- Research article
- Open Access
The nucleotide excision repair (NER) system of Helicobacter pylori: Role in mutation prevention and chromosomal import patterns after natural transformation
BMC Microbiology volume 12, Article number: 67 (2012)
Extensive genetic diversity and rapid allelic diversification are characteristics of the human gastric pathogen Helicobacter pylori, and are believed to contribute to its ability to cause chronic infections. Both a high mutation rate and frequent imports of short fragments of exogenous DNA during mixed infections play important roles in generating this allelic diversity. In this study, we used a genetic approach to investigate the roles of nucleotide excision repair (NER) pathway components in H. pylori mutation and recombination.
Inactivation of any of the four uvr genes strongly increased the susceptibility of H. pylori to DNA damage by ultraviolet light. Inactivation of uvrA and uvrB significantly decreased mutation frequencies whereas only the uvrA deficient mutant exhibited a significant decrease of the recombination frequency after natural transformation. A uvrC mutant did not show significant changes in mutation or recombination rates; however, inactivation of uvrC promoted the incorporation of significantly longer fragments of donor DNA (2.2-fold increase) into the recipient chromosome. A deletion of uvrD induced a hyper-recombinational phenotype.
Our data suggest that the NER system has multiple functions in the genetic diversification of H. pylori, by contributing to its high mutation rate, and by controlling the incorporation of imported DNA fragments after natural transformation.
The human stomach pathogen Helicobacter pylori infects approximately 50% of the world population, usually from childhood until old age . H. pylori exhibits exceptionally high genetic diversity, such that almost every infected human carries one or multiple unique H. pylori strains [2, 3]. This diversity is the result of the combination of a high mutation rate with very efficient recombination during mixed infections with multiple strains [4–7], for reviews see [8–11]. The specific mechanisms that are responsible for the high mutation rate of H. pylori and the unusual characteristics of its DNA uptake and recombination machinery are yet incompletely understood.
We have previously described an in vitro system that allows us to measure mutation and transformation frequencies in H. pylori wild type strains and isogenic gene knock-out mutants, as well as the length of the donor DNA fragments imported into the recipient chromosome after transformation . In this system, natural transformation of different H. pylori wild type strains with DNA from heterologous H. pylori donors led to the incorporation of 1.3-3.8 kb fragments into the recipient chromosome, depending on the combination of donor and recipient strains. Imports resulting from recombination contained short interspersed sequences of the recipient (ISR) in ~10% of the cases [12, 13], leading to complex mosaic patterns. The glycosylase MutY, a member of the base excision repair (BER) machinery, is involved in at least one ISR-generating pathway in H. pylori, repairing mismatches after the heteroduplex formation between recipient and donor DNA . However, the inactivation of mutY in H. pylori did not completely abrogate the formation of ISR, suggesting that additional mechanisms might contribute to ISR generation.
In addition to BER, H. pylori also contains a second gap-filling DNA repair system, the nucleotide excision repair pathway (NER), whose role in H. pylori mutation and recombination is yet poorly understood. In Escherichia coli, the NER system is responsible for the replacement of bulky DNA lesions such as covalently modified bases, noncovalent drug nucleotide complexes and abasic sites generated by oxidative metabolism or ionizing radiation [14, 15]. Initiation of NER starts with the recognition of DNA distortions by the UvrAB complex . After recognition, UvrA dissociates and UvrC is recruited and acts as a single-stranded DNA endonuclease, cleaving at both sides of the lesion [17, 18]. Finally, the unwinding activity of the UvrD helicase, which preferentially catalyzes a 3’ to 5’ unwinding, removes the excised segment. DNA polymerase I fills in the gap while the remaining nick is closed by ligase [19, 20]. In H. pylori, orthologs of the four NER genes, uvrA-D, have been identified ; but until now, only few studies have addressed the functions of these genes. H. pylori UvrB was shown to be involved in the repair of acid-induced DNA damage , and UvrD limited homologous recombination and DNA damage-induced genomic rearrangements between DNA repeats .
Here we have used a genetic approach to analyze the roles of the H. pylori NER system components in regulating the mutation rate, and the frequency and import patterns of homologous recombination after natural transformation.
