- Research article
- Open Access
Pilicides inhibit the FGL chaperone/usher assisted biogenesis of the Dr fimbrial polyadhesin from uropathogenic Escherichia coli
- Rafał Piatek†1Email author,
- Beata Zalewska-Piatek†1,
- Krystyna Dzierzbicka2,
- Sławomir Makowiec2,
- Justyna Pilipczuk1,
- Kasjan Szemiako1,
- Anna Cyranka-Czaja3 and
- Marek Wojciechowski4
© Piątek et al.; licensee BioMed Central Ltd. 2013
- Received: 31 October 2012
- Accepted: 4 June 2013
- Published: 12 June 2013
The global spread of bacterial resistance has given rise to a growing interest in new anti-bacterial agents with a new strategy of action. Pilicides are derivatives of ring-fused 2-pyridones which block the formation of the pili/fimbriae crucial to bacterial pathogenesis. They impair by means of a chaperone-usher pathway conserved in the Gram-negative bacteria of adhesive structures biogenesis. Pili/fimbriae of this type belong to two subfamilies, FGS and FGL, which differ in the details of their assembly mechanism. The data published to date have shown that pilicides inhibit biogenesis of type 1 and P pili of the FGS type which are encoded by uropathogenic E. coli strains.
We evaluated the anti-bacterial activity of literature pilicides as blockers of the assembly of a model example of FGL-type adhesive structures, – the Dr fimbriae encoded by a dra gene cluster of uropathogenic Escherichia coli strains. In comparison to the strain grown without pilicide, the Dr+ bacteria cultivated in the presence of the 3.5 mM concentration of pilicides resulted in a reduction of 75 to 87% in the adherence properties to CHO cells expressing Dr fimbrial DAF receptor protein. Using quantitative assays, we determined the amount of Dr fimbriae in the bacteria cultivated in the presence of 3.5 mM of pilicides to be reduced by 75 to 81%. The inhibition effect of pilicides is concentration dependent, which is a crucial property for their use as potential anti-bacterial agents. The data presented in this article indicate that pilicides in mM concentration effectively inhibit the adherence of Dr+ bacteria to the host cells, – the crucial, initial step in bacterial pathogenesis.
Structural analysis of the DraB chaperone clearly showed it to be a model of the FGL subfamily of chaperones. This permits us to conclude that analyzed pilicides in mM concentration are effective inhibitors of the assembly of adhesins belonging to the Dr family, and more speculatively, of other FGL-type adhesive organelles. The presented data and those published so far permit to speculate that based on the conservation of chaperone-usher pathway in Gram-negative bacteria , the pilicides are potential anti-bacterial agents with activity against numerous pathogens, the virulence of which is dependent on the adhesive structures of the chaperone-usher type.
- Polystyrene Microtitre Plate
- UPEC Strain
- Relative Adherence
- Periplasmic Chaperone
Bacterial pathogenesis is a complex process which has been well studied in the case of urinary tract infections (UTIs) mediated by uropathogenic Escherichia coli (UPEC) expressing type 1 and P pili. The crucial steps of this mechanism, namely, initial bacterial attachment, invasion and biofilm formation, are strictly dependent on the pili function [1, 2]. These structures belong to the family of adhesive organelles assembled in accordance with the classical chaperone-usher pathway, which is highly conserved in Gram-negative bacteria. Pili, fimbriae or amorphic adhesive oganelles are linear homo- or heteropolymers of hundreds to thousands of protein subunits. All these proteins possess a conserved immunoglobuline-like structure denoted by the lack of the seventh β-strand, G. The effect of this structural defect is a hydrophobic acceptor cleft flanked by the β-strands A and F [3–6]. The folding of protein subunits is strictly dependent on the action of the specific periplasmic chaperone protein. The chaperone complements the defective structure of a subunit by donating a specific G1 donor β-strand in line with the donor strand complementation (DSC) reaction [5–8]. The stable chaperone-subunit complex migrates to the usher protein located in the outer membrane, where the process of protein subunit polymerization occurs. The formation of the functional adhesive organelle propagates in accordance with the donor strand exchange (DSE) reaction This step is dependent on the action of the N-terminal donor peptide exposed from each subunit [9–11].
