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Fig. 3 | BMC Microbiology

Fig. 3

From: Physiological roles of sigma factor SigD in Corynebacterium glutamicum

Fig. 3

1D-SDS PAGE of proteins in the supernatants. a Secreted proteins were analyzed by 12% SDS-PAGE. The molecular sizes of proteins in the marker are shown in kDa. WT(pVWEx1) and WT(pVWEx1-sigD) protein samples were obtained by acetone precipitation of the supernatants. Proteins from 200 μL of the supernatant was loaded on each lane. The intensity of secreted protein bands was quantified for WT(pVWEx1) (b) and for WT(pVWEx1-sigD) (c). The highest intensity of the band was normalized to 100%. The protein bands labeled with numbers were subjected to MALDI-TOF/TOF MS. 1: Psp3 (Cg2061), 2: LppS (Cg2720), 3: Cmt1 (Cg0413), 4: Cmt2 (Cg3186) and Cg2052

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