- Research article
- Open Access
Structure-based discovery of inhibitors of the YycG histidine kinase: New chemical leads to combat Staphylococcus epidermidis infections
BMC Microbiologyvolume 6, Article number: 96 (2006)
Coagulase-negative Staphylococcus epidermidis has become a major frequent cause of infections in relation to the use of implanted medical devices. The pathogenicity of S. epidermidis has been attributed to its capacity to form biofilms on surfaces of medical devices, which greatly increases its resistance to many conventional antibiotics and often results in chronic infection. It has an urgent need to design novel antibiotics against staphylococci infections, especially those can kill cells embedded in biofilm.
In this report, a series of novel inhibitors of the histidine kinase (HK) YycG protein of S. epidermidis were discovered first using structure-based virtual screening (SBVS) from a small molecular lead-compound library, followed by experimental validation. Of the 76 candidates derived by SBVS targeting of the homolog model of the YycG HATPase_c domain of S. epidermidis, seven compounds displayed significant activity in inhibiting S. epidermidis growth. Furthermore, five of them displayed bactericidal effects on both planktonic and biofilm cells of S. epidermidis. Except for one, the compounds were found to bind to the YycG protein and to inhibit its auto-phosphorylation in vitro, indicating that they are potential inhibitors of the YycG/YycF two-component system (TCS), which is essential in S. epidermidis. Importantly, all these compounds did not affect the stability of mammalian cells nor hemolytic activities at the concentrations used in our study.
These novel inhibitors of YycG histidine kinase thus are of potential value as leads for developing new antibiotics against infecting staphylococci. The structure-based virtual screening (SBVS) technology can be widely used in screening potential inhibitors of other bacterial TCSs, since it is more rapid and efficacious than traditional screening technology.
In recent years, coagulase-negative strains of Staphylococcus epidermidis have become frequent causes of infections in connection with surgically implanted medical devices [1, 2]. In parallel, the appearance of multi-resistant and vancomycin-resistant S. epidermidis strains has increased quickly due to the increasing use of antibiotics in hospitals . The primary pathogenicity trait of S. epidermidis has been associated with its ability to form biofilms on surfaces of medical devices, limiting severely the efficacy of many conventional antibiotics, and biofilms may also protect the bacteria against attacks from the host defence system [4, 5]. It has also been observed that aminoglycoside antibiotics may trigger biofilm formation in some bacteria . There is therefore an urgent need to design novel antibiotics against staphylococcus infections, especially in relation to biofilm development. Recently, the complete genome sequences of two S. epidermidis strains, viz. the non-biofilm-forming strain ATCC12228 and the biofilm-forming strain RP62A, have been published [7, 8], bringing about new opportunities to discover potential antimicrobial targets using in silico genome analyses.
Two-component system (TCS) control proteins, harboring histidine kinase (HK) and response transcription regulator activities, have been uncovered in most bacteria. Recently, the TCSs have attracted attention due to their potential as novel antibacterial targets, especially those required for regulation of bacterial growth and virulence in pathogenic microorganisms [9, 10]. One TCS, YycG/YycF, highly conserved and specific to low G+C Gram-positive bacteria has been shown to be essential for Bacillus subtilis and Staphylococcus aureus survival [11, 12]. Inhibitors of the YycG HK, such as synthetic imidazole and zerumbone derivatives, or aranorosinol B, obtained by screening acetone extracts from 4000 microbes, have been documented to be effective antibacterial agents against B. subtilis [13, 14]. Identification of this limited number of YycG inhibitors required laborious biological and chemical experiments, and the side-effects of these compounds on mammalian cells remain unclear. Moreover, B. subtilis may not be an optimal model organism to investigate biofilm formation, a process of major importance for the virulence of staphylococci. This prompted us to demonstrate that S. epidermidis possesses a homologous YycG/YycF TCS, and to investigate whether it would be an appropriate target for the design of novel antibacterial agents. As a prerequisite we set up a rapid and convenient procedure for screening novel inhibitors of the YycG/YycF TCS, testing the possible effects of these inhibitors on both planktonic and sessile bacteria, while using the extreme sensitivity of mammalian cells as a control to put aside compounds that would display a non-specific effect on membranes.
Upon binding, many small molecules may affect the functions of proteins. Functional analysis has been the basis of a variety of experiments, in which synthetic or purified small molecules have been used to probe the molecular mechanisms underlying the biological processes in which target proteins are involved. This chemistry-based approach has been coined "chemical biology" . Combinatorial chemistry and in vivo or in vitro High Throughput Screening (HTS) constitute preferred approaches for discovering active compounds against particular protein targets . A complementary approach is to use computational methods to identify active compounds (binders or hits) targeting the three-dimensional (3D) structure of the substrate binding pocket of a protein. This in silico approach is called Structure-Based Virtual Screening (SBVS) [16–19].
In the present study, we first identified the homologous YycG/YycF TCS in the genomes of the S. epidermidis ATCC12228 and RP62A strains. Next, a 3D structural model of the conserved HATPase_c domain of S. epidermidis YycG HK was constructed by using the homologous modeling approach. Subsequently, the SBVS method was used to search for potential YycG inhibitors from the SPECS chemical lead-compound database. Of the 76 candidates selected from the database by SBVS, seven compounds were active in inhibiting growth of S. epidermidis on plates or in liquid media. Five of these compounds displayed bactericidal effects on both planktonic and biofilm cells of S. epidermidis. Except for one, the compounds bound to the YycG protein and inhibited its auto-phosphorylation in vitro. These compounds displayed low cytotoxicity on mammalian cells and were not hemolytic, indicating that they may be good leads to develop new antibiotics against staphylococci infection.
