- Research article
- Open Access
Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens
© Rychlik et al; licensee BioMed Central Ltd. 2009
- Received: 5 October 2009
- Accepted: 19 December 2009
- Published: 19 December 2009
Salmonella is a highly successful parasite of reptiles, birds and mammals. Its ability to infect and colonise such a broad range of hosts coincided with the introduction of new genetic determinants, among them 5 major pathogenicity islands (SPI1-5), into the Salmonella genome. However, only limited information is available on how each of these pathogenicity islands influences the ability of Salmonella to infect chickens. In this study, we therefore constructed Salmonella Enteritidis mutants with each SPI deleted separately, with single individual SPIs (i.e. with the remaining four deleted) and a mutant with all 5 SPIs deleted, and assessed their virulence in one-day-old chickens, together with the innate immune response of this host.
The mutant lacking all 5 major SPIs was still capable of colonising the caecum while colonisation of the liver and spleen was dependent on the presence of both SPI-1 and SPI-2. In contrast, the absence of SPI-3, SPI-4 or SPI-5 individually did not influence virulence of S. Enteritidis for chickens, but collectively they contributed to the colonisation of the spleen. Proinflammatory signalling and heterophil infiltration was dependent on intact SPI-1 only and not on other SPIs.
SPI-1 and SPI-2 are the two most important pathogenicity islands of Salmonella Enteritidis required for the colonisation of systemic sites in chickens.
- Nalidixic Acid
- Gene Cassette
- Pathogenicity Island
- Infected Chicken
- Resistance Gene Cassette
Salmonella diversified from a common ancestor with E. coli approx. 100 million years ago . This diversification was associated with the acquisition of genes which increased the virulence of Salmonella and enabled it to interact with its hosts and colonise the intestinal tract of animals in a different way than E. coli did. The genomic sequences of E. coli and S. enterica serovars Typhi and Typhimurium have been known since 1997 and 2001, respectively [2–4] and genes which are absent in E. coli and are necessary for the full virulence expression of Salmonella are therefore relatively well described. Most of them are clustered at specific parts of the Salmonella chromosome called pathogenicity islands. There are 5 major pathogenicity islands in the Salmonella enterica chromosome but only 4 of them, with SPI-2 absent, in the chromosome of Salmonella bongori, a second species belonging to the genus Salmonella .
The major pathogenicity islands include SPI-1, SPI-2, SPI-3, SPI-4 and SPI-5. The SPI-1 and SPI-2 genes code for proteins forming the type III secretion system (T3SS) which enable the transport of S. enterica proteins from the bacterial cell directly into the cytosol of eukaryotic cells. The SPI-1 encoded T3SS is required for the transport of S. enterica proteins across the cytoplasmic membrane of a host cell into its cytosol where they induce cytoskeletal rearrangements resulting in the uptake of S. enterica even by non-phagocytic cells . In addition, it has been reported that SPI-1 genes, independent of cell invasion, induce macrophage cytotoxicity . Interestingly, neither of these functions is required for the S. Typhimurium virulence for Balb/C mice since a mutant without the whole SPI-1 was as virulent as the control wild type strain . SPI-2 encoded T3SS is required for the transport of S. enterica proteins across the phagosomal membrane and increases S. enterica survival inside phagocytic cells [9, 10]. The function of genes localised on the remaining SPIs is less well characterised; SPI-3 genes are involved both in gut colonisation due to MisL-dependent fibronectin binding and intracellular survival due to high-affinity magnesium transport encoded by mgtABC [11, 12]. SPI-4 genes are required for the intestinal phase of disease by coding for non-fimbrial adhesin , and the genes localised in SPI-5 are co-regulated with either SPI-1 or SPI-2 genes and therefore code for effector proteins transported by either of these T3SS . However, the vast majority of this information has been obtained in a mouse model and S. Typhimurium and much less data are available for S. Enteritidis and pigs, cattle or poultry although these animal species, and poultry in particular, represent major reservoirs of Salmonella for the human population in Europe.
