apoptotic activity of phycocyanin nanoparticles from Ulva lactuca (Chlorophyta) algae. Saudi J Biol Sci. 2020;27(3):894–8.
Wiegand G, Parbel A, Seifert MHJ, Holak A, Reuter W. Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides. Eur J Biochem. 2002;269:5046–55.
Mangoni O, Imperatore C, Tomas CR, Costantino V, Saggiomo V, Mangoni A. The new carotenoid pigment moraxanthin is associated with toxic microalgae. Mar Drugs. 2011;9:242–55.
Entesari M, Sharifzadeh F, Ahmadzadeh M, Farhangfar M. Seed biopriming with Trichoderma species and Pseudomonas fluorescent on growth parameters, enzymes activity and nutritional status of soybean. Int J Agron Plant Prod. 2013;4:610–9.
Sánchez-Rodríguez AR, Raya-Díaz S, María Zamarreño A, María García-Mina JM, del Campillo MC, Quesada-Moraga E. An endophytic Beauveria bassiana strain increases spike production in bread and durum wheat plants and effectively controls cotton leafworm (Spodoptera littoralis) larvae. Biol Control. 2018;116:90–102.
Metwally RA, Al-Amri SM. Individual and interactive role of Trichoderma viride and arbuscular mycorrhizal fungi on growth and pigment content of onion plants. Lett Appl Microbiol. 2020;70:79–86.
Macuphe N, Oguntibeju OO, Nchu F. Evaluating the endophytic activities of Beauveria bassiana on the physiology, growth, and antioxidant activities of extracts of lettuce (Lactuca sativa L.). Plants. 2021;10:1178.
Muñoz-Rojas M, Chilton A, Liyanage GS, Erickson TE, Merritt DJ, Neilan BA, et al. Effects of indigenous soil cyanobacteria on seed germination and seedling growth of arid species used in restoration. Plant Soil. 2018;429:91–100.
Chua M, Erickson TE, Merritt DJ, Chilton AM, Ooi MK, Muñoz-Rojas M. Bio-priming seeds with cyanobacteria: effects on native plant growth and soil properties. Restor Ecol. 2020;28:S168–76.
Haroun SA, Hussein MH. The promotive effect of algal biofertilizers on growth, protein pattern and some metabolic activities of Lupius termis plants grown in siliceous soil. Asian J Plant Sci. 2003;2:944–51.
Essa AMM, Ibrahim WM, Mahmud RM, Abo EKN. Potential impact of cyanobacterial exudates on seed germination and antioxidant enzymes of crop plant seedlings. Int J Curr Microbiol App Sci. 2015;4(6):1010–24.
Hashtroudi MS, Ghassempour A, Riahi H, Shariatmadari Z, Khanjir M. Endogenous auxins in plant growth-promoting cyanobacteria—Anabaena vaginicola and Nostoc calcicola. J Appl Phycol. 2013;25:379–86.
Ashraf MY, Naqvi MH. Effect of salinity on germination, seedling growth and mineral nutrition of mungbean (Vigna radiata L. Wileck). Pak J Agric Agric Eng Vet Sci. 1996;1:96–8.
Singh S, Singh UB, Malviya D, et al. Seed biopriming with microbial inoculant triggers local and systemic defense responses against Rhizoctonia solani causing banded leaf and sheath blight in maize (Zea mays L.). Int J Environ Res Public Health. 2020;17(4):1396.
Shoresh M, Harman GE. The molecular basis of shoot responses of maize seedlings to Trichoderma harzianum T22 inoculation of the root: a proteomic approach. Plant Physiol. 2008;147:2147–63.
Gomez-Vidal S, Salinas J, Tena M, Lopez-Llorca LV. Proteomic analysis of date palm (Phoenix dactylifera L.) responses to endophytic colonization by entomopathogenic fungi. Electrophoresis. 2009;30:2996–3005.
Idhan A, Nadir M, Kadir M. Paddy chlorophyll concentrations in drought stress condition and endophytic fungi application. IOP Conf Ser Earth Environ Sci. 2018;156:012040.
White JF, Torres MS. Is plant endophyte-mediated defensive mutualism the result of oxidative stress protection? Physiol Plant. 2010;138:440–6.
Mógor ÁF, Amatussi JDO, Mógor G, De Lara GB. Bioactivity of cyanobacterial biomass related to amino acids induces growth and metabolic changes on seedlings and yield gains of organic red beet. Am J Plant Sci. 2018;9:966–78.