In silico identification of the YycG/YycF TCS in S.epidermidis
Based on sequence homology, we identified likely counterparts of the YycG/YycF TCS (GenBank accession number: AY864800/AY864801) in the genomes of S. epidermidis ATCC12228 and RP62A. The sequence of the YycG protein in S. epidermidis is highly homologous to those of S. aureus and B. subtilis (92% and 46% identity, respectively). The same is true for the YycF protein (97% and 76% identity, respectively). As YycG and YycF are "persistent" proteins in Firmicutes  we can be confident that their function is preserved in the clade. More importantly, attempts to inactivate the orthologous yycG/yycF genes identified in S. epidermidis (this work) also failed with homologous recombination technology, indicating that this TCS is required for bacterial growth of S. epidermidis (data not shown). Domain analysis indicates that the YycG protein of S. epidermidis contains one transmembrane segment, and several domains identified in other proteins: HAMP (a domain present in Histidine kinases, Adenylyl cyclases, Methyl-accepting proteins and Phosphatases), PAS (a domain initially found to be common to Period circadian protein, Ah receptor nuclear translocator protein, and Single-minded protein), HisKA, and HATPase_c, all common to bacterial HKs . Moreover, the YycF protein of S. epidermidis possesses a conserved REC domain containing a phosphoacceptor site that may be phosphorylated by YycG, and a Trans_reg_C domain, belonging to the OmpR-like winged helix-turn-helix DNA-binding domains (Figure 1A). Interestingly, multi-alignment of HATPase_c domain sequences with counterparts of Bacteria from the structural Protein Data Bank (PDB) showed that the sequences around the ATP binding site in most bacteria were similar, showing four conserved important motifs – the N box, G1 box, F box and G2 box . This demonstrated that the ATP binding domain of HKs is highly conserved in bacteria, suggesting that it may be used as a potential target for antibacterial agents screening . Comparison of the YycG HATPase_c domain of S. epidermidis with similar domains in the database showed that the most homologous sequence was the similar domain of the E. coli osmosensor EnvZ, a TCS molecule, with 30% sequence identity and 49% conservative replacements (Figure 1B). EnvZ was therefore used as a template for modeling the 3D structure of the YycG HATPase_c domain of S. epidermidis.
A 3D model of the YycG HATPase_c domain of S. epidermidis
To search for potential inhibitors of YycG HK by virtual screening, we constructed a 3D model for the YycG HATPase_c domain of S. epidermidis based on the NMR structure of the homologous domain of the E. coli osmosensor EnvZ (PDB entry 1BXD) . The resulting model 3D structure is shown in Figure 2. The final structure was checked and validated using several programs such as Prostat and Profile-3D . This model superposed well with the NMR structure of the homologous domain of EnvZ, the root-mean-square deviation (RMSD) for the Cα atoms being about 1.708 (Figure 2A). The HATPase_c domain of YycG is thus predicted to fold in a similar way to that in EnvZ, containing five stranded β-sheets and four α-helices, which form a two-layered α/β sandwich structure (Figure 2B). The surface shape and the general features of the HATPase_c domain of YycG were further investigated by using the MOLCAD module of Sybyl 6.8 . The ATP binding site consists of two different cavities connected by a gorge-like channel (Figure 3). This structural prediction was used for virtual drug screening, bearing in mind that only further experimental evidence would validate the model.
Discovery of potential inhibitors of the S. epidermidis YycG HK by Virtual Screening
The ATP-binding pocket formed by residues within a radius of 5 Å around the ATP site of the YycG HATPase_c model of S. epidermidis was used as the target site for high throughput virtual screening (HTVS). In a first step, 85,000 potential drug-like molecules, constituting an in-house database (named SPECS_1), were selected from the SPECS database using the drug-selection filter developed by Zheng et al. . The SPECS_1 database was searched for potential binding molecule structures using the program DOCK4.0 [28, 29] in a primary screening. The most optimal 10,000 structures were subsequently re-scored using the FlexX program  and CSCORE , a consensus scoring method that integrates five popular scoring functions. Two hundred molecules passed this highly selective filter. Finally, 100 molecules were manually selected from the latter sample as inhibitor candidates, according to their molecular diversity, their shape complementarity, and their potential for forming hydrogen bonds in the binding pocket of the YycG HATPase_c domain. Of those 100 candidates, 76 compound samples could be purchased from the SPECS Company for further experimental assays.
Antimicrobial activities of potential YycG inhibitors in vitro
Since the YycG/YycF TCS is essential for growth and survival in B. subtilis and S. aureus [11, 12], its conservation in sequence and in genome organization in S. epidermidis strongly suggests that it is essential in this organism as well. To test this possibility, we explored whether the potential YycG inhibitor candidates obtained by virtual screening could inhibit bacterial growth. At a concentration of 200 μM in liquid culture in a first screening procedure, seven out of the 76 candidates completely inhibited growth of S. epidermidis. The seven inhibitors belong to four different classes of chemical structures: three thiazolidinone analogs (compounds 2, 5, and 7), two benzamide analogs (compounds 1 and 3), one furan derivative (compound 4) and one derivative of pyrimidinone (compound 6), as shown in Figure 4.
Subsequently, the Minimal Inhibitory Concentration (MIC) values of these 7 compounds were determined, using the standard tube-dilution assay (Table 1). These 7 compounds were further shown to have similar MIC values when tested against 4 clinical isolates of S. epidermidis from different patients in the Zhongshan Hospital of Shanghai, China (data not shown). Compounds 1–6 showed similar MIC values (see Table 1) on the S. epidermidis non-biofilm-forming strain ATCC12228 and on the biofilm-forming strain RP62A, whereas compound 7 was only effective against the non-biofilm-forming strain ATCC12228. We also investigated their inhibitory effect against other Gram-positive pathogenic cocci which possess the homologous YycG/YycF TCS. This experiment showed that compounds 2 and 5 were effective against S. aureus, Streptococcus pyogenes and Streptococcus mutans, compound 1 and 3 inhibited the growth of S. aureus and S. pyogenes, and compound 4 was only active against S. pyogenes. In contrast, none of these compounds were active against Gram-negative bacteria, such as Escherichia coli or Pseudomonas aeruginosa at the concentration of 200 μM. This is consistent with the absence of a YycG/YycF TCS counterpart in these genomes and, taken together, this is a first observation suggesting that these compounds are not likely to act by trivial interaction with membrane structures. Subsequently, we investigated the bactericidal activity of these compounds against S. epidermidis using a standard Minimal Bactericidal Concentration (MBC) assay. Five compounds (1–5) had their MBC values (about 4 times MIC) below the threshold (200 μM), and no obvious differences were observed between the biofilm-forming strain and non-biofilm-forming strain of S. epidermidis (Table 1).
