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Table 1 Bacterial strains and plasmids used in this study

From: Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases

Strain or plasmid

Relevant genotype and/or description

Source or reference

X. campestrispv. campestris strains

B100

Wild-type, Smr

[46]

B100-6.01

Control strain, carrying ΩKm(cat) in intergenic region flanked by tonB1 and exbB1, Smr, Kmr

[64]

B100-5.05

tonB1-deficient mutant, Smr, Kmr

[64]

B100-7.03

exbB1-deficient mutant, Smr, Kmr

[64]

B100-9.01

exbD1-deficient mutant, Smr, Kmr

[64]

B100-11.03

exbD2-deficient mutant, Smr, Kmr

[64]

B100-Bac2

Bacitracin-resistant spontaneous mutant of B100, unable to produce polysaccharides, Smr

D. Steinmann, CeBiTec culture collection

E. coli strain

XL1Blue

recA1, thi, supE44, lac, [F’proAB lacIq, lacZΔM15, Tn10(Tcr)]

[89]

Plasmids

pUC6S

lacZα, Apr

[90]

pBCKS+

pUC19, lacZ, Cmr

Stratagene

pBCSK+

pUC19, lacZ, Cmr

Stratagene

pMS246

pSVB30, aacC1, Gmr

[91]

pHGW31

pHIP, aacC1ΔBglII, Gmr

[64]

pHGW241

pHGW31, tonB1, Gmr

[64]

pHGW242

pHGW31, exbB1, Gmr

[64]

pHGW243

pHGW31, exbD1, Gmr

[64]

pHGW244

pHGW31, exbD2, Gmr

[66]

pIJ3051

pLAFRI-based cosmid carrying 27.9 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Tcr

[39]

pHGW260

pHGW31, 11.1 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Gmr

This study

pHGW261

pBCKS+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cmr

This study

pHGW262

pBCSK+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cmr

This study

pHGW267

pUC6S, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260 expressed from aacC1 Pout promoter, Apr, Gmr

This study