Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti
- Ye Zhang†1, 2,
- Laura Anne Smallbone†1,
- George C. diCenzo1,
- Richard Morton1 and
- Turlough M. Finan1Email author
© The Author(s). 2016
Received: 22 December 2015
Accepted: 15 July 2016
Published: 26 July 2016
Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. Despite the wide distribution of these enzymes, their biological roles are unclear in part because the reaction catalyzed by these enzymes can be by-passed by other pathways. The N2-fixing alfalfa symbiont Sinorhizobium meliloti contains both a NAD(P)-malic enzyme (DME) and a separate NADP-malic enzyme (TME) and to help understand the role of these enzymes, we investigated growth, metabolomic, and transcriptional consequences resulting from loss of these enzymes in free-living cells.
Loss of DME, TME, or both enzymes had no effect on growth with the glycolytic substrate, glucose. In contrast, the dme mutants, but not tme, grew slowly on the gluconeogenic substrate succinate and this slow growth was further reduced upon the addition of glucose. The dme mutant strains incubated with succinate accumulated trehalose and hexose sugar phosphates, secreted malate, and relative to wild-type, these cells had moderately increased transcription of genes involved in gluconeogenesis and pathways that divert metabolites away from the TCA cycle. While tme mutant cells grew at the same rate as wild-type on succinate, they accumulated the compatible solute putrescine.
NAD(P)-malic enzyme (DME) of S. meliloti is required for efficient metabolism of succinate via the TCA cycle. In dme mutants utilizing succinate, malate accumulates and is excreted and these cells appear to increase metabolite flow via gluconeogenesis with a resulting increase in the levels of hexose-6-phosphates and trehalose. For cells utilizing succinate, TME activity alone appeared to be insufficient to produce the levels of pyruvate required for efficient TCA cycle metabolism. Putrescine was found to accumulate in tme cells growing with succinate, and whether this is related to altered levels of NADPH requires further investigation.
Glucose is catabolized to pyruvic acid via the Embden-Meyerhof-Parnas (EMP) and/or the Entner-Doudoroff (ED) glycolytic pathways in many microorganisms. The pentose phosphate pathway is also used in both catabolic and anabolic metabolism. In aerobic organisms, pyruvate is oxidized via the tricarboxylic acid (TCA) cycle and the resulting reductant is passed-on to the electron acceptor O2 through an electron transport chain (ETC). This process generates an electrochemical proton potential that is used by the proton ATPase for ATP synthesis. When TCA cycle intermediates such as succinate or malate are employed as the carbon source, energy is generated via the TCA cycle and the ETC, while glucose and other sugars required for biosynthetic reactions are synthesized by a reversal of many of the reactions of the glycolytic pathways. This process is referred to as gluconeogenesis. Because several glycolytic reactions are not reversible, several new enzymes, such as phosphoenoylpyruvate (PEP) carboxykinase are synthesized in order for gluconeogenesis to occur. In both glycolysis and gluconeogenesis, the metabolic intermediates acetyl-CoA, pyruvate, PEP, 2-ketoglutarate, succinyl-CoA, and oxaloacetate are precursors for the synthesis of amino acids, nucleotides, lipids, and tetrapyrroles. The removal of these precursor compounds reduces flux through those pathways, and for flux through central carbon pathways to be maintained the lost intermediates are replenished via anaplerotic “fill-in” reactions catalyzed by enzymes such as pyruvate carboxylase, phosphoenolpyruvate carboxylase, and malic enzymes [1, 2].
Malic enzymes (ME) decarboxylate L-malate to pyruvate and reduce NAD(P)+ to NAD(P)H + H+ [3–5]. They link pathways of glycolysis and gluconeogenesis with the TCA cycle and play an anapleorotic role in replenishing metabolic intermediates and in the generation of NAD(P)H reductant . There is considerable variation in the distribution and the properties of MEs found in bacteria; some carry both NAD and NADP-dependent enzymes while other carry a single ME enzyme [3, 5, 7–9].