Characterization of H. pylori NER mutants and their susceptibility to UV light-induced cell damage
To investigate how the NER system contributes to genetic diversification in H. pylori, we individually inactivated the NER genes in H. pylori strain 26695 by either allelic disruption with a kanamycin resistance cassette (uvrA uvrB), a chloramphenicol resistance conferring cassette (uvrC), or by quasi-complete replacement with a kanamycin resistance (uvrD, see Methods for details). Since the components of the NER system participate in repairing damage caused by UV radiation in many different organisms , we first investigated the sensitivity of the diverse NER mutant strains against UV light. Mutants in uvrA, uvrB, uvrC and uvrD as well as a recA mutant  were exposed to UV irradiation and the amount of surviving cells was compared to the survival rate of the wt strain 26695. Inactivation of any of the NER components markedly increased the susceptibility to UV irradiation (Figure 1), indicating that all NER mutants are impaired in DNA repair.
To assess the effect of NER gene inactivation on growth properties in vitro, which might affect the results of other experiments reported in this study, growth curves were performed for all mutants and compared to wild type strain 26695. None of the NER mutants were affected in their growth properties in comparison with the wild type strain 26695 (Additional file 1: Figure S1).
Spontaneous mutation frequencies in NER deficient mutants
Since the control of spontaneous mutagenesis has been associated with the NER system in E. coli, we determined the effect of inactivating the NER genes on spontaneous mutation frequencies. For this experiment, the frequencies of mutations conferring rifampicin (Rif) resistance, occurring through different single base-pair mutations in the rpoB gene , were measured (Figure 2A). The inactivation of uvrA and uvrB significantly reduced the mutation frequency, while the inactivation of uvrC and uvrD had no significant effect on the frequency of Rif resistant mutants. In order to rule out that the observed effects of the inactivation of uvrA and uvrB were due to polar effects, we constructed complemented strains where an intact copy of the target gene was introduced into the chromosome of the mutant (see Methods for details). The introduction of intact gene copies restored the mutation rates of the mutant strains to wild type levels (Figure 2A).
Recombination frequencies in NER-deficient H. pylori mutants after natural transformation
We next examined the role of the H. pylori NER system in recombination. Each mutant strain was individually transformed with genomic DNA extracted from H. pylori strain J99-R3. This strain contains a point mutation (A1618T) that confers Rif resistance which can be used as a selection marker to recover recombinant clones (Additional file 2: Figure S2). Recombinant clones were distinguished from spontaneous mutants by partial rpoB sequence analysis. The uvrA mutant exhibited a highly significant decrease of the recombination frequency in comparison to the wild type (Figure 2B). A decreased mean recombination frequency was also determined for the uvrB deficient mutant, however, the difference between the uvrB mutant and wild type did not reach statistical significance (BF =14, “strong evidence”). There was no significant difference between the recombination frequency of the uvrC mutant and the wild type (Figure 2B). The introduction of an intact copy of the uvrA gene into the uvrA mutant restored the recombination frequency to wild type levels. In contrast, the uvrD deletion mutant (ΔuvrD) showed a hyper-recombinational phenotype (Figure 2B) that is in agreement with previous studies in E. coli and in H. pylori.
Characterization of the donor DNA imports after recombination in NER-deficient mutants
One of the characteristics of H. pylori is the import of relatively short fragments of donor DNA into the recipient chromosome after natural transformation. In order to understand whether components of the NER system play a role in the control of the length of DNA fragments replaced after natural transformation, and in the formation of interspersed sequences of the recipient (ISR), single recombination events were further characterized. For this, Rif resistant clones obtained using the in vitro transformation assay were randomly selected and a 1663 bp fragment in the rpoB locus was sequenced. Recombinant nucleotide sequences were aligned with both donor and recipient sequences to identify the different import parameters used for graphic comparisons of the polymorphisms (Figure 3). Maximum likelihood estimations (MLE) of the import size were calculated and the total number of ISR found among the isolates was counted. Statistical significance of the results was evaluated using a Bayesian approach (see Methods). Since the uvrA mutant showed a strongly reduced recombination frequency, an allele-specific PCR was used in a pre-screening step to distinguish between spontaneous mutants and recombinant clones.
There was no significant change of the import length in the uvrA, uvrB, and ΔuvrD mutants. Strikingly, the inactivation of uvrC had a strong and highly significant effect on the length of imports of donor DNA into the recipient H. pylori genome (Figure 3; Table 1). Indeed, the MLE of the imports increased more than 2-fold in the uvrC mutant compared to the wild type strain 26695 (3766 bp vs. 1681 bp, respectively). A functional complementation of this mutant restored this phenotype to wild type values, confirming that the generation of long imports was due to the absence of uvrC. None of the four mutants showed a significant change in the frequency of ISR (Table 1).
