- Research article
- Open Access
Physiological roles of sigma factor SigD in Corynebacterium glutamicum
BMC Microbiologyvolume 17, Article number: 158 (2017)
Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown.
In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope.
This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.
Sigma factors are a component of bacterial RNA polymerase holoenzymes essential for promoter recognition and transcription initiation . Most bacteria encode multiple sigma factors, and each sigma factor containing RNA polymerase holoenzyme initiates transcription from the cognate promoter sequences [2,3,4,5]. By replacing a sigma factor in RNA polymerase holoenzyme, bacteria activate transcription of a different gene set under different conditions, and cope with environmental changes . Therefore, sigma factors play an important role in transcriptional regulation in a global manner. The knowledge of regulations by sigma factors is helpful to elucidate the regulatory network of the organism.
Corynebacterium glutamicum was first isolated as an organism secreting high amounts of L-glutamate . Nowadays, this bacterium is used for production of L-amino acids in million tons per year, especially L-glutamate and L-lysine . C. glutamicum ATCC 13032 has seven sigma factor genes in its chromosome, sigA, sigB, sigC, sigD, sigE, sigH and sigM [9, 10]. The physiological functions of SigA, SigB, SigC, SigE, SigH and SigM have been studied to some extent ; however, the regulation and physiological roles of SigD in C. glutamicum have not yet been revealed. sigD gene is well conserved among corynebacteria, and 17 out of 19 examined Corynebacterium species possess sigD genes . Therefore, it is assumed that SigD plays a substantial role in transcriptional regulation and subsequent adaptation of C. glutamicum to changing environments.
C. glutamicum belongs to the CMN (C orynebacterium, M ycobacterium, N ocardia) group, which is characterized by the unique molecular constituents of their cell envelopes such as the mycomembrane . The mycomembrane is composed of a monolayer of corynomycolate (α-alkyl, β-hydroxy fatty acid), which is covalently linked to arabinogalactan or forms other lipids such as trehalose monocorynomycolate (TMCM) and trehalose dicorynomycolate (TDCM) .
In this work, we evaluated the effects of deletion and overexpression of sigD on the cell phenotype, and revealed the influence of sigD overexpression on transcripts, proteins and metabolites. The achieved results disclosed the important roles of SigD in mycomembrane synthesis and maintaining cell wall integrity.
Bacterial strains, plasmids and oligonucleotides
The strains, plasmids and oligonucleotides used in this work are listed in Additional file 1: Table S1. A plasmid for sigD overexpression was constructed based on pVWEx1, which is an IPTG inducible expression vector for E. coli and C. glutamicum . A plasmid for gene disruption was constructed based on pK18mobsacB . For plasmid construction, DNA fragments were amplified from the genomic DNA of C. glutamicum ATCC 13032 by PCR with the oligonucleotide pairs shown in Additional file 1: Table S1. These fragments were inserted into the digested plasmid by ligation or Gibson assembly [16, 17]. E. coli DH5α was used for cloning. E. coli competent cells were transformed by the heat shock method  or by the electroporation method . DNA sequences of all cloned DNA fragments were confirmed to be correct by sequencing. C. glutamicum competent cells were transformed by electroporation at 2.5 kV, 200 Ω, and 25 μF [8, 19]. Gene disruption via two-step homologous recombination and the following selections were carried out as previously described . Disruption was verified by PCR with the respective oligonucleotide pairs.
Medium and conditions for growth experiments
Unless otherwise specified, C. glutamicum cells were precultured overnight in lysogeny broth (LB) medium  supplemented with 56 mM of glucose, washed once with chemically defined CGXII medium  without carbon source, and then inoculated into CGXII medium with 222 mM of glucose at an initial OD600 of 1. The cultivation was performed at 30 °C, 120 rpm. OD600 was measured with UV-1202 spectrophotometer (Shimadzu, Duisburg, Germany) with suitable dilutions. When necessary, 25 μg/mL of kanamycin and appropriate concentrations of IPTG were added as indicated in the text. For the growth experiment in BioLector® cultivation system (m2pLabs, Baesweiler, Germany), cells were cultivated in 1 mL of CGXII medium with 222 mM of glucose using FlowerPlate® (m2pLabs, Baesweiler, Germany) at 30 °C, 1100 rpm. Cell growth was monitored online every 10 min, and the maximum growth rate (h−1) was determined from the growth rates μ (h−1) which were calculated with regression analysis from backscattering light intensity (wavelength of 620 nm) at 20 consecutive measuring points.
