Correction to: A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

Correction to: BMC Microbiol

https://doi.org/10.1186/s12866-018-1331-4

Following publication of the original article [1], we have been notified that three of the primer names identified as most promising candidates for fungal community surveys were incorrectly renamed following the primer nomenclature system proposed by Gargas & DePriest [2]. Their positioning on the reference sequence had to be shifted 1bp towards the 3’-end (see Table 1 for the correct naming). The same error occurred in some primer names listed in the additional files (see attached Supplementary information).

Table 1 Characteristics and in silico performance of the best primer pairs. Primer pairs were grouped according to the expected amplicon size into three groups: S for small (≤600 bp), M for medium (600 – 1,000 bp), and L for large size (>1,000 bp). Fungal and non-fungal eukaryotic sequence coverage rates tested by in silico PCR. Individual primer sequence and characteristics are listed in the Additional file 1. For primer pairs see Additional file 2

As consequence, the number of identical nucleotides shared by the most promising primers and the newly designed blocking oligo sequences changed (see Table 2).

In this correction, the revised supplementary materials are included.

Table 2 Characteristics of the best blocking oligonucleotides complementing the primer pair nu-SSU-1334-5’/nu-SSU-1648-3’ (FF390/FR-1). Fungal and non-fungal eukaryotic sequence coverage rate tested by in silico analysis