The roles of different SPI genes in the virulence S. enterica for chickens are less well understood. Similarly to mice, the importance of SPI-2 for Salmonella persistence in the internal organs has been described in chickens [15–17]. However, unlike the situation in mice, it seems that in chickens, SPI-1 genes are required for both the colonisation of the intestinal tract and the ability to reach and persist in internal organs such as the liver and spleen [17–19]. The importance of the other SPIs for Salmonella virulence in chickens is even less clear. To our knowledge, SPI-3 mutants have not been tested in chickens at all, SPI-4 mutants have been tested and shown to have no effect on chicken gut colonisation  and SPI-5 genes, although involved in the induction of the proinflammatory immune response in cattle, have been described as having no significant function in chickens [13, 20].
In this study we therefore compared virulence of isogenic mutants of S. enterica subsp. enterica serovar Enteritidis (S. Enteritidis) defective in 5 major pathogenicity islands for day-old chickens. To do this we deleted SPI-1 to SPI-5 from the S. Enteritidis chromosome and orally infected chickens with these mutants. Our data indicate that the colonisation of the liver and spleen by S. Enteritidis in chickens is dependent on SPI-1 and SPI-2 and that the remaining SPIs individually have no effect on S. Enteritidis virulence although collectively they had a low effect on spleen colonisation.
Infection of chickens - colonisation of the caecum, liver and spleen
S. Enteritidis 147 and its SPI mutants grouped according to their ability to colonise the liver and spleen of one-day-old chickens
Histology in chickens
Proinflammatory cytokine response
Previous experiments had shown that the early heterophil infiltration decreased with the loss of SPI-1. We therefore tested cytokine signalling in the caeca of chickens infected with the ΔSPI1, ΔSPI2 and ΔSPI1&2 mutants. For all the cytokines measured, an identical trend was observed - the highest induction was observed in chickens after infection with the wild type strain, followed by those infected with ΔSPI2, ΔSPI1 and ΔSPI1&2 mutants, respectively (data not shown). Except for IL-12β, the expression of the remaining cytokines after infection with the wild-type strain and the ΔSPI2 mutant significantly differed from the expression observed in non-infected control chickens while the differences between the non-infected chickens and those infected with the ΔSPI1 and ΔSPI1&2 mutant were always insignificant.
In this study we were interested in the role of five major pathogenicity islands in the virulence of S. Enteritidis for chickens. Rather unexpectedly, none of the pathogenicity islands was essential for colonisation of the intestinal tract despite the fact that other studies demonstrated that single gene SPI-1 mutants in chickens or SPI-4 mutants in cattle showed impaired intestinal colonisation and/or mucosa invasion [13, 18]. We cannot exclude the possibility that, if the infectious dose was changed or the duration of animal infection was extended for a longer period of time, we would observe a correlation between the persistence in the gut and the presence of a particular SPI. It is also possible that the differences between a single gene mutant and the whole SPI-1 mutant are biologically relevant because in mice a difference in the behaviour of the whole SPI-1 mutant and a hilA mutant was observed. This difference has been explained by the presence of the SPI-1 localised genes stimulating the host's immune response, the effect of which is suppressed in the presence of intact hilA . Finally, it is also possible that a low-level expression of SPI-1 encoded T3SS, which is likely to occur in the hilA mutant, might be still high enough to induce the interference with some of the eukaryotic host cell function(s) resulting in a ΔSPI1 mutant phenotype different from that of single gene mutants used in the above-mentioned other studies.