The nucleotide excision repair (NER) is a mechanism by which DNA lesions causing distortions of the helical structure (“bulky lesions”, induced by a variety of chemical agents and ultraviolet light) can be repaired. In E. coli, NER also acts on non-bulky lesions such as oxidized or methylated bases, suggesting overlapping activities of the BER and NER systems for some substrates [27, 28]. The H. pylori genome contains orthologs of all four NER genes, uvrA-D (Additional file 3: Figure S3), however the function of most of these genes, and their involvement in the unusual genetic variability of this pathogen were poorly characterized. Our data show that inactivation of each of the four H. pylori NER genes strongly increased UV sensitivity, confirming that they are indeed functional homologs of the E. coli NER genes [29, 30].
Inactivation of H. pylori uvrA and uvrB resulted in a significant reduction of the mutation frequency in comparison to the wild type strain. These results seem surprising, considering that one key function of the NER system is to limit mutations by repairing DNA lesions. Our results are, however, consistent with previous findings in E. coli, where decreased mutation frequencies were reported in uvrA and uvrB mutants after treatment with oxidized deoxyribonucleotides, while mutation rates were unaffected in a uvrC mutant . Under non-damage-inducing conditions, E. coli mutants in uvrA uvrB and uvrC exhibited a lower mutation rate . The excision and replacement of undamaged bases were first characterized by Branum and colleagues who showed that in E. coli and in human cells, NER is able to excise damage-free fragments in lengths of 12–13 and 24–32 bp, respectively . This process has been referred to as “gratuitous mutations” and it has been suggested that it may be a major source of oncogene mutations in humans [15, 33]. Such a double functionality of the NER proteins has been also reported for Pseudomonas putida and E. coli where the NER system is also involved in the generation of mutations [24, 34]. Based on our results, we hypothesize that the basal level of NER-mediated replacement activity on undamaged DNA is contributing to the overall high mutation frequency that is characteristic of H. pylori and contributes to its rapid genetic diversification [4, 7, 10]. As outlined above, the effects of uvrC inactivation on mutation rates in other bacterial species are complex and depend on the experimental conditions. We note that uvrC does not appear to contribute to the generation of gratuitous mutations in H. pylori.
The NER system has a dual role in the control of the homologous recombination in H. pylori
Our data show that the inactivation of uvrA significantly decreased the recombination frequency after natural transformation of H. pylori. A decrease was also observed with a uvrB mutant, which was suggestive (BF = 14), but did not reach statistical significance. The recombination frequency could be restored by functional complementation, indicating that UvrA facilitates homologous recombination in H. pylori. UvrA was not essential for this process, since recombinants were still detected in the mutant. Recombination frequencies differed significantly between uvrA and uvrB mutants, the reason of this statistically highly significant difference between both mutants remains to be elucidated.
Inactivation of UvrC likewise had no significant effect on recombination frequencies in H. pylori. By contrast, UvrD was found to act as an inhibitor of homologous recombination, as previously shown by other investigators .
We note that inactivation of uvrC promoted the incorporation of significantly longer DNA fragments into the H. pylori genome (2.2 fold increase) in comparison to the wild type strain, while a complemented mutant strain exhibited imports indistinguishable from wild type. We also observed that a different uvrD mutant strain, constructed by insertion of an antibiotic resistance cassette into the middle of the uvrD gene (and hence potentially capable of expressing a truncated UvrD protein), exhibited a strongly increased import length (data not shown). The mechanisms underlying these observations are as yet unclear.
Based on the data from our genetic analysis, we propose a model for homologous recombination in H. pylori (Figure 4), where DNA molecules enter the cytoplasm as ssDNAs, which are highly recombinogenic substrates [35, 36], and are loaded with RecA as nucleoprotein filaments . Thereafter, RecA catalyzes the duplex invasion whenever homology regions are encountered within the genomic H. pylori recipient strain . This results in DNA distortions that are recognized by the UvrAB complex. It remains unclear how strand breaks are introduced after this recognition, since the data indicate that UvrC is either not involved in this process, or can be functionally replaced by a different enzyme with partly redundant function. The helicase UvrD catalyzes the removal of the incised fragment and the unwinding of the DNA. Finally, the incised region will then be repaired by DNA polymerase I and ligase. UvrD also works as an anti-recombinase, by dismantling the RecA-ssDNA complex and thus leading to the restoration of the template, as found previously in E. coli and suggested for H. pylori[23, 26].
