Skip to main content
  • Research article
  • Open access
  • Published:

Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases



Efficient perception of attacking pathogens is essential for plants. Plant defense is evoked by molecules termed elicitors. Endogenous elicitors or damage-associated molecular patterns (DAMPs) originate from plant materials upon injury or pathogen activity. While there are comparably well-characterized examples for DAMPs, often oligogalacturonides (OGAs), generated by the activity of fungal pathogens, endogenous elicitors evoked by bacterial pathogens have been rarely described. In particular, the signal perception and transduction processes involved in DAMP generation are poorly characterized.


A mutant strain of the phytopathogenic bacterium Xanthomonas campestris pv. campestris deficient in exbD2, which encodes a component of its unusual elaborate TonB system, had impaired pectate lyase activity and caused no visible symptoms for defense on the non-host plant pepper (Capsicum annuum). A co-incubation of X. campestris pv. campestris with isolated cell wall material from C. annuum led to the release of compounds which induced an oxidative burst in cell suspension cultures of the non-host plant. Lipopolysaccharides and proteins were ruled out as elicitors by polymyxin B and heat treatment, respectively. After hydrolysis with trifluoroacetic acid and subsequent HPAE chromatography, the elicitor preparation contained galacturonic acid, the monosaccharide constituent of pectate. OGAs were isolated from this crude elicitor preparation by HPAEC and tested for their biological activity. While small OGAs were unable to induce an oxidative burst, the elicitor activity in cell suspension cultures of the non-host plants tobacco and pepper increased with the degree of polymerization (DP). Maximal elicitor activity was observed for DPs exceeding 8. In contrast to the X. campestris pv. campestris wild type B100, the exbD2 mutant was unable to generate elicitor activity from plant cell wall material or from pectin.


To our knowledge, this is the second report on a DAMP generated by bacterial features. The generation of the OGA elicitor is embedded in a complex exchange of signals within the framework of the plant-microbe interaction of C. annuum and X. campestris pv. campestris. The bacterial TonB-system is essential for the substrate-induced generation of extracellular pectate lyase activity. This is the first demonstration that a TonB-system is involved in bacterial trans-envelope signaling in the context of a pathogenic interaction with a plant.


Plant cells are permanently monitoring their immediate environment to identify attacking pathogens and subsequently initiate defense. Highly conserved molecular structures termed microbe-associated molecular patterns (MAMPs) or pathogen-associated molecular patterns (PAMPs) are obvious targets for this recognition process. The term PAMP-triggered immunity (PTI) is increasingly used for this innate immunity [1]. Recognition by the plant employs transmembrane pattern recognition receptors (PRRs). Unfortunately, so far there are only a few detailed model systems that describe MAMP, PRR, and perception-induced signaling [2]. An example for such a well-characterized PTI is the recognition of bacterial flagellin in Arabidopsis thaliana[3]. In older literature, molecules which evoke defense-related plant reactions and which hence are assumed to be involved in the recognition process of non-host plants were termed elicitors [2]. Plant defense upon pathogen recognition typically includes the induction of a so-called hypersensitive response (HR), which leads to the resistance of the non-host plants and which includes a rapid local generation of superoxide, the so-called oxidative burst, and a programmed cell death [4]. Examples for MAMPs are the harpin proteins from Erwinia[5, 6], Xanthomonas[7, 8], or Pseudomonas[9], syringolides from Pseudomonas syringae[10] or lipopolysaccharides (LPSs), characteristic glycoconjugate cell envelope constituents of Gram-negative bacteria [11].

In addition to monitoring for pathogen-derived MAMPs, plants recognize endogenous molecules that are released upon injury or infection as alarm signals. Such molecules are termed damage-associated molecular patterns (DAMPs) [12]. Often DAMPs are generated by lytic enzymes of attacking pathogens when they breach structural barriers of plant tissues, in particular plant cell walls. DAMPs include oligosaccharide fragments, peptides resulting from protein degradation [13], and reactive oxygen species (ROS) [14]. Plants can amplify the response to DAMPs by inducing specific enzymes that generate additional similar DAMP molecules [15]. Examples for DAMPs known for a long time are oligogalacturonides (OGAs) that are released by fungal pectate lyases [1618] from plant cell walls. Among the plant pathogenic bacteria, so far only Erwinia carotovora has been reported to induce the generation of a DAMP [19], which also turned out to be an OGA [20]. Upon the discovery of the egg box conformation of OGA dimers [21], the A. thaliana wall-associated kinase 1 (WAK1) was identified as a candidate for a PRR that specifically recognizes OGAs. While the receptor-like kinase WAK2 was shown to be involved in pectin-dependent signaling [22], a recent domain-swap experiment confirmed the identification of WAK1 as OGA receptor [23], thereby turning the plant side of OGA perception into a comparably complete model of DAMP recognition.

Xanthomonas species are members of the γ subdivision of the Gram-negative Proteobacteria, which have adopted a plant-associated and usually plant pathogenic lifestyle [24, 25]. Xanthomonas campestris pv. campestris is a pathogen of Brassicacea including A. thaliana. Upon infection of cabbage plants it causes the black rot disease. In non-host plants like pepper (Capsicum annuum) and tobacco (Nicotiana tabacum), however, it induces an HR. For X. campestris pv. campestris, LPSs [2629], as well as muropeptides [30], fragments of the bacterial cell wall material peptidoglycan, have been characterized as MAMPs. Non-host resistance of plants towards X. campestris pv. campestris seems to be a very complex situation, where multiple elicitors are active in parallel [26, 31]. The genetic analyses performed during the last years identified several gene loci that are linked to the pathogenicity of X. campestris pv. campestris in host plants and to the induction of a resistance response in non-host plants. Protein secretion systems, in particular the type III secretion system, have an important role in the pathogenic interactions with plants [3235]. Further virulence factors are exported by type II secretion systems [32, 36]. They are involved in the secretion of extracellular enzymes including plant cell wall degrading enzymes like pectate lyases (EC, also known as polygalacturonate lyases [3740], or polygalacturonases (EC [40, 41]. Pectate lyases catalyze the cleavage of α­1,4 glycosidic bonds between galacturonic acid residues of homogalacturonans. Likewise, polygalacturonases catalyze the cleavage of the glycosidic bonds between adjacent galacturonic acid residues, but the hydrolysis of the glycosidic linkage results in the addition of a water molecule from the environment. Genome data which are now available for several strains have further added to our understanding of pathogenicity loci in X. campestris[4247]. More information can be derived from closely related pathogens like Xylella fastidiosa, where a polygalacturonase has been characterized that is similar to the pglA2 gene product of X. campestris pv. campestris B100 [48]. Rapid progress is currently achieved in identifying and analyzing regulation in X. campestris[4952]. Concerning signal transduction, there has been substantial advancement of science related to two complex systems of cell-cell communication that employ a diffusible signal factor (DSF) [53] and a diffusible factor (DF) [54], respectively. In addition, more and more X. campestris two-component systems signal-transduction systems are characterized experimentally [5558].

In previous analyses, the X. campestris pv. campestris tonB gene cluster showed some very interesting characteristics. TonB systems of Gram-negative bacteria are multi-component transport systems that perform the specific active uptake of various compounds across the outer membrane [59]. These systems consist of the core components TonB, ExbB, and ExbD, which are located at or within the inner membrane, and variable so-called TonB-dependent receptors, which are located in the outer membrane, and which are specific for the imported substrate [60]. TonB is assumed to transfer energy, which is gathered by the inner membrane components ExbB and ExbD from the inner membrane’s proton motive force, to the TonB-dependent receptors in the outer membrane. The TonB system is particular known for the uptake of iron [61]. For X. campestris pv. campestris, an unusual high number of diverse TonB-dependent receptors has been identified in a profound analysis [62]. Functional data revealed, besides iron, carbohydrates as substrates imported by specific TonB-dependent receptors of X. campestris pv. campestris [62]. A gene of a TonB-dependent receptor that was co-located wtih genes for two putative pectin/polygalacturonate degrading enzymes was induced by polygalacturonate [62]. TonB-dependent receptors are part of a regulon involved in utilization of N-acetylglucosamine, but their specific role remained unclear [63]. The contiguous X. campestris pv. campestris genes tonB, exbB, and exbD1, which code for the TonB system core components, are essential for iron uptake [64]. They are also required to induce the black rot disease in Brassica oleracea, to induce an HR in the interaction with the non-host plant C. annuum, and they are involved in the infection of X. campestris pv. campestris by the lytic bacteriophage ΦL7 [65]. Differing from other Gram-negative bacteria, in X. campestris pv. campestris there is a similar second exbD gene, termed exbD2, which is located in the same gene cluster in tandem directly downstream of exbD1[64]. This gene is not essential for iron-uptake, not necessary to induce the black rot symptoms on host plants, and not essential for penetration by phage ΦL7, but it is required to induce an HR in non-host plants [66]. A similar but not identical genetic organization with two exbD genes located in tandem has only been described for the fish-pathogenic Flavobacterium psychrophilum, where again the exbD2 gene, which was also not required for iron uptake, was involved in pathogenicity [67]. Although the role of the X. campestris pv. campestris exbD2 gene is not well understood in detail, there are hints that the gene product is involved in the export of X. campestris pv. campestris exoenzymes.

In this study, we have analyzed the exbD2 gene in more detail. In the course of the analyses, we discovered that exbD2 is involved in the induction of bacterial pectate lyase activity, which then releases OGAs from plant-derived pectate that are subsequently recognized as a DAMP by the plant.


The structure of the tonB gene cluster of X. campestris pv. campestris is unusual, and the role of the second exbD gene located in this cluster is still puzzling. Differing from the genes tonB, exbB, and exbD1, exbD2 is not required for iron uptake [64], but it is essential to induce an HR on C. annuum[66]. Hence, further analyses were performed to obtain a better understanding of this enigmatic pathogenicity-related gene.

Genomic analysis of X. campestrispv. campestris TonB core genes

The availability of genome data [43, 45, 46] allowed a broader perspective on the TonB system of X. campestris pv. campestris. This led already to the discovery of an unexpected wealth of TonB-dependent receptors [62]. A detailed genomic analysis revealed now the presence of further genes coding for components of TonB systems (Figure 1A). In total, five copies of tonB, two copies of exbB and four copies of exbD were identified within the genome. Downstream of the previously characterized tonB-exbB-exbD1-exbD2 genes, which are located close to the chromosomal origin of replication, a third exbD gene was identified (Figure 1B). While the presence of different TonB-dependent receptors has been attributed to their distinct binding specificities, where different molecules are bound at the outer cell surface to be either transported inside or to signal their presence to the cell interior, so far it has been assumed that only one set of tonB-exbB-exbD genes is required to build a TonB protein complex that interacts with all the different TonB-dependent receptors. Results of previous mutational analyses [64] suggest that the newly identified genes of TonB system core components are not involved in iron uptake. To shed more light on the multiplicity of these genes, we concentrated on analyzing the function of exbD2, which had already been shown to be involved in plant interaction, despite being not important for iron uptake [66]. A genomic comparison showed that this gene was present and well conserved in all complete Xanthomonas genomes (Additional file 1).