Though global conservation of chaperone, usher and fimbrial proteins, the available structural data describing the assembly of different adhesive organelles, namely, P and type 1 pili of E. coli, F1 surface antigen of Y. pestis, Dr/Afa-III fimbriae of E. coli, SAF fimbriae of S. typhimurium and colonization factor CS6 of E. coli, also identify many important differences between them [12–14]. The specific structural and functional adaptations connected with the biogenesis of particular adhesive structures have been well studied in the case of chaperone and subunit proteins. Sequence and structural data comparisons allow the family of periplasmic chaperones to be divided into two subfamilies on the basis of the length of the loop connecting β-strand F1 with the donor G1 strand, the FGL and FGS subfamilies having a long and a short loop, respectively [15, 16]. This loop is an important structural element which, in the chaperone-subunit complex, extends the acceptor cleft binding motif of the chaperone G1 donor strand. In the FGS chaperones, the β-strand G1 stabilizes a subunit core by donating only three bulky hydrophobic residues [4, 7]. In the case of FGL chaperones, the G1 binding motif is typically extended by two additional, bulky, alternating hydrophobic residues from a loop region [5, 13]. In the FGL chaperones, the second subunit-binding motif involved in the DSC mechanism is formed by three bulky hydrophopic residues located in the long N-terminal sequence forming the β-strand A1 [5, 13]. The long F1-loop-G1 hairpin of these chaperones is stabilized by the disulfide bond conserved in the whole subfamily [17, 18]. The longer G1 and A1 binding motif of the FGL chaperones correlates with the extended structure of the subunits’ acceptor cleft . The molecular differences in the structure and function of the FGL and the FGS chaperones presented here correlate with the structure of the adhesive organelles which they assemble . The FGL chaperones assemble organelles composed of only one type of protein subunit and, optionally, the second minor tip subunit [12, 13]. They are characterized by a thin fimbrial, amorphous or capsule-like morphology. Each subunit of these homopolymeric structures possesses the host-cell receptor binding site or sites; thus, they are polyadhesins. In contrast, the FGS chaperones assemble heteropolymeric, well-structured adhesive pili composed of up to seven different subunits [10, 19]. Pili are monoadhesins, as they possess only one receptor binding subunit located at the tip of the organelle. In addition, the division of chaperones and adhesive organelles into the FGS and FGL families also correlates with the phylogenetic analysis based on the usher ancestry. The FGL organelles belong to the γ3-monophyletic group, while the FGS can be divided into five clades: γ1, γ2, γ4, κ and π .
The adhesive organelles of the chaperone-usher type are unique virulence factors specific only to Gram-negative -pathogenic bacteria. The conservation of this mechanism renders it a good potential target for the development of antibacterial agents [21, 22]. The pilicides originally proposed by Svensson et al. in 2001 are a class of low molecular weight agents, derivatives of a dihydrothiazolo ring-fused 2-pyridone scaffold which block formation of pili by affecting the function of chaperone . According to the crystallographic and biological data, pilicides target the chaperone-usher pathway by blocking interaction between the N-terminal domain of the usher and chaperone-subunit complex. Hence, the pilicides block the formation of pili by preventing a DSE reaction. Pilicides bind to the hydrophobic patch of residues located in the F1, C1, D1 region of the N-terminal domain conserved in all chaperones . This region encompasses part of the F1-G1 loop which is structurally rearranged during the formation of the chaperone-subunit complex (DSC reaction). The dynamic nature of this region is also reflected in the pilicide binding modes observed in the crystal structures of the pilicide in the complex with a free PapD chaperone or the PapD-PapH complex [23, 24]. Although, pilicide interactions with conserved I93, located at the end of the β-strand F1, with L32 and with the V56 patch are preserved in these two structures, the electrostatic interactions between R96, located within the loop F1-G1, and R58 residues and carboxyl and carbonyl groups of pilicide are broken as a consequence of the PapH binding to the PapD . The important differences in the structure of the F1-G1 hairpin and the mechanism of DSC reaction observed between the FGS and FGL assembly systems might potentially affect pilicide binding. This gives rise to the question as to whether pilicides that were originally designed on the basis of the structure of the FGS-type PapD and FimC chaperones and were evaluated as inhibitors of the biogenesis of the P and type 1 pili are also active in respect of the FGL assembly pathway.
In this study, we addressed a question denoting the activity of pilicides as inhibitors of the assembly of the Dr fimbriae encoded by the dra operon of uropathogenic E. coli - the model of the FGL-type adhesive structures [25, 26]. These organelles are homopolymers of a single DraE subunit, the structure of which has three receptor binding sites interacting with the following host-cell molecules: Dra blood-group antigen presented on the CD55/decay-accelerating factor (DAF), the carcinoembryonic antigen (CEA)-related cellular adhesion molecules and the 7S domain of basement membrane protein type IV collagen [27–29]. The assembly of Dr fimbriae is dependent on the action of the DraB chaperone and the DraC usher . The data presented in this article are also important from the epidemiology point of view, as uropathogenic E. coli Dr+ strains are responsible for 20–25% of cases of cystitis and 30% of pyelonephritis in pregnant woman .
General synthesis of pilicides
The reagents were purchased from Sigma-Aldrich. The analytical TLC was performed on aluminum sheets of silica gel UV-254 (Merck). The flash chromatography was carried out using Zeochem silica gel with particle size of 40–63 microns. The NMR spectra 1H and 13C were recorded at Varian Gemini 200 and Varian Unity Plus 500 in CDCl3 or DMSO. The melting points are uncorrected. Pilicides 1 and 2 were prepared in accordance with previously published procedures [22, 31]. Given the poor water solubility of the acidic forms of these pilicides, their lithium salts were used in all the experiments. The resulting solutions of compounds were frozen and lyophilized. In order to conduct the experiments, the pilicides were initially dissolved in pure DMSO and the final concentration of DMSO in the growth media was 5%.