Photometric determination of supernatant turbidity and observation of cell aggregation by microscopy
To quantify turbidity of supernatants, cell cultures were centrifuged for 30 min with 15,000 x g at room temperature. The absorption of supernatants was measured at a wavelength of 600 nm. For microscopic imaging, the culture of each strain in the stationary phase was diluted in CGXII medium without carbon source to an OD600 of 1, and observed by microscopy with 100 x oil immersion objective lens and 10 x ocular lens. Quantification of cell aggregate size was performed using ImageJ (https://imagej.nih.gov/ij/).
Protein analysis of the supernatant
Supernatants were taken from the stationary phase cultures. Four volumes of acetone were added to one volume of supernatant, and stored at −20 °C overnight. After centrifugation at 4 °C, 20,000 x g for 15 min, precipitates were resuspended in 20 mM Tris-HCl (pH 7). SDS-PAGE was performed using Tris-glycine discontinuous buffer, and visualized by staining with Coomassie Brilliant Blue R250 as previously described . Quantification of the intensity of each band was performed using ImageJ. Protein bands with different intensities observed in the control strain and the sigD overexpressing strain were excised from SDS-PAGE gels and transferred to a new tube which had been washed with trifluoroacetic acid: acetonitrile: H2O (0.1:60:40 v/v) in advance. The digestion of protein in the excised band was performed with trypsin overnight as previously described . Protein sequences were identified using an ultrafleXtreme MALDI-TOF/TOF mass spectrometry (Bruker, Bremen, Germany) and Mascot search engine (Matrix Science, London, UK) as previously described with some modifications for C. glutamicum ATCC 13032 .
Quantification of carbohydrate in acetone precipitates
Supernatants were precipitated with acetone as described above. Precipitates were resuspended with 100 μL of 20 mM Tris-HCl (pH 7), placed at room temperature for 30 min, and then the insoluble fraction was recovered by centrifugation. Insoluble fractions were hydrolyzed as previously described . Briefly, the precipitates were resuspended with 75 μL of 72% (w/w) sulfuric acid and incubated at room temperature for 3 h. The slurry was diluted to 1 mL with water, heated at 100 °C for 4 h, and cooled down on ice. Colorimetric quantification for carbohydrates was performed by the phenol sulfuric acid method as previously described with some modifications [23, 24]. Briefly, 200 μL of hydrolysate was mixed with 600 μL of concentrated sulfuric acid rapidly, and 120 μL of 5% phenol (w/v) in water was added immediately. The mixture was incubated for 5 min at 90 °C, and cooled to room temperature for 5 min. The absorption at 490 nm was measured, and compared to the absorption of the control samples with different concentrations of arabinose.
Cells were first precultured in LB medium, and inoculated in CGXII medium with 222 mM of glucose for adaptation. Then, the appropriate amount of cell culture was inoculated into fresh CGXII medium with 222 mM of glucose at an initial OD600 of 1. The cells were harvested at an OD600 between 6 and 8. Cell cultures (1 mL) were centrifuged for 30 s at 20,000 x g, and immediately frozen with liquid nitrogen after removing supernatant. RNA isolation was performed using the RNeasy mini kit along with the RNase-free DNase set (Qiagen, Hilden, Germany) as previously described . The absence of contaminating genomic DNA in RNA samples was confirmed by PCR with multiple oligonucleotide pairs specific to genomic DNA.
RNA isolation, library preparation and RNA-seq
For transcriptome sequencing, RNA samples isolated individually from biological triplicates were mixed for each stain. Quality check of the isolated RNA, library preparation, RNA-seq and data analysis were performed as previously described . Briefly, RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and Agilent RNA Nano 6000 kit with Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Removal of rRNA was checked by Agilent RNA Pico 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). TruSeq Stranded mRNA Library Prep Kit from Illumina (San Diego, CA, USA) was used to prepare cDNA libraries. cDNAs were sequenced paired end on an Illumina MiSeq system (San Diego, CA, USA) using 50 bp read length.