Figure 1
figure 1

Genomic organization of the TonB-related genes in X. campestris pv. campestris B100. (A) A circular genome plot indicates the locations of the TonB-related genes on the chromosome. The core of the TonB system is encoded by the genes tonB, exbB and exbD. In X. campestris pv. campestris B100 multiple isoforms of these genes were identified. Their genomic locations on the circular chromosome are indicated. So far, this multiplicity was only known for tonB genes in Pseudomonas[68] and for the exbD genes in Flavobacterium psychrophilum, where two paralogous genes were found in tandem in a cluster combined with tonB and exbB[64] close to the chromosomal origin of replication (B). Size and direction of transcription is illustrated by arrows for this gene cluster. Genes that were predicted with convincing evidence are symbolized by shaded arrows, while an open arrow indicates a putative protein-coding sequence (CDS) that was predicted with less confidence. Now a third copy of exbD was found downstream of exbD2, separated from exbD2 only by a hypothetical gene for which nor functionality neither expression could be indicated. Further copies of tonB and the genes exbB-exbD were found at different chromosomal positions. To facilitate discriminating the individual genes, unique numbers were added to their names.

The exbD2gene is involved in pectate lyase activity

X. campestris pv. campestris is well-known for its pathogenicity-related extracellular enzymes, among them a pectate lyase [37, 38]. Mutant strains affected by insertions of ΩKm(cat) cassettes in tonB1, exbB1, exbD1, and exbD2[64] were not reduced in the activities of their extracellular amylases, proteases, and carboxymethyl cellulases, respectively, when compared to the wild-type (data not shown). However, an agar plate test for pectate lyase activity showed no activity for mutants deficient in tonB1, exbB1, exbD1, and exbD2, while there were substantial halos caused by pectate degradation around colonies of the wild-type and a positive control (Figure 2). The lost extracellular pectate lyase activity could be recovered for all mutant strains including the exbD2 mutant B100-11.03 by introducing plasmids carrying specific copies of the complete genes [64, 66]. The halos encircling the complemented mutants were only slightly less pronounced in size than halos around the wild-type strain B100 (Additional file 2). Due to the parallel presence of genes for pectate lyases and polygalacturonases in X. campestris pv. campestris B100, it is in several cases impossible to distinguish between these enzyme classes by means of phenotypic effects such as digestion of polygalacturonic acid in agar plate tests. In such cases, the term "pectate lyase" is used in a loose manner in this manuscript and meant to include polygalacturonases.

Figure 2
figure 2

Test for pectate lyase activity in TonB-related mutants of X. campestris pv. campestris. X. campestris pv. campestris wild-type strain B100 and mutants derived from it with disrupted genes coding for core components of the TonB system were grown for two days on M9 minimal medium supplemented with pectate and FeSO4. The positions of the inocula are indicated by dashed circles. Staining with Ruthenium Red unveiled halos encircling the inocula of the wild-type and a control strain that indicate activity of extracellular pectate lyases [64], while no halos were visible when the genes tonB1, exbB1, exbD1, and exbD2 were disrupted. The mutant strain B100-6.01 [64], carrying an ΩKm(cat) insertion in the non-coding region between tonB and exbB, was tested as a positive contro.

These first results were checked in a more elaborate approach. The strains B100-5.05 (tonB1), B100-7.03 (exbB1), B100-9.01 (exbD1), B100-11.03 (exbD2), and the wild-type were grown in liquid medium under inducing conditions. The pectate lyase activity was determined in a photometric assay [38]. In contrast to the wild-type, all mutant samples showed no pectate lyase activity, see Additional file 3: Table S1. As no structural genes coding for pectate lyase enzymes were affected by the X. campestris pv. campestris mutations analyzed, it seemed likely that the mutations in the genes tonB1, exbB1, exbD1, and exbD2 affected the induction of pectate lyase genes.

Pectate lyase activity is required for HR on C. annuum

Since the breakdown of pectate in plant cell walls is supposed to be an important factor in the pathogenic interaction of X. campestris pv. campestris with its host plants, the missing pectate lyase activity could be a reason for the absence of HR in the X. campestris pv. campestris mutants defective in tonB1, exbB1, exbD1, or exbD2. This hypothesis was checked in a complementation experiment. The pglI gene coding for pectate lyase isoform I had been functionally characterized based on X. campestris pv. campestris wild-type strain 8004 [38, 39]. This gene, which is orthologous to the X. campestris pv. campestris B100 gene termed pel1, was cloned from cosmid pIJ3051 [39] to finally obtain the plasmid pHGW267, where pglI was constitutively expressed under the control of the aacC1 Pout promoter (see methods section for details). This plasmid, which could not replicate in X. campestris pv. campestris, was integrated into the chromosomes of the X. campestris pv. campestris wild-type strain B100 and of the exbD2 mutant, which was not affected in iron uptake [64]. The pectate lyase of the resulting complemented strains was also active in the absence of pectate, although the activity was decreased by about 50% when compared to the pectate-induced wild-type (Additional file 3: Table S2). So these strains did not require induction for pectate lyase activity. Both X. campestris pv. campestris strains carrying the constitutively expressed pglI gene, the wild-type as well as the exbD2 mutant, were then infiltrated into C. annuum leafs. Here, the complemented exbD2 mutant induced an HR with symptoms similar to the wild-type, although with a delay of one day (Figure 3). Hence, the intensity of the HR correlated well with pectate lyase activity. The results show that X. campestris pv. campestris pectate lyase activity is required to invoke an HR on pepper.

Figure 3
figure 3

Complementation of an X. campestris pv. campestris exbD2 mutant by a constitutively expressed pglI gene from X. campestris pv. campestris 8004. When compared to the X. campestris pv. campestris wild-type strain B100, it becomes obvious that the mutant strain defective in exbD2, B100-11.03, which had been demonstrated before to induce no symptoms like necrotic lesions [66], could be functionally complemented with a constitutively expressed pglI gene on plasmid pHGW267 that was integrated into the chromosome. (A) The complemented mutant strain regained its pectate lyase activity, although not to the full extent of the wild-type strain. (B) This correlates well with the reconstituted but attenuated hypersensitive response that this complemented mutant evoked on C. annuum.

Elicitor-activity upon co-incubation of X. campestris pv. campestris with C. annuumcell wall material

The successful complementation of an exbD2 mutant with a pectate lyase gene indicated an important role of this gene in the recognition of X. campestris pv. campestris pathogens by non-host plants. However, the molecular characteristics of the elicitor that caused the HR were still unknown. The pectate lyase itself could act as a MAMP. Alternatively, products of the pectate lyase reaction like depolymerized pectate fragments derived from the plant cell wall could be recognized by the non-host plants. So, also a DAMP could not be ruled out as a possible cause of the HR.

This hypothesis was tested experimentally by co-incubating the bacteria with isolated cell wall material. Plant cell walls were prepared from C. annuum leafs detached from 6 week old plants grown in the greenhouse. The plant material was homogenized and extracted with aqueous and organic solvent systems, resulting in a crude cell wall preparation. This cell wall material was incubated for 12 h with X. campestris pv. campestris B100-Bac2 cells. The incubation was carried out in phosphate buffer to avoid interference by any additional nutrient source for the bacteria. After removing cell wall fragments and bacteria by centrifugation, the supernatant was boiled to inactivate all enzyme activity (5 min, 100°C), centrifuged again and dialyzed with a molecular-weight cut-off of 1000 Da. These samples were tested for elicitor activity in tobacco suspension cell cultures by measuring H2O2 generation, the so-called oxidative burst. While the supernatant of incubated cell walls induced no activity, the cell walls co-incubated with X. campestris pv. campestris gave rise to an oxidative burst that indicated the presence of a soluble elicitor (Figure 4A). All experiments performed to characterize the elicitor were initially carried out with plant suspension cell cultures from the non-host N. tabacum, since they are easier to handle and more responsive to elicitors than pepper cell cultures. Parallel controls containing just X. campestris pv. campestris bacteria or just cell wall material, respectively, were prepared. Unexpectedly, the X. campestris pv. campestris control caused an oxidative burst reaction with an amplitude that indicated nearly half of the activity observed in the measurement with the X. campestris pv. campestris-cell wall co-incubation. A possible explanation could be derived from previous experiments. It was shown that X. campestris pv. campestris lipopolysaccharides (LPSs) are MAMPs that induce pronounced elicitor activity [26, 69]. Since LPS is also released to the supernatant and would not be removed or inactivated by the heat treatment, these molecules could be responsible for the oxidative burst caused by the X. campestris pv. campestris supernatant. To purify the supernatants from LPS, polymyxin B agarose was employed, which binds LPS with high affinity. By this method, essentially all elicitor activity could be removed from the X. campestris pv. campestris supernatant (Figure 4B). In contrast, for the X. campestris pv. campestris cell wall co-incubation, the polymyxin B agarose treatment reduced the elicitor activity only by about 50%. Obviously, a heat-stable elicitor, differing from bacterial LPS, had remained within this sample.

Figure 4
figure 4

Oxidative burst reactions in heterologous N. tabacum cell suspension cultures after elicitation with supernatants of X. campestris pv. campestris co-incubated with plant cell wall material. The production of hydrogen peroxide was quantified by means of an H2O2-dependent chemiluminescence reaction (A). For each measurement, 200 μl of the respective supernatants were added to the cell cultures. The hydrogen peroxide formation was monitored at different time intervals upon the addition of supernatants of C. annuum cell wall material (), supernatants of X. campestris pv. campestris cultures (▲), supernatants of X. campestris pv. campestris cultures co-incubated with C. annuum cell wall material (), and for a negative control of 200 μl water (♦). There was a clear oxidative burst upon the addition of a supernatant of X. campestris pv. campestris co-incubated with cell wall material, but an almost similar explicit reaction when a supernatant of X. campestris pv. campestris was added that had not been co-incubated with cell wall material. (B) Supernatants of X. campestris pv. campestris cultures were treated with polymyxin B agarose to remove LPS. Then the effect of the purified supernatants on N. tabacum cell suspension cultures was analyzed. The formation of H2O2 was monitored upon the addition of supernatants of X. campestris pv. campestris cultures (▲), supernatants of X. campestris pv. campestris cultures co-incubated with C. annuum cell wall material (), supernatants of X. campestris pv. campestris cultures purified from LPS (■), supernatants of X. campestris pv. campestris cultures co-incubated with C. annuum cell wall material and purified from LPS (), and after adding 200 μl water as a negative control (♦). Removing the LPS reduced the response to X. campestris pv. campestris supernatant to the level of the water control. In contrast to this, the removal of LPS reduced the amplitude of the cell culture response to X. campestris pv. campestris co-incubated with cell wall material, but this supernatant still evoked a clear oxidative burst reaction.

In the X. campestris pv. campestris mutant strain B100-11.03, the exbD2 gene had been inactivated [64]. While this has no effect on iron uptake [64], the main function usually associated with the TonB import system, this mutant is affected in pathogenicity on non-host plants [66] and was now shown to lack pectate lyase activity unless complemented with a constitutively expressed pectate lyase gene. Hence, it was tempting to analyze the effect of the mutant B100-11.03 on C. annuum suspension cell cultures. While the well-known elicitor invertase and supernatant of the wild-type X. campestris pv. campestris B100 caused typical oxidative burst reactions, there was no response to the mutant B100-11.03 (Figure 5). Thus again, an involvement of the affected exbD2 gene in the production of the elicitor was obvious.

