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Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita

BMC Microbiology volume 20, Article number: 299 (2020) Cite this article

Abstract

Background

Root-knot nematode is one of the most significant diseases of vegetable crops in the world. Biological control with microbial antagonists has been emerged as a promising and eco-friendly treatment to control pathogens. The aim of this study was to screen and identify novel biocontrol agents against root-knot nematode, Meloidogyne incognita.

Results

A total of 890 fungal isolates were obtained from rhizosphere soil of different crops and screened by nematicidal activity assays. Snef1910 strain showed high virulence against second stage juveniles (J2s) of M. incognita and identified as Trichoderma citrinoviride by morphology analysis and biomolecular assay. Furthermore, T. citrinoviride Snef1910 significantly inhibited egg hatching with the hatching inhibition percentages of 90.27, 77.50, and 67.06% at 48, 72, and 96 h after the treatment, respectively. The results of pot experiment showed that the metabolites of T. citrinoviride Snef1910 significantly decreased the number of root galls, J2s, and nematode egg masses and J2s population density in soil and significantly promoted the growth of tomato plants. In the field experiment, the biocontrol application showed that the control efficacy of T. citrinoviride Snef1910 against root-knot nematode was more than 50%. Meanwhile, T. citrinoviride Snef1910 increased the tomato plant biomass.

Conclusions

T. citrinoviride strain Snef1910 could be used as a potential biological control agent against root-knot nematode, M. incognita.

Background

Root-knot nematode (RKN) is one of the most seriously damaging plant-parasitic nematode in the world threatening to the growth and production of more than 5500 plants, including vegetable crops and weeds [1,2,3]. Among them, Meloidogyne incognita, M. arenaria, M. javanica, and M. hapla are the four main root-knot nematodes that have been reported. M. incognita is the most serious with 51% of the affected crops and the highest distribution proportion among these four species [4]. With rapidly increased developing protected agriculture, high-value crops, such as tomato (Solanum lycopersicum cultivar L-402 susceptible to M. incognita), are severely damaged by M. incognita and has led to severe losses in China [5]. Currently, chemical and physical measures have limited use for RKN disease management. Furthermore, as the nematicides are associated with serious environmental problems. Therefore, the safe and effective strategies for management for M. incognita are needed.

Biological control with microbial antagonists has received a great deal of attention as a promising measure to control different plant diseases [6, 7]. Many antagonistic microorganisms including Trichoderma spp., Streptomyces spp., Pseudomonas spp., Bacillus spp. have been screened and widely exploited to control a wide range of plant pathogens [8,9,10,11]. Some microorganisms have been identified as biological control agents against M. incognita such as Pasteuria penetrans, B. subtilis, T. harzianum and T. viride, Pochonia chlamydosporia and Purpureocillium lilacinum [12,13,14,15,16]. Trichoderma genus is one of the most frequently studied groups of fungi used as biological control agents. Its species are often very fast growing, rapidly colonize substrates and effectively control different diseases by using a variety of mechanisms [17, 18]. At present, there are no reports about using T. citrinoviride as a biological control agent against RKN, M. incognita.

The objective of this study was to isolate effective fungal strains against M. incognita, evaluate the biological control activity under in vitro and in vivo, phylogenetically identify them based on morphology and sequence analysis and their effects on the growth of tomato plants in pot and field experiments.

Results

Screening of antagonistic fungi

In total, 890 fungi isolates were obtained from rhizosphere soil of different crops and screened for the potential nematicidal action against M. incognita in vitro (Table S1). Among these isolates, strain Snef1910 (CGMCC Accession No.13569; China General Microbiology Culture Collection Center) showed the strongest nematicidal activity against second stage juveniles (J2s) of M. incognita. The percentages of J2s mortality of strain Snef1910 were 93.79, 98.20, and 100% at 24 h, 48 h, and 72 h, respectively (Fig. 1). Moreover, strain Snef1910 showed significant antagonistic activity in vitro towards other pathogens that caused plant diseases in wheat, cotton, melon and other plants (Table 1).

Fig. 1
figure 1

The effect of strain Snef1910 on second stage juvenile (J2s) mortality of M. incognita in vitro. The J2s suspension mixed with PDB medium alone was used as control (CK). Error bars represent standard deviation. ∗The data on the columns represent the corrected J2s mortality. J2s mortality (%) = the number of dead J2s/total number of J2s × 100. Corrected J2s mortality (%) = (J2s mortality in the treatment – J2s mortality in the control)/(100 – J2s mortality in the control) × 100

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Table 1 Inhibitory activity of strain Snef1910 against various fungal plant pathogens in vitro
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Evaluation of ovicidal efficacy in vitro

To further investigate the nematicidal activity of strain Snef1910, the ovicidal efficacy against M. incognita was performed. The egg hatching percentage of M. incognita treated with PDB medium (control) was 51.17% at 48 h, while in treatment with strain Snef1910 culture filtrate it was 4.98% (Fig. 2). The hatch inhibition percentage of strain Snef1910 was up to 90.27% (Fig. 2). Similar results were obtained at 72 h and 96 h (Fig. 2).

Fig. 2
figure 2

The egg inhibition of M. incognita exposed to strain Snef1910 culture filtrate in vitro. CK denotes for the PDB medium. ∗The data on the columns represent the hatch inhibition percentage. Egg hatching percentage (%) = the number of hatched eggs/total number of eggs × 100. Hatch inhibition percentage (%) = (the number of hatched eggs in the control – the number of hatched eggs in the fungus-treated group)/the number of hatched eggs in the control × 100

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Identification of strain Snef1910

To identify strain Snef1910, morphological observation was performed. The strain Snef1910 grew rapidly on PDA medium forming a white, 60-mm-diameter colony at 25 °C under dark for 2 days. Then, the colony changed to greyish green or dark green and formed a wide conidial zone at the edge of the colony at 7 days (Fig. 3a-c). The conidiophores were erect showing a long axis of the structure and fertile to the top. The cylindrical or spindle-shaped bottle stems were solitary born on main branch and spirally arranged on top of lateral branches (Fig. 3d). The conidia were yellowish green to dark green, globose with smooth walls (Fig. 3e). No distinct coconut-like odor was detected. Based on the above microscopic observations, strain Snef1910 was tentatively identified as Trichoderma.