Although no influence of SPIs on gut colonisation was observed, SPI-1 and SPI-2 pathogenicity islands were both required for S. Enteritidis colonisation of the liver and spleen, similar to previous studies [9, 13, 18, 21]. Interestingly, the decrease in counts of the ΔSPI1 and ΔSPI2 mutants in the liver and spleen was numerically not as high as that observed for single gene SPI-2 mutants in mice . The importance of these two SPIs for S. Enteritidis colonisation of the liver and spleen of chickens was further supported by the behaviour of SPI1o and SPI2o mutants which, when compared with the ΔSPI1-5 mutant, had a significantly higher ability to colonise the spleen of infected chicken, and also by the ΔSPI1&2 mutant which did not differ in colonisation of liver and spleen from the ΔSPI1-5 mutant. Interestingly, the deletion of SPI-1 resulted in a significant difference from the wild type strain liver colonisation on day 5 but not on day 12 in agreement with the results of Desin et al.  suggesting that decreased liver colonisation by the ΔSPI1 mutant might be caused by its slower translocation through the gut epithelium. On the other hand, the ΔSPI2 mutant showed decreased liver colonisation both on day 5 and day 12 when compared with the wild-type strain, which is consistent with the role of SPI-2 encoded proteins in intra-macrophage survival . The importance of SPI-1 and SPI-2 was further confirmed by the virulence of SPI1o and SPI2o mutants because the presence of each of these pathogenicity islands individually increased the virulence of S. Enteritidis for chickens. Our observations on SPI-1 and SPI-2 as the most important SPIs are similar to those of Dieye et al. except for the fact that we could not confirm that SPI-1 would be more important than SPI-2 for Salmonella infection of chickens  although we did observe that SPI-1 was the most important for the induction of inflammation as supported by the cytokine inductions and the influx of heterophils. Interestingly, unlike the bovine and murine models [23, 24], we did not observe any correlation between the absence of SPI-2 and the induction of proinflammatory or any other cytokines in the avian caeca. Furthermore, we did not observe any effect of SPI-3, SPI-4 and SPI-5 deletions on the virulence of S. Enteritidis for chickens. This agrees with the observations of Morgan et al. who showed that SPI-4 genes were superfluous and SPI-3 genes and the pipB gene of SPI-5 played only a minor role in the colonisation of the chicken gut by S. Typhimurium . However since the SPI1&2o mutant showed reduced ability to colonise the spleen 4 days post infection when compared with the wild-type S. Enteritidis infection, this shows that SPI-3, SPI-4 and SPI-5 collectively influenced the virulence of S. Enteritidis for chickens although these 3 SPIs individually did not contribute to the ability of S. Enteritidis to colonise the spleen of infected chickens.
In this study we have shown that SPI-1 and SPI-2 pathogenicity islands are central to the virulence of S. Enteritidis for chickens. The presence of either of these two pathogenicity islands resulted in a significant increase in the liver and spleen colonisation by S. Enteritidis. The remaining three major pathogenicity islands (SPI-3, SPI-4 and SPI-5) influenced S. Enteritidis virulence for day-old chickens collectively but not individually.
Bacterial strains and culture conditions
S. Enteritidis strain 147 was used throughout the study . A clone spontaneously resistant to nalidixic acid was propagated in LB broth supplemented with ampicillin, chloramphenicol or kanamycin if necessary.
Construction and characterisation of SPI deletion mutants
List of primers used for the generation and verifications of SPI mutants in S. Enteritidis.
Sequence 5' - 3'
List of strains used in this study.