Figure 5
figure 5

Hydrogen peroxide formation in C. annuum cell suspension cultures upon elicitation with supernatant of an X. campestris pv. campestris exbD2 mutant co-incubated with cell wall material. To assess the role of the exbD2 gene in provoking defense reactions in non-host plants, cultures of the X. campestris pv. campestris mutant strain B100-11.03 were co-incubated with cell wall material from C. annuum. Then the formation of H2O2 was monitored in cell suspension cultures of C. annuum upon the addition either supernatants of X. campestris pv. campestris wild-type cultures (), supernatants of X. campestris pv. campestris cultures affected in exbD2 that were co-incubated with C. annuum cell wall material (♦), invertase as a positive control (■), or C. annuum cell wall material employed as negative control (). The mutated bacterial mutant strain deficient in exbD2 could not evoke an oxidative burst reaction.

Evidence that the newly formed elicitor is an oligogalacturonide DAMP

The isolation of the cell wall derived elicitor excluded proteins as active compound as the heating step (5 min 100°C) with subsequent centrifugation should remove or inactivate proteins from the supernatant. Considering these preliminary facts and that X. campestris pv. campestris is not known to produce pectate, the most likely candidate for an elicitor was an oligosaccharide or polysaccharide originating from enzymatic digestion of the plant cell wall. To further characterize the elicitor, the supernatant was treated with periodic acid, which is able to oxidize carbohydrates. This treatment led to a completely inactive supernatant that could not provoke oxidative bursts (data not shown). This was in good accordance with an elicitor composed of carbohydrates like oligosaccharides or polysaccharides. To further characterize the elicitor, the monosaccharide composition of the supernatant was determined by total hydrolysis with trifluoroacetic acid. The resulting monosaccharide sugars were identified by HPAEC (high-performance anion exchange chromatography; Figure 6). Glucose was particular abundant in the controls, X. campestris pv. campestris bacteria and plant cell wall supernatant, with minor amounts of galactose and rhamnose. In contrast, the co-incubation suspension of plant cell wall material and bacteria showed a different distribution of neutral sugars. Here, rhamnose and galactose were abundant while glucose was present in smaller amounts. The co-incubation contained also a small amount of mannose. The sugars abundant in the co-incubation suspension are constituents of plant cell walls. Rhamnose and galactose are for example components of hemi-celluloses.

Figure 6
figure 6

Effect of the co-incubation of X. campestris pv. campestris with plant cell wall material on the composition of the dissolved monosaccharides. The identity and relative amounts of the monosaccharides in the supernatant of X. campestris pv. campestris co-incubated with cell wall material of C. annuum was determined by HPAEC. The sugars were separated and identified using an isocratic elution with10 mM sodium hydroxide and amperometric detection on a CarboPac® PA-100 column, a set up that allows detecting the dissolved neutral sugars. The results were compared to the supernatant of an X. campestris pv. campestris culture that had had no contact to plant cell wall material, and to analogously treated cell wall material that had not been incubated with bacteria. The supernatants of plant cell wall material (A) and the X. campestris pv. campestris culture (B), which were analyzed as controls, were both mainly composed of glucose (Glc), galactose (Gal), and rhamnose (Rha). When plant cell wall material and X. campestris pv. campestris culture were co-incubated (C), the amounts of rhamnose and galactose increased dramatically, reverting the original relative abundances. In addition, small amounts of mannose (Man) became detectable.

Another major component of the plant cell wall is galacturonate, which is the building block of pectate and which in combination with rhamnose. To monitor also this compound, compositional analyses of the charged sugars were carried out using HPAE chromatography. These experiments gave evidence that the co-incubation of plant cell wall material and X. campestris pv. campestris contained more galacturonate than the controls (data not shown). As Xanthomonas has extracellular pectate lyases, it seemed reasonable that the elicitor-active compound could be a pectate fragment from the plant cell wall and hence a DAMP, as it was reported for E. carotovora[19]. The elicitor-active compound was analyzed via HPAE-chromatography to test this hypothesis (Figure 7). While no oligosaccharides were indicated for the individual supernatants of bacteria and cell walls, respectively, the co-incubation of both resulted in the formation of a distinct oligosaccharide pattern. The elution profile of these oligosaccharides from a gradient ranging from 0.01 M to 1 M sodium acetate indicated negatively charged oligosaccharides. Complementarily to the pulsed amperometric detection, UV-absorption was measured at 240 nm. The newly formed oligosaccharides exhibited UV-absorption. This criterion reasonably pointed to OGAs with an unsaturated C-C bond produced by lyase activity. As a standard, purified pectin was depolymerized by commercially obtained pectate lyase. The co-incubation showed the same elution profile as the depolymerized pectate standard, but a different quantitative distribution of the degrees of polymerization. Co-injection of the elicitor-active compounds with a pectate standard showed no differences between the two elution patterns, leading to the well-founded assumption that bacterial exoenzymes, most likely a bacterial lyase, were responsible for the release of these OGAs from the plant cell wall.

Figure 7
figure 7

HPAEC characterization of the elicitor-active compound. A sodium acetate gradient ranging at 0.1 M NaOH from 0.01 M to 1 M sodium acetate with a plateau of 10 min. at a concentration of 0.7 M facilitated the identification of oligosaccharides on a CarboPac® PA-100 column with pulsed amperometric and UV-detection. Supernatants of C. annuum cell wall material (A) and an X. campestris pv. campestris culture (B) displayed no oligosaccharide signals. However, when C. annuum cell wall material was co-incubated with an X. campestris pv. campestris culture (C), characteristic peaks were detected that eluted between 10 min and 20 min. and that indicated the formation of oligosaccharides. A pectate standard of OGAs generated by digesting commercially available pectin with pectate lyase was analyzed as a control (D). The characteristic oligosaccharide peaks of both runs (C and D) were eluted at similar retention times. When the pectate standard was mixed with co-incubation supernatant, the HPAEC analysis indicated perfect overlapping of the congruent oligosaccharide peaks (E). Hence it was plausible to identify the oligosaccharides from the co-incubation of C. annuum cell wall material and X. campestris pv. campestris culture as OGAs.

The structure of the OGA DAMP was further characterized by mass spectrometry. Upon desalting and lyophylization, the supernatant of the co-incubation of cell wall material and X. campestris pv. campestris was analyzed by MALDI-TOF MS (Figure 8). Mass fingerprints obtained in negative-ion mode displayed a ladder-like pattern with identical mass differences corresponding to the molecular weight of galacturonic acid. The analysis of the co-incubation revealed a prevalence of OGAs with degrees of polymerization (DP) around 8 (Figure 8). Combined with the results of total hydrolysis and monosaccharide identification by HPLC, this MALDI-TOF MS data strongly indicates the presence of linear OGAs within the supernatant of the co-incubation. Furthermore, a covalent carbon double bond can be assumed for the reducing end of the oligosaccharide due to the UV-absorption of these oligomers.

Figure 8
figure 8

MALDI-TOF MS of oligosaccharides released from C. annuum cell walls by co-incubation with X. campestris pv. campestris. Cell walls of C. annuum and bacteria were co-incubated over night and the cell-free supernatant was desalted and lyophilized. This material was applied to MALDI-TOF MS using the negative-ion mode. A characteristic ladder of negatively charged ions was obtained. Mass differences correspond to that of OGAs of different degrees of polymerization (DP). Ions that correspond to DP 7 to 12 are indicated.

Elicitor activity of pectate fragments in N. tabacum and C. annuumcell suspension cultures

To assess their functional roles, OGAs with different DPs were isolated. The gradient that had been employed successfully in the qualitative analyses was applied again, now with a semi-preparative column to obtain sufficient material for the subsequent characterizations (Figure 9). Pectate lyases are known to degrade pectate polymers mainly to oligosaccharides with DPs of 2, 3, and 4, while generating galacturonate monomers is uncommon these enzymes [37]. Thus the DP of the smallest detected OGA was supposed to be 2, and the peaks were numbered accordingly. Fractions containing oligosaccharides of a DP under 9 were isolated individually, while the fractions with saccharides of higher DPs were pooled. The purity of the isolates was tested by re-chromatography.

Figure 9
figure 9

Isolation of oligogalacturonides (OGAs) with varying degree of polymerization. As the chromatogram of C. annuum cell wall material co-incubated with an X. campestris pv. campestris culture was identical to OGAs derived from pectin digested with a pectate lyase, the products of the co-incubation were assumed to be OGAs, too. The activity of the X. campestris pv. campestris culture supernatant had obviously generated a diverse set of OGAs varying by their degree of polymerization (DP), with a minimal DP of 2, see main text. To allow a further characterization of the OGAs, eluted fractions representing the different individual OGAs were isolated by a sodium acetate gradient, ranging from 0.01 M to 1 M, 0.1 M NaOH with a plateau of 10 min. at a concentration of 0.7 M on a semi-preparative CarboPac® PA-1 column.

The isolated OGAs were then tested for their ability to induce oxidative burst reactions in the heterologous non-host plant cell suspension cultures of N. tabacum (Figure 10). After addition of the isolates to the cell suspension cultures, small OGAs (DP 1 to 4) had only weak elicitor activities. With increasing degree of polymerization (DP 4 to 8), the elicitor activity of the isolates rose clearly. The pooled OGAs with a DP exceeding 8 were able to induce an oxidative burst similar to that of a general elicitor like yeast extract. As this isolate was a mixture of different DPs, the concentration of the elicitor-active OGAs was well at a nanomolar level. Finally, the response of cell suspension cultures of the homologous non-host plant C. annuum to the OGA elicitor (DP > 8) was measured. The cultures showed a specific oxidative burst reaction (Figure 11). The reaction exhibited a time course with a maximum at 25 to 30 min. and an amplitude of 25 to 50 μmol H2O2, both comparable to the reaction observed for the cell cultures of the heterologous non-host plant tobacco. All these results confirmed the identification of OGAs as a DAMP generated by the activity of X. campestris pv. campestris pectate lyases.

Figure 10
figure 10

Oxidative burst reactions in heterologous N. tabacum cell suspension cultures after elicitation with isolated OGAs. To functionally characterize OGAs differing in their DPs they were checked for their capacity to evoke oxidative burst reactions in cell-suspension cultures of the non-host plant N. tabacum. Samples of the OGAs were added to the cell suspension cultures to a final concentration of 5 μg/ml. The amount of H2O2 produced upon the addition of the OGAs was monitored as described before. The addition of water used as negative control (♦) had no effect, and OGAs with a DP of 2 (■), a DP of 3 (), a DP of 4 (▲), and a DP of 6 () had only minimal effects on the N. tabacum suspension cells. The response to OGAs with DPs of 5 (), 7 (□), and 8 (∆) was slightly stronger but still small. But for OGAs whereof the DP exceeded 8 (), a clear oxidative burst reaction was observed. This indicated the largest OGA fraction as elicitor of the non-host plant defense against X. campestris pv. campestris.

