Transcriptomic analysis of Clostridium thermocellumATCC 27405 cellulose fermentation
© Raman et al; licensee BioMed Central Ltd. 2011
Received: 13 July 2010
Accepted: 14 June 2011
Published: 14 June 2011
The ability of Clostridium thermocellum ATCC 27405 wild-type strain to hydrolyze cellulose and ferment the degradation products directly to ethanol and other metabolic byproducts makes it an attractive candidate for consolidated bioprocessing of cellulosic biomass to biofuels. In this study, whole-genome microarrays were used to investigate the expression of C. thermocellum mRNA during growth on crystalline cellulose in controlled replicate batch fermentations.
A time-series analysis of gene expression revealed changes in transcript levels of ~40% of genes (~1300 out of 3198 ORFs encoded in the genome) during transition from early-exponential to late-stationary phase. K-means clustering of genes with statistically significant changes in transcript levels identified six distinct clusters of temporal expression. Broadly, genes involved in energy production, translation, glycolysis and amino acid, nucleotide and coenzyme metabolism displayed a decreasing trend in gene expression as cells entered stationary phase. In comparison, genes involved in cell structure and motility, chemotaxis, signal transduction and transcription showed an increasing trend in gene expression. Hierarchical clustering of cellulosome-related genes highlighted temporal changes in composition of this multi-enzyme complex during batch growth on crystalline cellulose, with increased expression of several genes encoding hydrolytic enzymes involved in degradation of non-cellulosic substrates in stationary phase.
Overall, the results suggest that under low substrate availability, growth slows due to decreased metabolic potential and C. thermocellum alters its gene expression to (i) modulate the composition of cellulosomes that are released into the environment with an increased proportion of enzymes than can efficiently degrade plant polysaccharides other than cellulose, (ii) enhance signal transduction and chemotaxis mechanisms perhaps to sense the oligosaccharide hydrolysis products, and nutrient gradients generated through the action of cell-free cellulosomes and, (iii) increase cellular motility for potentially orienting the cells' movement towards positive environmental signals leading to nutrient sources. Such a coordinated cellular strategy would increase its chances of survival in natural ecosystems where feast and famine conditions are frequently encountered.
Among cellulolytic microorganisms, the anaerobic, thermophilic, Gram-positive bacterium, Clostridium thermocellum displays one of the fastest growth rates on crystalline cellulose [1, 2]. This native cellulolytic organism encodes a repertoire of carbohydrate active enzymes (CAZymes) for degradation of plant cell wall polysaccharides, which are assembled in large enzyme complexes, termed cellulosomes, on the cell surface [3, 4]. C. thermocellum is thus capable of both deconstructing crystalline cellulose into oligomeric cello-oligosaccharides and fermenting the hydrolysis products directly to ethanol and other organic acids, consequently minimizing or eliminating the need for external addition of non-native hydrolytic enzymes. Elimination of a separate cellulase-production step is economically advantageous for industrial cellulosic ethanol production processes [5, 6]. C. thermocellum is therefore an attractive candidate microorganism for consolidated bioprocessing of lignocellulosic biomass to biofuels.
Several past studies have investigated the expression and regulatory nature of approximately two dozen selected genes encoding cellulosomal catalytic and structural components in C. thermocellum [7–12]. Dror et al. reported growth-rate dependent regulation of cellulosomal endoglucanases (celB, celD, celG) and the major processive endoglucanase celS [7, 9]. A growth-rate dependent variation of mRNA levels was also reported for the cellulosome scaffoldin genes cipA and the anchor genes olpB and orf2p but not sdbA . In continuous cultures studies, Zhang and Lynd, using an ELISA method, suggested cellulase synthesis in C. thermocellum to be regulated by a catabolite repression type mechanism . Sparling, Levin and colleagues have investigated the gene expression and enzymatic activities of several proteins involved in pyruvate metabolism and fermentation [13, 14].
A draft assembly of the C. thermocellum genome sequence became available in 2003, which was subsequently completed and the genome was closed in 2006. This paved the way for whole-genome gene and protein expression studies. We previously reported the construction and evaluation of a whole genome oligo-nucleotide microarray with probes representing ~95% of the open reading frames based on the draft assembly of the C. thermocellum genome sequence . Microarrays are invaluable research tools that provide comprehensive information on the underlying molecular mechanisms for cellular behavior, states and transcriptional regulation. In this study, microarray technology was used to investigate the temporal changes in gene expression associated with fermentation of crystalline cellulose by C. thermocellum.
Overall, the gene expression patterns revealed a coordinated response by C. thermocellum to conditions of altering substrate availability during cellulose batch fermentations. C. thermocellum modulates the composition of cellulosomes released into the environment in stationary phase and enhances signal transduction, chemotaxis mechanisms probably for sensing of substrate gradients resulting from the action of cell-free cellulosomes. C. thermocellum also increases expression of genes involved in cellular motility function, potentially to orient the movement of cells towards available nutrient sources in the environment. Such a coordinated cellular strategy should increase its chances of survival under conditions akin to feast and famine that are frequently encountered in natural ecosystems. To our knowledge, this is the first study looking at the transcriptional response of C. thermocellum at a global level and provides the foundation for future research using natural biomass as growth substrates.