Our study provides evidence for a dual role of the NER system in H. pylori: besides its function in safeguarding genome integrity from DNA-damaging agents, it also contributes to its genetic diversity. This is accomplished first by the generation of spontaneous mutations, and second, by controlling import frequency and import length of donor DNA via homologous recombination. Even though the importance of recombination in the genetic variability of H. pylori has been well characterized, less is known about the molecular mechanisms and the regulation of the DNA incorporation. Therefore, the investigation of the NER system in homologous recombination and the specific role of UvrC in the regulation of import length are of interest for future studies. Since the gastric habitat of H. pylori is likely to be rich in DNA damaging agents, it will be of interest to study the roles of NER components in H. pylori genetic diversification under in vivo conditions, e.g. in suitable animal models. Finally, the results show the functional versatility of apparently conserved housekeeping proteins such as the NER components, emphasizing the importance of comparative functional analyses in diverse organisms, such as other naturally competent and recombining bacteria.
Bacterial strains and culture conditions
Bacterial strains used in this study are listed in Additional file 4: Table S1. H. pylori wild type strains 26695  and J99  were cultured from frozen stocks on blood agar plates (Blood agar base II, Oxoid, Wesel, Germany) containing 10% horse blood and a mix of antibiotics (vancomycin [10 mg/l], polymyxin B [3.2 mg/l], amphotericin B [4 mg/l], and trimethoprim [5 mg/l]). The agar plates were kept in an incubator with 5% O2, 10% CO2 and 85% N2 at 37°C for 24–48 h. Mutant strains were cultivated on blood agar plates containing kanamycin (20 μg/ml), chloramphenicol (20 μg/ml), or both antibiotics as required. Liquid cultures were grown in brain heart infusion (BHI, Oxoid) medium with yeast extract (2.5 g/l), 10% heat inactivated horse serum and an antibiotics cocktail (see above) in microaerobic atmosphere using air-tight jars (Oxoid) and Anaerocult® C gas generating bags (Merck).
For the DNA cloning experiments, we used E. coli strains DH5α  and MC1061 . These strains were grown in LB broth or on LB plates (Lennox L Broth, Invitrogen GmbH, Karlsruhe, Germany) supplemented with ampicillin (200 μg/ml), chloramphenicol (20 μg/ml) and/or kanamycin (20 μg/ml) as required.
All standard procedures (cloning, DNA amplification, purification and manipulation) were performed according to standard protocols . Total genomic bacterial DNA was prepared using the QIAamp DNA Minikit (QIAGEN, Hilden, Germany). Large-scale purification of bacterial chromosomal DNA was performed using QIAGEN Genomic-tip 100/G columns according to the manufacturer’s instructions. Plasmid DNA from E. coli strains was isolated using QIAGEN tip 100 columns.
Insertion mutagenesis in H. pylori
The construction of uvrA uvrB uvrC and uvrD mutants by natural transformation-mediated allelic exchange was performed as described previously . A list of the oligonucleotides used for mutagenesis, including the introduced restriction sites is provided in Additional file 4: Table S2. Briefly, the target genes were amplified by PCR and cloned into pUC18. The resulting plasmids (Additional file 4: Table S3) were used for inverse PCR amplification. Inverse PCR reactions were designed to result in the deletion of a part of the target gene (uvrA uvrB uvrC) or the complete gene (uvrD), and to introduce a unique BglII (or PstI for the uvrD construct) restriction site. The PCR products were subsequently digested with BglII (or PstI), and ligated with a kanamycin or chloramphenicol resistance cassette (aphA-3 or cat; [43, 44] flanked by the compatible BamHI (or BglII) restriction sites. The direction of transcription of the antibiotic resistance genes (kanamycin [Km] and chloramphenicol [Cm]) was the same as that of the target gene to avoid possible polar effects.
Plasmids containing the interrupted gene were used as suicide plasmids for natural transformations of the H. pylori strain 26695. The successful chromosomal replacement of the target gene with the disrupted gene construct via allelic exchange (double crossover) was checked by PCR using suitable primer combinations.