Killing biofilm cells of S. epidermidis by potential YycG inhibitors
Standard MIC/MBC assays measure anti-microbial activities on planktonic bacteria, whereas antibiotic resistance is reported to be enhanced up to 1000-fold in the same cells when they develop in biofilms . We therefore measured the bactericidal effect of compound (1–7) on sessile cells of S. epidermidis. Vancomycin, one of the antibiotics usually used in multidrug-resistant staphylococci associated infections , was as a comparison (MBC/MIC = 4 μg/ml/1 μg/ml in S. epidermidis RP62A strain). Compared with vancomycin, the potential YycG inhibitors (compounds 1–5) showed better killing efficiencies against 24-hour-old biofilm cells of S. epidermidis at their MBC values, respectively (Figure 5). The best chemical (compound 5) reduced the CFU of the biofilm cells more than 100-fold (relative to an untreated control). In contrast, vancomycin caused no increased killing of the biofilm cells even when the concentration was increased to 128 μg/ml, relative to that of its MBC value of 4 μg/ml (data not shown). In addition, recent data by using confocal laser scanning microscope (CLSM) also confirmed that these compounds exhibited much better killing effects than vancomycin on cells embedded in mature biofilms of S. epidermidis clinical isolates (data not shown.
Binding affinity of potential YycG inhibitors to the YycG' protein
The in vivo effects of the inhibitors extracted from the in silico screening procedure are consistent with YycG being their target. However, there is no straightforward extrapolation from in silico design to accurate identification of a target in vivo. In order to confirm the interaction of the potential YycG inhibitors with their putative target protein, recombinant His-tagged YycG' protein (the fragment of YycG protein used in the in silico approach, approximately 34 kDa as indicated in Methods) was overproduced by using the pET28a plasmid, and purified using the ProBond™ Purification System (see Additional File 1, the protein purity is above 95%). The binding affinities of the seven putative YycG inhibitors to the YycG' protein were determined by using Surface Plasmon Resonance (SPR) in vitro. Six compounds (1–5 and 7) displayed high binding affinities to the YycG' protein (Table 2), whereas compound 6 only bound very poorly to the YycG' protein. All the 6 positive compounds displayed a significant and dose-dependent binding pattern in the SPR response. Five compounds (1–5) showed characteristic square-wave binding curves, indicating that these compounds form unstable complexes with the YycG' protein in a process of fast association and fast dissociation (only compound 1 was shown in Figure 6A). Compound 7 displayed a slow association and slow dissociation reaction, forming a relatively stable complex with the YycG' protein (Figure 6B). The data were fitted to a steady-state affinity model for compounds 1–5 and a kinetic-state model for compound 7 using the Biacore 3000 software to evaluate the corresponding binding affinities (KD values), as shown in Table 2. The KD value of compound 6 can not be calculated because of its very poor binding affinity to the YycG' protein.
Inhibition of the YycG' protein ATPase activity in vitro
The common characteristic of HKs is ATP-dependent auto-phosphorylation, associated with the conserved HATPase_c domain. We measured the effects of our potential YycG inhibitors on the protein ATPase activity by using the Kinase-Glo™ Luminescent Kinase assay. Firstly, the putative kinase activity of YycG' protein was measured by quantifying the amount of ATP remained in solution after reaction. A direct relationship existed between the luminescence measured with the Kinase-Glo™ Reagent and the amount of ATP (see Additional File 2A), indicating the sensitivity of this assay is good. After adding purified YycG' protein into the reaction system (4 μg protein in each reaction system), the luminescence was decreased in all the groups containing different ATP concentrations, compared with parallel groups without YycG' treatment (see Additional File 2B), since the kinase can hydrolyze ATP for its auto-phosphorylation. Keeping the ATP concentration constant (50 μM), the luminescence was continuously decreased following the amounts of purified YycG' protein increased (see Additional File 2C). The results indicated that the purified YycG' protein possesses the ATPase activity in vitro. At a concentration of 50 μM, compounds 1–5 and 7 decreased the ATPase activity of 4 μg YycG' protein in the presence of 3 μM ATP by 52% to 86% (52%, 61%, 70%, 76%, 73% and 86%, respectively), indicating that the binding affinities of these compounds to YycG' correlate well with their inhibitory activities of auto-phosphorylation of YycG'. In contrast, compound 6, which binds poorly to the YycG' protein, showed almost no activity against YycG' ATPase activity (approximately 7% inhibition at 50 μM). The concentrations needed to decrease YycG' ATPase activity to 50% (IC50 values) by the 6 active compounds (1–5 and 7) were calculated by gradual dilution of these compounds with an invariable concentration of protein (4 μg) and ATP (3 μM), as shown in Table 2. The IC50 value of compound 6 was above 200 μM under the same reaction conditions. As a comparison, the fragment of another HK protein SrrB' containing homologous domains with YycG' in S. epidermidis  was also expressed and purified to observed the inhibitory effect on protein phosphotylation by these 6 potential YycG inhibitors (1–5 and 7) (Supple. Table 1). At the concentration of 50 μM, only compound 5 displays much lower inhibitory effect (20%) on SrrB' than it does (73%) on YycG', while the other 5 compounds have almost no inhibitory effects.
Cytotoxicity and hemolysis of the antimicrobial compounds in vitro
While several of the compounds we identified could be used as excellent drug leads, acting on targets that are absent from eukaryotic cells, they still may display toxicity by interfering with unexpected targets. As a step to rule out this possibility we analyzed the cytotoxicity of compounds 1–7 on a Vero cell line (Vero 76, African Green-monkey) by using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method (the Cell Proliferation Kit I, Roche, see Methods). This approach would also provide us a very sensitive assay to draw aside a trivial action of these products on membrane lipid bilayers. Remarkably, as shown in Table 2, most CC50 values were higher than the highest inhibitor concentration used (200 μM), except in the case of two inhibitors (compounds 2 and 3) which had CC50 values below 200 μM (CC50 is the concentration that induces a 50% cytotoxicity effect on Vero cells). Furthermore, at the concentration of their respective MIC values, all these compounds displayed very low (< 10%) or almost no cytotoxicity (data not shown). It has been reported that some TCS inhibitors induce hemolysis of mammalian erythrocytes, which has hindered the further development and application of these inhibitors . This prompted us to analyze the possible membrane damage caused by our potential YycG inhibitors by examining hemolysis of healthy human erythrocytes possibly induced by these compounds. At their MIC concentrations, compounds 1–7 displayed no apparent hemolysis (< 5% of the zero control). This compares well with the effect of conventional antibiotics such as tetracycline and ciprofloxacin (Table 2 and Figure 7). At their 4 times MIC concentrations, only compound 2 showed a little hemolysis (~6% of the zero control), and at the highest concentration (200 μM), the hemolytic activity of compound 2 was increased to ~16% of the zero control.
