Table 4 List of primers used for the quantification of chicken cytokines after the infection with S. Enteritidis.
Primer | Sequence 5' - 3' | Product size (bp) | Reference |
|---|---|---|---|
IL-8For | ATGAACGGCAAGCTTGGAGCT | 94 | this study |
IL-8Rev | GCAGCTCATTCCCCATCTT | Â | Â |
TNFαFor | AATTTGCAGGCTGTTTCTGC | 112 | this study |
TNFαRev | TATGAAGGTGGTGCAGATGG |  |  |
IL-12βFor | TGGTCCACGCTTTGCAGAT | 140 | [25] |
IL-12βRev | AAGGTTAAGGCGTGGCTTCTTA |  |  |
IL-18For | ACGTGGCAGCTTTTGAAGAT | 88 | this study |
IL-18Rev | GCGGTGGTTTTGTAACAGTG | Â | Â |
iNOSFor | GAACAGCCAGCTCATCCGATA | 103 | [25] |
iNOSRev | CCCAAGCTCAATGCACAACTT | Â | Â |
IFNγFor | GCCGCACATCAAACACATATCT | 207 | [25] |
IFNγRev | TGAGACTGGCTCCTTTTCCTT |  |  |
GAPDHFor | GTCAGCAATGCATCGTGCA | 102 | [25] |
GAPDHRev | GGCATGGACAGTGGTCATAAGA | Â | Â |