Yakhin OI, Lubyanov AA, Yakhin IA, Brown PH. Biostimulants in plant science: a global perspective. Front Plant Sci. 2017;7(2049):1–32.
Gonçalves AL. Microalgae and Cyanobacteria in the improvement of agricultural practices: a review on their biofertilising, biostimulating and biopesticide roles. Appl Sci. 2021;11:871.
Khoulati A, Ouahhoud S, Mamri S, Meziane M, Choukri M, Asehraou A, et al. Valorization of Moroccan Crocus sativus L. by-products: foliar spraying of aqueous Tepal extract stimulates growth and confers antioxidant properties in eggplant seedling under greenhouse conditions. Biomed Res Int. 2020;2020:8812157.
Mohamed HI, Akladious SA. Changes in antioxidants potential, secondary metabolites and plant hormones induced by different fungicides treatment in cotton plants. Pest Biochem Physiol. 2017;142:117–22.
Metwally RA, Abdelhameed RE. Impact of Ridomil, Bavistin and Agrothoate on arbuscular mycorrhizal fungal colonization, biochemical changes and potassium content of cucumber plants. Ecotoxicology. 2019:28(5):487-98. https://doi.org/10.1007/s10646-019-02042-0.
Bennett RN, Wallsgrove RM. Secondary metabolites in plant defence mechanisms. New Phytol. 1994;127:617–33.
Pourtaghi E, Talaei-Hassanloui R, Nasibi F, Fotouhifar KB. Endophytic colonization of tomato by Beauveria bassiana for control of the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). Acta Biologica. 2020;27:149–60.
Singh A, Jain A, Sarma BK, Upadhyay RS, Singh HB. Rhizosphere microbes facilitate redox homeostasis in Cicer arietinum against biotic stress. Ann Appl Biol. 2013;163:33–46.
Zaprometov MN, Nikolaeva TN. Chloroplasts isolated from kidney bean leaves are vapable of phenolic compound biosynthesis. Russ. J Plant Physiol. 2003;50(5):623–6.
Kovaleva LV, Zakharova EV, Minkina YV. Auxin and flavonoids in the progame phase of fertilization in petunia. Russ J Plant Physiol. 2007;54(3):396–401.
Leather GR, Einhellig FA. Bioassay of naturally occurring allelochemicals for toxicity. J Chem Ecol. 1988;14:1821–8.
Moloinyane S, Nchu F. The effects of endophytic Beauveria bassiana inoculation on infestation level of Planococcus ficus, growth and volatile constituents of potted greenhouse grapevine (Vitis vinifera L.). Toxins. 2019;11(2):72.
Gasoni AB, Gurfinkel BS. Biocontrol of Rhizoctonia solani by the endophytic fungus Cladorrhinum foecundissimum in cotton plants. Australas Plant Pathol. 2009;38:389–91.
Goiris K, Muylaert K, Fraeye I, Foubert I, De Brabanter J, De Cooman L. Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. Environ Boil Fishes. 2012;24:1477–86.
Ma Z, Bykova NV, Igamberdiev AU. Cell signaling mechanisms and metabolic regulation of germination and dormancy in barley seeds. Crop J. 2017;5(6):459–77.
Robl D, Delabona PS, Mergel CM, et al. The capability of endophytic fungi for production of hemicellulases and related enzymes. BMC Biotechnol. 2013;13:94.
Caldwell BA, Jumpponen A, Trappe JM. Utilization of major detrital substrates by dark septate, root endophytes. Mycologia. 2000;92:230232.
Marlida Y, Saari N, Hassan Z, Radu S. Raw starch degrading enzyme from isolated strains of endophytic fungi. World J Microbiol Biotechnol. 2000;16:573–8.
Maria GL, Sridhar KR, Raviraj NS. Antimicrobial and enzyme activity of mangrove endophytic fungi of southwest coast of India. J Agric Technol. 2005;1:XX, 67–80.
Gholam A, Shahsavani S, Nezarat S. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. World Acad Sci Eng Technol. 2009;49:19–24.
Mabood F, Zhou X, Smith DL. Microbial signaling and plant growth promotion. Can J Plant Sci. 2014;94:1051–63.
Felse AP, Panda T. Production of microbial chitinases. Bioprocess Eng. 1999;23:127–34.
Osman MEH, El-Sheekh MM, El-Naggar AH, Gheda SF. Effect of two species of cyanobacteria as biofertilizers on some metabolic activities, growth, and yield of pea plant. Biol Fertil Soils. 2010;46:861–75.
Fatema F, Khan ZH, Khan ND, Mular SM. Determination of amylase activity from germinated Syzygium cumini seed (jamun). Int J Appl Res. 2017;3(1):573–5.
