Singh N, Armstrong DG, Lipsky BA. Preventing foot ulcers in patients with diabetes. Jama. 2005;293(2):217–28.
Article
CAS
PubMed
Google Scholar
Bakker K, Apelqvist J, Schaper N. Practical guidelines on the management and prevention of the diabetic foot 2011. Diabetes Metab Res Rev. 2012;28(S1):225–31.
Article
PubMed
Google Scholar
Alexiadou K, Doupis J. Management of diabetic foot ulcers. Diabetes Therapy. 2012;3(1):1–15.
Article
Google Scholar
Boulton AJ, Vileikyte L, Ragnarson-Tennvall G, Apelqvist J. The global burden of diabetic foot disease. Lancet. 2005;366(9498):1719–24.
Article
PubMed
Google Scholar
Robson MC. Wound infection: a failure of wound healing caused by an imbalance of bacteria. Surg Clin North Am. 1997;77(3):637–50.
Article
CAS
PubMed
Google Scholar
James GA, Swogger E, Wolcott R, Secor P, Sestrich J, Costerton JW, Stewart PS. Biofilms in chronic wounds. Wound Repair Regen. 2008;16(1):37–44.
Mah T-FC, O'Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001;9(1):34–9.
Article
CAS
PubMed
Google Scholar
Hex N, Bartlett C, Wright D, Taylor M, Varley D. Estimating the current and future costs of type 1 and type 2 diabetes in the UK, including direct health costs and indirect societal and productivity costs. Diabet Med. 2012;29(7):855–62.
Article
CAS
PubMed
Google Scholar
Doupis J, Veves A. Classification, diagnosis, and treatment of diabetic foot ulcers. Wounds: a compendium of clinical research and practice. 2008;20(5):117–26.
Google Scholar
Oates A, Bowling FL, Boulton AJ, McBain AJ. Molecular and culture-based assessment of the microbial diversity of diabetic chronic foot wounds and contralateral skin sites. J Clin Microbiol. 2012;50(7):2263–71.
Article
CAS
PubMed
PubMed Central
Google Scholar
Gardner SE, Hillis SL, Heilmann K, Segre JA, Grice EA. The neuropathic diabetic foot ulcer microbiome is associated with clinical factors. Diabetes. 2013;62(3):923–30.
Article
CAS
PubMed
PubMed Central
Google Scholar
Consortium HMP. A framework for human microbiome research. Nature. 2012;486(7402):215–21.
Article
Google Scholar
Lavery LA, Armstrong DG, Wunderlich RP, Mohler MJ, Wendel CS, Lipsky BA. Risk factors for foot infections in individuals with diabetes. Diabetes Care. 2006;29(6):1288–93.
Article
PubMed
Google Scholar
Bjarnsholt T, Kirketerp‐Møller K, Jensen PØ, Madsen KG, Phipps R, Krogfelt K, Høiby N, Givskov M. Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen. 2008;16(1):2–10.
Xu L, McLennan SV, Lo L, Natfaji A, Bolton T, Liu Y, Twigg SM, Yue DK. Bacterial load predicts healing rate in neuropathic diabetic foot ulcers. Diabetes Care. 2007;30(2):378–80.
Martin JM, Zenilman JM, Lazarus GS. Molecular microbiology: new dimensions for cutaneous biology and wound healing. J Investig Dermatol. 2010;130(1):38–48.
Article
CAS
PubMed
Google Scholar
Stacy A, Everett J, Jorth P, Trivedi U, Rumbaugh KP, Whiteley M. Bacterial fight-and-flight responses enhance virulence in a polymicrobial infection. Proc Natl Acad Sci U S A. 2014;111(21):7819–24.
Article
CAS
PubMed
PubMed Central
Google Scholar
Stewart PS. Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol. 2002;292(2):107–13.
Article
CAS
PubMed
Google Scholar
Percival SL, Emanuel C, Cutting KF, Williams DW. Microbiology of the skin and the role of biofilms in infection. Int Wound J. 2012;9(1):14–32.
Article
PubMed
Google Scholar
Lipsky BA, Berendt AR, Cornia PB, Pile JC, Peters EJ, Armstrong DG, Deery HG, Embil JM, Joseph WS, Karchmer AW. 2012 infectious diseases society of America clinical practice guideline for the diagnosis and treatment of diabetic foot infections. Clin Infect Dis. 2012;54(12):e132–73.
Dang C, Prasad Y, Boulton A, Jude E. Methicillin‐resistant Staphylococcus aureus in the diabetic foot clinic: a worsening problem. Diabet Med. 2003;20(2):159–61.
Article
CAS
PubMed
Google Scholar
Citron DM, Goldstein EJ, Merriam CV, Lipsky BA, Abramson MA. Bacteriology of moderate-to-severe diabetic foot infections and in vitro activity of antimicrobial agents. J Clin Microbiol. 2007;45(9):2819–28.
Article
PubMed
PubMed Central
Google Scholar
Kaeberlein T, Lewis K, Epstein SS. Isolating” uncultivable” microorganisms in pure culture in a simulated natural environment. Science. 2002;296(5570):1127–9.
Article
CAS
PubMed
Google Scholar
Dowd SE, Wolcott RD, Sun Y, McKeehan T, Smith E, Rhoads D. Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP). PLoS One. 2008;3(10), e3326.
Article
PubMed
PubMed Central
Google Scholar
Peters BM, Jabra-Rizk MA, Graeme A, Costerton JW, Shirtliff ME. Polymicrobial interactions: impact on pathogenesis and human disease. Clin Microbiol Rev. 2012;25(1):193–213.