S. Enteritidis 147 Nal wild type
1, 2, 3, 4, 5
S. Enteritidis 147 Nal ΔSPI1
S. Enteritidis 147 Nal ΔSPI2
S. Enteritidis 147 Nal ΔSPI3
S. Enteritidis 147 Nal ΔSPI4
S. Enteritidis 147 Nal ΔSPI5
S. Enteritidis 147 Nal ΔSPI1-5
S. Enteritidis 147 Nal SPI1o
S. Enteritidis 147 Nal SPI2o
S. Enteritidis 147 Nal SPI3o
S. Enteritidis 147 Nal SPI4o
S. Enteritidis 147 Nal SPI5o
S. Enteritidis 147 Nal ΔSPI1&2
S. Enteritidis 147 Nal SPI1&2o
Infection of chickens
In the first experimental infection, day-old chickens (Ross breed, 10 birds/group) were infected orally with 5 × 107 CFU of either the wild-type strain or the SPI mutants. In the second infection, four groups, each of 10 chickens, were infected with the wild type strain, or ΔSPI1&2, SPI1&2o and SPI1-5 mutants. Counts of the strains in caeca, liver and spleen were determined in 5 birds on day 5 and in remaining 5 birds on day 12 of life i.e. 4 and 11 days post infection, respectively. The last experimental infection was focused on cytokine signaling and in this case, besides 3 non-infected control chickens, three additional chickens per group were infected with wild type strain, ΔSPI1, ΔSPI2, and ΔSPI1&2 mutants. In all euthanised birds, S. Enteritidis counts in the caeca, liver and spleen were determined after tissue homogenisation in peptone water and plating tenfold serial dilutions on XLD, BGA or Bromothymol-blue agars (Merck) supplemented with nalidixic acid. Samples negative after the direct plating were subjected to pre-enrichment in RV broth supplemented with nalidixic acid for qualitative S. Enteritidis determination. Counts of S. Enteritidis positive after the direct plating were logarithmically transformed. In the case of samples positive only after the pre-enrichment, these were assigned a value of 1 and the negative samples were assigned a value of 0. Samples from the caeca and liver were also fixed in 10% formaldehyde and subjected to haematoxylin and eosin staining. Each sample was blindly evaluated for general pathology with particular attention given to the infiltration of the caecal wall with heterophils by determining the average number of these cells per 20 independent microscopic fields. All the animal infections were performed according to the relevant national legislation and were approved and supervised by the Institutional Ethics Committee on Animal Experiments of Veterinary Medical Research Institute of Hungarian Academy of Sciences followed by the approval of the Veterinary and Food Control Station, Budapest, Hungary, and the Institutional Ethics Committee on Animal Experiments of Veterinary Research Institute Brno followed by the approval of the Animal Welfare Committee at the Ministry of Agriculture of the Czech Republic.
Real-time PCR cytokine quantification
List of primers used for the quantification of chicken cytokines after the infection with S. Enteritidis.
Sequence 5' - 3'
Product size (bp)
The threshold cycle values (Ct) were first normalised to reference GAPDH mRNA (ΔCt) and the normalised mRNA levels of genes of interest were calculated as 2(-ΔCt). The normalised mRNA levels of a particular cytokine were then used for the t-test comparison between the infected and non-infected birds. Finally, to display the fold induction after infection, 2(-ΔΔCt) values were calculated for each cytokine mRNA levels by subtracting the normalised average Ct of the gene of interest in the infected and non-infected chickens.
Statistics and reproducibility
ANOVA with Tuckey's post hoc test was used for the analysis of bacterial counts and heterophil infiltration in infected chickens. The cytokine responses of chickens infected with the particular mutants and those of the non-infected controls were compared by the t-test.
This work was supported by project MZE0002716201 of the Czech Ministry of Agriculture and project 524/09/0215 of the Czech Science Foundation. VMRI has been supported by EU-FP6 NoE MedVetNet. The excellent technical assistance of Michaela Dekanova is acknowledged. We also thank Dr. A. Szekely for his editorial assistance and Prof. Paul A. Barrow, University of Nottingham, UK, for English language corrections.