Read mapping, data visualization and analysis of gene expression
Trimmed reads (26 nt) were mapped to the C. glutamicum ATCC 13032 reference genome sequence  with SARUMAN , allowing for up to one error per read. The forward and reverse read, if both present and with a maximum distance of 1 kb, were combined to one read that contains the reference sequence as insert. Paired mappings with a distance >1 kb were discarded, and paired reads with either only the forward or only the reverse read mapping were retained as single mapping reads, as previously described . ReadXplorer 2.2.0 was used for visualization of short read alignments . For differential gene expression analysis, ReadXplorer and Bioconductor package DESeq implemented in ReadXplorer were used [29, 30]. Genes with the mean value of signal intensity less than 30 were discarded. A-value and M-value of each gene were calculated based on the intensity value of the strain of interest and the control strain.
Real-time PCR analysis
For real-time PCR analysis, relative abundance of mRNA of each gene was quantified using the same amount of total RNA. RNA sample extracted from biological triplicates were quantified individually. The experiment and analysis were performed as previously described with respective oligonucleotide pairs in Additional file 1: Table S1 .
Detection of trehalose dicorynomycolate by thin layer chromatography
Lipid extraction and thin layer chromatography (TLC) were performed based on the method previously described [32, 33]. Briefly, the crude lipid was extracted from cell pellet with CHCl3/CH3OH (1:1 v/v) once, and with CHCl3/CH3OH (2:1 v/v) for three times. All the extracts were pooled for each strain, and mixed with CHCl3/CH3OH/H2O (8:4:2 v/v) resulting the aqueous layer and the organic layer. The lower organic layer was collected and evaporated to dryness. The dried lipid was weighed and resuspended with CHCl3/CH3OH (4:1 v/v) to the same concentration for each sample. TLC was performed with ALUGRAM SIL G/UV254 (Macherey-Nagel, Germany) with CHCl3/CH3OH/H2O (30:8:1 v/v) as a developing solvent. Total lipid (600 μg) was developed for each strain, and the bands were visualized by spraying TLC plate with sulfuric acid and heating to 110 °C. The mobility of TDCM or TMCM was determined by the retardation factor (Rf) value from the previous studies [32, 33]. Quantification of the intensity of each band was performed with ImageJ.
Deletion and overexpression of sigD influenced the maximum growth rate
The sigD deletion mutant (ΔsigD) and the sigD overexpressing strain were cultivated in CGXII medium containing 222 mM of glucose as carbon source. sigD was overexpressed from the plasmid pVWEx1-sigD using an IPTG inducible promoter and different IPTG concentrations (0, 10, 50, 250 or 1000 μM). The ΔsigD mutant grew slightly slower than the wild type (WT) strain (Fig. 1a). The maximum growth rate of the sigD overexpressing strain WT(pVWEx1-sigD) decreased in an IPTG-dependent manner, which was not observed for WT(pVWEx1) (Fig. 1b). Higher concentrations of IPTG (250 and 1000 μM) severely inhibited the growth of WT(pVWEx1-sigD). The final biomass concentrations after 24 h of cultivation were comparable for WT, ΔsigD, WT(pVWEx1) and WT(pVWEx1-sigD) with 0, 10 or 50 μM of IPTG (data not shown). The slower growth of the ΔsigD strain indicated that sigD is beneficial for growth in minimal CGXII medium although it is not essential for growth under optimum conditions. As excessive sigD overexpression at the high IPTG concentrations was found to be harmful to cells, induction with 50 μM of IPTG was used for further experiments.
sigD overexpression influenced cell culture characteristics
WT(pVWEx1-sigD) with 50 μM of IPTG showed distinct characteristics compared to WT(pVWEx1) as the cultures of WT(pVWEx1-sigD) foamed significantly less than those of WT(pVWEx1) (Fig. 2a). Cell cultures with different IPTG concentrations (0, 10, 50 μM) using FlowerPlates and a BioLector system revealed that the supernatants of WT(pVWEx1-sigD) cultures showed higher turbidity than those of the control strain, and this turbidity increased in an IPTG-dependent manner (Fig. 2b). In addition to these distinct characteristics of the cell culture, cell aggregation was observed under the microscope in WT(pVWEx1-sigD) with IPTG (Fig. 2c–e). Taken together, overexpression of sigD induced pleiotropic changes of the phenotype of C. glutamicum.