Functional complementation of mutants
Functional complementation experiments for the uvrB and uvrC mutant strains were performed by inserting an intact copy of the target gene into the ureAB locus (Additional file 4: Table S3). To do so, the ORFs HP1114 and HP0821 were cloned in the pADC vector  downstream of the strong ureAB promoter, creating the plasmids pSUS2646 and pSUS2644 (Additional file 4: Table S2 and S3). Functional complementation of uvrA was performed by inserting an intact copy of the uvrA gene together with 400 bp of DNA upstream of the start codon containing the putative uvrA promoter into the rdxA locus. The ORF HP0705 plus the upstream region were cloned in the pCJ535 vector, creating the plasmid pSUS3009. These suicide plasmids were introduced via natural transformation into the single gene mutant strains 26695 uvrA, 26695 uvrB, and 26695 uvrC, and the transformants were selected on Km/Cm blood agar plates. The correct insertion of the complementing genes in the ureAB or rdxA locus was controlled by PCR and sequence analysis of the insertion sites.
In vitro transformation system of H. pylori, determination of mutation and recombination frequencies and import sizes
The transformation system used to quantitate, in parallel, mutation and recombination rates as well as the length of the DNA fragments incorporated into the chromosome after recombination has been described previously . Mutation rates obtained with this system have been shown to be in excellent agreement with fluctuation analysis . From each experiment, at least 16 clones were expanded in order to sequence a fragment (1663 bp) of the rpoB gene (see below). The experiments were reproduced three times for each H. pylori mutant strain. To determine the length of import events in the rpoB gene, a 2361 bp PCR fragment of rpoB was amplified with primers HPrpoB-1 and HPrpoB-6 as previously described  and Additional file 4: Table S2). This PCR product was used as template for the sequencing reactions with the primers HPrpoB-3, -4, -5, -6, -9w, and −10. The six sequences from each rifampicin resistant clone were assembled using the software Bionumerics V 4.5 (Applied Maths, Sint-Martens-Latem, Belgium), yielding a continuous, double-stranded 1663 bp fragment of rpoB that included the Rif resistance-mediating point mutation of the donor strain.
PCR-based prescreening for clones with DNA imports in strain 26695 uvrA
Due to the low recombination frequency in 26695 uvrA, it was necessary to screen the Rif resistant clones after transformation in order to distinguish recombinants from spontaneous mutants. This was accomplished by allele-specific PCR using the primers HPrpoB-IscrX and HPrpoB-4, which specifically detect the Rif resistance mediating point mutation in strain J99-R3 [12, 46]. PCR positive clones were used for sequencing as described above.
UV irradiation of mutant strains
Bacteria were cultured on blood agar plates for 24 h as described above. Cells were then suspended in phosphate buffered saline (PBS) and appropriate dilutions to obtain ~100, 500 and 1,000 colonies were plated on blood agar plates in two triplicate batches. As a control, the first batch was not exposed to UV light to obtain the total cell number. The plates of the second batch were placed under a UV-C lamp (OSRAM HNS 30 W OFR, wavelength 254 nm) for two seconds at a distance of 40 cm, corresponding to approximately 100 J/m2. All plates were incubated for 72 h as previously described, colonies were counted and the percentage of surviving cells was calculated.
Growth properties of H. pylori strains
Growth curves were monitored in liquid cultures (BHI broth including 10% horse serum and antibiotics). Strains were grown for <24 h on blood agar plates and then harvested in BHI broth. The OD600 of the suspension was measured and diluted to a starting concentration of 2.1 × 107 bacteria/ml. Cultures were then incubated at 37°C in a rotary shaker (175 rpm) under microaerobic conditions. The optical density was measured at regular intervals.
Statistical analysis was performed using Bayesian model comparison, where two competing hypotheses are weighted against each other by computing the ratio of probabilities of the observed data under the two hypotheses. This ratio is called a Bayes Factor (see refs. [47, 48] for reviews). A benefit of this approach is that it accounts for the relative complexity of the hypotheses, so that the more complex one is validated only if the data justifies it. Interpretation of the Bayes Factor was done following the scale of Jeffreys : Negative (<1); Barely worth mentioning (1–3); Substantial (3–10); Strong (10–30); Very strong (30–100); Decisive (>100).
where l1 and l2 are the maximized value of the log-likelihood under the two models, k1 and k2 the number of parameters in the two models, and n the number of observations. Comparisons of frequency data between any two recipient/donor combinations were done using the BIC with one hypothesis being that the data from the two combinations comes from the same Normal distribution and the other hypothesis being that they come from two distinct Normal distributions.
