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Table 2 List of primers used for the generation and verifications of SPI mutants in S. Enteritidis.

From: Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens

SPI deletion

Primer *

Sequence 5' - 3'

SPI1

avrA_44F

TTATCGTTTAGCATAACGGCATTGTTATCGAATCGCTCATAAAGGTGTAGGCTGGAGCTGCTTC

SPI1

avrA_44R

ATGATATTTTCGGTGCAGGAGCTATCATGTGGAGGGAAAAGTATCATATGAATATCCTCCTTAG

SPI1

avrA_FCTR

AGACTTATATTCAGCTATCC

SPI1

avrA_RCTR

ACATAACCCTGCTGTACCTG

SPI1

invH_44Fw

AATATGAAAAAATTTTATAGCTGTCTTCCTGT CTTTTTACTGATCTTCGGAATAGGAACTTCAT

SPI1

invH_R44

ATGAGTTGCTCTTCATCTTCTTTCGAACGCAT GTATTGTGGATGCATATGAATATCCTCCTTAG

SPI1

invH_FCTR

CAGGAGTTTTTTTTGCTAGC

SPI1

invH_RCTR

CATGGGCAGCAAGTAACGTC

SPI2

STM1379_44F

TCAATCGAGCAACTTTTTGCCTTCCAGGTCGATGGCGATGTTTTGTGTAGGCTGGAGCTGCTTC

SPI2

STM1379_44R

ATCATGAAAAAAGTCAAAAAATTGTCTCTTACCGATTTAGTGCTCATATGAATATCCTCCTTAG

SPI2

STM1379_FCTR

ACCATTCAAGAGACAATTGG

SPI2

STM1379_RCTR

GTCCTGTTCTGGTACTACGC

SPI2

ssaU_44Fw

ATGAGCGAGAAAACAGAACAGCCTACAGAAAAGAAATTACGTGAACTTCGGAATAGGAACTTCA

SPI2

ssaU_44R

TTATGGTGTTTCGGTAGAATGCGCATAATCTA TCTTCATCACCACATATGAATATCCTCCTTAG

SPI2

ssaU_FCTF

TATGGTATTAGCCGATCTGG

SPI2

ssaU_RCTR

ACCTTTATCGTCAAGCACTG

SPI3

STM3752_44F

TCAACGTATAGAGCCATCCGGATAAAGATACATGCCTCCCTCCAGTGTAGGCTGGAGCTGCTTC

SPI3

sugR_44R

TCTTCATGCGGCGGCTGTTCTCCTCGCTTGTC GAGCATCCAGGTCATATGAATATCCTCCTTAG

SPI3

STM3752_FCTR

GGATATCGTCTGCAAAGAAG

SPI3

sugR_RCTR

CTAGATGTTCACGGTAGCTA

SPI3

mgtC_44Fw

TTATTGACTATCAATGCTCCAGTGAATTGCGGTGATATTATCGTACTTCGGAATAGGAACTTCA

SPI3

mgtC_44R

CCAGAAAAAATGGAGGAACGTATGTTAATGTTTCCTTATATTTACATATGAATATCCTCCTTAG

SPI3

mgtC_FCTR

ATGAATCCCCAAAATTAAGG

SPI3

mgtC_RCTR

AATCATCTGGCAAGTTAACG

SPI4

STM4257_44F

ATGGAAGACGAAAGTAATCCGTGGCCTAGTTTTGTTGATACATTGTGTAGGCTGGAGCTGCTTC

SPI4

STM4257_44R

TCACTCTGACACCTTTTTATTAATAGTCGTGATAATAGCTTTAC CATATGAATATCCTCCTTAG

SPI4

STM4257_FCTR

TAAAGCGTATTGGTAGCAGG

SPI4

STM4257_RCTR

TAATGCACACAAAGAACCTG

SPI4

STM4262_44Fw

CGTATAGCCGATATTCCAATATTTATTATATT TCTCATTGTTATACTTCGGAATAGGAACTTCA

SPI4

STM4262_44R

TAAACTCATCTAAGTTATCAAAAATTTGCTTCTCGGTATTCTCACATATGAATATCCTCCTTAG

SPI4

STM4262_FCTR

CAGTCTATCACAGCAAGGCA

SPI4

STM4262_RCTR

TTATCCGGAGAACAATCACG

SPI5

SPI5_44F

ATATCGGGGAAAACAGGTGTATCTGCGGTATT TAATCTATATGTGTGTAGGCTGGAGCTGCTTC

SPI5

SPI5_44R

GAAGATAAAACGATGCAAAATGCGCAGACGCT CGCCCGTCGCCTCATATGAATATCCTCCTTAG

SPI5

SPI5_FCTR

ATATGCGTAACTCATCAGTC

SPI5

SPI5_RCTR

GCTGCAAACGCTGGTTATGC

  1. * Primers "...44..." were used for amplification with pKD3 or pKD4 plasmid templates. Sequence used for annealing with the plasmids is underlined. Primers "...CTR..." were used in control PCRs, forward always with c1 or k1, and reverse always with c2 or k2.
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BMC Microbiology

ISSN: 1471-2180

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