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Erratum to: The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway
BMC Microbiology volume 17, Article number: 186 (2017)
Erratum
After publication of our article [1] we became aware that two errors had been introduced during the revision process. These errors affect two figures (Fig. 2 and Fig. 4):
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In Fig. 2B the control panels with the non-transformed cells were wrong.
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In Fig. 4A the right panel (+CheW) was wrong.
Chemotaxis of E. coli is inhibited by the expression of Ppr or Pph. a The chemotactic wild type strain E. coli MM500 was transformed with the plasmids pBAD-Ppr (lanes 1 and 2), pBAD-Pph (lanes 3 and 4) and pBAD-Pph H670A (lanes 5 and 6). Cells were grown in TB medium to an OD600 = 0.5, 0.2% fructose (lanes 1, 3 and 5) or 0.2% arabinose (lanes 2, 4 and 6) was added, and growth was continued for 3 h. Protein expression was analyzed by SDS-PAGE and Coomassie blue staining. The positions of molecular weight markers are indicated. b TB swarm agar plates containing either 0.2% arabinose (upper panels) or 0.2% fructose (lower panels) were inoculated with E. coli MM500 cells bearing the plasmids pBAD-Ppr, pBAD-Pph and pBAD-Pph H670A, pBAD (vector without insert) or pBAD-KdpE. To develop chemotactic rings the plates were incubated for 6 h at 37 °C
Interaction between Pph and the chemotactic protein Rc-CheW. a The binding of the histidine kinase domain Pph and CheW was analyzed in pull-down assays. R. centenaria 6his-Rc-CheW was expressed in E. coli C41 cells and purified. The Pph protein was translated in vitro in the presence of [35S]-methionine (lane 1 and 4). Rc-CheW was added (50 μg) to the reaction and incubated at 37 °C. The sample was applied to a Cu-Sepharose column and after washing the bound complexes were eluted (lanes 3 and 6). The fractions were analysed by phosphorimaging. The in vitro translating protein extracts are shown in lanes 1 and 4, the final wash steps in lanes 2 and 5 and the elution fractions in lanes 3 and 6, respectively. The co-elution rate was calculated and is indicated. The positions of molecular weight markers are indicated. b The binding of the Pph protein and Rc-CheW was analysed in the presence of ATP. The Pph protein was translated and Rc-CheW was added as described in (A). ATP or apyrase was added to each reaction as indicated and the samples were analysed as described in (A). The co-elution rate was calculated and is indicated in % as bound Pph protein
Neither error changes the outcome of the experiments or the conclusions of the article. The corrected figures are shown as follows:
Reference
Kreutel S, Kuhn A, Kiefer D. The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway. BMC Microbiol. 2010;10(1):281.
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The online version of the original article can be found under doi:10.1186/1471-2180-10-281
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Kreutel, S., Kuhn, A. & Kiefer, D. Erratum to: The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway. BMC Microbiol 17, 186 (2017). https://doi.org/10.1186/s12866-017-1071-x
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DOI: https://doi.org/10.1186/s12866-017-1071-x