Strain or plasmid | Relevant genotype and/or description | Source or reference |
---|---|---|
X. campestrispv. campestris strains | ||
B100 | Wild-type, Smr | [46] |
B100-6.01 | Control strain, carrying ΩKm(cat) in intergenic region flanked by tonB1 and exbB1, Smr, Kmr | [64] |
B100-5.05 | tonB1-deficient mutant, Smr, Kmr | [64] |
B100-7.03 | exbB1-deficient mutant, Smr, Kmr | [64] |
B100-9.01 | exbD1-deficient mutant, Smr, Kmr | [64] |
B100-11.03 | exbD2-deficient mutant, Smr, Kmr | [64] |
B100-Bac2 | Bacitracin-resistant spontaneous mutant of B100, unable to produce polysaccharides, Smr | D. Steinmann, CeBiTec culture collection |
E. coli strain | ||
XL1Blue | recA1, thi, supE44, lac, [F’proAB lacIq, lacZΔM15, Tn10(Tcr)] | [89] |
Plasmids | ||
pUC6S | lacZα, Apr | [90] |
pBCKS+ | pUC19, lacZ, Cmr | Stratagene |
pBCSK+ | pUC19, lacZ, Cmr | Stratagene |
pMS246 | pSVB30, aacC1, Gmr | [91] |
pHGW31 | pHIP, aacC1ΔBglII, Gmr | [64] |
pHGW241 | pHGW31, tonB1, Gmr | [64] |
pHGW242 | pHGW31, exbB1, Gmr | [64] |
pHGW243 | pHGW31, exbD1, Gmr | [64] |
pHGW244 | pHGW31, exbD2, Gmr | [66] |
pIJ3051 | pLAFRI-based cosmid carrying 27.9 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Tcr | [39] |
pHGW260 | pHGW31, 11.1 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Gmr | This study |
pHGW261 | pBCKS+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cmr | This study |
pHGW262 | pBCSK+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cmr | This study |
pHGW267 | pUC6S, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260 expressed from aacC1 Pout promoter, Apr, Gmr | This study |