- Retchless AC, Lawrence JG: Temporal fragmentation of speciation in bacteria. Science. 2007, 317: 1093-1096. 10.1126/science.1144876.PubMedView ArticleGoogle Scholar
- Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, et al: The complete genome sequence of Escherichia coli K-12. Science. 1997, 277: 1453-1462. 10.1126/science.277.5331.1453.PubMedView ArticleGoogle Scholar
- McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, et al: Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001, 413: 852-856. 10.1038/35101614.PubMedView ArticleGoogle Scholar
- Parkhill J, Dougan G, James KD, Thomson NR, Pickard D, Wain J, Churcher C, Mungall KL, Bentley SD, Holden MT, et al: Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature. 2001, 413: 848-852. 10.1038/35101607.PubMedView ArticleGoogle Scholar
- Porwollik S, Wong RM, McClelland M: Evolutionary genomics of Salmonella: gene acquisitions revealed by microarray analysis. Proc Natl Acad Sci USA. 2002, 99: 8956-8961. 10.1073/pnas.122153699.PubMed CentralPubMedView ArticleGoogle Scholar
- Kaniga K, Trollinger D, Galan JE: Identification of two targets of the type III protein secretion system encoded by the inv and spa loci of Salmonella typhimurium that have homology to the Shigella IpaD and IpaA proteins. J Bacteriol. 1995, 177: 7078-7085.PubMed CentralPubMedGoogle Scholar
- Chen LM, Kaniga K, Galan JE: Salmonella spp. are cytotoxic for cultured macrophages. Mol Microbiol. 1996, 21: 1101-1115. 10.1046/j.1365-2958.1996.471410.x.PubMedView ArticleGoogle Scholar
- Murray RA, Lee CA: Invasion genes are not required for Salmonella enterica serovar typhimurium to breach the intestinal epithelium: evidence that salmonella pathogenicity island 1 has alternative functions during infection. Infect Immun. 2000, 68: 5050-5055. 10.1128/IAI.68.9.5050-5055.2000.PubMed CentralPubMedView ArticleGoogle Scholar
- Cirillo DM, Valdivia RH, Monack DM, Falkow S: Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival. Mol Microbiol. 1998, 30: 175-188. 10.1046/j.1365-2958.1998.01048.x.PubMedView ArticleGoogle Scholar
- Hensel M, Shea JE, Waterman SR, Mundy R, Nikolaus T, Banks G, Vazquez-Torres A, Gleeson C, Fang FC, Holden DW: Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages. Mol Microbiol. 1998, 30: 163-174. 10.1046/j.1365-2958.1998.01047.x.PubMedView ArticleGoogle Scholar
- Smith RL, Kaczmarek MT, Kucharski LM, Maguire ME: Magnesium transport in Salmonella typhimurium: regulation of mgtA and mgtCB during invasion of epithelial and macrophage cells. Microbiology. 1998, 144: 1835-1843. 10.1099/00221287-144-7-1835.PubMedView ArticleGoogle Scholar
- Dorsey CW, Laarakker MC, Humphries AD, Weening EH, Baumler AJ: Salmonella enterica serotype Typhimurium MisL is an intestinal colonization factor that binds fibronectin. Mol Microbiol. 2005, 57: 196-211. 10.1111/j.1365-2958.2005.04666.x.PubMedView ArticleGoogle Scholar
- Morgan E, Campbell JD, Rowe SC, Bispham J, Stevens MP, Bowen AJ, Barrow PA, Maskell DJ, Wallis TS: Identification of host-specific colonization factors of Salmonella enterica serovar Typhimurium. Mol Microbiol. 2004, 54: 994-1010. 10.1111/j.1365-2958.2004.04323.x.PubMedView ArticleGoogle Scholar
- Knodler LA, Celli J, Hardt WD, Vallance BA, Yip C, Finlay BB: Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems. Mol Microbiol. 2002, 43: 1089-1103. 10.1046/j.1365-2958.2002.02820.x.PubMedView ArticleGoogle Scholar