C. thermocellum ATCC 27405 wild-type strain was a gift from Prof. Herb Strobel at the University of Kentucky, Lexington, KY. Batch fermentations were conducted in 3 L BioStat B jacketed glass fermentors (Sartorius Stedim Biotech, Bohemia, NY) using a 2 L working volume of MTC medium (mineral salt medium containing 1 g/L yeast extract; ) at 58°C and 300 rpm, with pH controlled at 7.0 using 3N NaOH. Fermentors with medium containing only the carbon substrate, 5 g/L crystalline cellulose (Avicel® PH105, FMC Biopolymer, Philadelphia, PA), were sparged with ultra-high purity nitrogen and vigorously agitated overnight, followed by addition of the remaining medium components and sparged for an additional 2-3 hrs with nitrogen gas. A 10% v/v inoculum of overnight (16-20 hrs) 5 g/L Avicel® bottle cultures was used to inoculate the fermentors and the gas inlet/exhaust lines were clamped post inoculation.
Protein and metabolite analysis
Well-mixed 2 mL aliquots of cultures were harvested at regular intervals and centrifuged quickly to separate into pellet and supernatant samples for protein analysis of pellet fractions and HPLC analysis of extracellular metabolites, respectively. Cell growth was monitored based on increase in protein content within the total solids present in the pellet fraction, including the Avicel® substrate . Briefly, the solid pellet was washed with de-ionized water and the cells were lysed using 0.2N NaOH/1% w/v SDS solution, cell debris were pelleted and removed, and protein concentration in the clear supernatant was estimated using the bicinchoninic acid protein assay (Pierce Chemical, Rockford, IL). Metabolite analysis was performed using a LaChrom Elite system (Hitachi High Technologies America, Inc., Pleasanton, CA) equipped with a refractive index detector (Model L-2490). Metabolites were separated at a flow rate of 0.5 mL/min in 5 mM H2SO4 using an Aminex HPX-87H column (Bio-Rad Laboratories, Inc., Hercules, CA).
RNA isolation and microarray analysis
Fermentation samples for RNA isolation were harvested by spinning down ~30 mL culture in 50 mL Oak Ridge tubes at 8000 rpm and 4°C for 10-15 mins and the supernatant was discarded. The solid pellet fraction containing cells and any residual Avicel® was resuspended in 1 mL of TRIzol (Invitrogen, Carlsbad, CA), flash frozen in liquid nitrogen and stored at -80°C until further use. Total RNA was extracted from the cell pellets as follows. Briefly, the frozen cell solution in TRIzol was thawed on ice and the cell solution (~1 mL) was added to a 2 mL tube containing 1 mL of 0.1 mm glass beads (BioSpec Products, Bartlesville, OK) ashed at 250°C overnight. Cells were lysed by rapid agitation of the tubes at 6500 rpm for 1 min in three 20s-On/20s-Off cycles using the Precellys® bead beater (Bertin Technologies, France). Subsequently, the cell lysate (~0.8 mL) in TRIzol was phase separated by addition of 200 μL chloroform and the RNA was precipitated by addition of 500 μL 100% isopropanol. The precipitated RNA pellet was washed with 1 mL of 75% ethanol and resuspended in 100 μL of RNase-free water. Any contaminating DNA was digested by in-solution DNase-I (Qiagen, Valencia, CA) treatment and the RNA sample was cleaned using the RNeasy mini kit (Qiagen, Valencia, CA) as per manufacturer's instructions. The 6 hr time-point RNA sample was used as the reference and all other time-point samples (8, 10, 12, 14, 16 hr) were compared to the reference in cDNA/cDNA arrays. For each time-point comparison, equal amount of the extracted total RNA samples was labeled with Cy3-dUTP/Cy5-dUTP fluorescent dyes (GE Healthcare, Piscataway, NJ), mixed and hybridized onto custom oligo-arrays in dye swap experiments as described earlier  and microarray slides were scanned in ScanArray Express scanner (Perkin Elmer, Waltham, MA).
Microarray construction and statistical data analysis
Microarrays containing 2980 unique and 10 group 70-mer oligonucleotide probes representing ~97% of the 3163 Open Reading Frames (ORFs) in the draft assembly of C. thermocellum ATCC 27405 were constructed as described earlier . The probe sequences were later compared to the completed genome sequence using reciprocal BLAST analysis and assigned new ORF numbers. Based on the comparison, 79 probes which did not have any BLAST hits and 108 probes that only had partial hits to annotated ORFs in the closed genome were either excluded or marked-up during downstream data analysis. Signals were quantified in ImaGene version 6.0 (BioDiscovery Inc., El Segundo, CA) and statistical data analysis was conducted using JMP Genomics software (SAS Institute Inc., Cary, NC). The array signal intensities were background-corrected, log2-transformed and data for duplicated probes on the arrays were averaged and normalized using the Data-Standardize method. Low-quality array data were discarded based on scatter plots, correlation coefficients, principal component analysis and other quality control criteria. One-way ANOVA analysis was conducted with the 6 hr samples as the control at a False Discovery Rate of 2% (P-value < 0.01) to identify differentially expressed genes of statistical significance. Genes significantly up- or down-regulated in at least one time-point comparison were analyzed in TIGR MeV 4.5 software  to identify similar temporal trends in gene expression using average-linkage hierarchical or K-means clustering methods.
