Figure 2

Blocking of Dr fimbriae-dependent agglutination of human erythrocytes by pilicides. The following bacterial preparations, normalized to OD₆₀₀, were used in the hemagglutination assays: negative control – E. coli BL21DE3/pACYC184, grown on TSA plates with 5% DMSO, non-fimbriated strain; positive control - E. coli BL21DE3/pBJN406, grown on TSA plates without pilicide, fully-fimbriated strain; chloramphenicol –E. coli BL21DE3/pBJN406, grown on TSA plates without pilicide, the agglutination experiment was performed in the presence of 2 μM of chloramphenicol; pilicide 1 and pilicide 2 - the E. coli BL21DE3/pBJN406 grown on the TSA plates in the presence of 3.5 mM of agents 1 and 2, respectively. HA titer represents two-fold serial dilutions of normalized bacterial suspensions. The initial 1, 2 and final 128 dilutions are not presented. In the case of HA assays with bacteria cultivated in media in the presence of pilicide the black triangles mark the highest dilution which still provides visible agglutination.