In the case of E. coli Dr+ strain adherence to CHO cells assay and collagen binding assay the statistical significance of results was tested using one-way ANOVA (p-value threshold = 0.05). Influence of pilicides 1 and 2 concentration on the bacterial adherence to CHO cells was assessed relatively to positive control means experiments with adherence of BL21DE3/pBJN406 strain cultivated without pilicide to CHO-DAF+ cells. Influence of pilicide 1 concentration on bacterial binding to the polystyrene microtitre plates coated with type IV collagen was assessed relatively to positive control means experiments with BL21DE3/pBJN406 strain cultivated without pilicide.
Bacterial strains and plasmids
The following E. coli strains were used: BL21DE3/pBJN406 – the strain encoding within the pBJN406 plasmid the wild type dra operon from the clinical UPEC IH11128 strain, the plasmid is a derivative of the pACYC184 vector; BL21DE3/pACYC184 – a strain used as Dr-type, non-fimbriated, negative control [26, 32]. In order to select for the presence of these plasmids, bacteria were grown on media supplemented with chloramphenicol at a concentration of 34 μg/ml.
Assay of E. coli Dr+strain adherence to CHO cells
CHO cells (Chinese hamster ovary K-1) and CHO-DAF+ cells stably transfected with cDNA for human DAF  were cultured in Ham’s F12 medium supplemented with 10% (vol/vol) fetal bovine serum (Sigma) and a penicillin-streptomycin solution (Sigma) in a 5% CO2 atmosphere at 37°C. The cell lines were passaged using 0.25% (vol/vol) trypsin containing EDTA (Sigma). For the adherence assay, the CHO-DAF+ and the CHO-DAF- cells were split into 6-well plates with glass coverslips, and grown in the appropriate medium for 18 h. Before the assay, the CHO cells were washed twice with phosphate buffered saline (PBS) and incubated with fresh medium, without antibiotics and without FBS for 1 h. The E. coli BL21DE3/pBJN406 strain was cultivated with shaking in Luria-Bertani (LB) medium, supplemented with chloramphenicol, for 24 h at 37°C. 100 μl of the bacterial culture was then split on TSA (trypticase soy agar) plates containing 5% DMSO, chloramphenicol and either supplemented or not with 0.5, 1.5, 2.5 and 3.5 mM pilicide 1 and 2 for another 24 h at 37°C. As the negative control the E. coli BL21DE3/pACYC184 strain cultivated on TSA plates not supplemented with pilicides was used. The overnight bacterial strains were harvested from plates washed twice with PBS and resuspended in this buffer to a final OD600 of 1.5. 50 μl of each of the E. coli bacterial strain suspensions were added to the 4 wells containing the CHO-DAF+ or CHO-DAF- cells grown on glass coverslips and incubated for 1.5 h at 37°C. The wells were then washed three times with PBS, fixed with 70% methanol and stained with 10% Giemsa in order to visualize the bound bacteria. Finally, the glass coverslips were examined for bound bacteria under an Olympus inverted microscope (CKX41) with phase-contrast objective. From each coverslip 40 CHO cells were examined and associated bacteria were counted. For each combination of the bacterial strain and CHO cell culture three independent experiments were carried out. To avoid experimenter random errors each experiment was performed using fresh bacterial transformants, fresh CHO cells cultures and fresh preparation of growth media. In all experiment for each combination of the bacterial strain and CHO cell culture four replicates were performed. As a result for each analyzed combination set of twelve data were obtained and analyzed statistically. The obtained values of adherences are expressed as the percentage of mean value of adherence present relative to the CHO-DAF+ positive control assay, with a standard deviation because in this form they are more meaningful and easier to compare with the published data.
The bacteria were cultivated on TSA plates either supplemented or not with 3.5 mM pilicide, in exactly the same way as for the CHO cells’ adherence assay. The bacteria were scraped from the plates, washed and suspended in PBS buffer to a final OD600 of 1.0. These bacterial preparations were used in haemagglutination assays in order to evaluate their level of fimbriation. The human erythrocytes were prepared from blood group O, the whole blood having been donated by a healthy volunteer. The erythrocytes were washed three times with PBS and then suspended in a PBS containing 2% D-mannose to a final OD640 of 1.4. The serial dilutions of the bacteria were prepared on 12-well microtitre plates. The mannose resistant haemagglutination (MRHA) assay was performed by adding an equal volume of the erythrocyte suspension to the wells containing bacterial serial dilutions. The haemagglutination experiments were conducted on ice. The last well containing agglutination was visually determined. The HA-titer denotes the inverse of the latest bacterial dilution which still provides agglutination. To confirm that the agglutination observed is an effect of the interaction between the Dr fimbriae and DAF receptor, the reversibility of this reaction as a consequence of chloramphenicol addition to a 2 μM final concentration was monitored. The HA-titers were an average determined from duplicate runs in three independent experiments.