Interaction models of potential YycG inhibitors to the target protein
As potential YycG inhibitors, six compounds (1–5 and 7) selected by in silico screening bound with high affinity to the YycG' protein and inhibited its ATPase activity in vitro. To further investigate the sites of interaction between the compounds and the YycG protein, and to develop a strategy for designing novel inhibitors, models of the interaction of the compounds with the YycG protein were analyzed based on docking simulations.
The surface of the YycG HATPase_c domain shows two significant hydrophobic areas located in the ATP-binding pocket. One is placed in the inner small cavity and is composed of residues Phe498, Val501, Phe56 and Ile53; the other is positioned in the front of the outer larger cavity, consisting of residues Phe46, Thr483, Ile484, Phe485, Met493, and Leu598 (Figure 3). Most of these residues are conserved in bacterial HKs. The binding conformations of the inhibitors designed in the present study in the ATP-binding pocket of the YycG HATPase_c domain are shown in Figure 8. Although their structures are diverse, they adopt similar interactions with the conserved domain. According to the interaction models, a four-point pharmacophore can be figured out for describing the binding of the inhibitors (Figure 9): the middle part of each inhibitor has two hydrogen bond acceptors, hydrogen bonding to Asn503 and Lys542, which stabilize each one of the six inhibitor candidates in the binding pocket; the hydrophobic moieties of the inhibitors fit well into the two hydrophobic cavities. Noticeably, the binding model revealed that Asn503 is of prime importance for stabilizing the interactions between the inhibitors and the YycG protein. This is well in agreement with previous biological function studies which demonstrated that the conserved Asn503 in the N box was necessary for ligand binding in both classes I and II TCS [35, 36]. In contrast, Lys542 is a residue specific to YycG. Our binding models have provided new possibilities for studies of the biological function of Lys542 in ligand binding and catalysis of YycG (Figure 9). Besides those interactions, the hydrophobic area (mainly constructed by residues Val500, Ile536, Ile538, Pro539, Thr592 and Ile594) may bind hydrophobic parts of different compounds and stabilize these in the inner hollow of the pocket. Most of these residues are conserved in homologous HKs (data not shown).
The YycG/YycF TCS is conserved and specific for low G+C Gram-positive bacteria such as B. subtilis, S. aureus, Streptococcus pneumoniae and Listeria monocytogenes [11, 12, 37, 38]. This TCS has been shown to be essential in B. subtilis  and could not be inactivated by direct mutation in S. aureus , and which also occurs in S. epidermidis (this work). Moreover, several genes involved in cell-wall biosynthesis and metabolism, such as teichoic acid biosynthesis protein F , and cell wall synthesis protein YpfP  were predicted to be regulated by this TCS in S. epidermidis based on the presence of a potential YycF consensus DNA binding recognition sequence similar to the one described in B. subtilis and S. aureus [42, 43], although this predicted result needs to be further verified (Qin et al, unpublished data). We have therefore explored the possibility of using the YycG HK as a potential target in a screening for new antibiotics.
Compared with biological and chemical screening for new antibiotics, the advantage of SBVS technology is the rapid, economical and efficient throughput. Furthermore, lead-compound databases provide ample sources for screening, whereas chemical synthesis is time consuming and expensive. As a case in point, the use of lead-compound databases has already led to the discovery of the diarylquinoline lead-compound against Mycobacterium tuberculosis .
Screening 80,000 possible compounds in silico we finally retained 7, among which 6 were promising candidates as potential YycG HK inhibitors. These 6 compounds bound to the YycG protein in vitro and inhibited its ATPase activity, while they were also active antimicrobials against S. epidermidis. This does not prove, however, that YycG is the only target when compounds interact with bacteria. In fact, we have found a total of 16 putative TCSs in the genome of S. epidermidis, including YycG/YycF. Because they have many core features in common, we cannot exclude that some of the compounds may also bind to other HKs. This would in fact enhance the antibiotic effect of the compounds. To investigate this possibility, we expressed and purified the fragment of another HK of S. epidermidis – SrrB (designated as SrrB' containing similar domains with YycG' described in Methods), which is involved in the TCS SrrB/SrrA . We analyzed the effects of the 6 active compounds (1–5 and 7) on the auto-phosphorylation of this purified protein. We observed that only compound 5 (at 50 μM) inhibited the auto-phosphorylation of SrrB'. The inhibition was approximately 20% lower than that demonstrated here on YycG' (73%) under the same reaction conditions (see Additional File 3). This suggests that the compounds display a certain degree of specificity dependent on the characteristics of the HATPase_c domain structure in the different HKs.
One of the interesting and challenging goals of new searches for anti-microbial compounds is to identify potential drugs which are equally active against planktonic and sessile bacteria (biofilms). The YycG inhibitor compounds described here were found to have somewhat reduced bactericidal effect on mature biofilms of S. epidermidis. These compounds were, however, much more efficient against sessile bacteria than the commonly used staphylococcus antibiotic, vancomycin. In fact, vancomycin was almost without effect against biofilm cells (even at 128 μg/ml), as also reported by others . This was accounted for in S. aureus by reduced penetration of vancomycin and delay of the exposure of the bacteria in the biofilm deeper layers . However, it remains unknown whether the situation is the same with our compounds in S. epidermidis biofilms. Interestingly, seventeen compounds among the 76 candidates we retained for further studies were active in inhibiting biofilm formation without interfering with bacterial growth. Most of them did not bind to the YycG' protein nor inhibit its auto-phosphorylation in vitro, indicating that these compounds are not potential inhibitors of the YycG HK (data not shown). Their targets in S. epidermidis and the mechanisms of inhibiting biofilm formation are still under investigation.
In previous studies, some potential inhibitors of histidine kinase appeared to display trivial side effects, such as membrane disruption, excessive protein binding or limited bioavailability. This prevented their further development . In this study, the potential YycG inhibitors displayed low cytotoxicity and low hemolysis to mammalian cells at the effective concentrations we used in vitro. We are now in the process of finding out the more effective derivatives, using those as leads. In future work, an appropriate animal model also needs to be established to investigate the effect of these compounds in vivo.
In our study, these novel inhibitors of YycG histidine kinase are considered as promising lead-compounds for developing new reagents against staphylococci infections. Furthermore, the structure-based virtual screening (SBVS) technology can be widely used for discovery of potential bacterial TCSs inhibitors in both Gram-positive and Gram-negative species, even on other "drug target" proteins. And it is more rapid and efficacious than traditional screening technology.