Fig. 3
figure 3

Colony characteristics and conidiophores and conidia of strain Snef1910. a-c Colony appearance of strain Snef1910 grown on PDA at 25 °C for 2–7 days. d Microscopic image showing conidiophores structures and branching pattern of strain Snef1910. e Image of conidia of strain Snef1910

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To further identify strain Snef1910, a phylogenetic analysis of its 5.8S-ITS sequences was performed. The phylogenetic tree showed that the 5.8S-ITS sequence (GenBank accession number: YK964310) of strain Snef1910 was clustered with T. citrinoviride (Fig. 4). These results demonstrated that strain Snef1910 was identified as T. citrinoviride.

Fig. 4
figure 4

Phylogenetic tree of strain Snef1910 based on the partial nucleotide sequences of 5.8S-ITS. A neighbor-joining phylogenetic tree of strain Snef1910 was constructed using MEGA 4.0. The percentage numbers at the nodes indicate the levels of bootstrap support based on a neighbor-joining analysis of 1000 resampled datasets; only values greater than 50% are provided. The scale bar indicates 0.020 nucleotide substitutions per nucleotide position

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Biocontrol of root-knot nematode in the pot experiment

In order to investigate the potential biological control activity of T. citrinoviride Snef1910 against M. incognita in vivo, tomato seedlings were treated with a fermentation broth of T. citrinoviride and inoculated with J2s of M. incognita. The number of root galls on tomato seedlings treated with PDB culture medium (PDB culture medium-inoculated) was 47.35, thirty days post-inoculation of M. incognita, while the number of root galls on tomato seedlings treated with fermentation broth of T. citrinoviride Snef1910 and untreated were 16.05 and 48.15, respectively (Table 2). The number of root galls on tomato seedlings treated with T. citrinoviride Snef1910 was reduced by 66.10% compared with that of the PDB culture medium-inoculated (Table 2). T. citrinoviride Snef1910 caused significant decreases in the number of egg masses and J2s on tomato seedling root, which were 80.63 and 69.87% reduced, respectively, compared with those in the control (PDB culture medium-inoculated) (Table 2). Furthermore, thirty days after M. incognita inoculation, the number of J2s per 100 mL soil of T. citrinoviride Snef1910-treated seedlings was significantly smaller than that of the control (PDB culture medium-inoculated), with mortality reaching 77.28% (Table 2). Thirty days after treatment, T. citrinoviride Snef1910-treated plants exhibited increases in shoot length, root length, root fresh weight, and root dry weight by 15.61, 23.32, 35.08, and 33.33%, respectively, compared with untreated plants (Table 3).

Table 2 The biological control activity of T. citrinoviride Snef1910 against M. incognita in the pot experiment with tomato plants
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Table 3 The effects of T. citrinoviride Snef1910 on the growth parameters of tomato plants in pot experiments
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Biocontrol of root-knot nematode in the field experiment

The field experiments showed similar results to the greenhouse pot experiments. At 30 days after transplantation, the gall index of seedlings treated with T. citrinoviride Snef1910 were 42.67 and 37.78 while those treated with PDB (control) were 86.67 and 80.00 (Table 4) which indicated a noticeable reduction of RKN during a two-growth seasons of field experiment. Furthermore, the biocontrol efficacy of T. citrinoviride Snef1910 in controlling M. incognita reached up to 50.77 and 52.77% in the field experiments (Table 4; Fig. 5). These results indicated that T. citrinoviride Snef1910 is a biological control agent for efficiently controlling RKN. Shoot length, root length, fresh weight of root were also measured in the field experiments. The growth-promoting results in field experiments were similar to the greenhouse pot experiments. The shoot length and root length in T. citrinoviride Snef1910 treated plants increased by 13.48 and 17.98%, respectively, in comparison with the control plants (Table 5), which suggested that T. citrinoviride Snef1910 promotes the growth of tomato. In contrast, T. citrinoviride Snef1910 showed a 35.62% reduction in the fresh weight of root, thirty days after transplantation (Table 5). Similar results were obtained in the second field experiment (November to December in 2019) (Table 5). These results suggested that T. citrinoviride Snef1910 has the potential in increasing tomato plant growth.

Table 4 The biocontrol effect of T. citrinoviride Snef1910 against M. incognita in the field experiment
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Fig. 5
figure 5

Biocontrol of T. citrinoviride Snef1910 against M. incognita in the field experiment from November to December in 2019. a The M. incognita infected tomato plant roots, b the biocontrol effect of T. citrinoviride Snef1910 on tomato plant roots. Root gall indices were rated using a scale of 0–5, where 0, no gall; 1,15% or less roots with galls; 2–4, 16–25%; 26–50%; 51–75% roots with galls, respectively; and 5,> 76% roots with galls. Gall index = ∑the number of diseased plants in each grade × grade/(total number of plants investigated × the highest grade) × 100%. Biocontrol efficacy (%) = (gall index in the control − gall index in the Snef1910-treated group)/ gall index in the control × 100

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Table 5 The effect of T. citrinoviride Snef1910 on the growth parameters of tomato plants infected with M. incognita in the field experiments
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Discussion

Researches on the use of antagonistic microorganisms to suppress plant diseases are receiving increasingly attention [19]. In recent years, Trichoderma spp. has been used as biocontrol agents to control different plant pathogens [20, 21]. Specifically, previous studies have reported that T. viride MTCC No. 167, T. asperellum T-12, and T. harzianum T-78 were potential biological agents against M. incognita [22,23,24,25]. Furthermore, a few other microorganisms such as S. rubrogriseus, B. subtilis, P. putida and P. fluorescens have been employed in controlling M. incognita [13, 26]. However, the microorganism sources used for the control of M. incognita are limited and need further explored. In this study, T. citrinoviride Snef1910 strain was screened from 890 fungal isolates and showed high larvicidal and ovicidal activity against M. incognita in vitro. Furthermore, T. citrinoviride Snef1910 strain significantly reduced the galls and J2s of nematodes, and promoted the growth of tomato plant in the pot and field trials. This study is the first report on the use of a T. citrinoviride strain as a potential biological control agent to control RKN disease. The results of our study provide an alternative and promising biological control agent and practical strategies for controlling RKN, M. incognita.

