Fig. 5
Growth of wild-type strain, the dme, the tme and dme tme double mutants grown in M9-Glucose (5 mM), M9-Succinate (5 mM), or M9-Succinate (5 mM) plus Glucose (5 mM). OD600 values were from triplicate samples. The insert in the glucose + succinate panel, shows OD600 values between 0.2 and 0.8 over the part of the growth curves where first growth stops and the second growth resumes. Note, that because the strains entered the diauxic growth transition at quite different times, the insert graph plots the OD600 from 3 h prior to end of first growth phase to 3 h past end of first phase, allowing all growth curves to be aligned at the end of the first growth phase. Generation times in hours were determined at culture densities between OD600 0.1–0.3 (prior to the temporary halt in growth in the glucose + succinate cultures) and values are the means from the triplicate cultures +/− standard deviation of the mean. Both refers to media containing both succinate and glucose as carbon source