Bacterial strains, media and reagents
Staphylococcus epidermidis ATCC12228 and RP62A strains were purchased from the American Type Culture Collection (ATCC, Manassas, USA). Staphylococcus aureus ATCC29213, Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 were kindly provided by Dr. Bijie Hu at Zhongshan Hospital (Shanghai, China). S. epidermidis strains se527, se886, se847, seG203, Streptococcus pyogenes strain 144, and Streptococcus mutans strain 128 were all clinical isolates from Zhongshan Hospital and Huashan Hospital (Shanghai, China). If not stated otherwise, S. epidermidis and S. aureus strains were grown at 37°C in tryptic soy broth (TSB, Oxoid) containing 0.25% glucose, E. coli and P. aeruginosa strains were grown at 37°C in Luria-Bertani (LB, Oxoid).
All compounds used as inhibitor candidates were purchased from the SPECS Company in the Netherlands. Stock solutions of the compounds were prepared in dimethyl sulfoxide (DMSO). All other chemicals were of reagent grade or ultra-pure quality and purchased from Sigma.
Domain analysis was performed based on the SMART database . The complete genome sequences of the two S. epidermidis strains, ATCC12228 (NC_004461) and RP62A (NC_002976) were accessed from the National Center for Biotechnology Information (NCBI) genome database . The homologous sequences with the YycG HATPase_c domain of S. epidermidis were searched from the Protein Data Bank (PDB) , using the BLASTp program . ClustalX was used to align the protein sequences .
3D structure modeling of the YycG HATPase_c domain
The sequence of S. epidermidis HK YycG was retrieved from GenBank (accession number AY864800). The Align123 module encoded in InsightII  was used in the pair-wise sequence alignment. Using the secondary structure information of EnvZ (PDB entry 1BXD) , the sequence alignment was adjusted manually to obtain a fine alignment for 3D structure construction. The 3D model of the YycG HATPase_c domain was generated by using the MODELLER program  encoded in InsightII. Finally, the whole structural models were optimized using the Discover_3 module of InsightII with CVFF force field. Several structural analysis softwares such as Prostat and Profile-3D  were used to check the structure quality. The Prostat module of InsightII was used to analyze the properties of bonds, angles, and torsions. The Profile-3D program was used to check the structure and sequence compatibility.
Structure-based virtual screening
Before docking the small molecules of interest on the model structure, we delineated the general features that the binding pocket should have. Major residues possibly composing the ATP-binding site of YycG HATPase_c domain were identified by the sequence alignment with the osmolarity sensor protein EnvZ of E. coli, and the ATP-binding pocket was probed on the optimized 3D model of YycG HATPase_c domain using the SiteID program encoded in Sybyl6.8 . The surface (electrostatic, hydrophobic and hydrogen bonding) properties of the binding pocket of the YycG HATPase_c domain were calculated using the MOLCAD program encoded in Sybyl6.8. The ATP-binding pocket of the YycG HATPase_c domain was used as a target for screening the SPECS database using the docking approach . The SPECS database contains the structural information of 280,000 small molecules. The SPECS Company supplies all the compound samples collected from different sources. As a first step, the SPECS database was submitted to our own filter of drug-ability , non-drug-able molecules were eliminated from the database, and finally 85,000 potential drug-able molecules were selected out for docking screening. The program DOCK 4.0 [28, 29] was used for primary screening. Residues within a radius of 5 Å around the ATP-binding pocket of the YycG HATPase_c domain were used for constructing the grids for the docking screening. During the docking calculations, Kollman-all-atom charges  were assigned to the protein, and Gasterger-Hückel charges [57, 58] were assigned to the small molecules. Conformational flexibility of the compounds from the database was implemented in the docking search. During DOCK simulation, the ligand-receptor binding energy was approximated by the sum of the van der Waals and electrostatic interaction energies. After an initial evaluation of orientation and scoring, a grid-based minimization was carried out for the ligand to locate the nearest local energy minimum within the receptor binding site. Position and conformation of each docked molecule were optimized using the single anchor search and torsion minimization method of DOCK 4.0. The 10,000 compounds with the highest score as obtained by DOCK search were selected for a second round docking using the FlexX program , and CSCORE  was used to re-score the compounds. The virtual screening was performed on a 392-processor Sunway-1 supercomputer at the Shanghai Supercomputer Center.
Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) assays
MIC assays for the antibacterial activities of the compounds were performed according to the broth micro-dilution (in tubes) method of the Clinical and Laboratory Standards Institute (CLSI) of America . The Minimal Bactericidal Concentration (MBC) was obtained by subculturing 100 μl from each negative (no visible bacterial growth) tube from the MIC assay, onto substance-free Mueller-Hinton agar plates. The plates were incubated at 37°C for 24 hours, and the MBC was defined as the lowest concentration of substance which produced subcultures growing no more than five colonies on each plate.
Killing biofilm cells of S. epidermidis by potential YycG inhibitors
An overnight culture of S. epidermidis strain RP62A was diluted 1:100 in TSB containing 0.25% glucose, then 1 ml bacterial suspension was inoculated into the wells of sterile 12-well polystyrene microtiter plates (Falcon) incubated at 37°C for 24 h. The plates with mature biofilm were washed gently four times with sterile PBS before adding fresh TSB containing the various compounds at their MBC values, and incubated at 37°C for 24 h. The plates were washed again four times with sterile PBS, then the biofilm cells were scraped from the plate and resuspended in 1 ml PBS, and violently vortexed to disintegrate clumps of cells. Next, the suspension was diluted gradually with sterile PBS and subcultured onto substance-free Mueller-Hinton agar plates, incubated at 37°C for 24 h, and the colonies were counted. The fresh medium containing vancomycin at the MBC value and substance-free fresh medium served as the controls. The experiment was repeated three times.
Cloning, expression and purification of the YycG' protein
The YycG' fragment containing the cytoplasmic signal domains (the HATPase_c and HisKA domain, see Figure 1A) of YycG (370aa to 610aa) was amplified by PCR (with the chromosomal DNA of S. epidermidis ATCC12228 as the template, and (5' GCGGATCCACAACAACAAGTCGAACGTGAAC 3') and (5' GCCTCGAGTTATTCATCCCAATCACCGTCT 3') as the primers. Subsequently, the fragment was digested with BamHI and XholI (TAKARA, Japan) and ligated into the corresponding sites of pET28a (Promega, Madison, USA) to obtain pETYycG'. The expressed YycG' protein was purified with the ProBond™ Purification System (Invitrogen, California, USA), according to the manufacturer's protocol.