Trichoderma species have been reported as biological control agents in controlling RKN disease [23,24,25]. For example, nematode galling caused by M. incognita on tomato showed reductions of up to 30.8% under the soil treatments with T. harzianum and T. viride [27]. Meanwhile, T. harzianum can reduced galling caused by another RKN M. javanica on tomato plants [28]. In addition, the biocontrol efficacy of another species T. longibrachiatum in controlling M. incognita was indicated from 30 to 78% for gall index on cucumber [29]. In the present study, the damage of RKN can reduce over 50%, which suggested that T. citrinoviride Snef1910 has more biocontrol efficiency and potential application to control RKN M. incognita on tomato.

Multiple action modes of Trichoderma species were reported to contribute to the biological control, including nutrient and space competition, antibiosis, mycoparasitism, and induced systemic resistance of plants [30,31,32,33]. Particularly, the antimicrobial compounds produced by Trichoderma species and their roles in controlling plant pathogens have been characterized [34,35,36]. For example, acetic acid isolated from culture filtrates of T. longibrachiatum showed nematicidal activity against Meloidogyne spp. [37]. Many biocontrol activators obtained from Trichoderma spp. have been proven to be nematicidal compounds such as trichodermin and trypsin-like protease [38,39,40,41]. Our results indicated that cultural filtrates of T. citrinoviride Snef1910 strain showed high ovicidal and larvicidal activities (Fig. 1, 2). Further research will be conducted to identify and characterize the nematicidal compounds of T. citrinoviride Snef1910 against M. incognita.

The effective ways of biocontrol products and microorganism application should be applied according to the characteristics of the biocontrol agents and the routine practices of agricultural producers [42]. For example, the soil treatment with the culture broth of B. subtilis isolate B10 was highly significant in decreasing number of galls and egg masses of M. incognita with reduction percentage of 81.1 and 89.5%, respectively [43]. Application of the fermentation broth of five bacterial strains by coating tomato seeds showed high biocontrol efficacy against M. incognita [26]. Moreover, soil treatment with the biocontrol agents T. harzianum and T. viride improved the efficiency of nematode control by reductions of up to 30.8% in nematode galling on tomato [27, 44]. Na et al. [5] reported that drenching of the broth containing spores was the appropriate application method for S. rubrogriseus HDZ-9-47 to control M. incognita, which reduced the root knot index and J2s density by 51.1 and 80.7%, respectively. In this study, T. citrinoviride Snef1910 significantly reduced the galls and nematodes in the pot and field trials through application the fermentation broth (Tables 2, 4). Therefore, drenching the fermentation broth is one of the application approaches for T. citrinoviride Snef1910 to control RKN. Meanwhile, other application such as seed coating of T. citrinoviride Snef1910 will be expected to study in the future.

Moreover, the plant growth promotion is well characterized in Trichoderma species. For example, T. harzianum T969 increased the shoot height (58.70%), shoot diameter (58.03%), root fresh weight (78.92%) and dry weight (93.07%) of tomato seedlings [45]. The mechanisms of Trichoderma in promoting plant growth also involve the production of auxin-like compounds, improving availability of nutrients, affecting root system, and inducing of systemic resistance phenomenon [46,47,48,49]. For example, the releasing an auxin-like phytohormone of T. harzianum SQR-T037 significantly promoted tomato seedling growth by up to 2.5-fold dry weight [46]. In this study, T. citrinoviride Snef1910 resulted in increased plant growth of tomato in the pot and field experiments (Tables 3, 5). More work is also planned to explore the growth promotion effects and mechanisms of T. citrinoviride Snef1910 in tomato and even other plants.

Conclusions

In conclusion, this study showed that T. citrinoviride Snef1910 was screened from 890 fungi isolates and efficiently controlled RKN disease caused by M. incognita, which played control efficacy of more than 50% and increased egg hatching inhibition percentages and reduced root galls, egg masses and J2s on tomato. Moreover, T. citrinoviride Snef1910 showed the plant growth promotion of shoot and root length of tomato. This is the first report on the use of a T. citrinoviride strain as a potential biological control source to control RKN disease caused by M. incognita. This study provides a new biological control agent and potentially practical strategies for sustainable management of RKN.

Methods

Isolation of fungal strains

Fungal strains used in this study were originally isolated from rhizosphere soil collected from health plants including tomatoes, cucumbers, soybean, eggplant, peanut, and corn in different locations in northeast China. For fungal isolation, the soil serial dilution plate method was used [50]. In brief, a 1 g of rhizosphere soil samples was added into 9 mL of sterilized water and then mixed to obtain soil suspension. The soil suspension was serially diluted to the appropriate concentration (10− 2 g/mL and 10− 3 g/mL) and plated on Potato Dextrose Agar (PDA) plates. The plates were then incubated at 28 °C for 5 days. Individual fungal colonies were isolated and purified.

Preparation of nematode inoculum

The root-knot nematode M. incognita, which sampled from the invasive tomato field located in Tieling County (123.92E, 42.18 N), Liaoning Province, China, was maintained on tomato plants in the greenhouse of Nematology Institute of Northern, Shenyang Agricultural University, China. Eggs were separated from masses that collected from the roots of tomato plants according to the method of Martinuz et al. [51]. The J2s were obtained by incubating the eggs in sterile water at 25 °C for 5 days and collected every 24 h. Then, the J2s were diluted to the appropriate concentration and used for further study.