sigD overexpression changed the pattern of secreted proteins
Overexpression of sigD influenced the culture characteristic of foaming. Therefore, the profile of proteins in the culture supernatant was compared between WT(pVWEx1) and WT(pVWEx1-sigD). Proteins in the supernatants were analyzed by 1D SDS-PAGE after concentrating by acetone precipitation. We observed different band patterns of proteins secreted by WT(pVWEx1) and WT(pVWEx1-sigD) (Fig. 3a). The proteins in the bands with different intensity were further characterized by tryptic digestion and MALDI-TOF/TOF mass spectrometry (Fig. 3b, c). In this way, corynomycolyl transferase Cmt1 was identified in the band 3, which showed a higher intensity when sigD was overexpressed. The band 2 was identified as L, D-transpeptidase LppS, and the band 4 was identified as mixture of two proteins, corynomycolyl transferase Cmt2 and putative secreted protein Cg2052. On the other hand, Psp3, which was found in the band 1 and annotated as putative secreted protein, was less abundant in WT(pVWEx1-sigD). These results demonstrated that overexpression of sigD altered the secreted protein profile. Since Cmt1 abundance in the supernatant increased most upon sigD overexpression (Fig. 3a, c), cmt1 was overexpressed in the WT strain. However, less foaming was not observed under these conditions (data not shown). Therefore, we concluded that less foaming of the culture did not occur only due to Cmt1 protein abundance but to complex or other unknown reasons.
sigD overexpression induced the secretion of carbohydrate-containing compounds
During acetone precipitation of the supernatant, we observed an insoluble fraction only in the sample of the sigD overexpressing strain. This insoluble fraction still existed after an overnight protease K treatment. For the strain C. glutamicum CGL2005, it was reported that the ethanol-precipitated fraction of extracellular components consisted primarily of carbohydrates . Therefore, the carbohydrate content in this fraction was determined by the phenol sulfuric acid method , which detects all classes of carbohydrates . Arabinose, which is one of the components of peptidoglycan in C. glutamicum, was used as control. Colorimetric determination after hydrolysis confirmed that the insoluble fraction of WT(pVWEx1-sigD) supernatants contained carbohydrates (arabinose equivalent of 1.3 mM). On the other hand, the same treatment of WT, ΔsigD or WT(pVWEx1) supernatants resulted in carbohydrate contents below the detection limit (arabinose equivalent <0.1 mM) (Table 1). These results showed that sigD overexpression induced the secretion of polysaccharides or carbohydrate-containing compounds into the supernatant.
SigD regulated transcription of several genes related to cell envelope integrity
RNA-seq and real-time PCR analysis were performed to understand the effects caused by sigD overexpression or deletion at the transcriptional level. The relative abundance of mRNA of each gene was compared in WT(pVWEx1-sigD) without IPTG or with 50 μM of IPTG to analyze the effects of sigD overexpression. On the other hand, the mRNA abundance was compared between the ΔsigD and the WT strain to study the effects of sigD deletion. First, RNA - seq analysis was applied to screen the genes whose expression levels changed upon sigD overexpression or deletion. RNA-seq analysis implied that expression of 29 genes increased upon sigD overexpression (M-value >1) (Additional file 2: Table S2). Of these 29 genes, several genes are annotated as genes related to cell wall integrity; 6 genes (cop1, cmt1, cmt2, cmt3, elrF and fadD2) as corynomycolyl related proteins, a gene annotated as L, D-transpeptidase (lppS) and three genes (cg0420, cg0532, cg1181) as glycosyltransferases. In addition, expression of two further genes encoding proteins with mycomembrane-related functions, porH (cg3009) and pks (cg3178), increased upon sigD overexpression (M-value >log2(1.5)) and slightly decreased in the ΔsigD strain (M-value <−log2(1.5)). Based on these RNA-seq results, the expression of those genes was further analyzed by real-time PCR (Fig. 4). All of the tested genes were shown to be upregulated significantly as consequence of sigD overexpression. Increased transcript levels of cmt1, cmt2 and lppS were consistent with the increased protein levels of Cmt1, Cmt2 and LppS in culture supernatants upon sigD overexpression (Fig. 3b, c). These results showed that the function of SigD is related to the regulation of cell envelope integrity such as mycomembrane synthesis and cell wall synthesis.