Compound-YycG' protein binding assay
The in vitro binding affinities of the inhibitor compounds to the YycG' protein was determined using surface plasmon resonance (SPR) biosensor technology on the dual flow cell Biacore 3000 instrument (Biacore AB, Uppsala, Sweden) with a similar method as described in our previous study . Immobilization of the YycG' protein to the hydrophilic carboxymethylated dextran matrix of the sensor chip CM5 (Biacore) was carried out by the standard primary amine coupling reaction. The protein to be covalently bound to the matrix was diluted in 10 mM sodium acetate buffer (pH 4.2) to a final concentration of 0.3 mg/ml. Equilibration of the baseline was completed by a continuous flow of HBS-EP running buffer (10 mM HEPES, 150 mM NaCl, 3.4 mM EDTA and 0.005% (v/v) surfactant P20, pH 7.4) through the chip for 1–2 hours. Biacore data were collected at 25°C with HBS-EP as running buffer at a constant flow of 20 ml/min. Sensorgrams were processed by using automatic corrections for non-specific bulk refractive index effects. The equilibrium constants (KD) evaluating the protein-ligand binding affinity were determined by the steady state or the kinetic state affinity fitting model encoded in the Biacore analysis software.
Inhibition assay for the ATPase activity
The inhibitory activities of the compounds for the ATPase activity of the YycG' protein was measured using the Kinase-Glo™ Luminescent Kinase Assay (Promega, Madison, USA). Briefly, 4 μg YycG' protein was pre-incubated with a series of dilution of compounds in the reaction buffer (40 mM Tris [pH 7.5], 20 mM MgCl2, and 0.1 mg/ml BSA), at 25°C for 30 min. Then 3 μM ATP was added for another incubation of 30 min at 25°C, and Kinase-Glo™ Reagent was added to detect the rest amount of ATP, as recorded from luminescence measurements (RLU). In parallel, theYycG' protein with no addition of compounds was used as the control. The rate of inhibiting protein phosphorylation (Rp) by the compounds was calculated from equation 1:
IC50 (the concentration of inhibition of 50% YycG' protein autophosphorylation) was obtained by using the Origin v7.0 software (OriginLab, Northampton, USA).
Cytotoxicity and erythrocyte hemolysis assays
Cytotoxicity of the antibacterial compounds on cultured Vero cell was measured by using the Cell Proliferation Kit I (MTT) (Roche, Indianapolis, USA) according to the manufacturer's protocol. Addition of DMSO (1%) in the medium produced a slight cytotoxicity on Vero cell, which could easily be corrected for. Each assay was performed in quadruplicate and repeated three times. The results were converted to percentage of the control (cells only treated with 1% DMSO) and CC50 (concentrations that produce a 50% cytotoxicity effect on Vero cell) was calculated by the Origin v7.0 software (OriginLab, Northampton, USA). Hemolytic activities of the compounds were determined by using healthy human erythrocytes . The erythrocytes were washed three times in sterile saline and resuspended to 5% prior to the assay. Then a volume of 200 μl cell suspension containing MIC or 4×MIC concentrations of the compounds was added in quadruplicate to the wells of 96-well microtiter plates (Falcon). Cells without compound treatment and cells with 1% Triton-100 treatment were used as 0% and 100% hemolysis controls, respectively. The cell suspensions were incubated for 1 hour at 37°C and centrifuged at 1000×g for 10 min. Volumes of 100 μl supernatants were transferred to another sterile plate and hemoglobin release from the cells was determined at 570 nm. Addition of DMSO (1%) in the medium did not affect the integrity of erythrocyte membrane. The hemolysis assays were repeated twice.
Pharmacophore model building
The best conformations of the six inhibitor outputs from Dock4.0 were superimposed using the DISCO program  encoded in Sybyl6.8. Multi-conformations were generated for the Flex Searches using Multisearch and REJECT features. The REJECT feature removes duplicates to leave a set of unique low energy conformers. DISCO produces a number of possible pharmacophores. The hypotheses were grouped on the basis of the assignment of atoms to features. Four features containing two hydrogen donor and two hydrophobic centers were detected by DISCO.
structure-based virtual screening
high throughput screening
minimal inhibitory concentration
surface plasmon resonance.
Rupp ME, Archer GL: Coagulase-negative staphylococci: pathogens associated with medical progress. Clin Infect Dis. 1994, 19 (2): 231-243. quiz 244-235.
Huebner J, Goldmann DA: Coagulase-negative staphylococci: role as pathogens. Annu Rev Med. 1999, 50: 223-236. 10.1146/annurev.med.50.1.223.
Raad I, Alrahwan A, Rolston K: Staphylococcus epidermidis: emerging resistance and need for alternative agents. Clin Infect Dis. 1998, 26 (5): 1182-1187.
Donlan RM, Costerton JW: Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002, 15 (2): 167-193. 10.1128/CMR.15.2.167-193.2002.
Vandecasteele SJ, Peetermans WE, Merckx R, Van Eldere J: Expression of biofilm-associated genes in Staphylococcus epidermidis during in vitro and in vivo foreign body infections. J Infect Dis. 2003, 188 (5): 730-737. 10.1086/377452.
Hoffman LR, D'Argenio DA, MacCoss MJ, Zhang Z, Jones RA, Miller SI: Aminoglycoside antibiotics induce bacterial biofilm formation. Nature. 2005, 436 (7054): 1171-1175. 10.1038/nature03912.
Zhang YQ, Ren SX, Li HL, Wang YX, Fu G, Yang J, Qin ZQ, Miao YG, Wang WY, Chen RS, et al.: Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol Microbiol. 2003, 49 (6): 1577-1593. 10.1046/j.1365-2958.2003.03671.x.
Gill SR, Fouts DE, Archer GL, Mongodin EF, Deboy RT, Ravel J, Paulsen IT, Kolonay JF, Brinkac L, Beanan M, et al.: Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. J Bacteriol. 2005, 187 (7): 2426-2438. 10.1128/JB.187.7.2426-2438.2005.
Barrett JF, Hoch JA: Two-component signal transduction as a target for microbial anti-infective therapy. Antimicrob Agents Chemother. 1998, 42 (7): 1529-1536.