Results and Discussion
Total RNA was extracted from the cell pellets and the reverse transcribed cDNA was hybridized to oligo-arrays containing duplicated probes representing ~90% of the annotated ORFs in C. thermocellum ATCC27405 genome. Dual-channel dye swap experimental design was used to analyze the time-course of gene expression during cellulose fermentation using the 6 hr sample as the reference, to which all other samples were compared. The entire microarray dataset has been deposited in NCBI's Gene Expression Omnibus (GEO, ) database under accession number GSE29554 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE29554). Data analysis revealed over ~1300 genes that were differentially expressed with statistical significance in at least one time point comparison. This represents ~40% of 3198 ORFs in C. thermocellum showing significant changes in gene expression over the course of cellulose fermentation. Gene expression ratios estimated by microarray methods displayed high correlation with those measured by quantitative RT-PCR, for five representative genes across two different time-points, with an R-value of 0.92 (Additional file 1). Hierarchical clustering and principal component analysis of sample datasets revealed clustering of the 6 h exponential sample distinctly from the rest of the time points. Among these were three branches corresponding to late exponential phase (8, 10 h), transition to stationary phase at 12 h and late stationary phase samples (14, 16 h) (data not shown).
The operon structure prediction for C. thermocellum ATCC 27405 by DOOR database (; http://csbl1.bmb.uga.edu/OperonDB/) was used to estimate the correlation for co-regulation of genes in contiguous regions of the genome within predicted operons. Overall there was significant correlation between the total number of genes and the number of genes differentially expressed in a predicted operon that exhibited co-regulated patterns in expression with either concerted increase (9 operons, R-value 0.97) or decrease (30 operons, R-value 0.81-0.96) in transcript levels (data not shown). Examples included two large predicted operons, Cthe0480-0496 (17 ORFs) and Cthe2908-2928 (21 ORFs), in which 14 and 13 genes were differentially expressed, respectively. The former operon, containing several genes involved in flagellar biosynthesis, pili assembly, chemotaxis and signal transduction, displayed an increasing trend in expression while the latter operon, containing genes encoding several large and small ribosomal subunit proteins, showed a progressively decreasing trend in expression over the course of cellulose fermentation.
Central metabolism and mixed-acid fermentation genes
Upstream of phosphoenolpyruvate
The pentose phosphate pathway is important for production and supply of key intermediates involved in the synthesis of nucleotides and aromatic amino acids. The C. thermocellum genome encodes several enzymes in the non-oxidative branch of the Pentose Phosphate (PP) pathway including ribulose-5-P isomerase (Cthe2597) and ribulose-5-P epimerase (Cthe0576) (Figure 4, Additional file 4). During cellulose fermentation, the epimerase gene was downregulated by up to 2-fold in stationary phase, while the isomerase gene was expressed at high levels throughout the course of the fermentation. C. thermocellum also has two pairs of contiguous genes encoding transketolases (Cthe2443-44 and Cthe2704-05) which catalyze several reactions in the PP pathway, of which only the Cthe2704-05 pair shows maximal expression during cellulose fermentation (Figure 4). Sequence homology-based annotation has however not revealed a transaldolase in C. thermocellum.
Downstream of phosphoenolpyruvate
Similar to glycolytic enzymes, a majority of the genes involved in conversion of phosphoenolpyruvate to pyruvate and mixed-acid fermentation of pyruvate to various organic acids and ethanol were downregulated during stationary phase of C. thermocellum growth on cellulose (Figure 4, Additional file 5: Expression of genes downstream of PEP). Several Gram-positive organisms, including representatives in the Clostridial species such as C. phytofermentans and C. cellulolyticum, encode genes for pyruvate phosphate kinase, which catalyzes the conversion of PEP to pyruvate, but genome annotation has not identified a homologous gene in C. thermocellum. An ORF recently suggested as a putative pyruvate kinase homologue, Cthe1955 , had relatively minimal expression during cellulose fermentation (Figure 4). C. thermocellum however has two copies of the gene encoding pyruvate phosphate dikinase (Cthe1253 and Cthe1308), which catalyzes the reverse reaction for conversion of pyruvate to PEP. This enzyme is suggested to play an anabolic role in gluconeogenesis and consistent with this, Cthe1253 is upregulated in stationary phase during growth on cellulose. Sparling and colleagues have proposed an alternate route for conversion of PEP to pyruvate via oxaloacetate and malate (Figure 4; Sparling, personal communication). Genes encoding all three enzymes in this alternative route, the gluconeogenic PEP carboxykinase (Cthe2874), malate dehydrogenase (Cthe0345), and malic enzyme (Cthe0344), were expressed at high levels, suggesting that this putative pathway is active in C. thermocellum during growth on cellulose.