Collagen binding assay
The wells of the polystyrene microtitre plate were coated with type IV collagen from human placenta (Sigma) at a concentration of 20 mg/ml and incubated at 4°C overnight. They were then washed three times with PBS and blocked with 1% BSA in PBS for 2 h at 37°C. After the removal of the blocking buffer, 100 μl of bacterial cells suspended in a PBS adjusted to a final OD600 of 1.0 were added to the collagen-coated coverslips and incubated for another 2 h at 37°C. Additionally, the bacterial preparations were diluted 1:1, 1:2, 1:4, 1:6 and 1:8 in PBS. The bacteria used in the assay were cultivated overnight with shaking in the LB medium (5% DMSO, chloramphenicol), either supplemented or not with 0.5, 1.5, 2.5 and 3.5 mM pilicide 1 for 24 h at 37°C. The Dr fimbriae of the bacteria bound to the collagen were detected with rabbit polyclonal anti-Dr (Immunolab, Poland) and goat anti-rabbit IgG-HRP (Sigma) antibodies at dilutions of 1:500 and 1:5000, with incubation for 40 min at 37°C, respectively. All the antibodies were diluted in a PBS containing 0.2% BSA. The bound antibodies were quantified using Sigma Fast o-phenylenediamine substrate (Sigma) as per manufacturer’s instructions, and measured in an ELISA plate reader (Victor3V, PerkinElmer) at a 490 nm wavelength. The experiment was performed at least three times in duplicate using fresh bacterial transformations and the mean value with standard deviation was determined.
Densitometry analysis of SDS-PAGE resolved fimbrial fractions
Dr fimbrial fractions were isolated from E. coli BL21DE3/pBJN406 grown for 24h on TSA plates (5% DMSO, chloramphenicol) in the presence of 0, 0.5, 1.5, 2.5 and 3.5 mM pilicides 1 and 2. As a control experiment, a fimbrial fraction was isolated from a non-fimbriated BL21DE3/pACYC184 strain cultivated without pilicide. The bacterial cells were centrifuged (14,000xg), resuspended in a PBS to OD600 of 1.0 and vigorously vortexed for 15 min at ambient temperature. The cellular suspensions were then centrifuged (14,000xg) and the supernatants containing the bacterial fimbrial fractions were collected and stored at 4°C. The same volumes (20 μl) of analyzed samples were mixed with Laemmli sample buffer (5 μl), denatured at 100°C for 60 min and ran in 15% (w/v) bis-acrylamide gels containing SDS. To ensure that all the Dr fimbriae were denatured to a monomeric DraE protein, a parallel Western blotting with rabbit anti-Dr serum was conducted. The proteins separated by gel electrophoresis were visualized using Coomasie blue staining. The relative concentration of DraE protein in the fimbrial fractions was determined by means of a densitometry analysis conducted with an SDS-PAGE low-molecular-weight calibration kit (GE Healthcare, Little Chalfont, UK) as a standard, using a VersaDoc system with Quantity One software (both from Bio-Rad, Hercules, CA). The reference E. coli BL21DE3/pBJN406 grown without pilicide arbitrary was set to 100%. The experiment was performed three times using fresh bacterial transformations. The summated optical density for the average of the analyzed bands was densitometrically determined from the three measurements for each experiment. For the technical reasons only three experiments were performed and the standard deviation of the mean value was not determined.
Rationale for the choice of pilicides
Pilicides block Dr fimbriae-dependent bacterial adherence
At the first stage, we determined the adherence of bacteria cultivated on TSA plates in the presence of 0.5, 1.5, 2.5 and 3.5 mM of pilicides 1 and 2 to the CHO cells transfected with plasmid encoding DAF receptor protein recognized by Dr fimbriae. The process of bacteria attachment was visualized by means of Giemsa staining. In the case of strain BL21DE3/pBJN406 cultivated without pilicide (positive control), we observed a high level of bacteria attachment to the CHO-DAF+ cells related to the undisturbed production of Dr fimbriae (Figure 1I). The adherence of positive control is set as 100% ±12 and the observed adherences of all other used bacterial preparations are expressed as the percentage of mean value of adherence present relative to control. The addition of 3.5 mM pilicide to the bacterial growth media resulted in a very high reduction in the bacterial adhesion properties: for pilicide 2, only a few bacterial cells were visible as attached, corresponding to the relative bacterial adherence of 13% ±3 (Figure 1B) and for pilicide 1 resulted in a slightly lower inhibition of bacterial attachment, corresponding to the relative adherence of 25% ±7 (Figure 1A). E. coli BL21DE3/pBJN406 bacterial strains cultivated in the presence of 0.5, 1.5 and 2.5 mM of pilicides 1 and 2 showed dose dependent relative adherence of: 90% ±3, 60% ±5 and 32% ±6 for pilicide 1 and 92% ±8, 42% ±7 and 21% ±9 for pilicide 2, respectively (Figure 1 G,E,C and H,F,D). In order to confirm that the bacterial adherence is dependent on the specific interactions between the DraE fimbrial subunits and DAF, we used as the control non-transfected CHO cells, which do not express DAF molecules naturally. The relative adherence of Dr-fimbriated BL21DE3/pBJN406 positive control (Figure 1J), non-fimbriated BL21DE3/pACYC184 negative control (Figure 1L) and BL21DE3/pBJN406 strain grown in the presence of 3.5 mM of pilicide 1 or 2 (Figure 1M and N) to the CHO-DAF- cells for all experiments was of 3-6% ± 1–2. The similar value of relative adherence of 5% ±6 was determined for binding of non-fimbriated BL21DE3/pACYC184 negative control strain to CHO-DAF+ cells. Statistical analysis confirmed the significance of decreased adherence (p < 0.05) after the exposure of bacteria on different concentration of pilicides. Only the result for the lowest concentration of pilicide 2 was statistically not significant relatively to the positive control (p = 0.068). The increasing concentration of pilicides also had the influence on adhesion level (p < 0.05).