Figure 11
figure 11

Oxidative burst reaction in homologous C. annuum suspension cell cultures after elicitation with OGAs of a DP exceeding 8. A fraction of isolated OGAs, which had a DP of at least 8, was able to elicit a strong oxidative burst reaction in heterologous N. tabacum suspension cell cultures (Figure 10). Now this OGA fraction was tested in homologous C. annuum suspension cell cultures. Samples were added to the C. annuum culture to a final concentration of 5 mg/ml (). A negative control contained only water (♦). Once more this OGA fraction evoked a strong oxidative burst, similar to the reaction in N. tabacum. These observations show that OGAs with a DP of at least 8 that were generated by an X. campestris pv. campestris culture from co-incubated C. annuum cell wall material are a powerful endogenous elicitor.

To further verify the role of the TonB system core genes and particular exbD2 in generating the OGA DAMP, we resumed analyzing the mutants deficient in these genes [64, 66]. Cell-free supernatants of X. campestris pv. campestris cultivations that had been co-incubated with C. annuum cell wall material had been shown to induce oxidative burst reactions in suspension cell cultures of non-host plants (Figure 4), while the supernatant of an analogously cultivated mutant strain deficient in exbD2 evoked no oxidative burst in a non-host suspension cell culture (Figure 5). Now we tested the effect of cell-free supernatants obtained from co-incubating X. campestris pv. campestris strains with pectin on non-host cell suspension cultures concerning their ability to induce oxidative burst reactions. Mutants deficient in all genes of the X. campestris pv. campestris TonB core system including exbD2 were tested in this approach, and turned out to be clearly affected in evoking oxidative burst reactions. The oxidative burst reactions in non-host suspension cell cultures were recovered when the disrupted genes were complemented specifically with complete copies of the respective genes (Additional file 4). The hydrogen peroxide concentrations measured in response to aliquots of cell-free supernatants from cultivations of the complemented mutants in the presence of pectin was at least at wild-type level. This clearly underlines that the genes of the bacterial TonB core system including exbD2 are involved in linking the bacterial response to the presence of pectin with a specific defense reaction of non-host plants.


Most bacterial pathogens produce a wide variety of cell wall degrading enzymes like endoglucanases, cellulases, pectinases, hemicellulases and lyases. In case of X. campestris pv. campestris, genes coding for almost 30 enzymes probably involved in cell-wall degradation were identified based on genome data [43, 45, 46]. Among them were several genes involved in degrading polygalacturonic acid (Additional file 5: Table S2). In consequence, cell wall degradation by X. campestris pv. campestris is assumed to result in the release of a complex mixture of poly- and oligosaccharides to the surrounding medium. It is in the best advantage of plants to recognize such signals of microbial pathogenicity as DAMPs in order to initiate suitable defense reactions. Plants are able to perceive diverse signal molecules such as the yeast elicitor in tobacco [70], bacterial flagellin [71, 72], harpin proteins [59], Hrp proteins from X. campestris[31], fungal proteins in parsley [73] and fungal exoenzymes in tobacco [74]. Rouet-Mayer et al. were also able to show that fungal lyase represents a different chemical stimulus than the OGAs produced from the cell walls by this enzyme’s activity and that both these elicitors despite their common origin activated at least partially differing signal transduction pathways. The fact that tobacco is not only able to perceive the products of enzymatic digestion, but also the enzyme itself, shows how crucial it is for the plant to recognize the pathogenic fungus.

Here we report on the release of elicitor-active compounds obtained from the co-incubation of C. annuum cell walls with X. campestris pv. campestris. The co-incubation was carried out using a crude cell wall extract from pepper leafs and the X. campestris pv. campestris strain Bac2. The use of crude cell wall extracts instead of complete plants or leafs has the advantage that all products resulting from the incubation can originate only from the plant cell wall material or the bacteria. Orientation experiments indicated that cell wall-derived oligosaccharides were responsible for the elicitor activity. To identify the elicitor-active compound, HPAE chromatography [75] was employed. First hints on the origin of the elicitor-active molecules were obtained by analyzing the composition of neutral sugars and uronic acids. In comparison to the controls, an increased abundance of typical cell wall sugars was observed when X. campestris pv. campestris and cell-free pepper cell wall material were co-incubated. In the subsequent characterization of the oligosaccharide composition using HPAEC [76], UV absorption was measured in addition to the PAD signal in order to detect double-bonds in the newly formed oligosaccharides. This resulted in identifying the elicitor-active compounds as pectin fragments with a varying degree of polymerization (DP) by comparing the elution profile to a standard derived from pectin digested by a pectate lyase from a commercially supplier. MALDI-TOF MS was used as a valuable tool to obtain further structural information on the isolated oligosaccharides. These fragments with different DPs were then isolated with preparative HPAEC and tested for their elicitor activities.

The highest elicitor activity was found for OGAs with a DP exceeding 8. This result corresponds well with data from Svalheim & Robertson [77], who showed that OGAs released by fungal enzymes with DPs ranging from 9 to12 are able to elicit oxidative burst reactions in cucumber hypocotyl segments. It also fits well with other data summarized by Ryan [78], showing that different oligosaccharides induce a vast variety of plant defense responses. For example, oligomeric fragments of chitosan with DPs ranging from 6 to 11 are able to induce defensive mechanisms in tissues of several plants. OGAs with a DP below 9 are unable to induce phytoalexin production in soybean cotyledons [20], which corresponds well with the X. campestris pv. campestris – pepper system, where most of the elicitor activity resides in OGAs of a DP exceeding 8.

Interestingly, OGAs can have different roles in other plant-pathogen interactions. In wheat plants, small oligomers of galacturonic acid (dimers and trimers) have a completely different function as they act as suppressors of the plant pathogen defense and thereby promote the growth of pathogenic fungi [76]. In A. thaliana, where WAK1 was recently identified as OGA receptor [21, 23], only small cell wall-derived OGAs with DPs of 2 to 6 have been reported to induce genes involved in the plant response to cell wall-degrading enzymes from the pathogen E. carotovora[79].

Plants need to permanently monitor whether there are indications for pathogen attack, a task that is not trivial as it requires to efficiently filter pathogen-related signals from others, like those generated by commensal or symbiotic microorganism. For each plant it is of fundamental importance to decide correctly whether to initiate defense or not, as defense includes expensive measures like sacrificing plant tissue by intentional cell death at the assumed infection site, while mistakenly omitted defense can be lethal [80]. Analyzing the interaction of pathogens with non-host plants is an approach to identify the molecular nature of plant-pathogen interactions. Beside the highly specific recognition of avr gene products interactions with host plants [81], lipopolysaccharides [26, 27], muropeptides [30], hrp gene products [31], secreted proteins [82] and the pectate-derived DAMP described in this study contribute to the reaction of non-host cells in response to Xanthomonas. Obviously, all these MAMPs and DAMPs are part of the very complex and specific damage- and microbe-associated molecular signal, where individual elicitors contribute in a complex manner [83] to obtain an optimal decision of the plant whether to initiate defense with all its costly consequences or not.

While the A. thaliana OGA receptor WAK1 was recently identified [21, 23], it is now fascinating to see that the generation of a DAMP similar to that perceived by WAK1 is related to bacterial trans-envelope signaling. Recently, trans-envelope signaling has been shown to be a second important function of TonB systems [61, 84] besides the uptake of nutrients. A model describing this signaling mechanism assumes that members of a specific subgroup of the TonB-dependent receptors, which share a common N-terminal extension and which were termed TonB-dependent transducers, perceive an environmental signal in the outer membrane [84]. Such TonB-dependent transducers are energized via the TonB-ExbB-ExbD core complex, while their N-terminal extension permits contacting periplasmic structures of anti-sigma factors that are localized in the inner membrane. The anti-sigma factors can then interact with ECF family sigma factors [84, 85], which can modulate bacterial gene expression at the transcriptional level. Probably the best understood paradigm for TonB-dependent trans-envelope signaling is the Fec signaling pathway of E. coli[61]. The exbD2 gene product of X. campestris pv. campestris B100 seems involved in trans-envelope signaling via the TonB system, while the exbD1 gene is also required to import substances like ferric iron [64]. However the situation could be more complex, as exbD2 might also be involved in uptake of cell wall degradation products, and as exbD1 might be involved in further so far unidentified signaling processes. Currently there is no evidence that the products of both genes are involved in both functions, transportation and signaling. But likewise, so far there is no reason to assume strict task sharing, where the exbD1 gene product is exclusively required for transport, while ExbD2 is specialized on signaling.

Further research could shed more light on the processes involved in bacterial reaction to the presence of pectin. Obviously, extracellular pectin-degrading enzymes are induced. But it is completely unclear which mechanisms are involved, and what kind of role the TonB core system plays. It could be just involved in importing polygalacturonic acid or derivatives of it. Imported galacturonic acid compounds could be perceived by an intracellular factor like a transcriptional regulator. Alternatively, the TonB system could be directly involved in signaling via an anti-sigma factor as described by Koebnik [84]. Further more, there is no reason to exclude regulatory processes at post-transcriptional levels. Likewise, the specific roles of the enzymes involved in pectin degradation are unclear. The genome of X. campestris pv. campestris B100 includes six genes of enzymes that cleave the glycosidic bonds between adjacent glucuronic acid residues (Additional file 5: Table S2). The product of the polygalacturonase gene pglA2 is similar to a recently characterized X. fastidiosa enzyme [48], and the truncated pectate lyase encoded by pel4 is partially similar to an enzyme from Pseudomonas cellulosa[86], but seemed to lack the carbohydrate-binding module (CBM) [87] of the P. cellulosa enzyme. A polygalacturonate-induced gene for an X. campestris pv. campestris TonB-dependent receptor like two neighboring genes encoding degrading enzymes [62] are promising candidates for being involved in the response to the presence of polygalacturonate. However, like for other Xanthomonas enzymes that degrade plant cell-wall constituents, the kinetic properties of the pectin-degrading enzymes are not known, nor is there evidence for the regulation and expression of their genes or for regulatory processes that directly address the enzymes.


As far as we know, we report here for the first time on a DAMP that is produced by Xanthomonas exoenzymes from non-host plant cell walls. With the characterization of a DAMP produced by X. campestris pv. campestris, which was identified as an OGA, we were able to identify a further component of the complex network of signals that determines whether a plant is a host for X. campestris pv. campestris or whether it is resistant to this pathogen. So far, DAMPs were mainly known to be generated by fungal pathogens [1720], and so far there are rather few examples where the signaling mechanisms have been analyzed profoundly at a molecular level. Due to the reduced complexity of prokaryotes, spending more effort on analyzing bacteria-generated DAMPs may also be a promising complement to studying fungi-based systems for pragmatic reasons, as experiments may be simpler in design, with the additional perspective of utilizing results provided by high-throughput approaches in the genomics and post-genomics disciplines for many bacteria.

This work gives plausible evidence that ExbD2 is involved in transducing information on the presence of plant cell wall-derived material in the bacterial environment to the interior of the bacterial cell, leading to bacterial pectate lyase activity in the extracellular medium, which in return provokes the defense of non-host plants that can be monitored by measuring the oxidative burst reaction (Figure 12). Thus, the exbD2 gene product seems involved in trans-envelope signaling via the TonB system.