Overexpression of sigD increased the amounts of trehalose dicorynomycolate
The results of the real-time PCR analysis confirmed that the expression of several genes annotated as corynomycolyl transferase (cop1, cmt1, cmt2, cmt3) and genes related to corynomycolic acid production (pks and fadD2) increased due to sigD overexpression. Overexpression of cop1 is known to increase the trehalose dicorynomycolate (TDCM) content in the cells . To confirm the effect of sigD overexpression on the TDCM content, the crude lipid was extracted from cells, and its composition was analyzed by TLC (Fig. 5). The intensity of the band corresponding to TDCM increased 39% upon sigD overexpression. On the contrary, the intensity of the band corresponding to trehalose monocorynomycolate (TMCM) did not show a notable difference between the two strains. This result indicates that upregulation of multiple genes by sigD overexpression increased the flux toward corynomycolic acid synthesis which resulted in an alteration of TMCM/TDCM ratio as well as accumulation of other carbohydrate containing compounds.
Understanding the regulatory mechanisms of bacteria is important in many fields varying from biotechnology to public health. C. glutamicum has been used for the production of amino acids for several decades, however, the transcriptional regulation by sigma factors has not been fully elucidated. As for SigD, selection of high oxygen requiring mutants in a transposon library accidentally revealed that deletion of sigD in C. glutamicum loses the ability to grow under low oxygen concentrations , however, the knowledge is still limited. In the previous works, we demonstrated that overexpression of one of global regulators can artificially perturb the cellular regulation and influence metabolites as well as transcripts [37, 38]. This approach of sigma factor gene overexpression was found to be a useful approach for investigation of regulatory mechanisms and activation of specific biosynthesis pathways in C. glutamicum. In this work, deletion and overexpression of sigD revealed that sigma factor SigD plays a role in regulating maintenance of the cell wall integrity in C. glutamicum (Fig. 6).
The real time PCR analysis revealed that overexpression of sigD induced the expression of multiple genes related to mycomembrane synthesis (cop1, cmt1, cmt2, cmt3, elrF, fadD2, porH and pks) which exist at the different loci in the C. glutamicum genome. For mycomembrane biosynthesis, one molecule of fatty acid is carboxylated via the carboxylation complex composed of AccD2, AccD3, AccBC and AccE , and a second fatty acid is activated to a fatty acyl-CoA by FadD2 . These two molecules are condensed and attached to trehalose by Pks [41, 42]. This product is reduced to TMCM (trehalose monocorynomycolate) by CmrA , and TMCM is exported from cytoplasm [44, 45]. Then, corynomycolyl transferases transfer the corynomycolate group of TMCM onto arabinogalactan, TMCM itself or proteins such as PorH [32, 33, 46, 47]. C. glutamicum possesses six corynomycolyl transferase genes, cop1, cmt1, cmt2, cmt3, cmt4 and cmt5 , and Cop1, Cmt1 and Cmt2 catalyze TDCM synthesis from TMCM [32, 46]. Cop1 is also reported to transfer corynomycolate from TMCM to arabinogalactan in the cell wall in C. glutamicum CGL2005 . Interestingly, sigD overexpression induced the expression of four out of six corynomycolyl transferase genes at the same time, and enhanced the secretion of Cmt1 and Cmt2 to the supernatant. PorH forms the major cell wall channel penetrating the mycomembrane together with the other protein PorA , and corynomycolation of PorH and PorA catalyzed by Cmt1 was shown to be necessary for the pore forming activity [47, 49]. Furthermore, ElrF was identified as the envelope lipids regulation factor which regulates lipid composition of corynomycolic acids and phospholipids in cell envelope . In this study, increased content of TDCM in the crude lipid extract indicated that sigD overexpression influences not only the transcription of those genes but also the metabolic flux toward mycomembrane synthesis (Fig. 6). These results indicate that SigD controls the integrity of the cell envelope, especially of the mycomembrane in C. glutamicum.