Rhizobia are free-living soil bacteria that form N2-fixing nodules on the roots of leguminous plants. The alfalfa symbiont Sinorhizobium meliloti has two MEs. DME is a diphosphopyridine nucleotide (NAD+)-dependent ME that also has some activity with NADP+ (EC 126.96.36.199), whereas TME is a strictly triphosphopyridine nucleotide (NADP+)-dependent ME, (EC 188.8.131.52) [3, 10–12]. The S. meliloti DME and TME proteins share similar kinetic properties (K m , V max ) for L-malate and their respective co-factors NAD+ or NADP+ respectively. DME activity is allosterically activated by malate, succinate, and fumarate, and is inhibited by acetyl-CoA, whereas TME activity does not appear to be regulated . DME, TME, and the Escherichia coli NADP+-dependent ME (maeB) are large 700 amino acids proteins. Their 400-amino-acid long N-terminal regions are responsible for the ME activity. The 300-amino-acid C- terminal domain is similar in sequence to phosphotransacetylase enzymes (PTA) but no PTA activity has been detected and the function of this large domain is unclear [3, 5].
The ME domain of the S. meliloti DME protein is required for N2-fixation in alfalfa nodules  and this requirement cannot be replaced through the production of the TME enzyme . The C4-dicarboxylates succinate and malate appear to be the primary carbon and energy sources used by the N2-fixing bacteria in the nodules [14–16], and it is thought that DME is required to synthesize high levels of pyruvate for use by pyruvate dehydrogenase (PDH) in the generation of acetyl-CoA to generate the energy necessary for the ATP intensive nitrogenase reaction [12, 16]. In some symbioses, pyruvate and acetyl-CoA can be produced through an alternate route catalyzed by the enzymes PEP-carboxykinase, pyruvate kinase, and PDH [17–19].
Here, we further investigated the biological roles of the DME and TME malic enzymes by investigating the transcription and polar metabolite profiles, and growth phenotypes of S. meliloti free-living dme and/or tme mutant cells. The findings are discussed with respect to the role of malic enzymes in central carbon metabolism and the role of DME in N2-fixing nodules.
Global metabolite analysis
TCA cycle intermediates accumulate in the DME mutant
However, the intracellular levels of malate and fumarate in succinate-grown dme and tme mutant cells were not significantly different (p < 0.05) (data not shown) presumably due to rapid export of accumulating metabolites. We therefore examined the intracellular profile of cells grown with succinate plus glucose as carbon sources. The GC-MS results showed that both malate and fumarate, as well as aspartate, accumulated to over twenty fold higher concentrations in dme cells than tme mutant cells (data not shown), suggesting that glucose addition exaggerates the build-up of TCA cycle intermediates.
Global transcriptional analysis
Transcriptional effects were investigated using whole genome microarrays similar to those previously used to identify genes involved in hydroxyproline metabolism and cobalt transport in S. meliloti [26, 27]. Hierarchical cluster analyses of the transcriptional profiles showed that the transcriptomes clustered first based on the growth media (glucose vs succinate), and then separately by strain, with differences between the wild-type, dme, and tme strains most pronounced when grown with succinate (Additional file 1: Figure S1).
Overview of the differential expression patterns based on gene function categoriesa
No. of genes significantly regulatedb (increased/decreased)
Glucose vs succinate (wt)c
dme vs wt (succinate)d
tme vs wt (succinate)e
dme vs wt (glucose)f
tme vs wt (glucose)g
Amino acid metabolism
Cofactor and vitamin metabolism
Fatty acid, ester, and phospholipid
Purine, pyrimidine, and nucleotide
DNA replication and repair
Gluconeogenic flux is increased in the DME mutant
Amino acid and fatty acid biosynthetic genes are up-regulated in the DME mutant
Rather surprisingly, a number of genes annotated as involved in the biosynthesis of fatty acids from acetyl-CoA were also up-regulated in the succinate-grown dme mutant cells (Fig. 4). The physiological and genetic basis for this increased transcription is less clear, as one might expect that the levels of the fatty-acid precursor acetyl-CoA to be reduced in dme mutant cells relative to wild-type when utilizing succinate. However, fatty acid biosynthesis involves NADPH as an electron donor , and thus, the increased fatty acid biosynthesis may be a response to reduce a build-up of NADPH reductant in the dme mutant that results from increased pyruvate synthesis by the NADP+ dependent TME malic enzyme.