Pilicide-treated bacteria possess a reduced quantity of Dr fimbriae
To further evaluate the effect of pilicides on the inhibition of Dr fimbriae production, we quantified the amount of monomeric DraE protein resulting from the denaturation/depolimerization of isolated Dr fimbriae samples using a densitometric analysis of the SDS-PAGE gels stained with Coomassie Blue (Figure 3A-C). The strain E. coli BL21DE3/pBJN406 was grown on agar plates supplemented with pilicides 1 and 2 at a concentration of 0.5, 1.5, 2.5 and 3.5 mM. Non-fimbriated E. coli BL21DE3/pACYC184 and fully-fimbriated BL21DE3/pBJN406 strains grown without pilicide were used as the negative and positive controls, respectively. The fimbriae from the bacteria scraped and normalized to OD600 were isolated by means of vortexing. Dr fimbriae are very stable structures which require extending heating in Laemmli buffer in order to depolimerize to a monomeric DraE protein. The band of monomeric DraE protein was visible in resolved samples heated for 60 min at 100°C before electrophoresis. In contrast, there was no band corresponding to monomeric DraE in the samples which had not been denatured thermally before electrophoresis (Figure 3A). This confirms that the isolated fractions only contained Dr fimbriae and were not contaminated by the monomeric, periplasmic form of DraE protein. In order to prove that the heating time for the samples is sufficient for the total denaturation of Dr fimbrial structures to monomeric DraE, we performed a Western blotting analysis with anti-Dr antibodies (Figure 3B). In these experiments, the estimated pilicide effects of compounds 1 and 2 were comparable (Figure 3C). For bacteria cultivated in the presence of 3.5 mM of pilicides 1 and 2, the amount of DraE fimbrial protein was reduced by 75 and 80% in comparison to the fully-fimbriated strain grown without pilicide, respectively. Performing experiments with 0.5, 1.5 and 2.5 mM concentration of pilicides, we analyzed their dose dependent effects on the volume of fimbrial production. At a concentration of 2.5 mM, pilicides 1 and 2 inhibit fimbrial production at comparable level as in the case of bacteria treated with the 3.5 mM concentration, – adherence reduction by 68 and 75%, respectively. The use of pilicides 1 and 2 at a concentration of 1.5 mM results in a relative DraE reduction of 55 and 45%, respectively. Bacteria cultivated with 0.5 of pilicides 1 and 2 have almost the same amount of the DraE protein derived from Dr fimbriae as in the case of strain grown without the addition of the compounds, – adherence reduction 8 and 3%, respectively.
Activity of pilicides 1 and 2 as inhibitors of Dr fimbriae biogenesis was tested on the E. coli BL21DE3/pBJN406 – the laboratory model of the clinical UPEC IH11128 strain. Biological evaluations based on the whole-cell assays were predominantly performed using a 3.5 mM concentration of pilicides, as is used in the case of most experiments with an inhibition of type 1 and P pili formation. The E. coli BL21DE3/pBJN406 bacteria cultivated in the presence of 3.5 mM pilicides 1 and 2 showed the amount of DraE subunits/Dr fimbriae reduced by 75–80% as determined by SDS-PAGE densitometry analysis of isolated fimbrial fractions. A Western immunoblot analysis of this strain with anti-Dr antibodies denoted a reduction, by 81%, of the amount of Dr fimbriae in relation to fully-fimbriated, pilicide untreated bacteria. The amounts of major pili P PapA (recombinant strain HB101/pPAP5) and type 1 pili FimA (clinical strain UTI89) subunits isolated from bacteria cultivated in the presence of 3.5 mM of pilicide 1 analyzed by immunoblot were reduced by 68% and 53%, respectively [23, 36]; in the case of FimA, the C-6 morpholinomethyl substituent in pilicide 1 with no effects on its biological activity was compared. The atomic force microscopy analysis of the HB101/pPAP5 strain showed that the bacteria treated with 3.5 mM of pilicide 1 were devoid of P pili . The inhibition of Dr fimbriae production by 3.5 mM pilicides 1 and 2 is reflected in the 25% ± 7 and 13 ±3% DAF dependent bacteria relative adherence to CHO cells, respectively. This correlates well with the 90% reduction in adherence to the bladder cells of E. coli NU14 producing type 1 pili cultivated in the presence of