Screening of fungal isolates against M. incognita in vitro

The J2s of M. incognita were used to detect the nematicidal activities of fungal isolates. The fermentation broth of fungal strains was prepared in Potato Dextrose Broth (PDB) medium in a shaker at 150 rpm for 5 days at 28 °C. Cell-free supernatant was obtained after centrifugation at 4500×g for 15 min and filtered using the filter (φ = 0.45 μm). A 50 μL suspension containing 50 J2s was added into each petri dish with 950 μL of the prepared fungal cell-free supernatant. The J2s suspension mixed with PDB medium alone was used as control. Five dishes were used for each treatment. All the dishes were incubated at 25 °C for 72 h. The number of dead and alive J2s was examined using the stereoscopic microscope at 24 h, 48 h, and 72 h after treatment. A nematode that malformed, immobile or motionless even probed with a fine needle was deemed dead [52]. The J2s mortality and corrected J2s mortality were calculated as following:

$$ \mathrm{J}2\mathrm{s}\ \mathrm{mortality}\ \left(\%\right)=\mathrm{the}\ \mathrm{number}\ \mathrm{of}\ \mathrm{dead}\ \mathrm{J}2\mathrm{s}/\mathrm{total}\ \mathrm{number}\ \mathrm{of}\ \mathrm{J}2\mathrm{s}\kern0.5em \times 100. $$
$$ \mathrm{Corrected}\ \mathrm{J}2\mathrm{s}\ \mathrm{mortality}\ \left(\%\right)=\left(\mathrm{J}2\mathrm{s}\ \mathrm{mortality}\ \mathrm{in}\ \mathrm{the}\ \mathrm{treatment}\hbox{-} \mathrm{J}2\mathrm{s}\kern0.2em \mathrm{mortality}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}\right)/\left(100\hbox{-} \mathrm{J}2\mathrm{s}\ \mathrm{mortality}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}\right)\times 100 $$

The strains that exhibited the strongest nematicidal activity against J2s of M. incognita were chosen for the future study. The effects of selected strains on J2s mortality of M. incognita were conducted as described above and repeated three times.

In vitro antagonism test

The dual culture technique was used to detect the antagonistic activities of strain Snef1910 against fungal plant pathogens (Fusarium graminearum, F. oxysporum, F. moniliforme, F. roseum, Gaeumannomyces graminis, Rhizoctonia cerealis, Verticillium alboatrum) [53]. In brief, the pathogen discs (5 mm diameter) were placed on one side of PDA plates (90 mm) around the center at a distance of 3 cm. The plugs of strain Snef1910 was placed on the opposite side and around the fungal inocula at a distance of 6 cm. PDA plates inoculated with the pathogen alone were used as a control. The plates were incubated at 28 °C for 5 days. The antagonistic activity of strain Snef1910 was assessed by measuring the colony diameters. The percentage of growth inhibition was calculated using the formula R = (a–b)/a × 100, where R, a, and b is the percentage of growth inhibition, the mycelial radial growth of the pathogen in the control and in the presence of the antagonist, respectively. The values were recorded as the means of three replicates, and the experiments were repeated three times. The 7 plant pathogens tested in this study were kindly provided by the College of Plant Protection, China Agricultural University, China.

Testing the ovicidal efficacy of strain Snef1910 against M. incognita in vitro

The ovicidal efficacy of strain Snef1910 against M. incognita was performed as previously described [26]. In short, 200 eggs were carefully added to 500 μL of strain Snef1910 metabolites in each well of 96-well microtiter plates. Eggs transferred into PDB medium alone were used as controls. The plates were incubated at 28 °C for 96 h. In order to stop further hatching, 500 μL of Lugol’s iodine solution was added to each well [25]. The numbers of the unhatched eggs were counted under microscope [5], which were used to calculate the hatching percentage, hatch inhibition and corrected egg inhibition by the following formula:

$$ \mathrm{Egg}\ \mathrm{hatching}\ \mathrm{percentage}\ \left(\%\right)=\mathrm{the}\ \mathrm{number}\ \mathrm{of}\ \mathrm{hatched}\ \mathrm{eggs}/\mathrm{total}\ \mathrm{number}\ \mathrm{of}\ \mathrm{eggs}\times 100. $$
$$ \mathrm{Hatch}\ \mathrm{in}\mathrm{hibition}\ \left(\%\right)=\left(\mathrm{the}\ \mathrm{number}\ \mathrm{of}\ \mathrm{hatched}\ \mathrm{eggs}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}-\mathrm{the}\ \mathrm{number}\ \mathrm{of}\ \mathrm{hatched}\ \mathrm{eggs}\ \mathrm{in}\ \mathrm{the}\ \mathrm{fungus}-\mathrm{treated}\ \mathrm{group}\right)/\mathrm{the}\ \mathrm{number}\ \mathrm{of}\ \mathrm{hatched}\ \mathrm{eggs}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}\times 100. $$
$$ \mathrm{Corrected}\ \mathrm{egg}\ \mathrm{inhibition}\ \left(\%\right)=\left(\mathrm{The}\ \mathrm{hatch}\ \mathrm{in}\mathrm{hibition}\ \mathrm{in}\ \mathrm{the}\ \mathrm{treatment}-\mathrm{the}\ \mathrm{hatch}\mathrm{ed}\ \mathrm{in}\mathrm{hibition}\ \mathrm{in}\ \mathrm{control}\right)/\left(100-\mathrm{hatch}\ \mathrm{in}\mathrm{hibition}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}\right) $$

Three wells were used for each replicate, and the values were recorded as the means of three replicates for each treatment. Ovicidal experiments were repeated three times.

Identification of strain Snef1910

To identify strain Snef1910, the growth pattern and microscopic observation of the morphology of conidia and conidiophores were performed. Single spore of strain Snef1910 was grown on the PDA plate at 25 °C for 5 days. Then, a 5-mm-diameter fungal plug was placed in the center of PDA plates (90 mm) at 25 °C in the dark for 7 days. The color, smell, growth, and shape of the colony and conidiophores branching pattern and conidia were examined [54, 55].

Afterwards, strain Snef1910 was further identified through a phylogenetic analysis of its 5.8S-ITS region sequence. Genomic DNA of Snef1910 strain was extracted using a N96 Plant Genomic DNA Kit (Tiangen Biotech Co., Ltd., Beijing, China) according to the manufacturer’s instructions. The fragment of 5.8S-ITS region was amplified with primer pair ITS1 and ITS4 [56]. The PCR product was ligated into the pMD19-T vector (Takara Co., Ltd., Dalian, China), and the resulting recombinant plasmid was sent to Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China) for sequencing. The phylogenetic tree of strain Snef1910 based on the sequence of 5.8S-ITS region was constructed using the neighbor-joining method in MEGA 4.0 software [57]. The topology of the phylogenetic tree was evaluated using1000 bootstrap resampling replicates.