Stephenson K, Hoch JA: Virulence- and antibiotic resistance-associated two-component signal transduction systems of Gram-positive pathogenic bacteria as targets for antimicrobial therapy. Pharmacol Ther. 2002, 93 (2–3): 293-305. 10.1016/S0163-7258(02)00198-5.
Fabret C, Hoch JA: A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. J Bacteriol. 1998, 180 (23): 6375-6383.
Martin PK, Li T, Sun D, Biek DP, Schmid MB: Role in cell permeability of an essential two-component system in Staphylococcus aureus. J Bacteriol. 1999, 181 (12): 3666-3673.
Yamamoto K, Kitayama T, Minagawa S, Watanabe T, Sawada S, Okamoto T, Utsumi R: Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci Biotechnol Biochem. 2001, 65 (10): 2306-2310. 10.1271/bbb.65.2306.
Watanabe T, Hashimoto Y, Yamamoto K, Hirao K, Ishihama A, Hino M, Utsumi R: Isolation and characterization of inhibitors of the essential histidine kinase, YycG in Bacillus subtilis and Staphylococcus aureus. J Antibiot (Tokyo). 2003, 56 (12): 1045-1052.
Stockwell BR: Exploring biology with small organic molecules. Nature. 2004, 432 (7019): 846-854. 10.1038/nature03196.
Shen J, Xu X, Cheng F, Liu H, Luo X, Shen J, Chen K, Zhao W, Shen X, Jiang H: Virtual screening on natural products for discovering active compounds and target information. Curr Med Chem. 2003, 10 (21): 2327-2342. 10.2174/0929867033456729.
Jorgensen WL: The many roles of computation in drug discovery. Science. 2004, 303 (5665): 1813-1818. 10.1126/science.1096361.
Oprea TI, Matter H: Integrating virtual screening in lead discovery. Curr Opin Chem Biol. 2004, 8 (4): 349-358. 10.1016/j.cbpa.2004.06.008.
Abagyan R, Totrov M: High-throughput docking for lead generation. Curr Opin Chem Biol. 2001, 5 (4): 375-382. 10.1016/S1367-5931(00)00217-9.
Fang G, Rocha E, Danchin A: How essential are nonessential genes?. Mol Biol Evol. 2005, 22 (11): 2147-2156. 10.1093/molbev/msi211.
Galperin MY, Nikolskaya AN, Koonin EV: Novel domains of the prokaryotic two-component signal transduction systems. FEMS Microbiol Lett. 2001, 203 (1): 11-21. 10.1111/j.1574-6968.2001.tb10814.x.
Kim D, Forst S: Genomic analysis of the histidine kinase family in bacteria and archaea. Microbiology. 2001, 147 (Pt 5): 1197-1212.
Matsushita M, Janda KD: Histidine kinases as targets for new antimicrobial agents. Bioorg Med Chem. 2002, 10 (4): 855-867. 10.1016/S0968-0896(01)00355-8.
Tanaka T, Saha SK, Tomomori C, Ishima R, Liu D, Tong KI, Park H, Dutta R, Qin L, Swindells MB, et al.: NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. Nature. 1998, 396 (6706): 88-92. 10.1038/23968.
Bowie JU, Luthy R, Eisenberg D: A method to identify protein sequences that fold into a known three-dimensional structure. Science. 1991, 253 (5016): 164-170. 10.1126/science.1853201.
Sybyl. [molecular modeling package]. 2000, St Louis (MO): Tripos Associates, Version 6.8
Zheng S, Luo X, Chen G, Zhu W, Shen J, Chen K, Jiang H: A new rapid and effective chemistry space filter in recognizing a druglike database. J Chem Inf Model. 2005, 45 (4): 856-862. 10.1021/ci050031j.
Ewing TJ, Makino S, Skillman AG, Kuntz ID: DOCK 4.0: search strategies for automated molecular docking of flexible molecule databases. J Comput Aided Mol Des. 2001, 15 (5): 411-428. 10.1023/A:1011115820450.
Kuntz ID: Structure-based strategies for drug design and discovery. Science. 1992, 257 (5073): 1078-1082. 10.1126/science.257.5073.1078.
Rarey M, Kramer B, Lengauer T, Klebe G: A fast flexible docking method using an incremental construction algorithm. J Mol Biol. 1996, 261 (3): 470-489. 10.1006/jmbi.1996.0477.
Clark RD, Strizhev A, Leonard JM, Blake JF, Matthew JB: Consensus scoring for ligand/protein interactions. J Mol Graph Model. 2002, 20 (4): 281-295. 10.1016/S1093-3263(01)00125-5.
Ceri H, Olson ME, Stremick C, Read RR, Morck D, Buret A: The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol. 1999, 37 (6): 1771-1776.
Hiramatsu K: Vancomycin-resistant Staphylococcus aureus: a new model of antibiotic resistance. Lancet Infect Dis. 2001, 1 (3): 147-155. 10.1016/S1473-3099(01)00091-3.
Macielag MJ, Goldschmidt R: Inhibitors of bacterial two-component signalling systems. Expert Opin Investig Drugs. 2000, 9 (10): 2351-2369. 10.1517/135437184.108.40.2061.
Bilwes AM, Quezada CM, Croal LR, Crane BR, Simon MI: Nucleotide binding by the histidine kinase CheA. Nat Struct Biol. 2001, 8 (4): 353-360. 10.1038/86243.
Marina A, Mott C, Auyzenberg A, Hendrickson WA, Waldburger CD: Structural and mutational analysis of the PhoQ histidine kinase catalytic domain. Insight into the reaction mechanism. J Biol Chem. 2001, 276 (44): 41182-41190. 10.1074/jbc.M106080200.
Lange R, Wagner C, de Saizieu A, Flint N, Molnos J, Stieger M, Caspers P, Kamber M, Keck W, Amrein KE: Domain organization and molecular characterization of 13 two-component systems identified by genome sequencing of Streptococcus pneumoniae. Gene. 1999, 237 (1): 223-234. 10.1016/S0378-1119(99)00266-8.
Kallipolitis BH, Ingmer H: Listeria monocytogenes response regulators important for stress tolerance and pathogenesis. FEMS Microbiol Lett. 2001, 204 (1): 111-115. 10.1111/j.1574-6968.2001.tb10872.x.
Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, et al.: Essential Bacillus subtilis genes. Proc Natl Acad Sci USA. 2003, 100 (8): 4678-4683. 10.1073/pnas.0730515100.