a C-6 morpholinomethyl derivative of pilicide 1. In the haemaglutynation assay, which also reflects the adherence properties of E. coli BL21DE3/pBJN406 Dr+ strain treated with 3.5 mM pilicides 1 and 2, we observed an HA-titer of 16/32; the strain untreated with pilicide constituting the control has an HA-titer of 128. Published HA-titer data for the HB101/pPA5A strain, treated and untreated with pilicide 1, are 1/4 and 128, respectively [34, 36]. The difference observed in the HA-titer of the pilicide-treated Dr- and pili P-producing strains is difficult to analyze because of experimental reasons. We only presumed that the higher haemagglutination properties of Dr fimbriae-producing bacteria might be connected with the polyadhesin nature of these structures, in contrast to the monoadhesin of P pili. As the DraE subunits are multi-receptor adhesins, the inhibition of Dr fimbriae assembly by pilicides was also confirmed by the evaluation of bacterial adherence to the type IV human collagen receptor. The SDS-PAGE analysis of isolated fimbrial fractions, collagen binding assay and Dr fimbriae dependent bacterial adherence to CHO-DAF+ cells assay performed using bacteria cultivated in the 0, 0.5, 1.5, 2.5 and 3.5 mM of compounds 1 and 2 confirmed that the effect of Dr fimbriae assembly inhibition observed was dependent on the pilicide concentration used. This is a crucial feature of the antibacterial agents. The data based on the whole cell assays presented in this article confirm that pilicides effectively inhibit the receptor-dependent adherence of E. coli Dr+ strain to the host cells. Thus pilicides impair the crucial step of bacterial pathogenesis, namely, – the formation of initial, close contact between bacteria and host cell. The evaluations of the pilicides’ effects on E. coli Dr+ strain are comparable to those previously published for type 1- and P pili-producing bacteria. This suggests that the structural and functional differences observed between FGS and FGL chaperone-usher systems are not crucial to pilicide activity. This thesis is supported by the structure of the Caf1-Caf1M subunit-chaperone pre-assembly complex bound to the N-terminal domain of Caf1A usher – the example of the FGL system . Although Caf1A and FimD belong to the FGL and FGS subfamilies of usher respectively, their N-terminal domains represents a high degree of structural similarity. The structures of usher binding sites that encompass pilicide binding residues are also highly conserved in the FGL and FGS type chaperones (Figure 4B). Comparison of the free Caf1M and Caf1-Caf1M complex structures permits to identify in the usher binding site of Caf1M chaperone specific “proline lock” that by interaction with Caf1 subunit allostericaly controls the chaperone-usher pathway . Such ”proline lock” was also identified in the available sequences and structures of usher binding sites of the other FGS and FGL type chaperones including DraB (Figure 4B) . This clearly shows that interaction between N-terminal domain of usher and usher binding motif of chaperones is highly conserved structurally and mechanically.
We conclude that pilicides 1 and 2 in mM concentration effectively inhibit the adherence of the laboratory model of uropathogenic E. coli Dr+ strain, - the main causative agent of cystitis and pyelonephritis in pregnant women, to the host cell DAF and collagen receptors by blocking the assembly of Dr fimbriae. On the basis of the high conservation of the Dr family of adhesins, which include clinically important human UPEC strains, we deduce that the pilicides should also effectively inhibit their assembly and, more speculatively, other FGL adhesive structures. The earlier thesis proposed by pilicide originators: “Pilicides, by blocking chaperone and usher function, have the potential to inhibit pili formation in a broad spectrum of pathogenic bacteria to prevent critical host-pathogen interactions necessary for many diseases ” has been considerably reinforced experimentally by extending the examination of pilicide activity from FGS-type structures to the assembly of FGL-type Dr fimbriae.
This work was supported by the Ministry of Science and Higher Education, grants number: N N401 221834 and N N401 569438. Thanks to prof. Bogdan Nowicki for supplying pBJN406 plasmid.