Biocontrol of root-knot nematode in the pot experiments

The tomato (Solanum lycopersicum cultivar L-402 susceptible to M. incognita) was used to evaluate the biocontrol potentials of strain Snef1910. Tomato seeds were surface sterilized with 1% (v/v) sodium hypochlorite solution for 5 min, followed by 3 times washings with sterile distilled water [58]. Three seeds were sown in plastic pots (5 × 10 holes) filled with 42 g of soil, vermiculite and sand at a ratio of 2:1:1. The sand and soils were autoclaved at 165 °C for 120 min before planting. During the entire course of the experiment, the plants were watered two times and fertilized Hoagland solution once a week [24]. After attaining 2-leaf stage, the tomato seedlings were transplanted into 13 cm × 12 cm plastic pots containing 600 g of autoclaved soil, sand and vermiculite at a ratio of 2:1:1 (one tomato seedling per pot). The pots were placed in a greenhouse under the following conditions: 28 ± 2 °C, 60% humidity, and 16 h of light alternating with 8 h of darkness.

The fermentation broth (2.15 × 106 spores/mL) of strain Snef1910 was prepared by the method described above. After transplanting for 2 days, each pot was treated with 2 mL fermentation broth of strain Snef1910. At the same time, each seedling was inoculated with 5 mL of M. incognita suspension containing 2000 motile J2s, and named Snef1910-inoculated. In addition, the following four treatments were maintained as controls: untreated + uninoculated control (untreated-uninoculated), untreated-uninoculated + inoculated M. incognita (untreated-inoculated), 2 mL of fermentation broth of strain Snef1910-uninoculated (Snef1910-uninoculated), and 2 mL PDB culture medium-inoculated (PDB culture medium-inoculated). The fermentation broth of strain Snef1910 and PDB culture medium filtrate in treatments of PDB culture medium-inoculated, Snef1910-uninoculated, and Snef1910-inoculated were suspended in 100 mL of sterile distilled water and applied by drenching roots. Each treatment included 10 replicates, and each replicate included one tomato plant. The experimental design for pot assay was performed a randomized complete block design.

Thirty days after inoculation, the tomato plants with their rhizosphere soil samples were collected. To evaluate the biocontrol potentials of strain Snef1910, shoot length, root length, fresh and dry weights of root, the number of root galls, J2s and egg masses per 1 g root, and J2s per 100 mL soil were counted as previously described [26, 59]. The experiments were repeated three times.

Biocontrol of root-knot nematode in the field experiment

The field experiment was carried out in a field naturally and severely infested with M. incognita, located in Tieling County, Liaoning Province, China (123.92E, 42.18 N) in two growing seasons (from August to September and November to December) in 2019 with the temperature ranged from 15 °C to 35 °C. The soil in protected field was determined as a brown soil with the properties of pH 5.56 ± 0.08, organic matter 70.76 g /kg, and total nitrogen, available potassium, and available phosphorus of 4.56, 285.95, and 81.68 mg/kg, respectively. The four-week-old tomato seedlings without infestation with M. incognita were transplanted into the field. The 196 mL sterile distilled water was added into 4 mL fermentation broth (106 spores/mL) of strain Snef1910 or 4 mL PDB culture medium to obtain the desired concentrations and poured into the planting hole during plant transplantation. The experimental plots were 6 m long, 5.5 m wide and separated by 0.5 m (6 plant rows) and contained 21 transplanted seedlings per row. A randomized complete block design was adopted in this experiment, and each treatment consisted of three replications. The protected fields were irrigated and fertilized followed by farming practice.

Fifteen plants and rhizosphere soil samples were randomly selected and collected form each treatment, 30 days after transplantation. The shoot length, root length, and fresh weight of root were measured as described above. Root gall indices were rated using a scale of 0–5, where 0, no gall; 1,15% or less roots with galls; 2–4, 16–25%; 26–50%; 51–75% roots with galls, respectively; and 5,> 76% roots with galls [60]. The root gall index and biocontrol efficacy were calculated as follow:

$$ \mathrm{Gall}\ \mathrm{in}\mathrm{dex}=\sum \mathrm{the}\ \mathrm{number}\ \mathrm{of}\ \mathrm{diseased}\ \mathrm{plants}\ \mathrm{in}\ \mathrm{each}\ \mathrm{grade}\times \mathrm{grade}/\left(\mathrm{total}\ \mathrm{number}\ \mathrm{of}\ \mathrm{plants}\ \mathrm{in}\mathrm{vestigated}\times \mathrm{the}\ \mathrm{highest}\ \mathrm{grade}\right)\times 100\%. $$
$$ \mathrm{Biocontrol}\ \mathrm{efficacy}\ \left(\%\right)=\left(\mathrm{gall}\ \mathrm{in}\mathrm{dex}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}-\mathrm{gall}\ \mathrm{in}\mathrm{dex}\ \mathrm{in}\ \mathrm{the}\ \mathrm{Snef}1910-\mathrm{treated}\ \mathrm{group}\right)/\mathrm{gall}\ \mathrm{in}\mathrm{dex}\ \mathrm{in}\ \mathrm{the}\ \mathrm{control}\times 100. $$

Statistical analysis

Data were statistically analyzed using SPSS software 20.0. Duncan’s one-way analysis of variance was used to determine the significant differences.

Availability of data and materials

The datasets used and analysed during the current study are available from the corresponding author on reasonable request. All data generated or analysed during this study are included in this article.

Abbreviations

J2s:

Second stage juveniles

PCR:

Polymerase Chain Reaction

PDA:

Potato Dextrose Agar

PDB:

Potato Dextrose Broth

RKN:

Root-knot nematode

References

  1. Jones JT, Haegeman A, Danchin EG, Gaur HS, Helder J, Jones MG, et al. Top 10 plant-parasitic nematodes in molecular plant pathology. Mol Plant Pathol. 2013;14(9):946–61.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Trudgill DL, Blok VC. Apomictic, polyphagous root-knot nematodes: exceptionally successful and damaging biotrophic root pathogens. Ann Rev Phytopathol. 2001;39(1):53–77.