Figure 12
figure 12

Schematic overview on the interactions of X. campestris pv. campestris and C. annuum analyzed in this work. A major plant cell wall component is pectate, a polygalacturonide (PGA). Pectate is perceived by X. campestris pv. campestris by means of the TonB system. ExbD2, which is not required for ferric iron uptake, is essential for this process. This induces extracellular pectate lyase activity, resulting in the generation of OGAs. Extracellular OGAs consisting of at least 8 galacturonate residues are recognized by C. annuum as a DAMP, resulting in the initiation of defensive measures like an oxidative burst reaction. The presence of a PRR similar to WAK1 is supposed for C. annuum. WAK1 has been identified recently in A. thaliana as a receptor that specifically perceives OGAs [23].

Against the emerging background of TonB-related signal transduction [84] it is not too surprising to see an isoform of ExbD being involved in signaling. Nevertheless, the experimental results that indicate the involvement of ExbD2 in transducing a plant cell-wall-derived signal raise the question whether the E. coli paradigm on tonB functionality needs to be adapted or extended for X. campestris pv. campestris, as in E. coli ExbD (like ExbB) is supposed to be involved in signaling exclusively by contributing to energizing the outer membrane TonB-dependent transducer via TonB. The specific involvement of ExbD2 in signaling may indicate a more direct role of this ExbD isoform in signal transduction.


Cultivation of Xanthomonas campestrispv. campestris

The bacterial strains and plasmids used in this study are listed in Table 1. Unless otherwise stated, X. campestris pv. campestris was grown at 30°C on solid TY medium (5 g tryptone, 3 g yeast extract, 0.4 g CaCl2, The bacterial strains and plasmids used in this study are listed in Table 1, 12 g agar, per l), for strain B100-Bac2 supplemented with 150 mg bacitracin per l. For the X. campestris pv. campestris strains B100-5.05, B100-7.03, and B100-9.01, the medium was supplemented with FeSO4 to a final concentration of 100 mM as described previously [64]. Alternatively, bacteria were grown in modified liquid M9 minimal medium supplemented with 0.05% casamino acids [88]. Unless otherwise specified, minimal medium was supplemented with glucose or polygalacturonic acid at final concentrations of 2% or 0.25%, respectively. Streptomycin, kanamycin, gentamicin, and chloramphenicol were added to the media when appropriate in concentrations of 800 mg per l, 80 mg per l, 20 mg per l, and 100 mg per l, respectively.

Table 1 Bacterial strains and plasmids used in this study

Cultivation of C. annuumplants

C. annuum (cultivar California Wonder) plants derived from seedlings were grown in the greenhouse at 21°C with 12/12 day/night hours. Cell wall material was isolated from 6 weeks old plants.

Analysis of enzyme activity

Extracellular pectate lyase activity was monitored by an agar plate test and quantified in a photometric assay [38]. For the pectate lyase assay, X. campestris pv. campestris cultures were grown for 24 h in M9 medium supplemented with pectate and FeSO4. The resulting values were calibrated to the activity of glucose-6-phosphate dehydrogenase. For the tests on agar plates [92], X. campestris pv. campestris strains were cultivated for 2 days on M9 medium supplemented with pectate and FeSO4 as described elsewhere [93].

Genome analysis and recombinant DNA procedures

Genome data were analyzed and visualized by means of the GenDB annotation system [94]. The EDGAR software [95] was employed to compare complete Xanthomonas genomes that were available from public databases [42, 43, 45, 46, 9699]. For the analysis of genes encoding polysaccharide-degrading enzymes, information provided by the CAZy database ( has been considered [100].

All cloning was performed applying standard methods [101] and as described previously [64, 66]. An 11.1 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 carrying the pglI gene in cosmid pIJ3051 [39] was inserted into the plasmid vector pHGW31 to obtain plasmid pHGW260. A 3.8 kb BamHI-ClaI sub-fragment with the pglI gene was then transferred to the cloning vectors pBCKS+ and pBCSK+, resulting in the plasmids pHGW261 and pHGW262, respectively. In pHGW262, pglI was constitutively expressed in E. coli from the lac promoter of the pBCSK+ multiple cloning site. To express pglI also in X. campestris pv. campestris, pHGW267 was constructed by cloning the 3.8 kb BamHI-ClaI sub-fragment with the X. campestris pv. campestris 8004 pglI gene into the multiple cloning site of pUC6S (Apr) [90], where it was under the control of the constitutive Pout promoter of the aacC1 gene from pMS246 [91], which was cloned as a 1 kb BamHI fragment into the BamHI site upstream of pglI.

Isolation of plant cell wall material

Leafs of C. annuum were employed to obtain cell wall material. Leafs (30 g) were homogenized in 150 ml sodium acetate (50mM, pH 5) for 3 min and filtered with a fluted filter. After the filtration, the cell wall material was washed with 1 l sodium acetate (4°C), 1 l ethanol (4°C) and with 1 l acetone (−20°C). The washed material was then air dried at room temperature and stored under inert atmosphere at -20°C.

Co-incubation of X. campestris pv. campestris and C. annuumcell wall material

5 ml X. campestris pv. campestris over-night liquid culture was centrifuged. After removal of the supernatant, the sediment was re-suspended in 5 ml phosphate buffer (50 mM potassium phosphate, pH 7.9). 100 mg of the isolated cell wall material was then added to this solution and incubated over night at 28°C. The sample was then centrifuged and the pellet discarded. After heating (5 min; 100°C), centrifugation (10 min 10,000×g) and dialysis (molecular weight cut off 1000), the sample was freeze-dried. Resuspended lyophilized material was then used for further experiments.

Removing LPS from the samples via polymyxin B agarose

X. campestris pv. campestris lipopolysaccharides (LPSs) were removed from the elicitor preparation using a batch technique by adding an excess amount of polymyxin B agarose [102] as described in [103]. Upon addition of polymyxin B agarose (Sigma-Aldrich), the samples were shaken and centrifuged. While the pellet probably containing LPS bound to polymyxin B agarose was discarded, the supernatant was used for further analyses.

Identification, isolation and characterization of oligosaccharides

The analyses of oligosaccharides was performed by HPAEC using a DIONEX GP-40 gradient pump; a Merck-Hitachi D-2000 Chromato Integrator; a DIONEX pulsed amperometric detector and a DIONEX UV detector.

Monosaccharide composition of isolated oligosaccharides was analyzed upon acid hydrolysis in trifluoroacetic acid (2 M; 120°C for 2 h). Neutral sugars were separated and identified using an isocratic elution (10 mM sodium hydroxide; flow 1 ml/min) with amperometric detection on a CarboPac® PA-100 column. For charged sugars a linear sodium acetate gradient ranging from 0.02 M to 0.5 M under alkaline conditions (0.1 M NaOH) with a flow rate of 1 ml/min was used [75].

Pectate fragments were separated using a sodium acetate gradient (ranging from 0.01 M to 1.0 M with a plateau of 10 min. at a concentration 0.7 M sodium acetate; 0.1 M NaOH; CarboPac® PA-100 column; flow 1 ml/min). For the identification of pectate fragments a pectate standard was generated by digestion of pectin (Pectin esterified, Sigma P-9561) by pectate lyase (Sigma P-7052). The isolation of pectate fragments was carried out under the conditions described above, but a semi-preparative column (CarboPac® PA-1; flow 2.5 ml/min) was used.

MALDI-TOF MS of isolated oligosaccharides

Crude extracts were analyzed on a Bruker ultraflex I MALDI-TOF mass spectrometer (Bruker-Daltonics, Bremen, Germany) in the negative–ion mode. Samples were analyzed in the linear and in the reflector TOF. Gentisic acid was used as matrix. For sample preparation, 1 μl saturated gentisic acid solution was mixed with 1 μl of 50 mg ml–1 crude extract lyophilisate dissolved in demineralized water. One microliter of this mixture was dropped onto the MALDI target.

Determination of the oxidative burst reaction in plant cell suspension cultures

The detection of the oxidative burst was performed using the H2O2-dependent chemiluminescence reaction described by Warm [104]. Three to five days after sub-cultivation 2 g of cell material from the cell suspension cultures was diluted in 8 ml of pre-incubation medium (3% w/v sucrose in 0.04 × MS [105]) and incubated for 3 to 4 hours. For the measurement of the oxidative burst 200 μl aliquots of these suspensions were mixed with phosphate buffer (50 mM potassium phosphate, pH 7.9) and 1.2 mM luminol in the same phosphate buffer. The reaction was started by the addition of 100 μl of 14 mM potassium hexacyanate. The luminescence was measured with a Luminometer 1250 (BioOrbit, Turku, Finland). The intensity of luminescence was calibrated for hydrogen peroxide concentrations of 0.01 mM, to 0.05 mM.


Polygalacturonic acid (sodium salt), pectin and polymyxin B agarose was from Sigma-Aldrich, Taufkirchen, Germany. Unless otherwise specified, other chemicals were obtained from Merck, Darmstadt, Germany.





Base pair


Carbohydrate-binding Module


Protein-coding Sequence




Damage-associated Molecular Pattern


Diffusible Factor


Degree of Polymerization


Diffusible Signal Factor








High-performance Anion Exchange


High-performance Anion Exchange Chromatography


High-performance Liquid Chromatography


Hypersensitive Response








Matrix-assisted Laser Desorption/ionization time-of-flight


Microbe-associated Molecular pattern




Mass Spectroscopy




Pathogen-associated Molecular Pattern




Pattern Recognition Receptor


PAMP-triggered Immunity






Reactive Oxygen Species






  1. Jones JD, Dangl JL: The plant immune system. Nature. 2006, 444 (7117): 323-329. 10.1038/nature05286.

    PubMed  CAS  Google Scholar 

  2. Boller T, Felix G: A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol. 2009, 60: 379-406. 10.1146/annurev.arplant.57.032905.105346.

    PubMed  CAS  Google Scholar 

  3. Bauer Z, Gomez-Gomez L, Boller T, Felix G: Sensitivity of different ecotypes and mutants of Arabidopsis thaliana toward the bacterial elicitor flagellin correlates with the presence of receptor-binding sites. J Biol Chem. 2001, 276 (49): 45669-45676. 10.1074/jbc.M102390200.

    PubMed  CAS  Google Scholar 

  4. Lamb C, Dixon RA: The oxidative burst in plant disease resistance. Annu Rev Plant Physiol Plant Mol Biol. 1997, 48: 251-275. 10.1146/annurev.arplant.48.1.251.

    PubMed  CAS  Google Scholar 

  5. Wei ZM, Laby RJ, Zumoff CH, Bauer DW, He SY, Collmer A, Beer SV: Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science. 1992, 257 (5066): 85-88. 10.1126/science.1621099.

    PubMed  CAS  Google Scholar 

  6. Yap MN, Rojas CM, Yang CH, Charkowski AO: Harpin mediates cell aggregation in Erwinia chrysanthemi 3937. J Bacteriol. 2006, 188 (6): 2280-2284. 10.1128/JB.188.6.2280-2284.2006.