C. thermocellum contains two clusters of genes (Cthe2390-2393 and Cthe2794-2797) encoding the gamma, delta, alpha and beta subunits, respectively, of the thiamine-pyrophosphate (TPP) dependent pyruvate ferredoxin oxidoreductase (POR) which catalyzes the oxidation of pyruvate to acetyl-CoA. While all the genes in the Cthe2390-2393 cluster displayed maximal expression during cellulose fermentation, only a single gene in the Cthe2794-2797 cluster, encoding the TPP-binding beta subunit of the POR protein complex (Cthe2797), had high transcript levels which decreased in stationary phase (Figure 4). This is in contrast to studies by Sparling and colleagues who reported expression of Cthe2794-97 transcripts by RT-PCR during log phase of growth on alpha-cellulose with only weak expression of the Cthe2390-93 cluster . However, the observed trends in gene expression may be due to differences in culture conditions between the two studies. While qPCR analysis was done with batch cultures in Balch tubes with no pH control , microarray analysis in this study was conducted with samples from controlled fermentations in bioreactors with pH control.
C. thermocellum encodes several genes involved in the mixed-acid fermentation pathways for conversion of pyruvate to organic acids (lactate, formate, acetate) and ethanol (Additional file 5, Expression of genes downstream of PEP). These include two putative lactate dehydrogenase genes (ldh; Cthe0345, Cthe1053) involved in conversion of pyruvate to lactate. Previous studies have reported detecting LDH activity in cell extracts of C. thermocellum [14, 25, 26] and RT-PCR analysis has shown expression of both ldh genes during cellulose batch and continuous fermentations [11, 13]. LDH, Cthe1053, cloned and expressed in E. coli, has been functionally characterized and demonstrated to oxidize pyruvate to lactate . In this study, the Cthe1053 gene displayed low expression and lactate was not detected during cellulose fermentation. Although another gene annotated as ldh (Cthe0345) was expressed at high levels, this may be related to the participation of the encoded enzyme as a malate dehydrogenase in the alternate route for conversion of PEP to pyruvate, as discussed earlier (Figure 4). Pyruvate formate lyase (pfl) catalyzes the conversion of pyruvate to formate, along with the formation of acetyl-CoA. Sparling et al, reported formate synthesis in C. thermocellum via this pathway with detection of transcripts for pfl (Cthe0505) and the pfl activating enzyme (Cthe0506) by RT-PCR . In this study, two out of four putative pfl activating enzymes (Cthe0506, Cthe0647) were expressed at relatively high levels during cellulose fermentation (Additional file 5; data not available for pfl, Cthe0505). However, formate was not detectable in the culture supernatant consistent with other previous reports [25, 28].
Acetyl-CoA is further catabolized to acetate with generation of ATP or to ethanol with reoxidation of NADH. C. thermocellum encodes an NADH-dependent aldehyde dehydrogenase (aldH, Cthe2238), which catalyzes the conversion of acetyl-CoA to acetaldehyde, and several iron-containing alcohol dehydrogenases (Cthe0101, Cthe0394 [adhY] and Cthe2579 [adhZ]) for alcohol synthesis from acetaldehyde; also encoded is a bi-functional acetaldehyde/alcohol dehydrogenase (Cthe0423, adhE) which catalyzes the direct conversion of acetyl-CoA to ethanol (Figure 4). AdhE has been proposed to be a key enzyme for ethanol synthesis in C. thermocellum and transcription of adhE, adhY and adhZ has been confirmed by RT-PCR analysis in cellobiose and cellulose cultures of C. thermocellum [11, 19]. In this study, the aldH gene showed increased expression during stationary phase while the three adh genes, Cthe0394, Che0423 and Cthe0101, were actively expressed during cellulose batch fermentation with the latter showing decreased expression in stationary phase (Figure 4, Additional file 5).
Acetyl-CoA is indirectly converted to acetate via acetyl-phosphate through the action of two enzymes, encoded by the contiguous genes, phosphotransacetylase (pta, Cthe1029) and acetate kinase (ack, Cthe1028), with the generation of one ATP per acetate molecule. The reverse reaction for direct conversion of acetate to acetyl-CoA utilizes ATP and is catalyzed by acetyl-CoA synthetase (acs, Cthe0551). Previous studies have confirmed the expression of acetate kinase through RT-PCR  and enzyme activity measurements . In this study, both pta and ack genes were expressed at low levels which further decreased in stationary phase; whereas, the acs gene was expressed at relatively higher levels over the entire course of the fermentation (Figure 4, Additional file 5). The observed gene expression patterns are consistent with our metabolite analysis of the fermentation medium, which showed decreasing acetate-to-ethanol molar ratios during entry into stationary phase (Figure 1). Several genes involved in conversion of pyruvate to other intermediate metabolites such as α-ketoglutarate, which is a building block for amino acid and nucleic acid biosynthesis, also showed high level of expression during active growth but lowered levels in stationary phase (Additional file 5), possibly due to reduced metabolic need under slow growth and nutrient-limited conditions.
Energy generation and redox balance
Overall, the genes involved in maintaining the intracellular redox conditions and cellular energy production systems belonged to clusters C2, C4 and C6 and were downregulated with decreasing growth rate over the course of cellulose batch fermentation (Additional file 6, Expression of genes involved with energy generation and redox balance).
Cellulosomal and non-cellulosomal carbohydrate active enzymes
In C. thermocellum, cellulases and other polysaccharide degrading enzymes are assembled together in large protein complexes, termed the cellulosome, on the cell-surface. The cellulosome complex has a primary scaffoldin protein, CipA, containing 9 type-I cohesin-modules and catalytic subunits, each containing a complementary type-I dockerin module, interact strongly with the cohesin module for assembly onto the scaffoldin. CipA with bound enzymes is in turn attached to the cell surface via interaction between the CipA-borne type-II dockerin and type-II cohesins of the cell wall anchor proteins. During growth on insoluble substrates, the cells are tightly attached to the substrate via the carbohydrate binding module (CBM) borne by CipA and many catalytic subunits of the cellulosomes forming a cell-cellulosome-carbohydrate complex.