    Article  CAS  Google Scholar 

  3. d’Errico G, Crescenzi A, Landi S. First report of the southern root-knot nematode Meloidogyne incognita on the invasive weed Araujia sericifera in Italy. Plant Dis. 2014;98(11):1593–4.

    Article  PubMed  Google Scholar 

  4. Castagnone-Sereno P, Danchin EG, Perfus-Barbeoch L, Abad P. Diversity and evolution of root-knot nematodes, genus Meloidogyne: new insights from the genomic era. Ann Rev Phytopathol. 2013;51:203–20.

    Article  CAS  Google Scholar 

  5. Na J, Hui X, LI W-J, WANG X-Y, Qian L, LIU S-S, Pei L, J-l ZHAO, Heng J. Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato. J Integr Agric. 2017;16(6):1347–57.

    Article  Google Scholar 

  6. Sharifazizi M, Harighi B, Sadeghi A. Evaluation of biological control of Erwinia amylovora, causal agent of fire blight disease of pear by antagonistic bacteria. Biol Control. 2017;104:28–34.

    Article  Google Scholar 

  7. Vega FE. The use of fungal entomopathogens as endophytes in biological control: a review. Mycologia. 2018;110(1):4–30.

    Article  PubMed  Google Scholar 

  8. Bunbury-Blanchette AL, Walker AK. Trichoderma species show biocontrol potential in dual culture and greenhouse bioassays against Fusarium basal rot of onion. Biol Control. 2019;130:127–35.

    Article  Google Scholar 

  9. Sarwar A, Latif Z, Zhang S, Zhu J, Zechel D, Bechthold A. Biological control of potato common scab with rare isatropolone C compound produced by plant growth promoting Streptomyces A1RT. Front Microbiol. 2018;9:1126.

    Article  PubMed  PubMed Central  Google Scholar 

  10. d’Errico G, Marra R, Crescenzi A, Davino SW, Fanigliulo A, Woo SL, Lorito M. Integrated management strategies of Meloidogyne incognita and Pseudopyrenochaeta lycopersici on tomato using a Bacillus firmus-based product and two synthetic nematicides in two consecutive crop cycles in greenhouse. Crop Prot. 2019;122:159–64.

    Article  Google Scholar 

  11. Aiello D, Restuccia C, Stefani E, Vitale A, Cirvilleri G. Postharvest biocontrol ability of Pseudomonas synxantha against Monilinia fructicola and Monilinia fructigena on stone fruit. Postharvest Biol Technol. 2019;149:83–9.

    Article  Google Scholar 

  12. Bhuiyan S, Garlick K, Anderson J, Wickramasinghe P, Stirling G. Biological control of root-knot nematode on sugarcane in soil naturally or artificially infested with Pasteuria penetrans. Australas Plant Pathol. 2018;47(1):45–52.

    Article  CAS  Google Scholar 

  13. Rao M, Kamalnath M, Umamaheswari R, Rajinikanth R, Prabu P, Priti K, Grace G, Chaya M, Gopalakrishnan C. Bacillus subtilis IIHR BS-2 enriched vermicompost controls root knot nematode and soft rot disease complex in carrot. Sci Horticulturae. 2017;218:56–62.

    Article  Google Scholar 

  14. Mukhtar T. Management of root-knot nematode, Meloidogyne incognita, in tomato with two Trichoderma species. Pak J Zool. 2018;50(4):1589–92.

    Google Scholar 

  15. Ghahremani Z, Escudero N, Saus E, Gabaldon T, Javier Sorribas F. Pochonia chlamydosporia induces plant-dependent systemic resistance to Meloidogyne incognita. Front Plant Sci. 2019;10:945.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Huang W-K, Cui J-K, Liu S-M, Kong L-A, Wu Q-S, Peng H, He W-T, Sun J-H, Peng D-L. Testing various biocontrol agents against the root-knot nematode (Meloidogyne incognita) in cucumber plants identifies a combination of Syncephalastrum racemosum and Paecilomyces lilacinus as being most effective. Biol Control. 2016;92:31–7.

    Article  Google Scholar 

  17. Vinale F, Sivasithamparam K, Ghisalberti EL, Marra R, Woo SL, Lorito M. Trichoderma-plant-pathogen interactions. Soil Biol Biochem. 2008;40(1):1–10.

    Article  CAS  Google Scholar 

  18. Ozbay N, Newman SE. Biological control with Trichoderma spp. with emphasis on T. harzianum. Pak J Biol Sci. 2004;7(4):478–84.

    Article  Google Scholar 

  19. Dukare AS, Paul S, Nambi VE, Gupta RK, Singh R, Sharma K, Vishwakarma RK. Exploitation of microbial antagonists for the control of postharvest diseases of fruits: a review. Crit Rev Food Sci Nutr. 2019;59(9):1498–513.

    Article  CAS  PubMed  Google Scholar 

  20. Bae S-J, Mohanta TK, Chung JY, Ryu M, Park G, Shim S, et al. Trichoderma metabolites as biological control agents against Phytophthora pathogens. Biol Control. 2016;92:128–38.

    Article  CAS  Google Scholar 

  21. Giurgiu RM, Dumitraș A, Morar G, Scheewe P, Schroeder FG. A study on the biological control of Fusarium oxysporum using Trichoderma spp., on soil and rockwool substrates in controlled environment. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2018;46(1):260–9.

    Article  CAS  Google Scholar 

  22. Sonkar SS, Bhatt J, Meher J, Kashyap P. Bio-efficacy of Trichoderma viride against the root-knot nematode (Meloidogyne incognita) in tomato plant. J Pharmacognosy Phytochemistry. 2018;7(6):2010–4.

    CAS  Google Scholar 

  23. Affokpon A, Coyne DL, Htay CC, Agbèdè RD, Lawouin L, Coosemans J. Biocontrol potential of native Trichoderma isolates against root-knot nematodes in West African vegetable production systems. Soil Biol Biochem. 2011;43(3):600–8.