- Justice SS, Hung C, Theriot JA, Fletcher DA, Anderson GG, Footer MJ, Hultgren SJ: Differentiation and developmental pathways of uropathogenic Escherichia coli in urinary tract pathogenesis. Proc Natl Acad Sci USA. 2004, 101: 1333-1338. 10.1073/pnas.0308125100.PubMedPubMed CentralView ArticleGoogle Scholar
- Wright KJ, Seed PC, Hultgren SJ: Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili. Cell Microbiol. 2007, 9: 2230-2241. 10.1111/j.1462-5822.2007.00952.x.PubMedView ArticleGoogle Scholar
- Sauer FG, Futterer K, Pinkner JS, Dodson KW, Hultgren SJ, Waksman G: Structural basis of chaperone function and pilus biogenesis. Science. 1999, 285: 1058-1061. 10.1126/science.285.5430.1058.PubMedView ArticleGoogle Scholar
- Choudhury D, Thompson A, Stojanoff V, Langermann S, Pinkner J, Hultgren SJ, Knight SD: X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli. Science. 1999, 285: 1061-1066. 10.1126/science.285.5430.1061.PubMedView ArticleGoogle Scholar
- Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD: Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation. Cell. 2003, 113: 587-596. 10.1016/S0092-8674(03)00351-9.PubMedView ArticleGoogle Scholar
- Zavialov AV, Tischenko VM, Fooks LJ, Brandsdal BO, Aqvist J, Zav'yalov VP, Macintyre S, Knight SD: Resolving the energy paradox of chaperone/usher-mediated fibre assembly. Biochem J. 2005, 389: 685-694. 10.1042/BJ20050426.PubMedPubMed CentralView ArticleGoogle Scholar
- Barnhart MM, Pinkner JS, Soto GE, Sauer FG, Langermann S, Waksman G, Frieden C, Hultgren SJ: PapD-like chaperones provide the missing information for folding of pilin proteins. Proc Natl Acad Sci USA. 2000, 97: 7709-7714. 10.1073/pnas.130183897.PubMedPubMed CentralView ArticleGoogle Scholar
- Sauer FG, Pinkner JS, Waksman G, Hultgren SJ: Chaperone priming of pilus subunits facilitates a topological transition that drives fiber formation. Cell. 2002, 111: 543-551. 10.1016/S0092-8674(02)01050-4.PubMedView ArticleGoogle Scholar
- Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G: Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism. Mol Cell. 2006, 22: 831-842. 10.1016/j.molcel.2006.05.033.PubMedView ArticleGoogle Scholar
- Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H: Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell. 2008, 133: 640-652. 10.1016/j.cell.2008.03.033.PubMedPubMed CentralView ArticleGoogle Scholar
- Di Yu X, Dubnovitsky A, Pudney AF, Macintyre S, Knight SDAVZ: Allosteric mechanism controls traffic in the chaperone/usher pathway. Structure. 2012, 20: 1861-1871. 10.1016/j.str.2012.08.016.PubMedView ArticleGoogle Scholar
- Zavialov A, Zav'yalova G, Korpela T, Zav'yalov V: FGL chaperone-assembled fimbrial polyadhesins: anti-immune armament of Gram-negative bacterial pathogens. FEMS Microbiol Rev. 2007, 31: 478-514. 10.1111/j.1574-6976.2007.00075.x.PubMedView ArticleGoogle Scholar
- Zav'yalov V, Zavialov A, Zav'yalova G, Korpela T: Adhesive organelles of Gram-negative pathogens assembled with the classical chaperone/usher machinery: structure and function from a clinical standpoint. FEMS Microb Rev. 2010, 34: 317-378. 10.1111/j.1574-6976.2009.00201.x.View ArticleGoogle Scholar
- Roy SP, Rahman MM, Yu XD, Tuittila M, Knight SD, Zavialov A: Crystal structure of enterotoxigenic Escherichia coli colonization factor CS6 reveals a novel type of functional assembly. Mol Microbiol. 2012, 86: 1100-1115. 10.1111/mmi.12044.PubMedView ArticleGoogle Scholar
- Hung DL, Knight SD, Woods RM, Pinkner JS, Hultgren SJ: Molecular basis of two subfamilies of immunoglobulin-like chaperones. EMBO J. 1996, 15: 3792-3805.PubMedPubMed CentralGoogle Scholar
- Zav'yalov VP, Zav'yalova GA, Denesyuk AI, Korpela T: Modelling of steric structure of a periplasmic molecular chaperone Caf1M of Yersinia pestis, a prototype member of a subfamily with characteristic structural and functional features. FEMS Immunol Med Microbiol. 1995, 11: 19-24. 10.1111/j.1574-695X.1995.tb00074.x.PubMedView ArticleGoogle Scholar
- Piątek R, Zalewska B, Kolaj OMF, Nowicki B, Kur J: Molecular aspects of biogenesis of Escherichia coli Dr Fimbriae: characterization of DraB-DraE complexes. Infect Immun. 2005, 73: 135-145. 10.1128/IAI.73.1.135-145.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Zav'yalov VP, Chernovskaya TV, Chapman DA, Karlyshev AV, MacIntyre S, Zavialov AV, Vasiliev AM, Denesyuk AI, Zav'yalova GA, Dudich IV, et al: Influence of the conserved disulphide bond, exposed to the putative binding pocket, on the structure and function of the immunoglobulin-like molecular chaperone Caf1M of Yersinia pestis. Biochem J. 1997, 324: 571-578.PubMedPubMed CentralView ArticleGoogle Scholar