Recombination start or end point were not observed exactly, but instead in an interval [mi;Mi] (with Mi = ∞ if the beginning or end is out of the sequenced region). We assume a geometric length of recombination with mean δ on both sides of the mutation conferring resistance to rifampicin. Model comparisons using the BIC found no evidence for a difference between the lengths on the two sides, and no support for a more complex negative binomial distribution which has an additional parameter compared to the geometric distribution. The likelihood of N observations is therefore equal to:
The effect of gene knock-outs on the lengths of import was evaluated using the BIC where one hypothesis is that δ remains the same and the other hypothesis is that δ changes.
Let p denote the probability of occurrence of ISR in a clone. The number m of clones containing ISR amongst n clones is thus distributed as Binomial(n,p). A Jeffrey's prior was assumed on p (i.e. Beta (½,½)). We assessed whether the probability of ISR was identical between two recipient/donor combinations (m1,n1 and m2,n2) using the Bayes Factor:
where B(.,.) denotes the Euler Beta function.
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The authors thank Christine Josenhans for plasmid pCJ535 and valuable discussions, Martin Blaser for plasmid pUvrDKm and Kerstin Ellrott, Jessika Schulze, Birgit Brenneke and Friederike Kops for excellent technical assistance. This work was supported by funding under the Sixth Research Framework Programme of the European Union, project INCA (LSHC-CT-2005-018704) and by grant SFB 900/A1 from the German Research Foundation. C.M. received a Ph.D. stipend from the German Academic Exchange Service (DAAD) and the Wilhelm Hirte Foundation. S.K. and J.K. received Ph.D. stipends from the German Research Foundation (DFG) within the frameworks of GRK 745 and IRTG 1273, respectively, as well as support through the Hannover Biomedical Research School (HBRS). Publication charges for this article were supported by the German Research Foundation in the framework of the program “Open Access Publishing”.
The authors declare to have no competing interest.
CM, JK, SK, CK, CB and SS designed the research, CM, JK, SK, CK and CB performed the experiments. XD performed all statistical analyses. CM, JK, XD, CB and SS wrote the paper. All authors analyzed data and saw and approved the paper.
Claudia Moccia, Juliane Krebes, Stefan Kulick contributed equally to this work.
Electronic supplementary material
Additional file 1: Growth curves (OD600) of H. pylori strains 26695, 26695uvrA, 26695uvrB, 26695uvrC, 26695uvrD and complemented mutant strains. (PDF 24 KB)
Additional file 2: Nucleotide sequence alignment of the 1663 bp fragment of the rpoB gene used to determine import length. Sequences are shown for strains 26695, J99 and J99R3. The sequences were aligned using CLC Sequence Viewer v6.6.1 and the point mutation (A1618T) that confers Rif resistance is labeled. (PDF 219 KB)
Additional file 3: Amino acid sequence alignments of the four NER components, UvrA, UvrB, UvrC and UvrD. The primary sequences from H. pylori 26695, C. jejuni NCTC11168, E. coli K12 and S. aureus N315 were aligned by performing a muscle alignment  using CLC Sequence Viewer v6.6.1. A. UvrA (H. pylori 26695 HP0705, C. jejuni NCTC11168 Cj0342c, E. coli K12 EG11061 and S. aureus N315 SA0714). B. UvrB (H. pylori 26695 HP1114, C. jejuni NCTC11168 Cj0680c, E. coli K12 EG11062 and S. aureus N315 SA0713. C. UvrC (H. pylori 26695 HP0821, C. jejuni NCTC11168 Cj1246c, E. coli K12 EG11063 and S. aureus N315 SA0993). D. UvrD (H. pylori 26695 HP1478, C. jejuni NCTC11168 Cj1101, E. coli K12 EG11064 and S. aureus N315 SA1721). The UvrD equivalent protein in Gram positive bacteria is known as PcrA. Amino acids conserved in three or all four orthologs are labelled with light or dark blue shading, respectively. (PDF 842 KB)
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Moccia, C., Krebes, J., Kulick, S. et al. The nucleotide excision repair (NER) system of Helicobacter pylori: Role in mutation prevention and chromosomal import patterns after natural transformation. BMC Microbiol 12, 67 (2012). https://doi.org/10.1186/1471-2180-12-67
- Helicobacter pylori
- Nucleotide excision repair