    PubMed  CAS  PubMed Central  Google Scholar 

  7. Kim JG, Jeon E, Oh J, Moon JS, Hwang I: Mutational analysis of Xanthomonas harpin HpaG identifies a key functional region that elicits the hypersensitive response in nonhost plants. J Bacteriol. 2004, 186 (18): 6239-6247. 10.1128/JB.186.18.6239-6247.2004.

    PubMed  CAS  PubMed Central  Google Scholar 

  8. Li P, Lu X, Shao M, Long J, Wang J: Genetic diversity of harpins from Xanthomonas oryzae and their activity to induce hypersensitive response and disease resistance in tobacco. Sci China C Life Sci. 2004, 47 (5): 461-469. 10.1360/03yc0152.

    PubMed  CAS  Google Scholar 

  9. Alfano JR, Bauer DW, Milos TM, Collmer A: Analysis of the role of the Pseudomonas syringae pv. syringae HrpZ harpin in elicitation of the hypersensitive response in tobacco using functionally non-polar hrpZ deletion mutations, truncated HrpZ fragments, and hrmA mutations. Mol Microbiol. 1996, 19 (4): 715-728. 10.1046/j.1365-2958.1996.415946.x.

    PubMed  CAS  Google Scholar 

  10. Midland SL, Keen NT, Sims JJ, Midland MM, Stayton MM, Burton V, Smith MJ, Mazzola EP, Graham KJ, Clardy J: The structures of syringolide-1 and syringolide-2, novel C-glycosidic elicitors from Pseudomonas syringae pv tomato. J Org Chem. 1993, 58 (11): 2940-2945. 10.1021/jo00063a007.

    CAS  Google Scholar 

  11. Silipo A, Erbs G, Shinya T, Dow JM, Parrilli M, Lanzetta R, Shibuya N, Newman MA, Molinaro A: Glyco-conjugates as elicitors or suppressors of plant innate immunity. Glycobiology. 2010, 20 (4): 406-419. 10.1093/glycob/cwp201.

    PubMed  CAS  Google Scholar 

  12. Lotze MT, Zeh HJ, Rubartelli A, Sparvero LJ, Amoscato AA, Washburn NR, Devera ME, Liang X, Tor M, Billiar T: The grateful dead: damage-associated molecular pattern molecules and reduction/oxidation regulate immunity. Immunol Rev. 2007, 220: 60-81. 10.1111/j.1600-065X.2007.00579.x.

    PubMed  CAS  Google Scholar 

  13. Yamaguchi Y, Huffaker A: Endogenous peptide elicitors in higher plants. Curr Opin Plant Biol. 2011, 14 (4): 351-357. 10.1016/j.pbi.2011.05.001.

    PubMed  CAS  Google Scholar 

  14. Chai HB, Doke N: Superoxide anion generation: a response of potato leaves to infection with Phytophtera infestans. Phytopathology. 1987, 77: 645-649. 10.1094/Phyto-77-645.

    CAS  Google Scholar 

  15. Bergey DR, Orozco-Cardenas M, de Mouro DS, Ryan CA: A wound- and systemin-inducible polygalacturonase in tomato leaves. Proc Natl Acad Sci USA. 1999, 96: 1756-1760. 10.1073/pnas.96.4.1756.

    PubMed  CAS  PubMed Central  Google Scholar 

  16. Sharp JK, McNeil M, Albersheim P: The primary structures of one elicitor-active and seven elicitor-inactive hexa(beta-D-glucopyranosyl)-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f. sp. glycinea. J Biol Chem. 1984, 259 (18): 11321-11336.

    PubMed  CAS  Google Scholar 

  17. Hahn MG, Darvill AG, Albersheim P: Host-pathogen interactions: XIX. The endogenous elicitor, a fragment of the a plant cell wall polysaccharide that elicits phytoalexin accumulation in soybeans. Plant Physiol. 1981, 68 (5): 1161-1169. 10.1104/pp.68.5.1161.

    PubMed  CAS  PubMed Central  Google Scholar 

  18. Monden T, Nakamura H, Murai A: The sugar composition and partial structure of the self-induced endogenous elicitor from potato. Biochem Biophys Res Commun. 1995, 215 (2): 768-773. 10.1006/bbrc.1995.2529.

    PubMed  CAS  Google Scholar 

  19. Davis KR, Lyon GD, Darvill AG, Albersheim P: Host-pathogen interactions: XXV. Endopolygalacturonic acid lyase from Erwinia carotovora elicits phytoalexin accumulation by releasing plant cell wall fragments. Plant Physiol. 1984, 74 (1): 52-60. 10.1104/pp.74.1.52.

    PubMed  CAS  PubMed Central  Google Scholar 

  20. Nothnagel EA, McNeil M, Albersheim P, Dell A: Host-pathogen interactions: XXII. A galacturonic acid oligosaccharide from plant cell walls elicits phytoalexins. Plant Physiol. 1983, 71 (4): 916-926. 10.1104/pp.71.4.916.

    PubMed  CAS  PubMed Central  Google Scholar 

  21. Cabrera JC, Boland A, Messiaen J, Cambier P, Van Cutsem P: Egg box conformation of oligogalacturonides: the time-dependent stabilization of the elicitor-active conformation increases its biological activity. Glycobiology. 2008, 18 (6): 473-482. 10.1093/glycob/cwn027.

    PubMed  CAS  Google Scholar 

  22. Kohorn BD, Johansen S, Shishido A, Todorova T, Martinez R, Defeo E, Obregon P: Pectin activation of MAP kinase and gene expression is WAK2 dependent. Plant J. 2009, 60 (6): 974-982. 10.1111/j.1365-313X.2009.04016.x.

    PubMed  CAS  PubMed Central  Google Scholar 

  23. Brutus A, Sicilia F, Macone A, Cervone F, De Lorenzo G: A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides. Proc Natl Acad Sci USA. 2010, 107 (20): 9452-9457. 10.1073/pnas.1000675107.

    PubMed  CAS  PubMed Central  Google Scholar 

  24. Xanthomonas. 1993, Chapman & Hall, London

  25. Ryan RP, Vorhölter FJ, Potnis N, Jones JB, Van Sluys MA, Bogdanove AJ, Dow JM: Pathogenomics of Xanthomonas: understanding bacterium-plant interactions. Nat Rev Microbiol. 2011, 9 (5): 344-355. 10.1038/nrmicro2558.

    PubMed  CAS  Google Scholar 

  26. Meyer A, Pühler A, Niehaus K: The lipopolysaccharides of the phytopathogen Xanthomonas campestris pv. campestris induce an oxidative burst reaction in cell cultures of Nicotiana tabacum. Planta. 2001, 213 (2): 214-222. 10.1007/s004250000493.

    PubMed  CAS  Google Scholar 

  27. Newman MA, Daniels MJ, Dow JM: Lipopolysaccharide from Xanthomonas campestris induces defense-related gene expression in Brassica campestris. Mol Plant Microbe Interact. 1995, 8 (5): 778-780. 10.1094/MPMI-8-0778.

    PubMed  CAS  Google Scholar 

  28. Kaczynski Z, Braun S, Lindner B, Niehaus K, Holst O: Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide. J Endotoxin Res. 2007, 13 (2): 101-108. 10.1177/0968051907079121.

    PubMed  CAS  Google Scholar 

  29. Silipo A, Molinaro A, Sturiale L, Dow JM, Erbs G, Lanzetta R, Newman MA, Parrilli M: The elicitation of plant innate immunity by lipooligosaccharide of Xanthomonas campestris. J Biol Chem. 2005, 280 (39): 33660-33668. 10.1074/jbc.M506254200.

    PubMed  CAS  Google Scholar 

  30. Erbs G, Silipo A, Aslam S, De Castro C, Liparoti V, Flagiello A, Pucci P, Lanzetta R, Parrilli M, Molinaro A, et al: Peptidoglycan and muropeptides from pathogens Agrobacterium and Xanthomonas elicit plant innate immunity: structure and activity. Chem Biol. 2008, 15 (5): 438-448. 10.1016/j.chembiol.2008.03.017.

    PubMed  CAS  Google Scholar 

  31. Newman MA, von Roepenack-Lahaye E, Parr A, Daniels MJ, Dow JM: Induction of hydroxycinnamoyl-tyramine conjugates in pepper by Xanthomonas campestris, a plant defense response activated by hrp gene-dependent and hrp gene-independent mechanisms. Mol Plant Microbe Interact. 2001, 14 (6): 785-792. 10.1094/MPMI.2001.14.6.785.

    PubMed  CAS  Google Scholar 

  32. Büttner D, Bonas U: Regulation and secretion of Xanthomonas virulence factors. FEMS Microbiol Rev. 2010, 34 (2): 107-133. 10.1111/j.1574-6976.2009.00192.x.

    PubMed  Google Scholar 

  33. Bogdanove AJ, Schornack S, Lahaye T: TAL effectors: finding plant genes for disease and defense. Curr Opin Plant Biol. 2010, 13 (4): 394-401. 10.1016/j.pbi.2010.04.010.

    PubMed  CAS  Google Scholar 

  34. Cunnac S, Wilson A, Nuwer J, Kirik A, Baranage G, Mudgett MB: A conserved carboxylesterase is a suppressor of AvrBst-elicited resistence in Arabidopsis. Plant Cell. 2007, 19 (2): 688-705. 10.1105/tpc.106.048710.

    PubMed  CAS  PubMed Central  Google Scholar 

  35. Canonne J, Marino D, Jauneau A, Pouzet C, Briere C, Roby D, Rivas S: The Xanthomonas type III effector XopD targets the Arabidopsis transcription factor MYB30 to suppress plant defense. Plant Cell. 2011, 23 (9): 3498-3511. 10.1105/tpc.111.088815.

    PubMed  CAS  PubMed Central  Google Scholar 

  36. Szczesny R, Jordan M, Schramm C, Schulz S, Cogez V, Bonas U, Büttner D: Functional characterization of the Xcs and Xps type II secretion systems from the plant pathogenic bacterium Xanthomonas campestris pv vesicatoria. New Phytol. 2010, 187 (4): 983-1002. 10.1111/j.1469-8137.2010.03312.x.

    PubMed  CAS  Google Scholar 

  37. Nasuno S, Starr MP: Polygalacturonic acid trans-eliminase of Xanthomonas campestris. Biochem J. 1967, 104 (1): 178-185.

    PubMed  CAS  PubMed Central  Google Scholar 

  38. Dow JM, Scofield G, Trafford K, Turner PC, Daniels MJ: A gene cluster in Xanthomonas campestris pv. campestris required for pathogenicity controls the excretion of polygalacturonate lyase and other enzymes. Physiol Mol Plant Pathol. 1987, 31: 261-271. 10.1016/0885-5765(87)90070-1.

    CAS  Google Scholar 

  39. Dow JM, Milligan DE, Jamieson L, Barber CE, Daniels MJ: Molecular cloning of a polygalacturonate lyase gene from Xanthomonas campestris pv. campestris and role of the gene product in pathogenicity. Physiol Mol Plant P. 1989, 35: 113-120. 10.1016/0885-5765(89)90081-7.