DME mutations result in a growth phenotype with succinate
Transcription and metabolite data
The comparison of the transcription data for wild-type with dme or tme data sets revealed transcriptional changes, some of which were quite modest (Additional file 1: Figure S1). Evidence that these transcription differences are robust include the following: a) genes encoding different steps in the same metabolic pathway show similar co-regulation, b) genes present in operons showed similar co-regulation patterns (smc03978-81) (Figs. 3 and 4), c) when gusA or gfp reporter gene fusions to nine different transcripts  were assayed in wild type, dme, and tme backgrounds, the resulting gene fusion data showed a very good correlation with the microarray values (Additional file 1: Table S1), d) the transcription patterns observed for well characterized genes such as pckA, dctA, edd, dme, and tme in glucose vs succinate-grown cells were the same as those previously observed in S. meliloti using microarrays  reporter gene fusions and enzymes assays [19, 33–37].
To investigate whether the observed metabolic alterations were influenced by particular mutant strains or culture conditions, we examined the metabolic profiles of different dme and tme mutant cells and also cells harvested at different culture densities. These experiments revealed that the metabolite changes shown in Fig. 1 for dme and tme mutants were robust as they were observed in cells harvested at different culture densities (OD600 of 0.2 and 0.8) and from cultures carrying dme2 and dme3 and tme4 and tme2 mutant alleles (data not shown).
Overall, greater effects were observed with the dme mutation compared to the tme mutation, and the effects of both mutations were greater in cells utilizing succinate compared to glucose as carbon source. Aside from putrescine accumulation in succinate-grown cells, no clear metabolomic or gene expression patterns were identified and no growth phenotype was observed in the tme mutant strains (Figs. 1 and 5). On the other hand, succinate-grown cells of the dme mutant accumulated hexose sugar phosphates and trehalose and excreted large amounts of malate and fumarate (Figs. 1 and 2). These cells also showed increased transcription of gluconeogenic genes (Fig. 3) and dme mutant also displayed a growth defect when grown with succinate and the presence of glucose in addition to succinate exaggerated this growth phenotype (Fig. 5). Together, these data strongly suggest that the DME enzyme of S. meliloti maintains metabolic homeostasis and balances TCA cycle flux in cells that are utilizing a gluconeogenic substrate such as succinate as a source of carbon. We conclude that succinate utilization in cells lacking DME leads to malate accumulation, and that malate is removed from the TCA cycle either through excretion and/or by diverting TCA cycle intermediates through alternative pathways (i.e., gluconeogenesis and amino acid and fatty acid biosynthesis).
The role for DME balancing the TCA cycle metabolite flux is consistent with previously reported allosteric activation of DME activity by succinate, fumarate, and malate, and its potent inhibition by acetyl-CoA . DME and TME are present at similar activity levels in wild-type cells , and in concordance with previous observations, the transcript profile data revealed that the absence of one enzyme does not influence the expression of the other [10, 12]. The growth phenotype and metabolic data show that the active TME in dme mutant cells is insufficient to fully replace the physiological role of DME (Figs. 1 and 5). However, the physiological activity of TME does partially overlap with that of DME as dme tme double mutants had a reduced growth rate in the presence of succinate relative to single dme mutants (Fig. 5). This indicates that TME, and not PEP-carboxykinase and pyruvate kinase, serves as the main secondary pathway for pyruvate synthesis in succinate grown S. meliloti. These data are also consistent with previous suggestions that the role of TME is to maintain a basal malate to pyruvate flux that occurs even when DME is inhibited by high concentrations of acetyl-CoA [5, 10, 11].