    Article  CAS  Google Scholar 

  24. Martínez-Medina A, Fernandez I, Lok GB, Pozo MJ, Pieterse CM, Van Wees SC. Shifting from priming of salicylic acid-to jasmonic acid-regulated defences by Trichoderma protects tomato against the root knot nematode Meloidogyne incognita. New Phytol. 2017;213(3):1363–77.

    Article  PubMed  CAS  Google Scholar 

  25. Saikia SK, Tiwari S, Pandey R. Rhizospheric biological weapons for growth enhancement and Meloidogyne incognita management in Withania somnifera cv. Poshita. Biol Control. 2013;65(2):225–34.

    Article  Google Scholar 

  26. Zhao D, Zhao H, Zhao D, Zhu X, Wang Y, Duan Y, Xuan Y, Chen L. Isolation and identification of bacteria from rhizosphere soil and their effect on plant growth promotion and root-knot nematode disease. Biol Control. 2018;119:12–9.

    Article  CAS  Google Scholar 

  27. Dababat AA, Sikora RA, Hauschild R. Use of Trichoderma harzianum and Trichoderma viride for the biological control of Meloidogyne incognita on tomato. Commun Agric Appl Biol Sci. 2006;71(3 Pt B):953–61.

    CAS  PubMed  Google Scholar 

  28. Sharon E, Bareyal M, Chet I, Herreraestrella A, Kleifeld O, Spiegel Y. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology. 2001;91(7):687–93.

    Article  CAS  PubMed  Google Scholar 

  29. Zhang S, Gan Y, Xu B. Biocontrol potential of a native species of Trichoderma longibrachiatum against Meloidogyne incognita. Appl Soil Ecol. 2015;94:21–9.

    Article  Google Scholar 

  30. Hhmau H, Wijesundera R, Chandrasekharan N, Wijesundera W, Kathriarachchi H. Isolation and characterization of Trichoderma erinaceum for antagonistic activity against plant pathogenic fungi. Curr Res Environ Appl Mycol. 2015;5(2):120–7.

    Article  Google Scholar 

  31. Benitez T, Rincon AM, Limon MC, Codon AC. Biocontrol mechanisms of Trichoderma strains. Int Microbiol. 2004;7(4):249–60.

    CAS  PubMed  Google Scholar 

  32. Ragozzino A, d’Errico G. Interactions between nematodes and fungi: A concise review. Redia. 2011;94:123–5.

    Google Scholar 

  33. Lombardi N, Vitale S, Turra D, Reverberi M, Fanelli C, Vinale F, Marra R, Ruocco M, Pascale A, d’Errico G, Woo SL, Lorito M. Root exudates of stressed plants stimulate and attract Trichoderma soil fungi. Mol Plant Microbe Interact. 2018;31(10):982–94.

    Article  CAS  PubMed  Google Scholar 

  34. Xiao-Yan S, Qing-Tao S, Shu-Tao X, Xiu-Lan C, Cai-Yun S, Yu-Zhong Z. Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens. FEMS Microbiol Lett. 2006;260(1):119–25.

    Article  PubMed  CAS  Google Scholar 

  35. Hermosa R, Cardoza RE, Rubio MB, Gutiérrez S, Monte E. Secondary metabolism and antimicrobial metabolites of Trichoderma. In: Gupta VK, Schmoll M, Herrera-Estrella A, Upadhyay RS, Druzhinina I, Tuohy MG, editors. Biotechnol Biol Trichoderma. Chapter 10. Waltham: Elsevier; 2014. p. 125–37.

  36. Reino JL, Guerrero RF, Hernández-Galán R, Collado IG. Secondary metabolites from species of the biocontrol agent Trichoderma. Phytochemistry Rev. 2008;7(1):89–123.

    Article  CAS  Google Scholar 

  37. Favre-Bonvin J, Ponchet M, Djian C, Arpin N, Pijarowski L. Acetic acid: a selective nematicidal metabolite from culture filtrates of Paecilomyces lilacinus (Thom) Samson and Trichoderma longibrachiatum Rifai. Nematologica. 1991;37(1–4):101–12.

    Article  Google Scholar 

  38. Yang Z-S, Li G-H, Zhao P-J, Zheng X, Luo S-L, Li L, Niu X-M, Zhang K-Q. Nematicidal activity of Trichoderma spp. and isolation of an active compound. World J Microbiol Biotechnol. 2010;26(12):2297–302.

    Article  CAS  Google Scholar 

  39. Yang Z, Yu Z, Lei L, Xia Z, Shao L, Zhang K, Li G. Nematicidal effect of volatiles produced by Trichoderma sp. J Asia Pac Entomol. 2012;15(4):647–50.

    Article  CAS  Google Scholar 

  40. Suarez B, Rey M, Castillo P, Monte E, Llobell A. Isolation and characterization of PRA1, a trypsin-like protease from the biocontrol agent Trichoderma harzianum CECT 2413 displaying nematicidal activity. Appl Microbiol Biotechnol. 2004;65(1):46–55.

    Article  CAS  PubMed  Google Scholar 

  41. Chen L-L, Liu L-J, Shi M, Song X-Y, Zheng C-Y, Chen X-L, Zhang Y-Z. Characterization and gene cloning of a novel serine protease with nematicidal activity from Trichoderma pseudokoningii SMF2. FEMS Microbiol Lett. 2009;299(2):135–42.

    Article  CAS  PubMed  Google Scholar 

  42. Ling N, Xue C, Huang Q, Yang X, Xu Y, Shen Q. Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt. Biocontrol. 2010;55(5):673–83.

    Article  Google Scholar 

  43. Basyony AG, Abo-Zaid GA. Biocontrol of the root-knot nematode, Meloidogyne incognita, using an eco-friendly formulation from Bacillus subtilis, lab. and greenhouse studies. Egypt J Biol Pest Control. 2018;28:UNSP 87.

    Article  Google Scholar 

  44. Goswami J, Pandey RK, Tewari J, Goswami B. Management of root knot nematode on tomato through application of fungal antagonists, Acremonium strictum and Trichoderma harzianum. J Environ Sci Health Part B. 2008;43(3):237–40.