Structural cellulosome components include the scaffoldin CipA (Cthe3077) and seven anchor proteins, five containing type-II cohesins (Cthe1307/SdbA, Cthe 3078/OlpB, Cthe3079/Orf2p, Cthe0735 and Cthe0736) and two containing type-I cohesin (Cthe3080/OlpA, Cthe0452). Among these, genes encoding CipA, Orf2p, OlpB and OlpA exhibited maximal expression during cellulose fermentation (Additional file 7). Expression of orf2p increased by up to 2-fold over the course of the batch fermentation in agreement with Dror et al. who reported an inverse correlation between growth rate and mRNA levels of the anchor genes, olpB, orf2p and the scaffoldin cipA . However, in this study, expression levels of cipA did not change significantly during batch growth and olpB displayed a moderate decrease in expression in stationary phase (Figure 6, Additional file 7).
Newcomb and Wu  characterized a LacI family transcriptional regulator, GlyR3 (Cthe2808), in C. thermocellum that was shown to regulate the expression of two non-cellulosomal CAZymes, a GH16 family lichinase (licA, Cthe2809) and a GH5 family cellulase (celC, Cthe2807), all encoded together in the putative celC operon, Cthe2807-2809. During cellulose fermentation, genes in this operon displayed relatively little expression in exponential phase but their transcript levels continually increased with maximal expression of >3-fold in stationary phase (Figure 7, Additional file 7). Mishra et al. also observed a similar expression pattern during cellobiose fermentation in which celC transcripts were detected exclusively in early stationary phase after cessation of growth . Differential expression of the operon in the absence of laminaribiose, the identified GlyR3 inducer , suggests that other cellulose-derived oligosaccharides may also act as inducers or other regulatory mechanisms may be involved.
Recent evidence suggests the possible role of membrane-associated anti-sigma factors in extracellular carbohydrate-sensing and CAZyme gene regulation in C. thermocellum. Kahel-Raifer et al. identified several putative bicistronic operons in the C. thermocellum genome, each operon encoding an RsgI-like anti-σ factor and a putative alternative sigma factor σI (SigI) and proposed a regulatory model, wherein RsgI senses the presence of biomass components in the extracellular medium via its CBM domain while SigI mediates the intracellular activation of appropriate CAZyme genes that are necessary for hydrolysis of the polysaccharide substrate, in response to the transmitted signal . In this study, three of the σI encoding genes (Cthe0058, Cthe0268, Cthe0403) that are associated with anti-σI -like genes bearing cellulose-binding CBM3 domains were all upregulated, with Cthe0268 showing ~5-fold increased expression, during later stages of the cellulose fermentation (Additional file 8: Expression of genes involved in carbohydrate sensing and CAZyme regulation). The observed pattern in expression of CBM3-related σI genes, i.e., their increased expression in stationary phase, seems to differ from the regulatory model proposed by Kahel-Raifer et al., who suggested induced expression of sigma factor in the presence of the polysaccharide substrate . This is probably explained by the presence of residual Avicel in the stationary phase or perhaps suggests the involvement of additional mechanisms, such as growth rate, in the regulation of sigI genes. However, several genes encoding GH9 family cellulases (Cthe0043/CelN, Cthe0413/CbhA, Cthe0543/CelF, Cthe0745/CelW, Cthe2812/CelT etc.) were also upregulated with peak expression in early-to-late stationary phase (Additional file 7) and are potentially part of SigI regulon in C. thermocellum.
Several of the cellulosomal CAZymes with significant upregulated expression in later stages of fermentation belonged to GH families with hydrolytic capabilities specializing in degradation of non-cellulolytic substrates - for example, GH26 family mannanases (Cthe0032, manA/Cthe2811), GH74 family xyloglucanase (xghA/Cthe1398), Family 11 pectate lyase (Cthe0246), GH10 and GH11 family xylanases (xynY/Cthe0912, xynA-U/Cthe2972), bi-functional CelH (Cthe1472) containing GH5 and GH26 domains etc. (Figure 6, Additional file 7). Given that C. thermocellum releases the cellulosomes in stationary phase , it is likely that the increased expression of non-cellulolytic GH family enzymes during the latter part of growth is aimed towards enriching this population of enzymes in the free cellulosomes to aid in exposing the preferred substrate of cellulose from untapped resources in the cellular vicinity. Increase in expression of the two serine protease inhibitor components (Cthe0190, Cthe0191) may serve to protect the free cellulosomes from proteolytic degradation.