The reduced growth phenotypes of free-living dme and the dme tme mutants with succinate (Fig. 5) appeared to be due to the accumulation of the TCA cycle intermediates, and not due to a lack of pyruvate. Pyruvate dehydrogenase (Pdh) is rapidly induced upon growth of S. meliloti on pyruvate , and because dme mutant cells grown with succinate showed a slight increase in transcription of genes encoding pyruvate kinase (Pyk) and Pdh relative to wild-type cells (Fig. 3), we suggest that the dme mutant cells synthesize substantial pyruvate. This would mean that the growth defect is likely not directly a consequence of a lack of pyruvate and a lack of ATP production, but rather a result of the accumulation of the TCA cycle intermediates. This hypothesis is supported by the observation that the reduced growth phenotype of succinate-grown dme and dme tme mutant strains is exaggerated if glucose is also present in the medium (Fig. 5); the presence of glucose may reduce the gluconeogenic flow of the mutant, increasing the accumulation of TCA cycle intermediates. The slow growth phenotype of the dme and dme tme mutants did not manifest itself until several hours following subculturing into succinate containing media (Fig. 5), perhaps as it takes several hours for the TCA cycle intermediates to reach inhibitory levels. It is also interesting to note that when grown with just succinate, the growth is logarithmic but slow, whereas in succinate + glucose, growth continuously gets slower. Thus, the glucose may be taken up slowly in the presence of succinate, and so does not have an effect early on, but as it is continuously taken up (and not metabolized), the growth continually gets poorer.
The diauxic growth phenotype observed in media containing succinate plus glucose is similar to the succinate mediated catabolite repression (SMCR) of lactose utilization that has been studied in some detail in S. meliloti . While the mechanistic details and identity of the major physiological effector molecule(s) responsible for SMCR remain to be fully elucidated, the relative levels of phosphoenolpyruvate and glutamine have been suggested to be involved in SMCR signalling . The dme or tme mutations did not appear to effect the levels of the SMCR signalling effector molecule(s) as the diauxic growth phenotype for dme and tme mutant strains in media containing succinate plus glucose was similar to the wild type (Fig. 5). While the metabolism of glucose by Sinorhizobium has been examined [41, 42], we are unaware of studies on the co-metabolism of succinate and glucose. Information on co-metabolism of these substrates, such as that available for Bacillus subtilis  would be valuable as while neither dme nor tme mutations effected the diauxic growth phenotype, the presence of glucose did effect the growth rate of the wild-type, dme, and tme strains on succinate (Fig. 5). These data suggest that there is some utilization of glucose during the first phase of growth (OD600 0.02–0.3) in media containing succinate plus glucose.
The accumulation of trehalose and putrescine in the succinate-grown dme, and tme mutant cells, respectively, suggests that these cells are stressed (Fig. 1). We note that accumulation of trehalose (and hexose-6-phosphates) in the dme mutant maybe an indirect consequence caused by the increased gluconeogenic flux resulting from the diversion of metabolites from the TCA cycle. The levels of putrescine and other polyamines have been shown to respond to osmotic stress in other bacteria [20–22]. The elevated level of putrescine observed in succinate-grown tme mutant cells was dependent on DME as the level of putrescine in the dme tme double mutant was similar to wild-type (Fig. 1). Because cellular concentrations of both putrescine and trehalose can vary in response to cell stress [23–25, 44, 45], the reduced level of putrescine in the tme dme double mutant may be related to the elevated level of trehalose. Additionally, the genes asnO and ngg were 4.0 and 5.2 fold up-regulated in the succinate-grown dme mutant compared to the wildtype (Fig. 4). These two genes form a pathway for the synthesis of the compatible solute N-acetylglutaminylglutamine amide (NAGGN) in S. meliloti . We observed no significant changes in dme versus wild type cells for the transcription of otsA or other genes implicated in trehalose synthesis in S. meliloti [treY (smb20574), treZ (smb21447), treS (smb20099)] [23, 24]. However in a related α-proteobacteria, R. leguminosarum bv. trifolii, trehalose accumulation appeared to be controlled by either post-transcriptional regulation or by control of breakdown rates . Similarly no significant changes were observed for the transcription of genes with possible links in putrescine synthesis (sma0680, sma0682, speB2)  in tme versus wild type cells. However putrescine is a substrate for the homospermidine synthase (Hss) enzyme , and the hss gene was highly transcribed under all conditions examined. The levels of putrescine in tme cells could be controlled by effectors of homospermidine synthase. In summary, further studies are required to investigate the linkage between trehalose, putrescine, and the malic enzyme mutations.