    CAS  Google Scholar 

  40. Xiao Z, Boyd J, Grosse S, Beauchemin M, Coupe E, Lau PC: Mining Xanthomonas and Streptomyces genomes for new pectinase-encoding sequences and their heterologous expression in Escherichia coli. Appl Microbiol Biotechnol. 2008, 78 (6): 973-981. 10.1007/s00253-008-1389-2.

    PubMed  CAS  Google Scholar 

  41. Hsiao YM, Zheng MH, Hu RM, Yang TC, Tseng YH: Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF. Microbiology. 2008, 154 (Pt 3): 705-713.

    PubMed  CAS  Google Scholar 

  42. Bogdanove AJ, Koebnik R, Lu H, Furutani A, Angiuoli SV, Patil PB, Van Sluys MA, Ryan RP, Meyer DF, Han SW, et al: Two new complete genome sequences offer insight into host and tissue specificity of plant pathogenic Xanthomonas spp. J Bacteriol. 2011, 193 (19): 5450-5464. 10.1128/JB.05262-11.

    PubMed  CAS  PubMed Central  Google Scholar 

  43. da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, et al: Comparison of the genomes of two Xanthomonas pathogens with differing host specificities. Nature. 2002, 417 (6887): 459-463. 10.1038/417459a.

    PubMed  Google Scholar 

  44. He YQ, Zhang L, Jiang BL, Zhang ZC, Xu RQ, Tang DJ, Qin J, Jiang W, Zhang X, Liao J, et al: Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris. Genome Biol. 2007, 8 (10): R218-10.1186/gb-2007-8-10-r218.

    PubMed  PubMed Central  Google Scholar 

  45. Qian W, Jia Y, Ren SX, He YQ, Feng JX, Lu LF, Sun Q, Ying G, Tang DJ, Tang H, et al: Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris. Genome Res. 2005, 15 (6): 757-767. 10.1101/gr.3378705.

    PubMed  CAS  PubMed Central  Google Scholar 

  46. Vorhölter FJ, Schneiker S, Goesmann A, Krause L, Bekel T, Kaiser O, Linke B, Patschkowski T, Rückert C, Schmid J, et al: The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis. J Biotechnol. 2008, 134 (1–2): 33-45.

    PubMed  Google Scholar 

  47. Vorhölter FJ, Thias T, Meyer F, Bekel T, Kaiser O, Pühler A, Niehaus K: Comparison of two Xanthomonas campestris pathovar campestris genomes revealed differences in their gene composition. J Biotechnol. 2003, 106 (2–3): 193-202.

    PubMed  Google Scholar 

  48. Roper MC, Greve LC, Warren JG, Labavitch JM, Kirkpatrick BC: Xylella fastidiosa requires polygalacturonase for colonization and pathogenicity in Vitis vinifera grapevines. Mol Plant Microbe Interact. 2007, 20 (4): 411-419. 10.1094/MPMI-20-4-0411.

    PubMed  CAS  Google Scholar 

  49. He YW, Ng AY, Xu M, Lin K, Wang LH, Dong YH, Zhang LH: Xanthomonas campestris cell-cell communication involves a putative nucleotide receptor protein Clp and a hierarchical signalling network. Mol Microbiol. 2007, 64 (2): 281-292. 10.1111/j.1365-2958.2007.05670.x.

    PubMed  CAS  Google Scholar 

  50. Tao J, He C: Response regulator, VemR, positively regulates the virulence and adaptation of Xanthomonas campestris pv. campestris. FEMS Microbiol Lett. 2010, 304 (1): 20-28. 10.1111/j.1574-6968.2009.01892.x.

    PubMed  CAS  Google Scholar 

  51. Huang DL, Tang DJ, Liao Q, Li XQ, He YQ, Feng JX, Jiang BL, Lu GT, Tang JL: The Zur of Xanthomonas campestris is involved in hypersensitive response and positively regulates the expression of the hrp cluster via hrpX but not hrpG. Mol Plant Microbe Interact. 2009, 22 (3): 321-329. 10.1094/MPMI-22-3-0321.

    PubMed  Google Scholar 

  52. Jittawuttipoka T, Sallabhan R, Vattanaviboon P, Fuangthong M, Mongkolsuk S: Mutations of ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence of Xanthomonas campestris pv. campestris. Arch Microbiol. 2010, 192 (5): 331-339. 10.1007/s00203-010-0558-8.

    PubMed  CAS  Google Scholar 

  53. Ryan RP, Dow JM: Communication with a growing family: diffusible signal factor (DSF) signaling in bacteria. Trends Microbiol. 2011, 19 (3): 145-152. 10.1016/j.tim.2010.12.003.

    PubMed  CAS  Google Scholar 

  54. He YW, Wu J, Zhou L, Yang F, He YQ, Jiang BL, Bai L, Xu Y, Deng Z, Tang JL, et al: Xanthomonas campestris diffusible factor is 3-hydroxybenzoic acid and is associated with xanthomonadin biosynthesis, cell viability, antioxidant activity, and systemic invasion. Mol Plant Microbe Interact. 2011, 24 (8): 948-957. 10.1094/MPMI-02-11-0031.

    PubMed  CAS  Google Scholar 

  55. Qian W, Han ZJ, He C: Two-component signal transduction systems of Xanthomonas spp.: a lesson from genomics. Mol Plant Microbe Interact. 2008, 21 (2): 151-161. 10.1094/MPMI-21-2-0151.

    PubMed  CAS  Google Scholar 

  56. Qian W, Han ZJ, Tao J, He C: Genome-scale mutagenesis and phenotypic characterization of two-component signal transduction systems in Xanthomonas campestris pv. campestris ATCC 33913. Mol Plant Microbe Interact. 2008, 21 (8): 1128-1138. 10.1094/MPMI-21-8-1128.

    PubMed  CAS  Google Scholar 

  57. Zhang SS, He YQ, Xu LM, Chen BW, Jiang BL, Liao J, Cao JR, Liu D, Huang YQ, Liang XX, et al: A putative colRXC1049-colSXC1050 two-component signal transduction system in Xanthomonas campestris positively regulates hrpC and hrpE operons and is involved in virulence, the hypersensitive response and tolerance to various stresses. Res Microbiol. 2008, 159 (7–8): 569-578.

    PubMed  CAS  Google Scholar 

  58. He YW, Boon C, Zhou L, Zhang LH: Co-regulation of Xanthomonas campestris virulence by quorum sensing and a novel two-component regulatory system RavS/RavR. Mol Microbiol. 2009, 71 (6): 1464-1476. 10.1111/j.1365-2958.2009.06617.x.

    PubMed  CAS  Google Scholar 

  59. Postle K: TonB system, in vivo assays and characterization. Methods Enzymol. 2007, 422: 245-269.

    PubMed  CAS  Google Scholar 

  60. Noinaj N, Guillier M, Barnard TJ, Buchanan SK: TonB-dependent transporters: regulation, structure, and function. Annu Rev Microbiol. 2010, 64: 43-60. 10.1146/annurev.micro.112408.134247.

    PubMed  CAS  PubMed Central  Google Scholar 

  61. Braun V, Endriss F: Energy-coupled outer membrane transport proteins and regulatory proteins. Biometals. 2007, 20 (3–4): 219-231.

    PubMed  CAS  Google Scholar 

  62. Blanvillain S, Meyer D, Boulanger A, Lautier M, Guynet C, Denance N, Vasse J, Lauber E, Arlat M: Plant carbohydrate scavenging through TonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria. PLoS One. 2007, 2 (2): e224-10.1371/journal.pone.0000224.

    PubMed  PubMed Central  Google Scholar 

  63. Boulanger A, Dejean G, Lautier M, Glories M, Zischek C, Arlat M, Lauber E: Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris. J Bacteriol. 2010, 192 (6): 1487-1497. 10.1128/JB.01418-09.

    PubMed  CAS  PubMed Central  Google Scholar 

  64. Wiggerich HG, Klauke B, Köplin R, Priefer UB, Puhler A: Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not. J Bacteriol. 1997, 179 (22): 7103-7110.

    PubMed  CAS  PubMed Central  Google Scholar 

  65. Hung CH, Yang CF, Yang CY, Tseng YH: Involvement of tonB-exbBD1D2 operon in infection of Xanthomonas campestris phage phi L7. Biochem Biophys Res Commun. 2003, 302 (4): 878-884. 10.1016/S0006-291X(03)00255-9.

    PubMed  CAS  Google Scholar 

  66. Wiggerich HG, Pühler A: The exbD2 gene as well as the iron-uptake genes tonB, exbB and exbD1 of Xanthomonas campestris pv. campestris are essential for the induction of a hypersensitive response on pepper (Capsicum annuum). Microbiology. 2000, 146 (Pt 5): 1053-1060.

    PubMed  CAS  Google Scholar 

  67. Alvarez B, Alvarez J, Menendez A, Guijarro JA: A mutant in one of two exbD loci of a TonB system in Flavobacterium psychrophilum shows attenuated virulence and confers protection against cold water disease. Microbiology. 2008, 154 (Pt 4): 1144-1151.

    PubMed  CAS  Google Scholar 

  68. Shirley M, Lamont IL: Role of TonB1 in pyoverdine-mediated signaling in Pseudomonas aeruginosa. J Bacteriol. 2009, 191 (18): 5634-5640. 10.1128/JB.00742-09.

    PubMed  CAS  PubMed Central  Google Scholar 

  69. Gross A, Kapp D, Nielsen T, Niehaus K: Endocytosis of Xanthomonas campestris pathovar campestris lipopolysaccharides in non-host plant cells of Nicotiana tabacum. New Phytol. 2005, 165 (1): 215-226.

    PubMed  CAS  Google Scholar 

  70. Baier R, Schiene K, Kohring B, Flaschel E, Niehaus K: Alfalfa and tobacco cells react differently to chitin oligosaccharides and Sinorhizobium meliloti nodulation factors. Planta. 1999, 210 (1): 157-164. 10.1007/s004250050665.

    PubMed  CAS  Google Scholar 

  71. Felix G, Duran JD, Volko S, Boller T: Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J. 1999, 18 (3): 265-276. 10.1046/j.1365-313X.1999.00265.x.

    PubMed  CAS  Google Scholar 

  72. Gomez-Gomez L, Boller T: Flagellin perception: a paradigm for innate immunity. Trends Plant Sci. 2002, 7 (6): 251-256. 10.1016/S1360-1385(02)02261-6.

    PubMed  CAS  Google Scholar 

  73. Nürnberger T, Wirtz W, Nennstiel D, Hahlbrock K, Jabs T, Zimmermann S, Scheel D: Signal perception and intracellular signal transduction in plant pathogen defense. J Recept Signal Transduct Res. 1997, 17 (1–3): 127-136.

    PubMed  Google Scholar 

  74. Rouet-Mayer M-A, Mathieu Y, Cazale A-C, Guern J, Lauriere C: Extracellular alkalinization and oxidative burst induced by fungal lyase in tobacco cells are not due to the perception of oligogalacturonide fragments. Plant Physiol Biochem. 1997, 35 (4): 321-330.