Cellodextrin transport-related genes
Ten percent of the ORFs in the C. thermocellum genome encode proteins that are involved in transport of oligosaccharides, amino acids, inorganic and metal ions, co-factors etc. C. thermocellum has been reported to use ABC-type systems for transport of oligosaccharides derived from cellulose hydrolysis . Recently, Shoham and colleagues characterized several ABC sugar binding proteins in C. thermocellum (CbpA, Cthe0393; CbpB, Cthe1020; CbpC, Cthe2128; CbpD, Cthe2446) based on their affinity to glucose and G2-G5 cello-oligosaccharides . In this study, genes in contiguous regions (Cthe0391-0393 and Cthe1019-1020) encoding CbpA and CbpB proteins with binding affinities to G3 and G2-G5 beta-1,4-glycans, respectively, and Cthe1862, encoding another sugar binding protein of unknown specificity, were expressed at high levels throughout the course of cellulose fermentation (Figure 4). This observation is consistent with the study by Zhang and Lynd demonstrating the preference of C. thermocellum for importing 4-glucose-unit chains during growth on cellulose. The bioenergetic implications of importing long cellodextrins are two-fold, (i) from reduced cost of transport as only one-ATP molecule is needed per transport event irrespective of the chain length and (ii) additional energetic advantage from phosphorolytic cleavage of the imported oligosaccharides .
Chemotaxis, signal transduction and motility genes
The majority of genes involved in flagellar- and pili-based cell motility and chemotaxis-based signal transduction mechanisms displayed an increasing trend in expression over the course of cellulose fermentation. Approximately, 81% of all differentially expressed (DE) genes belonging to COG category N (motility-related) and 64% of all DE genes belonging to COG category T (signal transduction) were grouped to clusters C1, C3 and C5, which contain genes showing increased expression in various stages of growth (Figures 2, 3). In C. thermocellum, many of the genes involved in flagellar biosynthesis/assembly and two-component signal transduction are clustered in a genomic region containing ORFs ranging from Cthe0462-0496. Several genes in this region, within putative operons Cthe0462-0464 (all 3 genes) and Cthe0480-0496 (14 out of 17 genes), were coordinately upregulated during cellulose fermentation. Many genes in another genomic region, Cthe1100-1107, encoding fimbrial assembly and type II secretion system proteins, also showed increased expression by up to 3-fold during growth. These results suggest potentially increased motility of C. thermocellum during later stages of the fermentation. This is in contrast to reports of decreased expression of flagellar and chemotaxis genes in solventogenic members of the clostridia, C. beijerinckii  and C. acetobutylicum  during shift from acidogenic to solventogenic phase or at the onset of sporulation, respectively. In C. thermocellum, upregulated expression of motility- and chemotaxis-related genes under conditions of low substrate availability, suggest a cellular strategy oriented towards enhancing the ability of cells to sense the environment and appropriately respond to the ambient signals through activation of the cellular motility systems.
Due to its native cellulolytic capability and ability to ferment cellulose hydrolysis products directly to ethanol, Clostridium thermocellum is an attractive candidate microorganism for consolidate bioprocessing of plant biomass to biofuels. Understanding the microbial physiology associated with cellulase synthesis, cellulose degradation, and cellular growth is vital to identifying genetic targets for manipulation and strain improvement. In this study, we probed C. thermocellum gene expression during the course of cellulose fermentation using whole genome microarray technology. Time course analysis of gene expression coupled with clustering of genes with similar temporal patterns in expression revealed an overall decrease in metabolic potential of the organism over the course of the fermentation. Several genes involved in energy production, translation, glycolysis and amino acid, nucleotide and coenzyme metabolism displayed a progressively decreasing trend in gene expression. In comparison, genes involved in cell structure and motility, chemotaxis, signal transduction, transcription and cellulosomal genes displayed an increasing trend in gene expression.
While growth-rate related changes in cell growth and metabolism genes have been well documented, the increasing trend in expression of CAZyme genes, especially when the overall energy and protein synthesis capacity of the cells is at its minimal throughput in the stationary phase is rather surprising. This might denote a cellular strategy to channel the available resources towards the cellulolytic machinery, thereby increasing its chances of finding new sources of nutrition. During active growth, the cellulosome complex is tightly adhered to the cell surface and also to the solid substrate forming a tri-complex between cells, cellulosome and cellulose. But, when growth begins to slow-down, C. thermocellum is known to release the cellulosomes into the culture medium , perhaps through sensing the decreasing supply of oligosaccharides. The released cellulosomes could then act as 'deployed soldiers in the battlefield,' whereby they are free to diffuse and 'hunt' for alternate sources of nutrients in the environment. Increasing the expression of non-cellulolytic enzymes and thus modulating the composition of the released cellulosomes would enhance the chances for successfully 'un-wrapping' the preferred substrate of cellulose from other plant polysaccharides such as hemicellulose and pectin. However, it is not yet known if there are distinct differences in the composition of the attached vs the detached cellulosomes in C. thermocellum and warrants further study.
In conjunction with changes in potential composition of cellulosome and its release, increase in motility and signal transduction capability of the cells in stationary phase further highlights the evolution of this organism to feast and famine conditions in nature. If we assume that the cells release the cellulosomes in search of alternate nutrient sources, then it would be advantageous to correspondingly enhance the cells' ability to sense the oligomeric degradation products resulting from the activity of cellulosomes, although such mechanisms are currently unknown in this organism. Similarly, altering gene expression to improve cellular motility systems would help in appropriately orienting the cells' movement towards the nutrient gradient of interest. Hence the observed increase in expression of flagellar genes and chemotaxis genes is likely linked to adaptation and survival under famine conditions. Relatively little is understood about nutrient sensing mechanisms and the genes that are regulated in response to such senses in C. thermocellum. To our knowledge, this is the first global whole cell gene expression study in C. thermocellum, which enhances the current understanding of C. thermocellum physiological changes during cellulose fermentation and also lays the foundation for future studies with natural biomass.