- Jones MA, Hulme SD, Barrow PA, Wigley P: The Salmonella pathogenicity island 1 and Salmonella pathogenicity island 2 type III secretion systems play a major role in pathogenesis of systemic disease and gastrointestinal tract colonization of Salmonella enterica serovar Typhimurium in the chicken. Avian Pathol. 2007, 36: 199-203. 10.1080/03079450701264118.PubMedView ArticleGoogle Scholar
- Bohez L, Gantois I, Ducatelle R, Pasmans F, Dewulf J, Haesebrouck F, Van Immerseel F: The Salmonella Pathogenicity Island 2 regulator ssrA promotes reproductive tract but not intestinal colonization in chickens. Vet Microbiol. 2008, 126: 216-224. 10.1016/j.vetmic.2007.06.025.PubMedView ArticleGoogle Scholar
- Dieye Y, Ameiss K, Mellata M, Curtiss R: The Salmonella Pathogenicity Island (SPI) 1 contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium. BMC Microbiol. 2009, 9: 3-10.1186/1471-2180-9-3.PubMed CentralPubMedView ArticleGoogle Scholar
- Bohez L, Ducatelle R, Pasmans F, Botteldoorn N, Haesebrouck F, Van Immerseel F: Salmonella enterica serovar Enteritidis colonization of the chicken caecum requires the HilA regulatory protein. Vet Microbiol. 2006, 116: 202-210. 10.1016/j.vetmic.2006.03.007.PubMedView ArticleGoogle Scholar
- Desin TS, Lam PK, Koch B, Mickael C, Berberov E, Wisner AL, Townsend HG, Potter AA, Koster W: Salmonella enterica serovar enteritidis pathogenicity island 1 is not essential for but facilitates rapid systemic spread in chickens. Infect Immun. 2009, 77: 2866-2875. 10.1128/IAI.00039-09.PubMed CentralPubMedView ArticleGoogle Scholar
- Galyov EE, Wood MW, Rosqvist R, Mullan PB, Watson PR, Hedges S, Wallis TS: A secreted effector protein of Salmonella dublin is translocated into eukaryotic cells and mediates inflammation and fluid secretion in infected ileal mucosa. Mol Microbiol. 1997, 25: 903-912. 10.1111/j.1365-2958.1997.mmi525.x.PubMedView ArticleGoogle Scholar
- Shea JE, Hensel M, Gleeson C, Holden DW: Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium. Proc Natl Acad Sci USA. 1996, 93: 2593-2597. 10.1073/pnas.93.6.2593.PubMed CentralPubMedView ArticleGoogle Scholar
- Karasova D, Sebkova A, Vrbas V, Havlickova H, Sisak F, Rychlik I: Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential. Vaccine. 2009, 27: 5265-5270. 10.1016/j.vaccine.2009.06.060.PubMedView ArticleGoogle Scholar
- Hapfelmeier S, Stecher B, Barthel M, Kremer M, Muller AJ, Heikenwalder M, Stallmach T, Hensel M, Pfeffer K, Akira S, et al: The Salmonella pathogenicity island (SPI)-2 and SPI-1 type III secretion systems allow Salmonella serovar typhimurium to trigger colitis via MyD88-dependent and MyD88-independent mechanisms. J Immunol. 2005, 174: 1675-1685.PubMedView ArticleGoogle Scholar
- Coombes BK, Coburn BA, Potter AA, Gomis S, Mirakhur K, Li Y, Finlay BB: Analysis of the contribution of Salmonella pathogenicity islands 1 and 2 to enteric disease progression using a novel bovine ileal loop model and a murine model of infectious enterocolitis. Infect Immun. 2005, 73: 7161-7169. 10.1128/IAI.73.11.7161-7169.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Methner U, Barrow PA, Gregorova D, Rychlik I: Intestinal colonisation-inhibition and virulence of Salmonella phoP, rpoS and ompC deletion mutants in chickens. Vet Microbiol. 2004, 98: 37-43. 10.1016/j.vetmic.2003.10.019.PubMedView ArticleGoogle Scholar
- Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA. 2000, 97: 6640-6645. 10.1073/pnas.120163297.PubMed CentralPubMedView ArticleGoogle Scholar
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