    CAS  Google Scholar 

  75. Hardy MR, Townsend RR: Separation of positional isomers of oligosaccharides and glycopeptides by high-performance anion-exchange chromatography with pulsed amperometric detection. Proc Natl Acad Sci USA. 1988, 85 (10): 3289-3293. 10.1073/pnas.85.10.3289.

    PubMed  CAS  PubMed Central  Google Scholar 

  76. Moerschbacher BM, Mierau M, Graessner B, Noll U, Mort AJ: Small oligomers of galacturonic acid are endogenous suppressors of disease resistance reactions in wheat leaves. J Exp Bot. 1999, 50 (334): 605-612.

    CAS  Google Scholar 

  77. Svalheim O, Robertsen B: Elicitation of H2O2 production in cucumber hypocotyl segments by oligo-1,4-alpha-D-galacturonides and an oligo-beta-glucan preparation from cell walls of Phythophthora megasperma F Sp glycinea. Physiol Plantarum. 1993, 88 (4): 675-681. 10.1111/j.1399-3054.1993.tb01388.x.

    CAS  Google Scholar 

  78. Ryan CA: Oligosaccharides as recognition signals for the expression of defensive genes in plants. Biochemistry. 1988, 27 (25): 8879-8883. 10.1021/bi00425a001.

    CAS  Google Scholar 

  79. Norman C, Vidal S, Palva ET: Oligogalacturonide-mediated induction of a gene involved in jasmonic acid synthesis in response to the cell-wall-degrading enzymes of the plant pathogen Erwinia carotovora. Mol Plant Microbe Interact. 1999, 12 (7): 640-644. 10.1094/MPMI.1999.12.7.640.

    PubMed  CAS  Google Scholar 

  80. Stamp N: Out of the quagmire of plant defense hypotheses. Q Rev Biol. 2003, 78 (1): 23-55. 10.1086/367580.

    PubMed  Google Scholar 

  81. Büttner D, Bonas U: Common infection strategies of plant and animal pathogenic bacteria. Curr Opin Plant Biol. 2003, 6 (4): 312-319. 10.1016/S1369-5266(03)00064-5.

    PubMed  Google Scholar 

  82. Kunze G, Zipfel C, Robatzek S, Niehaus K, Boller T, Felix G: The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. Plant Cell. 2004, 16 (12): 3496-3507. 10.1105/tpc.104.026765.

    PubMed  CAS  PubMed Central  Google Scholar 

  83. Aslam SN, Erbs G, Morrissey KL, Newman MA, Chinchilla D, Boller T, Molinaro A, Jackson RW, Cooper RM: Microbe-associated molecular pattern (MAMP) signatures, synergy, size and charge: influences on perception or mobility and host defence responses. Mol Plant Pathol. 2009, 10 (3): 375-387. 10.1111/j.1364-3703.2009.00537.x.

    PubMed  CAS  Google Scholar 

  84. Koebnik R: TonB-dependent trans-envelope signalling: the exception or the rule?. Trends Microbiol. 2005, 13 (8): 343-347. 10.1016/j.tim.2005.06.005.

    PubMed  CAS  Google Scholar 

  85. Bordes P, Lavatine L, Phok K, Barriot R, Boulanger A, Castanie-Cornet MP, Dejean G, Lauber E, Becker A, Arlat M, et al: Insights into the extracytoplasmic stress response of Xanthomonas campestris pv. campestris: role and regulation of σE-dependent activity. J Bacteriol. 2011, 193 (1): 246-264. 10.1128/JB.00884-10.

    PubMed  CAS  PubMed Central  Google Scholar 

  86. Brown IE, Mallen MH, Charnock SJ, Davies GJ, Black GW: Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module. Biochem J. 2001, 355 (Pt 1): 155-165.

    PubMed  CAS  PubMed Central  Google Scholar 

  87. Guillén D, Sánchez S, Rodríguez-Sanoja R: Carbohydrate-binding domains: multiplicity of biological roles. Appl Microbiol Biotechnol. 2010, 85 (5): 1241-1249. 10.1007/s00253-009-2331-y.

    PubMed  Google Scholar 

  88. Vorhölter FJ, Niehaus K, Pühler A: Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris: a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen and the LPS core. Mol Genet Genomics. 2001, 266 (1): 79-95. 10.1007/s004380100521.

    PubMed  Google Scholar 

  89. Bullock WO, Fernandez JM, Short JM: XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques. 1987, 5 (4): 376-379.

    CAS  Google Scholar 

  90. Vieira J, Messing J: New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene. 1991, 100: 189-194.

    PubMed  CAS  Google Scholar 

  91. Becker A, Schmidt M, Jäger W, Pühler A: New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene. 1995, 162 (1): 37-39. 10.1016/0378-1119(95)00313-U.

    PubMed  CAS  Google Scholar 

  92. Hagerman AE, Blau DM, McClure AL: Plate assay for determining the time of production of protease, cellulase, and pectinases by germinating fungal spores. Anal Biochem. 1985, 151 (2): 334-342. 10.1016/0003-2697(85)90184-8.

    PubMed  CAS  Google Scholar 

  93. Hsiao YM, Fang MC, Sun PF, Tseng YH: Clp and RpfF up-regulate transcription of pelA1 gene encoding the major pectate lyase in Xanthomonas campestris pv. campestris. J Agric Food Chem. 2009, 57 (14): 6207-6215. 10.1021/jf900701n.

    PubMed  CAS  Google Scholar 

  94. Meyer F, Goesmann A, McHardy AC, Bartels D, Bekel T, Clausen J, Kalinowski J, Linke B, Rupp O, Giegerich R, et al: GenDB - an open source genome annotation system for prokaryote genomes. Nucleic Acids Res. 2003, 31 (8): 2187-2195. 10.1093/nar/gkg312.

    PubMed  CAS  PubMed Central  Google Scholar 

  95. Blom J, Albaum SP, Doppmeier D, Puhler A, Vorhölter FJ, Zakrzewski M, Goesmann A: EDGAR: A software framework for the comparative analysis of prokaryotic genomes. BMC Bioinformatics. 2009, 10 (1): 154-10.1186/1471-2105-10-154.

    PubMed  PubMed Central  Google Scholar 

  96. Pieretti I, Royer M, Barbe V, Carrere S, Koebnik R, Cociancich S, Couloux A, Darrasse A, Gouzy J, Jacques MA, et al: The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae. BMC Genomics. 2009, 10: 616-10.1186/1471-2164-10-616.

    PubMed  PubMed Central  Google Scholar 

  97. Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, et al: Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. J Bacteriol. 2005, 187 (21): 7254-7266. 10.1128/JB.187.21.7254-7266.2005.

    PubMed  CAS  PubMed Central  Google Scholar 

  98. Salzberg SL, Sommer DD, Schatz MC, Phillippy AM, Rabinowicz PD, Tsuge S, Furutani A, Ochiai H, Delcher AL, Kelley D, et al: Genome sequence and rapid evolution of the rice pathogen Xanthomonas oryzae pv. oryzae PXO99A. BMC Genomics. 2008, 9: 204-10.1186/1471-2164-9-204.

    PubMed  PubMed Central  Google Scholar 

  99. Ochiai H, Inoue V, Takeya M, Sasaki A, Kaku H: Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity. Jarq-Jpn Agr Res Q. 2005, 39 (4): 275-287.

    CAS  Google Scholar 

  100. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B: The Carbohydrate-Active EnZymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res. 2009, 37 (Database issue): D233-D238.

    PubMed  CAS  PubMed Central  Google Scholar 

  101. Sambrook H, Fritsch EF, Maniatis T: Molecular cloning: a laboraratory manual. 1989, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 2

    Google Scholar 

  102. Wilder JA, Cowdery JS, Ashman RF: The influence of lipopolysaccharide content on the apparent B cell stimulating activity of anti-μ preparations. J Immunol Methods. 1988, 110 (1): 63-68. 10.1016/0022-1759(88)90083-X.

    PubMed  CAS  Google Scholar 

  103. Silswal N, Singh AK, Aruna B, Mukhopadhyay S, Ghosh S, Ehtesham NZ: Human resistin stimulates the pro-inflammatory cytokines TNF-alpha and IL-12 in macrophages by NF-kappaB-dependent pathway. Biochem Biophys Res Commun. 2005, 334 (4): 1092-1101. 10.1016/j.bbrc.2005.06.202.

    PubMed  CAS  Google Scholar 

  104. Warm E, Laties GG: Quantification of hydrogen peroxide in plant extracts by the chemoluminescence reaction with luminol. Phytochem. 1982, 21: 827-831. 10.1016/0031-9422(82)80073-3.

    CAS  Google Scholar 

  105. Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962, 15: 473-497. 10.1111/j.1399-3054.1962.tb08052.x.

    CAS  Google Scholar 

Download references


We gratefully acknowledge Dorothee Steinmann for providing the X. campestris pv. campestris mutant strain B100-Bac2. Also, we want to thank Dr. Bruno Moerschbacher from the Institut für Biochemie und Biotechnologie in Münster, Germany for the kind permission to use his HPAEC system. At Bielefeld University, the project benefitted from work carried out by, Julia Voß, Sergej Wendler, Anna Köpfer, and Tim Steffens. Jannis Harfmann provided supportive transcriptomics data. Completing the project successfully benefited substantially from oxidative burst measurements carried out by Barbara Samenfeld.

This work was financially supported by the BMBF program “GenoMik Plus”. We acknowledge support of the publication fee by Deutsche Forschungsgemeinschaft and by the Open Access Publication Funds of Bielefeld University.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Frank-Jörg Vorhölter.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

FJV has performed genomic analyses, compiled the experimental results, and wrote the main part of the manuscript. HGW initially suggested the study, provided genetic constructs, and analyzed the pectate lyase activity and its effect on the HR of X. campestris pv. campestris strains on C. annuum. HS carried out in large part the OGA-related analyses and composed an early draft of the manuscript. VKS characterized the isolated pectate fragments by HPAE chromatography. KM carried out oxidative burst measurements with suspension cell cultures of the non-host plant N. tabacum. HK supervised experiments carried out by HS. AP provided infrastructure and advice, in particular related to the genes of the TonB system. KN supervised the whole project, and provided part of the manuscript’s discussion section. All authors read and approved the final manuscript.

Electronic supplementary material

Additional file 1: Multiple alignment of Xanthomonas exbD2 gene products.(PDF 12 KB)


Additional file 2: Figure displaying the recovery of extracellular pectate lyase activities in complemented X. campestris pv. campestris strains originally deficient in genes of the TonB system.(PDF 232 KB)

Additional file 3: Table S1 with pectate lyase activity in X. campestris pv. campestris and E. coli strains.(PDF 11 KB)


Additional file 4: Figure displaying oxidative burst reactions in heterologous N. tabacum cell suspension cultures upon elicitation with supernatants of X. campestris pv. campestris cultures deficient in genes of the TonB system.(PDF 29 KB)

Additional file 5: Table S2 with genes of pectin-degrading enzymes in X. campestris pv. campestris B100.(PDF 12 KB)

Authors’ original submitted files for images

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Vorhölter, FJ., Wiggerich, HG., Scheidle, H. et al. Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases. BMC Microbiol 12, 239 (2012).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: