Differential proteomics and physiology of Pseudomonas putida KT2440 under filament-inducing conditions
© Crabbé et al.; licensee BioMed Central Ltd. 2012
Received: 22 June 2012
Accepted: 16 November 2012
Published: 27 November 2012
Pseudomonas putida exerts a filamentous phenotype in response to environmental stress conditions that are encountered during its natural life cycle. This study assessed whether P. putida filamentation could confer survival advantages. Filamentation of P. putida was induced through culturing at low shaking speed and was compared to culturing in high shaking speed conditions, after which whole proteomic analysis and stress exposure assays were performed.
P. putida grown in filament-inducing conditions showed increased resistance to heat and saline stressors compared to non-filamented cultures. Proteomic analysis showed a significant metabolic change and a pronounced induction of the heat shock protein IbpA and recombinase RecA in filament-inducing conditions. Our data further indicated that the associated heat shock resistance, but not filamentation, was dependent of RecA.
This study provides insights into the altered metabolism of P. putida in filament-inducing conditions, and indicates that the formation of filaments could potentially be utilized by P. putida as a survival strategy in its hostile, recurrently changing habitat.
The soil bacterium Pseudomonas putida has to cope with diverse and variable habitat-associated stressors to ensure its survival . Besides the exposure of P. putida to toxic pollutants and antibacterial compounds in soils, this bacterium encounters osmotic, thermal, oxidative and starvation stresses in the natural habitat [2–5].
Under certain laboratory growth conditions, P. putida exerts a filamented phenotype . Filamentation occurs due to the lack of septation during the cell growth process and results in the formation of elongated bacteria, which is typically a consequence of DNA damage or envelope stress . Cell division inhibition is most commonly mediated by the DNA-damage response system (SOS response) . DNA damage (for example, due to ultraviolet irradiation or oxidative radicals) results in the exposure of single-stranded DNA stretches that become covered by the RecA recombinase. In this nucleoprotein filament, RecA becomes activated and stimulates the autoproteolysis of the LexA repressor, which in turn results in derepression of the SOS regulon. While most of the SOS genes are involved in DNA-repair, some carry out other functions, such as the inhibition of cell division. In this context, SulA (which is regulated by LexA) physically inhibits FtsZ polymerization and causes the formation of non-septated bacterial filaments, in order to prevent transmission of damaged DNA to daughter cells. In absence of SOS induction, however, direct chemical inhibition of FtsZ can also lead to bacterial elongation .
While reports describing conditions that induce P. putida filamentation are scarce, filamentation of other bacteria has been shown in response to DNA damage (as described above), nutrient deprivation, low temperature, media composition, low shaking speed and high osmolarity [6, 9–11]. Additionally, the different stages of biofilm development in P. putida have been associated with alterations in bacterial length . Furthermore, the plant-produced alkaloid berberine was found recently to induce filamentation in Escherichia coli K12 . Collectively, these studies indicate that conditions and/or products encountered by P. putida during its natural life cycle could induce filamentation.
For a variety of (opportunistic) pathogens, the filamentous morphology has been shown to provide survival advantages . More specifically, uropathogenic Escherichia coli (UPEC) filaments were more proficient in evading neutrophil phagocytosis compared to non-filamented UPEC . UPEC filamentation was presumably induced in response to effectors of the host innate immunity. The intracellular survival of Salmonella enterica serovar Typhimurium in macrophages in vitro is also associated with a filamentous phenotype, which is probably induced by macrophage production of nitric oxide radicals . In addition, filamentation has been shown to play a role in the infection process of, among others, Proteus mirabilis, Legionella pneumophila, Mycobacterium tuberculosis and Shigella flexneri.
It remains unclear which mechanisms are at the origin of P. putida filamentation, which metabolic changes occur in P. putida filaments, and whether the P. putida filamented phenotype could confer environmentally advantageous traits. This study is the first to assess the global proteome and stress resistance of P. putida KT2440 when grown in conditions that induce filamentation.
Morphologic and growth analysis of P. putidaKT2440 grown in filament and non-filament inducing conditions
Stress resistance of P. putidaKT2440 grown in filament and non-filament inducing conditions
Proteomic analysis of P. putidaKT2440 grown in filament and non-filament inducing conditions
Comparative proteome profile of P. putida grown at 50 rpm and 150 rpm
Up-regulated proteins (50 rpm/150 rpm)
Outer membrane porin
Outer membrane porin
50S ribosomal protein L24
Ubiquinol oxidase subunit 2
Cold-shock domain-contain protein
DEAD-box ATP dependent DNA helicase
Heat shock protein Hsp20
50S ribosomal protein L20
Magnesium-translocating P-type ATPase
Phosphate ABC transporter, periplasmic phosphate-binding protein
ATP-dependent metalloprotease FtsH
Serine-type D-Ala-D-Ala carboxypeptidase
Phosphate ABC transporter phosphate-binding protein
Ribosomal protein S3
Down-regulated proteins (50 rpm/150 rpm)
Hypothetical protein PP_0258
Glycine betaine/L-proline ABC transporter, periplasmic binding protein
Rieske (2Fe-2S) domain protein
Formaldehyde dehydrogenase, glutathione-independent
Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase
Hypothetical protein PP_0395
Putative serine protein kinase, PrkA
Aldehyde dehydrogenase family protein
Hypothetical protein PP_0765
Sigma 54 modulation protein/ribosomal protein S30EA
Sugar ABC transporter, periplasmic sugar-binding protein
Anti-oxidant AhpCTSA family protein
OmpA/MotB domain protein
DNA-binding stress protein, putative
NADH:flavin oxidoreductase/NADH oxidase
Hypothetical protein PP_1487
Dehydrogenase subunit, putative
Substrate-binding region of ABC-type glycine betaine transport system
OsmC family protein
Hypothetical protein PP_2006
Hypothetical protein PP_2105
Hypothetical protein PP_2140
Histone family protein DNA-binding protein
Hypothetical protein PP_3089
Hypothetical protein PP_3611
Transcriptional regulator MvaT, P16 subunit, putative
Isocitrate dehydrogenase, NADP-dependent
Cationic amino acid ABC transporter, periplasmic binding protein
Hypothetical protein PP_4593
DnaJ family curved-DNA-binding protein
Poly(hydroxyalkanoate) granule-associated protein
Isoprenoid biosynthesis protein
Hypothetical protein PP_5232
Aldehyde dehydrogenase family protein
Hypothetical protein PP_5260
Role of RecA in P. putidaKT2440 filamentation and stress resistance
The increased abundance of RecA (PP_1629, 2.35-fold) in 50 rpm cultures of P. putida KT2440 (Table 1) suggested the activation of the SOS response. Since only induction of RecA was observed, this could indicate a mild SOS response . In addition, the heterogeneity of the SOS response at single cell level could be masked at the population level . This heterogeneity was also apparent in cell morphology between 50 rpm- and 150 rpm-grown P. putida KT2440 (Figure 1). In order to determine whether 50 rpm-induced filamentation in P. putida KT2440 was indeed dependent on RecA, an isogenic recA mutant cultured in 50 and 150 rpm conditions was examined. Intriguingly, the 50 rpm-grown P. putida KT2440 recA mutant filamented at similar levels as the wild type P. putida KT2440 (Additional file 1: Figure S1). In contrast to filamentation, the increased heat shock resistance of P. putida KT2440 grown at 50 rpm was RecA-dependent, since an isogenic recA mutant was equally resistant to heat shock when grown at 50 rpm or 150 rpm (Additional file 2: Figure S2).
As a soil organism, P. putida recurrently encounters filament-inducing conditions during its natural life cycle. Our data indicate that filament formation of P. putida could confer environmentally advantageous traits. Indeed, P. putida KT2440 grown at low shaking speed produced filaments and was more resistant to heat shock and saline stress. Similar observations were made for Caulobacter crescentus filaments, which showed a higher resistance to oxidative, osmotic, thermal and acid stress .
The comparative proteome profile indicated that the metabolic activity of P. putida KT2440 grown at 50 rpm was significantly different from P. putida KT2440 grown at 150 rpm. The most pronounced induction occurred for the heat shock protein IbpA. This small heat shock protein belongs to the widely conserved family of α-crystallin-type heat shock proteins. The latter appears to play a very versatile role in the protection against different stress conditions via protein and membrane protection . In addition, many small heat shock proteins form oligomers, which may vary by the degree of phosphorylation or ion concentration  (induction of PP_2645, PP_2656 and PP_5329).
Although no observable differences in dissolved oxygen levels could be reported at the time of proteomic analysis (i.e., 15 hours, below detection limit for both conditions) (Figure 2), this does not completely rule out the role of dissolved oxygen in the observed results as the maximum oxygen transfer rate at 150 rpm is approximately 2.5 times higher than at 50 rpm . Ohr, a protein of the OsmC family (osmotically inducible protein) was 6.25-fold down-regulated in filamented P. putida, and is involved in the resistance to oxidative stressors, such as organic peroxide, but not in osmotic stress resistance . In addition to a decreased Ohr abundance, other proteins involved in oxidative stress resistance were present at lower levels in 50 rpm samples, including a catalase/peroxidase (PP_3668, 0.28-fold), an antioxidant AhpC (PP_1084, 0.42-fold), a glutaredoxin-related protein (PP_1081, 0.44 fold) and a putative DNA binding stress protein (PP_1210, 0.32-fold). The latter has recently been described as an oxidative stress-inducible Dps miniferritin [22, 23], and was found up-regulated in an OxyR mutant of P. aeruginosa. The differential abundance of proteins involved in oxidative stress resistance could potentially be explained by lower oxygen levels in 50 rpm cultures (and/or decreased catabolism). The increase of OprE (PP_0234, 2.41-fold) and CyoA (PP_0812, 1.82-fold) further suggests limitations in oxygen availability in 50 rpm cultures [24, 25]. Finally, oxygen limitation is related to bacterial filamentation and/or RecA induction [6, 26–28]. However, Jenssen and colleagues determined that, in addition to oxygen deprivation, additional factors such as exhaustion of medium components and changes in growth rate, were important for P. putida filamentation .
While RecA was more abundant in P. putida KT2440 grown at 50 rpm, the P. putida KT2440 recA mutant filamented at similar levels as the wild type. A similar observation was reported previously, showing that an E. coli recA mutant displayed similar levels of filamentation as the wild type strain in response to growth at high pressure, despite strong evidence of RecA-mediated SOS response activation [29–31]. Gottesman et al. (1981) suggested the existence of a transient filamentation phenotype in response to UV, independent of SulA , which could explain the RecA-independent filamentation phenotype of 50 rpm-grown P. putida KT2440 in the present study.
While the bacterial SOS response and associated filamentation is typically triggered by treatments directly affecting DNA integrity (e.g. exposure to mitomycin C or UV), a number of environmental conditions were reported to cause DNA damage in an indirect manner (e.g. starvation, aging, β-lactam antibiotics and high pressure stress) [30, 33–36]. As such, high pressure-induced filamentation of E. coli was shown to stem from the activation of a cryptic Type IV restriction endonuclease (i.e. Mrr) endogenously present in the cell , while β-lactam antibiotics triggered DpiA to interfere with DNA replication [30, 36]. Even though it remains unclear which metabolic changes could indirectly lead to DNA damage and SOS response activation, the major changes in metabolism provide evidence for new triggers of the SOS response.
In conclusion, our data indicate that filament-formation of P. putida KT2440 could confer environmentally advantageous traits, by increasing its resistance to saline and heat shock. We demonstrated that culturing at low shaking speed induced expression of RecA, which plays a central role in the SOS response, putatively through changes in amino acid metabolism and/or oxygen availability. Furthermore, the increased heat shock resistance was found to be RecA dependent. Filamentation could thus represent an adaptive survival strategy of P. putida, allowing it to persist during times of elevated soil temperatures, increased osmolarity (e.g., due to soil water evaporation) and/or increased pollution.
Bacterial strains, media and growth conditions
P. putida KT2440 (ATCC 12633) and its isogenic recA mutant derivative (kindly provided by Juan-Luis Ramos) were used in the present study. The bacterial strains were grown in Luria Bertani (LB) medium at 30°C. For incubation at different shaking speeds, an overnight shaking culture (150 rpm) of P. putida was diluted 100x in fresh LB medium. Ten milliliters of the dilution were transferred into 50 ml Erlenmeyer flasks. The flasks were placed on an orbital shaker at 50 rpm (filament-inducing condition) or at 150 rpm (non-filament-inducing condition) . Growth was monitored by measuring optical density at the 600 nm (OD600).