Ashraf MY, Azmi AR, Khan AH, Naqvi SSM. Alpha-amylase and protease activities of wheat seedling grown under water stress conditions. Pak J Sci Ind Res. 1995;38:430–4.
Zarrouk C. Contribution à l’étude d’une cyanophycée. Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de Arthrospira maxima. Geilter. PhD Thesis. France: Université de Paris; 1966.
Bennett A, Bogorad L. Comparative chromatic adaptation in a filamentous blue-green alga. J Cell Biol. 1973;58:419–35. https://doi.org/10.1083/jcb.58.2.419.
Lawrenz E, Fedewa EJ, Richardson TL. Extraction protocols for the quantification of phycobilins in aqueous phytoplankton extracts. J Appl Phycol. 2011;23:865–71.
Liao X, Zhang B, Wang X, Yan H, Zhang X. Purification of C-Phycocyanin from Spirulina platensis by single-step ion-exchange chromatography. Chromatographia. 2012;73(3):291–6.
Schanda P, Brutscher B. Very fast two-dimensional NMR spectroscopy for real time investigation of dynamic events in proteins on the time scale of seconds. J Am Chem Soc. 2005;127:8014–5.
Jain A, Singh S, Kumar Sarma B, Singh HB. Microbial consortium–mediated reprogramming of defence network in pea to enhance tolerance against Sclerotinia sclerotiorum. J Appl Microbiol. 2012;112:537–50.
Ghodrat V, Moradshahi A, Rousta MJ, Karampour A. Improving yield and yield components of rice (Oryza sativa L.) by indolebutyric acid (IBA), gibberellic acid (GA3) and salicylic acid (SA) pre-sowing seed treatments. Am-Eur J Agric Environ Sci. 2013;13(6):872–6.
Aloui H, Souguir M, Hannachi C. Determination of an optimal priming duration and concentration protocol for pepper seeds (Capsicum annuum L.). Acta Agric Slovenica. 2014;103(2):213–21.
Anupama N, Murali M, Sudisha J, Amruthesh KN. Crude oligosaccharides from Alternaria solani with Bacillus subtilis enhance defense activity and induce resistance against early blight disease of tomato. Asian J Sci Technol. 2014;5(7):412–6.
Heydariyan M, Basirani N, Sharifi-Rad M, Khmmari S, Poor SR. Effect of seed priming on germination and seedling growth of the caper (Capparis Spinosa) under drought stress. Int J Adv Biol Biomed Res. 2014;2(8):2381–9.
ISTA. Rules for testing seeds. Zurich: International Seed Testing Association (ISTA); 2019.
Lowry OH, Rosbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193:265–75.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Calorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350–6.
Yemm EW, Cocking EC, Ricketts RE. The determination of amino acids with ninhydrin. Analyst. 1955;80(948):209–14.
Jindal KK, Singh RN. Phenolic content in male and female Carica papaya: a possible physiological marker sex identification of vegetative seedlings. Physiol Plant. 1975;33(1):104–7.
Zou Y, Lu Y, Wei D. Antioxidant activity of flavonoid-rich extract of Hypericum perforatum L in vitro. J Agric Food Chem. 2004;52(16):5032–9.
Zelaya IA, Anderson JAH, Owen MDK, Landes RD. Evaluation of spectrophotometric and HPLC methods for shikimic acid determination in plants: models in glyphosate resistant and susceptible crops. J Agric Food Chem. 2011;59:2202–12.
Johnson R. Measuring amylase activity in cereal grains. Colby J Res Meth. 2007;9:11–3.
Mel SF, Fullner KJ, Wimer-Mackin S, Lencer WI, Mekala-nos JJ. Association of protease activity in Vibrio cholera vaccine strains with decrease in transcellular epithelial resistance of polarized T84 intestinal cells. Infect Immun. 2000;68:6487–92.
Experimental research and field studies on plants
All relevant institutional, national and international guidelines and legislation were complied or adhered to in the production of this study.
This work was supported by funds from Zagazig University, Egypt. Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).
Ethics approval and consent to participate
Consent for publication
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Metwally, R.A., Abdelhameed, R.E., Soliman, S.A. et al. Potential use of beneficial fungal microorganisms and C-phycocyanin extract for enhancing seed germination, seedling growth and biochemical traits of Solanum lycopersicum L.. BMC Microbiol 22, 108 (2022). https://doi.org/10.1186/s12866-022-02509-x
- Trichoderma sp.
- Flavonoid content
- Hydrolytic enzymes