SigD of C. glutamicum is classified as ECF40 type sigma factor by the ECFfinder program , as is SigD of M. tuberculosis. SigD in M. tuberculosis was shown to be essential for virulence, and inactivation of sigD decreased expression of some mycolyl transferase genes as well as other genes related to lipid metabolism and cell wall processes [51, 52]. For example, Calamita et al. reported that the expression of fbpA encoding antigen 85A, which is a homolog for corynomycolyl transferase, decreased in the sigD deletion strain . Raman et al. showed that the expression of fbpC encoding antigen 85C, which is also a homolog for corynomycolyl transferase, decreased two-fold in the sigD deletion strain . Even though a different organism has a different regulatory architecture, C. glutamicum and M. tuberculosis may share the similar regulatory network by SigD.
SigD gene overexpression in C. glutamicum led to excretion of carbohydrate-containing compounds and cell aggregation. In C. glutamicum CGL2005, various types of polysaccharides were detected extracellularly [34, 53]. In addition, C. glutamicum CCTCC M201005 was found in soil as a producer of a bioflocculant consisting of galacturonic acid as the main structural unit . In M. tuberculosis and M. smegmatis, polysaccharides containing arabinose are suggested to be involved in the aggregation of cells via interaction with Antigen 85s, which are homologs for corynomycolyl transferases . Furthermore, arabinose was shown to promote the cell aggregation of M. smegmatis . Therefore, excretion of polysaccharides or carbohydrate-containing compounds caused by sigD overexpression may induce cell aggregation also in C. glutamicum.
Sigma factors regulate transcription in a global manner and their effects are pleiotropic. In this study, the link between sigD overexpression and the regulation of cell wall integrity was confirmed, however, other effects remain to be elucidated, if exist. Furthermore, overexpression of sigD turns on the cascade regulation which includes direct and indirect outcomes inside the cells. Based on our results, the physiological role of C. glutamicum SigD in cell wall integrity seems apparent, however, the elucidation of the SigD regulon control and definition of the class of SigD-dependent promoters will require further molecular studies.
The non-pathogenic C. glutamicum serves as a good model organism for understanding the cell wall biosynthesis and resistance to antibiotics in Corynebacterineae, which include human pathogenic bacteria such as M. tuberculosis and C. diphtheriae . Therefore, the findings described in this work can be helpful to understand the cell wall biosynthesis of Corynebacterineae. Furthermore, TDCM was shown to induce priming and activation of macrophages in vivo and in vitro in a similar manner as TDM from M. tuberculosis . Chemical synthesis of TDM is not easy because it requires multiple steps, and those compounds are extracted from organisms. Considering the common biotechnological use of C. glutamicum, the production of TDCM with C. glutamicum seems to be an attractive idea. On the other hand, a corynomycolate-less strain is known to excrete more L-glutamate and L-lysine  as well as to take up glycerol and acetate more efficiently . Reorganization of the mycomembrane by controlling sigD expression can be therefore helpful to understand the permeability barrier of C. glutamicum cells, and construct strains with higher or lower permeability barriers.
In this work, the functions of C. glutamicum sigma factor SigD were studied by overexpression or disruption of sigD gene. Overexpression of sigD led to the several physiological changes such as slower growth, cell aggregation, less foaming of the culture and increased turbidity of the supernatant. The real-time PCR analysis confirmed that overexpression of sigD induced the expression of several genes related to maintenance of cell envelop integrity and mycomembrane biosynthesis. Furthermore, overexpression of sigD increased the content of trehalose dicorynomycolate in the lipid extract.
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HT acknowledges support by DAAD (Deutscher Akademischer Austauschdienst) for providing a full PhD fellowship and MP acknowledges support by grant 17-06991S from Czech Science Foundation. Those foundations had no role in the design of the study in collection, analysis, and interpretation of data and in writing the manuscript.
Availability of data and materials
The datasets generated during the current study are available in the ArrayExpress database repository (E-MTAB-5411: http://www.ebi.ac.uk/arrayexpress/).
HT currently belongs to Synthetic Bioengineering lab, Dept.of Biotechnology, Graduate School of Engineering, Osaka University (Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan).
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Table S1. Bacterial strains, plasmids and oligonucleotides used in this work. Bacterial strains, plasmids and oligonucleotides used in this work are listed. (DOCX 17 kb)
Table S2. RNA-seq analysis of genes differentially transcribed upon sigD overexpression or sigD disruption. The name of genes which expression levels increased under sigD overexpression (M-value > 1.0) are listed. (DOCX 18 kb)