Symbiotic N2-fixing bacteroids in root-nodules catabolise plant derived C4-dicarboxylic acids as their major source of carbon and energy, and DME is required for symbiotic N2-fixation in alfalfa nodules. While DME activity can be bypassed in free-living cells by alternate pathways (i.e., TME activity and the combined Pck and Pyk activities) Pck activity is not detected in N2-fixing S. meliloti bacteroids and can therefore not complement dme mutations . The inability of TME to complement the loss of DME activity has remained an enduring question. The data reported here now provides insight into why this is the case: Nitrogenase has a high demand for ATP, requiring 16 moles of ATP per mole of fixed N2 . In order to produce the required supply of ATP, the succinate and malate provided by the plant cell must be rapidly converted to pyruvate for energy production. As shown here, TME is unable to convert malate to pyruvate at the rate that this is performed by DME, and the differences between TME and DME may even be further amplified during symbiosis because the NADP in the bacteroid is predominantly reduced. Thus, the symbiotic phenotype of a dme mutant is likely a consequence of insufficient pyruvate synthesized by TME for nitrogenase activity. Additionally, as N2-fixing bacteroids are osmotically sensitive and mutations that effect osmotic sensitivity have been reported to affect symbiotic performance , there is a possibility that the accumulation of TCA-cycle intermediates and stress associated with the loss of DME activity might also affect symbiotic N2-fixation.
A comparison of the S. meliloti dme or tme mutant phenotypes with those of malic enzyme mutants of E. coli or B. subtilis revealed large differences. Loss of both enzymes from E. coli had no effect on growth with glucose or acetate; but the double mutant exhibited a much extended adaptive lag before growing on acetate . The lag was attributed to a significant feedback interaction from metabolism to control of regulatory cAMP receptor protein (CRP) system. Of the four malic enzymes of B. subtilis, YtsJ reduces NADP+, whereas the three other enzymes reduce NAD+ (8.9). Mutations in these enzymes had no effect on growth with glucose or malate except for the NADP+-ME (ytsJ) mutant which grew poorly with malate. The authors suggested that YtsJ had a distinct function in the production of NADPH for biosynthetic purposes and that cells utilizing the gluconeogenic substrate malate do not produce sufficient NADPH by the pentose phosphate pathway. While these observations show that, not surprisingly, the phenotype of malic enzyme mutants is dependent on other pathways, it will be interesting to establish why the phenotype of NADP+-ME mutants of E. coli and S. meliloti is so different to that of B. subtilis.
NAD(P) malic enzyme (DME) is shown to play an important role in maintaining TCA cycle flux and balanced levels of TCA cycle intermediates. In cells utilizing succinate, the loss of DME lead to the accumulation and excretion of malate, and an increased gluconeogenic flow that presumably resulted in the observed accumulation of hexose-6-phosphates and trehalose. The dme mutant cells had a reduced rate of growth on succinate and this was exacerbated upon the addition of glucose. Succinate-grown tme mutant cells accumulated putrescine and the causative link for this affect requires further investigation.