Article
PubMed
PubMed Central
Google Scholar
Grice EA, Segre JA. The skin microbiome. Nat Rev Microbiol. 2011;9(4):244–53.
Article
CAS
PubMed
PubMed Central
Google Scholar
Grice EA, Kong HH, Conlan S, Deming CB, Davis J, Young AC, Bouffard GG, Blakesley RW, Murray PR, Green ED. Topographical and temporal diversity of the human skin microbiome. Science. 2009;324(5931):1190–2.
Ghebremedhin B, Layer F, König W, König B. Genetic classification and distinguishing of staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences. J Clin Microbiol. 2008;46(3):1019–25.
Article
CAS
PubMed
PubMed Central
Google Scholar
Armstrong DG, Lanthier J, Lelievre P, Edelson GW. Methicillin-resistant coagulase-negative staphylococcal osteomyelitis and its relationship to broad-spectrum oral antibiosis in a predominantly diabetic population. J Foot Ankle Surg. 1995;34(6):563–6.
Article
CAS
PubMed
Google Scholar
Loïez C, Wallet F, Pischedda P, Renaux E, Senneville E, Mehdi N, Courcol RJ. First case of osteomyelitis caused by “Staphylococcus pettenkoferi”. J Clin Microbiol. 2007;45(3):1069–71.
Bessman AN, Geiger PJ, Canawati H. Prevalence of corynebacteria in diabetic foot infections. Diabetes Care. 1992;15(11):1531–3.
Article
CAS
PubMed
Google Scholar
Gontcharova V, Youn E, Sun Y, Wolcott RD, Dowd SE. A comparison of bacterial composition in diabetic ulcers and contralateral intact skin. The open microbiology journal. 2010;4:8.
Article
PubMed
PubMed Central
Google Scholar
Dunyach-Remy C, Cadière A, Richard J-L, Schuldiner S, Bayle S, Roig B, Sotto A, Lavigne J-P. Polymerase chain reaction—denaturing gradient gel electrophoresis (PCR–DGGE): a promising tool to diagnose bacterial infections in diabetic foot ulcers. Diabetes Metab. 2014;40(6):476–80.
Sibley CD, Peirano G, Church DL. Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology. Infect Genet Evol. 2012;12(3):505–21.
Article
CAS
PubMed
Google Scholar
Sun Y, Smith E, Wolcott R, Dowd S. Propagation of anaerobic bacteria within an aerobic multi-species chronic wound biofilm model. J Wound Care. 2009;18(10):426–31.
Article
CAS
PubMed
Google Scholar
Murphy EC, Mörgelin M, Reinhardt DP, Olin AI, Björck L, Frick IM. Identification of molecular mechanisms used by finegoldia magna to penetrate and colonize human skin. Mol microbiol. 2014;94(2):403–17.
Article
CAS
PubMed
PubMed Central
Google Scholar
Sivanmaliappan TS, Sevanan M. MAntimicrobial susceptibility patterns of Pseudomonas aeruginosa from diabetes patients with foot ulcers. Int j microbiol. 2011;2011:4.
Article
Google Scholar
Grice EA, Snitkin ES, Yockey LJ, Bermudez DM, Liechty KW, Segre JA, Mullikin J, Blakesley R, Young A, Chu G. Longitudinal shift in diabetic wound microbiota correlates with prolonged skin defense response. Proc Natl Acad Sci. 2010;107(33):14799–804.
Lipsky BA. Treating diabetic foot osteomyelitis primarily with surgery or antibiotics: have we answered the question? Diabetes Care. 2014;37(3):593–5.
Article
PubMed
Google Scholar
Edmonds M, Foster A. The use of antibiotics in the diabetic foot. Am J Surg. 2004;187(5):S25–8.
Article
Google Scholar
Löfmark S, Edlund C, Nord CE. Metronidazole is still the drug of choice for treatment of anaerobic infections. Clin infect dis. 2010;50(Supplement 1):S16–23.
Article
PubMed
Google Scholar
Aherrao N, Shahi SK, Dwivedi A, Kumar A, Gupta S, Singh SK. Detection of anaerobic infection in diabetic foot ulcer using PCR technique and the status of metronidazole therapy on treatment outcome. Wounds : a compendium of clinical research and practice. 2012;24(10):283–8.
Google Scholar
Clay PG, Graham MR, Lindsey CC, Lamp KC, Freeman C, Glaros A. Clinical efficacy, tolerability, and cost savings associated with the use of open-label metronidazole plus ceftriaxone once daily compared with ticarcillin/clavulanate every 6 h as empiric treatment for diabetic lower-extremity infections in older males. Am J Geriatr Pharmacother. 2004;2(3):181–9.
Article
CAS
PubMed
Google Scholar
Peters EJ, Armstrong DG, Lavery LA. Risk factors for recurrent diabetic foot ulcers: site matters. Diabetes Care. 2007;30(8):2077–9.
Article
PubMed
Google Scholar
O'Donnell LE, Smith K, Williams C, Nile CJ, Lappin DF, Bradshaw D, Lambert M, Robertson DP, Bagg J, Hannah V et al. Dentures are a reservoir for respiratory pathogens Journal of Prosthodontics 2015; doi:10.1111/jopr.12342.
Magoč T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics. 2011;27(21):2957–63.
Article
PubMed
PubMed Central
Google Scholar
Li W, Godzik A. Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006;22(13):1658–9.
Article
CAS
PubMed
Google Scholar
Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics. 2011;27(16):2194–200.
Article
CAS
PubMed
PubMed Central
Google Scholar
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403–10.
Article
CAS
PubMed
Google Scholar
Hammer OHD, Ryan PD. PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron. 2001;4(1):9.
Google Scholar