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Figure 3 | BMC Microbiology

Figure 3

From: The nucleotide excision repair (NER) system of Helicobacter pylori: Role in mutation prevention and chromosomal import patterns after natural transformation

Figure 3

Import patterns after transformation of recipient strain 26695 wild type (wt, left panel) and  uvrC  mutant (right panel) with DNA of Rif resistant strain J99-R3. Each row represents a 1663 bp partial rpoB sequence. The blue row on top represents the sequence of the recipient strain 26695, and the red row at the bottom that of the donor strain J99-R3. Vertical yellow lines represent the positions of polymorphic sites, the green line depicts the position of the point mutation that is responsible for Rif resistance in J99-R3. Numbers below the panel: position relative to the Rif resistance point mutation, negative values indicate upstream nucleotides. The rows between 26695 and J99-R3 depict 30 sequences randomly selected from 92 clones sequenced for the wt, and all 28 uvrC clones analyzed for import length. Any fragment surrounded by two sites identical to the donor is shown in red, any fragment surrounded by two sites identical to the recipient is shown in blue, and the remainder of the sequence is in white. Consequently, each sequence is shown as a mosaic of colors, where blue indicates DNA from the recipient, red DNA from the donor, and white DNA of unresolved origin.

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