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Table 2 Characteristics of the best blocking oligonucleotides complementing the primer pair nu-SSU-1334-5’/nu-SSU-1648-3’ (FF390/FR-1). Fungal and non-fungal eukaryotic sequence coverage rate tested by in silico analysis

From: Correction to: A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

TargetSequenceComPrim#ntTm
(°C)
Fungi
(%)
Alv.
(%)
Rhiz.
(%)
Stram.
(%)
Tel.
(%)
Alveolatagtcgctcctaccgattganu-SSU-1648-3ˊ1250.30.0852.66.30.93.3
Rhizariattaacgaacgagacctcganu-SSU-1334-5ˊ1648.90024.30.30
Stramenopilestcgcacctaccgattgaanu-SSU-1648-3ˊ1348.300.50.377.11.7
Telonemagaccttaacctactaaatagttanu-SSU-1334-5ˊ548.100.30039.2
  1. ComPrim, sequence complement to the indicated primer.
  2. #nt, number of identical nt´s shared by primer and blocking oligo sequence
  3. Tm, annealing temperature
  4. %Fungi, coverage rate for fungal sequences
  5. %Alv., coverage rate for Alveolata sequences
  6. %Rhiz., coverage rate for Rhizaria sequences
  7. %Stram., coverage rate for Stramenopiles sequences
  8. %Tel., coverage rate for Telonema sequences