    Article  CAS  Google Scholar 

  45. Azarmi R, Hajieghrari B, Giglou A. Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. Afr J Biotechnol. 2011;10(31):5850–5.

    CAS  Google Scholar 

  46. Cai F, Yu G, Wang P, Wei Z, Fu L, Shen Q, Chen W. Harzianolide, a novel plant growth regulator and systemic resistance elicitor from Trichoderma harzianum. Plant Physiol Biochem. 2013;73:106–13.

    Article  CAS  PubMed  Google Scholar 

  47. Angel Contreras-Cornejo H, Macias-Rodriguez L, Cortes-Penagos C, Lopez-Bucio J. Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiol. 2009;149(3):1579–92.

    Article  CAS  Google Scholar 

  48. Li R-X, Cai F, Pang G, Shen Q-R, Li R, Chen W. Solubilisation of phosphate and micronutrients by Trichoderma harzianum and its relationship with the promotion of tomato plant growth. PLoS One. 2015;10(6):e0130081.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  49. Marra R, Lombardi N, d’Errico G, Troisi J, Scala G, Vinale F, Woo SL, Bonanomi G, Lorito M. Application of Trichoderma strains and metabolites enhances soybean productivity and nutrient content. J Agric Food Chem. 2019;67(7):1814–22.

    Article  CAS  PubMed  Google Scholar 

  50. Pradhan N, Sukla LB. Solubilization of inorganic phosphates by fungi isolated from agriculture soil. Afr J Biotechnol. 2006;5(10):850–4.

    CAS  Google Scholar 

  51. Martinuz A, Zewdu G, Ludwig N, Grundler F, Sikora RA, Schouten A. The application of Arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes. Planta. 2015;241(4):1015–25.

    Article  CAS  PubMed  Google Scholar 

  52. Cayrol JC, Djian C, Pijarowski L. Study of the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Rev Nematol. 1989;12(4):331–6.

    Google Scholar 

  53. Pandian RTP, Raja M, Kumar A, Sharma P. Morphological and molecular characterization of Trichoderma asperellum strain Ta13. Indian Phytopathol. 2016;69(3):297–303.

    Google Scholar 

  54. Kim JY, Kwon HW, Tang L, Kim SH. Identification and characterization of Trichoderma citrinoviride isolated from mushroom fly-infested oak log beds used for shiitake cultivation. Plant Pathol J. 2012;28(2):219.

    Article  Google Scholar 

  55. Kumar K, Amaresan N, Bhagat S, Madhuri K, Srivastava RC. Isolation and characterization of Trichoderma spp. for antagonistic activity against root rot and foliar pathogens. Indian J Microbiol. 2012;52(2):137–44.

    Article  CAS  PubMed  Google Scholar 

  56. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc Guide Methods Appl. 1990;18(1):315–22.

    Google Scholar 

  57. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24(8):1596–9.

    Article  CAS  PubMed  Google Scholar 

  58. Saharan V, Sharma G, Yadav M, Choudhary MK, Sharma S, Pal A, Raliya R, Biswas P. Synthesis and in vitro antifungal efficacy of Cu–chitosan nanoparticles against pathogenic fungi of tomato. Int J Biol Macromol. 2015;75:346–53.

    Article  CAS  PubMed  Google Scholar 

  59. Tiwari S, Pandey S, Chauhan PS, Pandey R. Biocontrol agents in co-inoculation manages root knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] and enhances essential oil content in Ocimum basilicum L. Ind Crops Prod. 2017;97:292–301.

    Article  Google Scholar 

  60. Barker KR. Design of greenhouse and microplot experiments for evaluation of plant resistance to nematodes. In: Zuckerman BM, Mai WF, Harrison MB, editors. Plant Nematol Lab Man. Amherst: University of Massachussets Agriculture Experiment Station; 1985. p. 103–13.

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Acknowledgments

We thank Prof. Yue Liang (Shenyang Agricultural University) for assistance on the identification of strain Snef1910 experiments. We also thank Tantan Gao (Beijing University of Agriculture) for comments during the preparation of this manuscript.

Funding

This work was financially supported by National Key Research and Development Program of China (Grant No. 2017YFD021104), Liaoning Doctoral Research Foundation Project (Grant No. 2019-BS-210), National Natural Science Foundation of China (Grant No. 31901927), and Educational Commission of Liaoning Province (Grant No. LSNQN201723). The funding bodies had no role in the design of the study, in the collection, analysis, interpretation of data, or in the writing of the manuscript.

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Authors and Affiliations

  1. Nematology Institute of Northern China, College of Plant Protection, Shenyang Agricultural University, Shenyang, China

    Haiyan Fan, Meiling Yao, Haiming Wang, Xiaofeng Zhu, Yuxi Duan & Lijie Chen

  2. Analytical and Testing Center, Shenyang Agricultural University, Shenyang, China

    Di Zhao

  3. College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, China

    Yuanyuan Wang

  4. College of Sciences, Shenyang Agricultural University, Shenyang, China

    Xiaoyu Liu

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  1. Haiyan Fan
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  2. Meiling Yao
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  3. Haiming Wang
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  4. Di Zhao
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  9. Lijie Chen
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Contributions

HYF carried out the identification of strain Snef1910 and Biocontrol of root-knot nematode in the field experiments, data collection and analysis, and wrote a manuscript draft. MLY carried out the main experiments and data collection. HMW participated in biocontrol of root-knot nematode in the field experiment. DZ participated in the identification of strain Snef1910. XFZ participated in experimental design and revised the manuscript. YYW participated in biocontrol of root-knot nematode in the pot experiment. XYL participated in biocontrol of root-knot nematode in the field experiment. YXD participated in experimental design and revised the manuscript. LJC guided experimental design. All authors read and approved the final manuscript.

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Correspondence to Lijie Chen.

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Supplementary information

Additional file 1: Table S1

. The effect of fermentation broth of 890 fungi strains on second stage juvenile (J2s) mortality of M. incognita in vitro at 24 h.

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Fan, H., Yao, M., Wang, H. et al. Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita. BMC Microbiol 20, 299 (2020). https://doi.org/10.1186/s12866-020-01984-4

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