- Jonson AB, Normark S, Rhen M: Fimbriae, pili, flagella and bacterial virulence. Contrib Microbiol. 2005, 12: 67-89.PubMedView ArticleGoogle Scholar
- Nuccio SP, Bäumler AJ: Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek. Microbiol Mol Biol Rev. 2007, 71: 551-575. 10.1128/MMBR.00014-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Aberg V, Almqvist F: Pilicides-small molecules targeting bacterial virulence. Org Biomol Chem. 2007, 5: 1827-1834. 10.1039/b702397a.PubMedView ArticleGoogle Scholar
- Svensson A, Larsson A, Emtenäs H, Hedenström M, Fex T, Hultgren SJ, Pinkner JS, Almqvist F, Kihlberg J: Design and evaluation of pilicides: potential novel antibacterial agents directed against uropathogenic Escherichia coli. Chembiochem. 2001, 2: 915-918. 10.1002/1439-7633(20011203)2:12<915::AID-CBIC915>3.0.CO;2-M.PubMedView ArticleGoogle Scholar
- Pinkner JS, Remaut H, Buelens F, Miller E, Aberg V, Pemberton N, Hedenström M, Larsson A, Seed P, Waksman G, et al: Rationally designed small compounds inhibit pilus biogenesis in uropathogenic bacteria. Proc Natl Acad Sci USA. 2006, 103: 17897-17902. 10.1073/pnas.0606795103.PubMedPubMed CentralView ArticleGoogle Scholar
- Chorell E, Pinkner JS, Phan G, Edvinsson S, Buelens F, Remaut H, Waksman G, Hultgren SJ, Almqvist F: Design and synthesis of C-2 substituted thiazolo and dihydrothiazolo ring-fused 2-pyridones: pilicides with increased antivirulence activity. J Med Chem. 2010, 12: 5690-5695.View ArticleGoogle Scholar
- Anderson KL, Billington J, Pettigrew D, Cota E, Simpson P, Roversi P, Chen HA, Urvil P, du Merle L, Barlow PN, et al: An atomic resolution model for assembly, architecture, and function of the Dr adhesins. Mol Cell. 2004, 15: 647-657. 10.1016/j.molcel.2004.08.003.PubMedView ArticleGoogle Scholar
- Nowicki B, Barrish JP, Korhonen T, Hull RA, Hull SI: Molecular cloning of the Escherichia coli O75X adhesin. Infect Immun. 1987, 55: 3168-3173.PubMedPubMed CentralGoogle Scholar
- Berger CN, Billker O, Meyer TF, Servin AL, Kansau I: Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC). Mol Microbiol. 2004, 52: 963-983. 10.1111/j.1365-2958.2004.04033.x.PubMedView ArticleGoogle Scholar
- Nowicki B, Moulds J, Hull R, Hull S: A hemagglutinin of uropathogenic Escherichia coli recognizes the Dr blood group antigen. Infect Immun. 1988, 56: 1057-1060.PubMedPubMed CentralGoogle Scholar
- Westerlund B, Kuusela P, Risteli J, Risteli L, Vartio T, Rauvala H, Virkola R, Korhonen TK: The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen-binding protein. Mol Microbiol. 1989, 3: 329-337. 10.1111/j.1365-2958.1989.tb00178.x.PubMedView ArticleGoogle Scholar
- Servin AL: Pathogenesis of Afa/Dr diffusely adhering Escherichia coli. Int J Med Microbiol. 2005, 295: 471-478. 10.1016/j.ijmm.2005.07.001.View ArticleGoogle Scholar
- Aberg V, Hedenström M, Pinkner JS, Hultgren SJ, Almqvist F: C-Terminal properties are important for ring-fused 2-pyridones that interfere with the chaperone function in uropathogenic E. coli. Org Biomol Chem. 2005, 3: 3886-3892. 10.1039/b509376g.PubMedView ArticleGoogle Scholar
- Väisänen-Rhen V: Fimbria-like hemagglutinin of Escherichia coli O75 strains. Infect Immun. 1984, 46: 401-407.PubMedPubMed CentralGoogle Scholar
- Lublin DM, Coyne KE: Phospholipid-anchored and transmembrane version of either decay-avvelerating factor or membrane cofactor protein show equal efficiency in protection from complement-mediated cell damage. J Exp Med. 1991, 174: 35-10.1084/jem.174.1.35.PubMedView ArticleGoogle Scholar
- Aberg V, Sellstedt M, Hedenström M, Pinkner JS, Hultgren SJ, Almqvist F: Design, synthesis and evaluation of peptidomimetics based on substituted bicyclic 2-pyridones-targeting virulence of uropathogenic E. coli. Bioorg Med Chem. 2006, 14: 7563-7581. 10.1016/j.bmc.2006.07.017.View ArticleGoogle Scholar
- Aberg V, Das P, Chorell E, Hedenström M, Pinkner JS, Hultgren SJ, Almqvist F: Carboxylic acid isosteres improve the activity of ring-fused 2-pyridones that inhibit pilus biogenesis in E. coli. Bioorg Med Chem Lett. 2008, 18: 3536-3540. 10.1016/j.bmcl.2008.05.020.PubMed CentralView ArticleGoogle Scholar
- Aberg V, Fällman E, Axner O, Uhlin BE, Hultgren SJ, Almqvist F: Pilicides regulate pili expression in E. coli without affecting the functional properties of the pilus rod. Mol Biosyst. 2007, 3: 214-218. 10.1039/b613441f.PubMedView ArticleGoogle Scholar
- Pettigrew D, Anderson KL, Billington J, Cota E, Simpson P, Urvil P, Rabuzin F, Roversi P, Nowicki B, du Merle L, et al: High resolution studies of the Afa/Dr adhesin DraE and its interaction with chloramphenicol. J Biol Chem. 2004, 279: 46851-46867. 10.1074/jbc.M409284200.PubMedView ArticleGoogle Scholar
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