Fischer W: Lipoteichoic acid and lipids in the membrane of Staphylococcus aureus. Med Microbiol Immunol (Berl). 1994, 183 (2): 61-76. 10.1007/BF00277157.
Kiriukhin MY, Debabov DV, Shinabarger DL, Neuhaus FC: Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase. J Bacteriol. 2001, 183 (11): 3506-3514. 10.1128/JB.183.11.3506-3514.2001.
Howell A, Dubrac S, Andersen KK, Noone D, Fert J, Msadek T, Devine K: Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach. Mol Microbiol. 2003, 49 (6): 1639-1655. 10.1046/j.1365-2958.2003.03661.x.
Dubrac S, Msadek T: Identification of genes controlled by the essential YycG/YycF two-component system of Staphylococcus aureus. J Bacteriol. 2004, 186 (4): 1175-1181. 10.1128/JB.186.4.1175-1181.2004.
Andries K, Verhasselt P, Guillemont J, Gohlmann HW, Neefs JM, Winkler H, Van Gestel J, Timmerman P, Zhu M, Lee E, et al.: A diarylquinoline drug active on the ATP synthase of Mycobacterium tuberculosis. Science. 2005, 307 (5707): 223-227. 10.1126/science.1106753.
Pragman AA, Yarwood JM, Tripp TJ, Schlievert PM: Characterization of virulence factor regulation by SrrAB, a two-component system in Staphylococcus aureus. J Bacteriol. 2004, 186 (8): 2430-2438. 10.1128/JB.186.8.2430-2438.2004.
Monzon M, Oteiza C, Leiva J, Lamata M, Amorena B: Biofilm testing of Staphylococcus epidermidis clinical isolates: low performance of vancomycin in relation to other antibiotics. Diagn Microbiol Infect Dis. 2002, 44 (4): 319-324. 10.1016/S0732-8893(02)00464-9.
Jefferson KK, Goldmann DA, Pier GB: Use of confocal microscopy to analyze the rate of vancomycin penetration through Staphylococcus aureus biofilms. Antimicrob Agents Chemother. 2005, 49 (6): 2467-2473. 10.1128/AAC.49.6.2467-2473.2005.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25 (24): 4876-4882. 10.1093/nar/25.24.4876.
InsightII. [molecular modeling package] Version. 2000, Calif (USA): Molecular Simulations Inc
Sali A, Blundell TL: Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol. 1993, 234 (3): 779-815. 10.1006/jmbi.1993.1626.
Shoichet BK, McGovern SL, Wei B, Irwin JJ: Lead discovery using molecular docking. Curr Opin Chem Biol. 2002, 6 (4): 439-446. 10.1016/S1367-5931(02)00339-3.
Cornell W: Quality communication supported by database. Nurs Qual Connect. 1995, 5 (1): 1, 8-
Gasteiger J, Marsili M: Iterative partial equalization of orbital electronegativity – a rapid access to atomic charges. Tetrahedron. 1980, 36: 3219-3228. 10.1016/0040-4020(80)80168-2.
Purcell WP, Singer JA: A brief review and table of semiempirical parameters used in the Hueckel molecular orbital method. J Chem Eng Data. 1967, 12: 235-246. 10.1021/je60033a020.
Clinical and Laboratory Standards Institute: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 7th ed. Approved standard M7-A7. 2006, Clinical and Laboratory Standards Institute, Villanova, Pa
Chen S, Chen L, Tan J, Chen J, Du L, Sun T, Shen J, Chen K, Jiang H, Shen X: Severe acute respiratory syndrome coronavirus 3C-like proteinase N terminus is indispensable for proteolytic activity but not for enzyme dimerization. Biochemical and thermodynamic investigation in conjunction with molecular dynamics simulations. J Biol Chem. 2005, 280 (1): 164-173.
Miyoshi S, Sasahara K, Akamatsu S, Rahman MM, Katsu T, Tomochika K, Shinoda S: Purification and characterization of a hemolysin produced by Vibrio mimicus. Infect Immun. 1997, 65 (5): 1830-1835.
Martin YC, Bures MG, Danaher EA, DeLazzer J, Lico I, Pavlik PA: A fast new approach to pharmacophore mapping and its application to dopaminergic and benzodiazepine agonists. J Comput Aided Mol Des. 1993, 7 (1): 83-102. 10.1007/BF00141577.
This work was supported by the State Key Program of Basic Research of China (973) (2002CB512802, 2002CB512803 and 2002CB428), the Hi-Tech Program of China (863) (2002AA229041, 2004AA223080), the Scientific Technology Development Foundation of Shanghai (02DJ14002, 055407069), and the National Natural Science Foundation of China (30400017). AD wishes to acknowledge support from the European Network of Excellence "Europathogenomics" LSHB-CT-2005-512061.
SM, AD, HJ and DQ conceived and designed the whole project. JZ performed the modeling of YycG protein and structure-based virtual screening for potential inhibitors, under the supervision of HJ. ZQ, BX, LC, YW and XY participated in the biological experiments about lead-compounds, XS, SM and DQ supervised the experiments. ZQ, JZ, LC, YW and AD analyzed the data and produced figures. ZQ, JZ, SM, AD, HJ, and DQ drafted the manuscript. All the authors have read and approved the final manuscript.
Zhiqiang Qin, Jian Zhang contributed equally to this work.
Electronic supplementary material
Additional File 1: Expression and purification of the recombinant YycG' protein. SDS-PAGE analysis of crude extracts from E. coli BL21 carrying pET28a (lane 2, prior to IPTG induction; lane 3, IPTG induction), pETYycG' (lane 4, prior to IPTG induction; lane 5, IPTG induction), purified YycG' (lane 6, approximately 34 kDa), and molecular weight standards were loaded in lane 1. (TIFF 774 KB)
Additional File 2: Measurement of kinase activity of YycG' protein in vitro. Luminescent output correlates with amount of ATP (A). A direct relationship exists between the luminescence measured with the Kinase-Glo™ Reagent and the amount of ATP. A constant amount of YycG' protein (4 μg) was added into reaction systems containing variant ATP concentrations (B). The respective reaction system without YycG' treatment was used as control. Variant amounts of YycG' protein was added into reaction systems containing a constant ATP concentration (50 μM). Each assay was performed in quadruplicate and repeated three times. The values represented the mean and SD of one separate experiment. (TIFF 505 KB)
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.