Analysis of dissolved oxygen levels
The measurement of the dissolved oxygen (DO) concentration of 50 and 150 rpm cultures was performed using a Knick KNI913 oxygen meter. DO levels were measured during culture, at 15 min intervals for 24 hours.
Environmental stress assays
The assessment of cell viability following exposure to saline, acid and thermal stress was performed on P. putida KT2440 grown at 50 and 150 rpm for 15 hours as described previously . The concentrations of each stress agent were as follows: 5% NaCl for osmotic stress and 10-4 M citric acid for acid stress resistance (pH = 5). For heat shock, exposure of cultures to a temperature of 55°C was applied. Cells were exposed to each stress for 30 minutes. Bacteria were diluted and plated on LB agar before and after exposure to the stress factors in order to determine the survival percentage.
The morphology of P. putida KT2440 following incubation at different shaking speeds was visualized by fluorescence microscopy of Hoechst stained cells. Briefly, 600 μl of bacterial culture (after 15 hours of growth) was resuspended in 500 μl 70% ethanol to fix the cells, incubated at room temperature for 20 min and resuspended in saline solution. Next, 2.5 μl Hoechst solution (200 μg/ml) (Hoechst 33258, Sigma-Aldrich, Belgium) was added and incubated for 20 min. Five microliters of this suspension was transferred to a microscopic glass slide, covered with a coverslip and analyzed with a Zeiss Axiovert 100M fluorescence microscope (350 nm filter, 100x oil objective). Acquisition of images was performed with an Axiocam and further processed using the Axiovision software package.
Flow cytometry analysis
P. putida KT2440 grown at different shaking speeds was analyzed with an Accuri C6 flow cytometer (Accuri Cytometers) to assess the average cell length. Forward and side scatter signals were measured and a total of at least 10,000 cells were recorded for each sample. The respective cell populations were delimited to eliminate background signals originating from cell debris. All data analysis was performed with the CFlow Software.
Protein extraction and analysis was performed on P. putida grown at 50 and 150 rpm for 15 hours. Proteins were extracted and labeled isotopically using ICPL, and the post-digest procedure was performed as described in . Labeled tryptic peptides were submitted to online 2D-LC separation prior to MS/MS analysis as described previously , except that SCX column was eluted with 11 plugs of increasing NH4Cl concentration (5, 10, 25, 50, 75, 100, 125, 150, 200, 400 and 800 mM in loading solvent).
For MS/MS data processing, peptide peaks were detected and processed using Mascot Distiller (version 2.3.2). Created peak list was used as the input for Mascot MS/MS Ions searches using an in-house Mascot 2.2 server (Matrix Science) against the NCBInr database restricted to Pseudomonas putida (KT2440). The search parameters used were: enzyme = trypsin; Max. Missed cleavages = 2; Fixed modifications = Carbamidomethyl (C); Variable modifications = Oxidation (M); ICPL modification at both peptide N-ter and lysine side chain. Peptide tolerance ± 1.3 Da; MS/MS tolerance ± 0.5 Da; Peptide charge = 2+ and 3+; Instrument = ESI-TRAP. Only proteins identified with a protein score above the calculated Mascot ion score, defined as the 95% confidence level, were considered.
Mascot distiller was also used for protein quantification with parameters as follows: integration method: simple; correlation threshold: 0.8; standard error threshold: 999; Xic threshold: 0.2; max Xic width: 7; fraction threshold: 0.5 and mass time matches allowed. Only peptides with an ion score above 30 were considered for quantification. The protein ratio corresponds to the average of peptide ratios. After examination that the distribution of protein ratios was almost centered on 1, a normalization based on the median of the peptide ratios was realized by mascot distiller on the complete dataset. Proteins with fold changes above 1.5 or below 0.66 were considered as in modified abundance.
All experiments were performed in triplicate, unless stated otherwise. The statistical determination of significance (α = 0.05) was calculated using a Student’s t-test on the biological replicates of each experimental condition.
This work was partially supported by the European Space Agency ESA/ESTEC through the PRODEX program in collaboration with the Belgian Science Policy through the BASE project. We thank Ilse Coninx, Wietse Heylen and Giuseppe Pani for excellent technical assistance.
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