The authors would like to thank Meghan Drake for assistance with qRT-PCR studies, and Brian Davison and Dale Pelletier for critically reviewing the manuscript and for providing valuable feedback. This work was sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory and through the BioEnergy Science Center (BESC). BESC is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle LLC for the U.S. D.O.E. under contract no. DE-AC05-00OR22725.
- Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS: Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 2002, 66 (3): 506-577. 10.1128/MMBR.66.3.506-577.2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Demain AL, Newcomb M, Wu JH: Cellulase, clostridia, and ethanol. Microbiol Mol Biol Rev. 2005, 69 (1): 124-154. 10.1128/MMBR.69.1.124-154.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Bayer EA, Belaich JP, Shoham Y, Lamed R: The cellulosomes: Multienzyme machines for degradation of plant cell wall polysaccharides. Annual Review of Microbiology. 2004, 58: 521-554. 10.1146/annurev.micro.57.030502.091022.PubMedView ArticleGoogle Scholar
- Fontes CM, Gilbert HJ: Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu Rev Biochem. 2010, 79: 655-681. 10.1146/annurev-biochem-091208-085603.PubMedView ArticleGoogle Scholar
- van Zyl WH, Lynd LR, den Haan R, McBride JE: Consolidated bioprocessing for bioethanol production using Saccharomyces cereviside. Biofuels. 2007, 108: 205-235. 10.1007/10_2007_061.View ArticleGoogle Scholar
- Lynd LR, van Zyl WH, McBride JE, Laser M: Consolidated bioprocessing of cellulosic biomass: an update. Current Opinion in Biotechnology. 2005, 16 (5): 577-583. 10.1016/j.copbio.2005.08.009.PubMedView ArticleGoogle Scholar
- Dror TW, Morag E, Rolider A, Bayer EA, Lamed R, Shoham Y: Regulation of the cellulosomal celS (cel48A) gene of Clostridium thermocellum is growth rate dependent. J Bacteriol. 2003, 185 (10): 3042-3048. 10.1128/JB.185.10.3042-3048.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y: Regulation of expression of scaffoldin-related genes in Clostridium thermocellum. J Bacteriol. 2003, 185 (17): 5109-5116. 10.1128/JB.185.17.5109-5116.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y: Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. J Bacteriol. 2005, 187 (7): 2261-2266. 10.1128/JB.187.7.2261-2266.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Mishra S, Beguin P, Aubert JP: Transcription of Clostridium thermocellum endoglucanase genes celF and celD. J Bacteriol. 1991, 173 (1): 80-85.PubMedPubMed CentralGoogle Scholar
- Stevenson DM, Weimer PJ: Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture. Appl Environ Microbiol. 2005, 71 (8): 4672-4678. 10.1128/AEM.71.8.4672-4678.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang YH, Lynd LR: Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum. J Bacteriol. 2005, 187 (1): 99-106. 10.1128/JB.187.1.99-106.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Carere CR, Kalia V, Sparling R, Cicek N, Levin DB: Pyruvate catabolism and hydrogen synthesis pathway genes of Clostridium thermocellum ATCC 27405. Indian Journal of Microbiology. 2008, 48 (2): 252-266. 10.1007/s12088-008-0036-z.PubMedPubMed CentralView ArticleGoogle Scholar
- Rydzak T, Levin DB, Cicek N, Sparling R: Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405. J Biotechnol. 2009, 140 (3-4): 169-175. 10.1016/j.jbiotec.2009.01.022.PubMedView ArticleGoogle Scholar
- Brown SD, Raman B, McKeown CK, Kale SP, He Z, Mielenz JR: Construction and evaluation of a Clostridium thermocellum ATCC 27405 whole-genome oligonucleotide microarray. Appl Biochem Biotechnol. 2007, 137-140 (1-12): 663-674. 10.1007/s12010-007-9087-6.PubMedView ArticleGoogle Scholar
- Raman B, Pan C, Hurst GB, Rodriguez M, McKeown CK, Lankford PK, Samatova NF, Mielenz JR: Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis. PLoS One. 2009, 4 (4): e5271-10.1371/journal.pone.0005271.PubMedPubMed CentralView ArticleGoogle Scholar
- Chhabra SR, He Q, Huang KH, Gaucher SP, Alm EJ, He Z, Hadi MZ, Hazen TC, Wall JD, Zhou J, Arkin AP, Singh AK: Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough. J Bacteriol. 2006, 188 (5): 1817-1828. 10.1128/JB.188.5.1817-1828.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, Li J, Thiagarajan M, White JA, Quackenbush J: TM4 microarray software suite. Methods Enzymol. 2006, 411: 134-193.PubMedView ArticleGoogle Scholar
- Sparling R, Islam R, Cicek N, Carere C, Chow H, Levin DB: Formate synthesis by Clostridium thermocellum during anaerobic fermentation. Can J Microbiol. 2006, 52 (7): 681-688. 10.1139/w06-021.PubMedView ArticleGoogle Scholar