Bacterial strains and culture media
Primary Sinorhizobium meliloti strains used in this study
S. meliloti SU47, str-21
Rm1021 dme-3::Tn5 tme-4::ΩSpr/Smr
Rm1021 with changed wild-type pstC
E. coli were grown at 37 °C in Luria-Bertani broth (LB), while S. meliloti cells were grown at 30 °C in LBmc (LB supplemented with 2.5 mM MgSO4 and 2.5 mM CaCl2). The defined M9 minimal medium used to grow S. meliloti cultures for polar metabolite analysis contained 1× M9 salts (Difco) supplemented with 0.25 mM CaCl2, 1 mM MgSO4, 0.5 μg mL-1 biotin, and 43 nM CoCl2, with 5–15 mM succinate and/or glucose as the carbon source [26, 50]. MOPS (morpholinpropanesulfonic acid) buffered minimal medium used to grow S. meliloti cultures for microarray assay contains 40 mM MOPS, 20 mM KOH, 20 mM NH4Cl, 1.2 mM CaCl2, 100 mM NaCl, 2 mM MgSO4, 2 mM KH2PO4, 0.5 μg mL-1 biotin, and 1× trace element solution, with 15 mM succinate or 15 mM glucose as the carbon source. The trace element solution (1000×) consisted of the following amounts of compounds per liter of H2O: 1 g H3BO3, 1 g ZnSO4 · 7H2O, 0.5 g CuSO4 · 5H2O, 0.5 g MnCl2 · 4H2O, 1 g Na2MoO4 · 2H2O, 10 g Na2EDTA · 2H2O, and 2 g NaFeEDTA. Concentrations of antibiotics (μg mL-1) for S. meliloti strains were as follows: streptomycin (Sm), 200; gentamicin (Gm), 60; spectinomycin (Sp), 200; and neomycin (Nm), 200. For E. coli Gm and Sp were added at 10 and 100 μg/ml respectively.
Microarray chips were purchased from NimbleGen Systems Inc., Madison,WI. RNA was extracted from 250 mL log phase cultures (OD600 of 0.4 to 0.8) of RmP110, RmP2179, and RmP2189 grown in MOPS-buffered minimal media with 15 mM succinate or 15 mM glucose. RNA extraction, cDNA end labelling with biotin, and hybridizations were done as previously described [26, 27]. The custom-made 4-plex arrays contained ~ 72 K probes of 60 oligonucleotides that targeted sequences within annotated start and end positions of 6269 annotated S. meliloti features, mostly protein-coding sequences but also some RNA sequences. Raw data probe intensities were quantile normalized across all experimental replicates (6 experiments × 2 replicates, or 12 arrays). Normalized probe intensities were subjected to a filter that required at least six non-redundant probes per feature. This reduced the number of features analysed to 6035. The median intensity of the pooled, filtered probes within an annotated region was used as an uncorrected measure of gene expression for each experiment. A set of 1786 null probes that did not match any S. meliloti genome sequence was used to estimate background for each array. Subtracting the array-estimated background from each feature median and averaging the two experimental replicates gave the average expression over each of the six experiments. Pair-wise experimental comparisons were made from the ratio of these background-corrected expression values and significance assessed using a Student’s t-test. The microarray data are available at Geo (http://www.ncbi.nlm.nih.gov/geo/) under the Accession Number GSE71308 and supplemental files (Additional file 1: Tables S2–S6 and Additional file 2: Dataset S1).
Polar metabolite analysis
For each strain, three to five single colonies were grown overnight in LBmc and subcultured (1:200) into 50 mL of M9 containing 15 mM glucose or 15 mM succinate in a 250 mL flask. These cultures were incubated at 30 °C with shaking (200 rpm) and grown to the desired OD600 (0.2 to 0.8). Cells were harvested by centrifugation (20 min at 3730 g), resuspended in 1 mL of distilled water, and flash frozen in liquid nitrogen then stored at −80 °C. For extraction, ribitol and NaCl were added to thawed cells to final concentrations of 3 ng mL-1 and 29 mM, respectively. The cells were then transferred to screw-cap tubes containing 0.75 mL of 0.1 mm glass beads (Biospec products) and 400 μL of 100 % methanol, and were then lysed by bead beating for three 1 min periods in a Mini Beadbeater-8 with 1 min incubation on ice following each beating. Following centrifugation (5 min at 14,000 g) and transfer of the supernatant to a new tube, the beads were washed with 1 mL of 60 % methanol and then with 400 μL of 100 % methanol. 750 μL of 100 % chloroform was added to the resulting supernatant fraction, and following centrifugation (5 min at 14,000 g) the resulting aqueous phase (~1.75 mL) was lyophilized.
