Figure 1

Blocking the adherence of E. coli Dr⁺strain to CHO-DAF⁺cells by pilicides. The propensity of bacteria binding to CHO-DAF⁺ and CHO-DAF^- cells was evaluated by staining with Giemsa (magnification x 10 000, Olympus CKX41 microscope). The following bacterial preparations were used in the adherence assays: negative control – E. coli BL21DE3/pACYC184, grown on TSA plates with 5 % DMSO, non-fimbriated strain; positive control - E. coli BL21DE3/pBJN406, grown on TSA plates without pilicide, fully-fimbriated strain; pilicide 1 and pilicide 2 - E. coli BL21DE3/pBJN406 grown on TSA plates in the presence of 0.5, 1.5, 2.5 and 3.5 mM of agents 1 and 2, respectively. In the experiments presented by M and N panels the E. coli BL21DE3/pBJN406 strain was grown in the presence of 3.5 mM of pilicides. The figure presents representative results obtained from three independent experiments. Each experiment was composed from the four-fold repetition for each used bacterial preparation. The bacterial adherence to 40 CHO cells was determined for each repetition. Presented in the figure pilicides 1 and 2 are the literature agents which, at a 3.5 mM concentration, inhibit the assembly of FGS type 1 and P pili.