- Islam R, Cicek N, Sparling R, Levin D: Influence of initial cellulose concentration on the carbon flow distribution during batch fermentation by Clostridium thermocellum ATCC 27405. Appl Microbiol Biotechnol. 2009, 82 (1): 141-148. 10.1007/s00253-008-1763-0.PubMedView ArticleGoogle Scholar
- Magnusson L, Cicek N, Sparling R, Levin D: Continuous hydrogen production during fermentation of alpha-cellulose by the thermophillic bacterium Clostridium thermocellum. Biotechnol Bioeng. 2009, 102 (3): 759-766. 10.1002/bit.22092.PubMedView ArticleGoogle Scholar
- Edgar R, Domrachev M, Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2002, 30 (1): 207-210. 10.1093/nar/30.1.207.PubMedPubMed CentralView ArticleGoogle Scholar
- Mao F, Dam P, Chou J, Olman V, Xu Y: DOOR: a database for prokaryotic operons. Nucleic Acids Res. 2009, D459-463. 37 DatabaseGoogle Scholar
- Roberts SB, Gowen CM, Brooks JP, Fong SS: Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production. BMC Syst Biol. 2010, 4: 31-10.1186/1752-0509-4-31.PubMedPubMed CentralView ArticleGoogle Scholar
- Lamed R, Zeikus JG: Ethanol production by thermophilic bacteria: relationship between fermentation product yields of and catabolic enzyme activities in Clostridium thermocellum and Thermoanaerobium brockii. J Bacteriol. 1980, 144 (2): 569-578.PubMedPubMed CentralGoogle Scholar
- Patni NJ, Alexander JK: Utilization of glucose by Clostridium thermocellum: presence of glucokinase and other glycolytic enzymes in cell extracts. J Bacteriol. 1971, 105 (1): 220-225.PubMedPubMed CentralGoogle Scholar
- Ozkan M, Yilmaz EI, Lynd LR, Ozcengiz G: Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization. Can J Microbiol. 2004, 50 (10): 845-851. 10.1139/w04-071.PubMedView ArticleGoogle Scholar
- Lynd LR, Grethlein HE, Wolkin RH: Fermentation of Cellulosic Substrates in Batch and Continuous Culture by Clostridium thermocellum. Appl Environ Microbiol. 1989, 55 (12): 3131-3139.PubMedPubMed CentralGoogle Scholar
- Shaw AJ, Hogsett DA, Lynd LR: Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout. J Bacteriol. 2009, 191 (20): 6457-6464. 10.1128/JB.00497-09.PubMedPubMed CentralView ArticleGoogle Scholar
- Zverlov VV, Kellermann J, Schwarz WH: Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes. Proteomics. 2005, 5 (14): 3646-3653. 10.1002/pmic.200401199.PubMedView ArticleGoogle Scholar
- Gold ND, Martin VJ: Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. J Bacteriol. 2007, 189 (19): 6787-6795. 10.1128/JB.00882-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Newcomb M, Chen CY, Wu JH: Induction of the celC operon of Clostridium thermocellum by laminaribiose. Proc Natl Acad Sci USA. 2007, 104 (10): 3747-3752. 10.1073/pnas.0700087104.PubMedPubMed CentralView ArticleGoogle Scholar
- Kahel-Raifer H, Jindou S, Bahari L, Nataf Y, Shoham Y, Bayer EA, Borovok I, Lamed R: The unique set of putative membrane-associated anti-sigma factors in Clostridium thermocellum suggests a novel extracellular carbohydrate-sensing mechanism involved in gene regulation. FEMS Microbiol Lett. 2010, 308 (1): 84-93. 10.1111/j.1574-6968.2010.01997.x.PubMedView ArticleGoogle Scholar
- Bayer EA, Setter E, Lamed R: Organization and distribution of the cellulosome in Clostridium thermocellum. J Bacteriol. 1985, 163 (2): 552-559.PubMedPubMed CentralGoogle Scholar
- Strobel HJ, Caldwell FC, Dawson KA: Carbohydrate Transport by the Anaerobic Thermophile Clostridium thermocellum LQRI. Appl Environ Microbiol. 1995, 61 (11): 4012-4015.PubMedPubMed CentralGoogle Scholar
- Nataf Y, Yaron S, Stahl F, Lamed R, Bayer EA, Scheper TH, Sonenshein AL, Shoham Y: Cellodextrin and laminaribiose ABC transporters in Clostridium thermocellum. J Bacteriol. 2009, 191 (1): 203-209. 10.1128/JB.01190-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang YH, Lynd LR: Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation. Proc Natl Acad Sci USA. 2005, 102 (20): 7321-7325. 10.1073/pnas.0408734102.PubMedPubMed CentralView ArticleGoogle Scholar
- Shi Z, Blaschek HP: Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 and the hyper-butanol-producing mutant BA101 during the shift from acidogenesis to solventogenesis. Appl Environ Microbiol. 2008, 74 (24): 7709-7714. 10.1128/AEM.01948-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Alsaker KV, Papoutsakis ET: Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. J Bacteriol. 2005, 187 (20): 7103-7118. 10.1128/JB.187.20.7103-7118.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Bioenergy Research Centers: An Overview of the Science. US Department of Energy. 2009, DOE/SC-0116Google Scholar
- Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B: The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2009, D233-238. 37 DatabaseGoogle Scholar
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