For analysis of extracellular metabolites, the high concentration of phosphate (69 mM) in regular M9 media interfered with the GC-MS analysis as the phosphate is derivatized by MSTFA and the trimethylsilylized phosphate molecules produced very large peaks that occluded surrounding peaks. To alleviate this issue, cultures grown in M9 containing 7.5 mM glucose and 7.5 mM succinate were harvested at an OD600 0.5–0.6 and cells were centrifuged (20 min at 3730 g), washed with 0.85 % NaCl, and resuspended in a low phosphate minimal medium (20 mM NH4Cl, 2 mM MgSO4, 1.2 mM CaCl2, 100 mM NaCl, 0.5 μg mL-1 D-biotin, 0.01 μg mL-1 CoCl2, 2.5 mM KH2PO4 and 5 mM of the desired carbon source). Following incubation for 1–3.5 h, the cells were removed by centrifugation and 1 mL of each supernatant was flash frozen in liquid nitrogen and stored at −80 °C until needed. Ribitol was added to a final concentration of 3 ng mL-1, and prior to derivatization, the supernatant was filtered through Amicon Ultra Millipore columns to remove large molecular weight compounds (>10,000 Da). 200 μL of each sample was lyophilized overnight.
Methoxymation and silylation of metabolites were done using the procedures of Fiehn et al.,  and Roessner et al. , and samples were run on a Trace GC 2000/Trace DSQ (Thermo Finigan, Gerstel) equipped with a Rtx-5MS Integra-Guard column (Restek) with helium carrier gas. Retention times for fatty acids standards and the response factor for the internal standard ribitol was recorded for each replicate [53, 54] and following a statistical analysis of the data with ANOVA, significant compounds (P ≤ 0.05) were identified by retention time using the NIST library (National Institute of Standards and Technology). Relative response factors (RRF) for all peaks were calculated by dividing the response factor of the peak by the response factor for ribitol. To validate the identity of trehalose and putrescine, a co-injection with a known amount of an authentic standard was performed.
The concentration of L-malate in the extracellular medium was quantified enzymatically using the K-MALAF kit from Megazyme International Ireland Limited. A standard curve for L-malate was constructed with known concentrations of L-malate in the growth media.
Inoculum cultures grown in LBmc were washed once using carbon free M9 medium, then resuspended and diluted into M9 medium with the desired carbon source. Strains were grown in triplicate in 96-well microtitre plates in 150 μL cultures, and reported values are not corrected to a 1 cm pathlength. Growth conditions and analysis were as previously described .
DME, NAD(P)-malic enzyme; ED, Entner-Doudoroff; EMP, Embden-Meyerhof-Parnas; ETC, electron transport chain; Hss, homospermidine synthase; ME, malic enzyme; PDH, pyruvate dehydrogenase; PEP, phosphoenoylpyruvate; PTA, phosphotransacetylase enzyme; Pyk, pyruvate kinase; TCA, tricarboxylic acid; TME, NADP-malic enzyme
The authors are very grateful to Peter Summers, Elizabeth Weretilnyk, and Brian Golding for assistance with GC-MS metabolite analysis and Daniel Hsieh for comments on the manuscript.
This work was funded through grants to T.M.F. from the Natural Sciences and Engineering Research Council of Canada (NSERC) and Genome Canada and the Ontario Genomics Institute. G.C.D. is funded by NSERC through a NSERC CGS-D fellowship.
Availability of data and materials
Data supporting the results reported in the article can be found in the supplemental data and http://www.ncbi.nlm.nih.gov/geo/ under Accession GSE71308.
TMF participated in the design of the study, RM helped analyze the data, YZ, LAS, GCD carried out the experiments and with TMF participated in preparation of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
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